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Offlinefrankwhite85
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Registered: 04/18/16
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considered slow growth on agar?
    #23898817 - 12/06/16 01:48 AM (7 years, 1 month ago)

I use an agar mix of 6g lme to 12 g agar (to promote rhizo growth) and 600 ml distilled waterpc'd for 45 min then poured in front of a floowhood.  Its generally taking my cultures 6-7 days to grow to the size of about a half dollar that's when i can start transferring sectors.  I use three section petris because its easy to gauge when you should start transferring.  Is that slow? whats optimum?


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OfflineA.RichardTrickle
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Re: considered slow growth on agar? [Re: frankwhite85]
    #23898820 - 12/06/16 01:50 AM (7 years, 1 month ago)

:takingnotes:


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[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
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InvisibleNobler Hino
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Re: considered slow growth on agar? [Re: A.RichardTrickle]
    #23898932 - 12/06/16 04:10 AM (7 years, 1 month ago)

Thats pretty normal, 6 days to grow out a transfer is ok.

Edit* should be the size of a half dollar by then

I would bump your pc time up to an hour, 45 mins is the bare minimum.


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"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


Edited by Nobler Hino (12/06/16 04:20 AM)


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Offlineenlightenment
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Re: considered slow growth on agar? [Re: Nobler Hino]
    #23898996 - 12/06/16 05:28 AM (7 years, 1 month ago)

Quote:

Nobler Hino said:
Thats pretty normal, 6 days to grow out a transfer is ok.





True


Quote:

Nobler Hino said:
I would bump your pc time up to an hour, 45 mins is the bare minimum.




False.
20 minutes at 15 PSI is plenty for MEA.
You want to prevent Maillard reaction. I would not PC longer than 30 minutes.


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OfflineWeavieWonder
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Re: considered slow growth on agar? [Re: enlightenment]
    #23899030 - 12/06/16 06:08 AM (7 years, 1 month ago)

6-7 days for a transfer to grow that size is good, considering it spends a day or two recovering from the transfer.


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InvisibleNobler Hino
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Re: considered slow growth on agar? [Re: WeavieWonder]
    #23899213 - 12/06/16 08:04 AM (7 years, 1 month ago)

Pasty does his at 40-45, rr's video says 45.

Maybe an hour is overkill, ill have to read up on the maillard reaction, thanks and my apologies.


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


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OfflineAlCapone2k
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Re: considered slow growth on agar? [Re: Nobler Hino]
    #23899389 - 12/06/16 09:37 AM (7 years, 1 month ago)

I PC my chinese store agar poweder + powdery LME for 20 minutes without any contamn issue :smile:

Watery solutions can be sterilized less than 30 minutes without any problems.


Regards


--------------------
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Offlinefrankwhite85
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Re: considered slow growth on agar? [Re: Nobler Hino]
    #23900893 - 12/06/16 06:38 PM (7 years, 1 month ago)

I Pc for at least 45 min because i'm at a higher altitude I've read you need to cook longer the higher up you are.


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OfflineAlCapone2k
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Re: considered slow growth on agar? [Re: frankwhite85]
    #23900930 - 12/06/16 06:49 PM (7 years, 1 month ago)

I can't say for sure but since the pressure in the PC is independent from the armospheric pressure, I can't imagine that it makes a different.


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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OfflinecronicrFacebook
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Re: considered slow growth on agar? [Re: Nobler Hino]
    #23900979 - 12/06/16 07:04 PM (7 years, 1 month ago)

Quote:

Nobler Hino said:
Pasty does his at 40-45, rr's video says 45.

Maybe an hour is overkill, ill have to read up on the maillard reaction, thanks and my apologies.



Watched again...he does say 45..i have always done 30 but also this is from me slowly droping the pressure


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OfflineKenetic
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Re: considered slow growth on agar? [Re: cronicr]
    #23901106 - 12/06/16 07:35 PM (7 years, 1 month ago)

I do 30 @ 10 psi


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InvisibleMad Season
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Re: considered slow growth on agar? [Re: Kenetic]
    #23901852 - 12/06/16 11:23 PM (7 years, 1 month ago)

20 mins @18, for oat water agar, MEA, and DFA.


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