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OfflineCthxath
I'm a teapot

Registered: 12/05/16
Posts: 11
Last seen: 6 years, 11 months
Absolute Beginner - Incubation Question *DELETED*
    #23897635 - 12/05/16 05:49 PM (7 years, 1 month ago)

no


Edited by Cthxath (02/05/17 08:12 PM)


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OfflinePsilocyBen17
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Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
Re: Absolute Beginner - Incubation Question [Re: Cthxath]
    #23897718 - 12/05/16 06:18 PM (7 years, 1 month ago)

are you using the standard brf recipe? Looks bacterial to me.


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OfflineCthxath
I'm a teapot


Registered: 12/05/16
Posts: 11
Last seen: 6 years, 11 months
Re: Absolute Beginner - Incubation Question [Re: PsilocyBen17]
    #23897736 - 12/05/16 06:21 PM (7 years, 1 month ago)

Each jar has the following ratio:
140 ml vermiculite
40 ml BRF


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Invisibleegobrain


Registered: 11/17/16
Posts: 180
Loc: Canada Flag
Re: Absolute Beginner - Incubation Question [Re: Cthxath]
    #23897743 - 12/05/16 06:24 PM (7 years, 1 month ago)

All I see is bacteria. :shrug:

The mycelium growth should be spreading out evenly from the inoculation points. Assuming you used 4 sites, there should be 4 areas showing growth. It looks like the growth is coming from the bottom, indicating that the jars are too wet and bacteria is taking advantage of that. Also, temps getting into the 80s will be favouring bacterial growth. Its not actually necessary to incubate at high temps. Room temp is fine.


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OfflineCthxath
I'm a teapot


Registered: 12/05/16
Posts: 11
Last seen: 6 years, 11 months
Re: Absolute Beginner - Incubation Question [Re: egobrain]
    #23897771 - 12/05/16 06:30 PM (7 years, 1 month ago)

Perhaps I will let these jars do their thing for a while for the experience in observing "stuff" grow. If it is bacterial it might be a good experience for future endeavors.


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OfflineChakatron
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Registered: 07/09/13
Posts: 1,646
Loc: Your Face Flag
Last seen: 3 months, 19 days
Re: Absolute Beginner - Incubation Question [Re: Cthxath]
    #23897787 - 12/05/16 06:35 PM (7 years, 1 month ago)

Those must smell pretty bad already, keeping those around is no bueno if you plan on going at it again.


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Invisibleegobrain


Registered: 11/17/16
Posts: 180
Loc: Canada Flag
Re: Absolute Beginner - Incubation Question [Re: Cthxath]
    #23897807 - 12/05/16 06:39 PM (7 years, 1 month ago)

I think you've seen all you need to see from these jars.

What you should be doing is breaking down and analyzing everything you did during the inoculation of these jars in order to figure out why they didn't work out.

Did you PC these jars? If you put too much water in the cooker, it could have ended up in the jars. The moisture content is very important. Also, if you didn't let the jars/sub cool to room temp the spores could have been killed upon injection.


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OfflineCthxath
I'm a teapot


Registered: 12/05/16
Posts: 11
Last seen: 6 years, 11 months
Re: Absolute Beginner - Incubation Question [Re: Chakatron]
    #23897869 - 12/05/16 06:51 PM (7 years, 1 month ago)

No odor at all. I would open one up and smell it directly but I'd bet that if it's a particularly nasty contaminant sniffing it would be a severe no-no.


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OfflineCthxath
I'm a teapot


Registered: 12/05/16
Posts: 11
Last seen: 6 years, 11 months
Re: Absolute Beginner - Incubation Question [Re: Cthxath]
    #23898452 - 12/05/16 10:05 PM (7 years, 1 month ago)

Update:
As egobrain pointed out, I likely had too much moisture in my jars.
Too bad I didn't write stuff down a few weeks ago to compare with future attempts. I just re-did substrate preparation and currently have 6 fresh jars in the pressure cooker. I feel that my fresh substrate has a better coating of BRF and has a more airy consistency. It very clearly is damp but not so much as like the first attempt that the liquid would condense to the bottom of my jar. Also the verm layer is much more compact and with the lid on top has basically no wiggle room outside of somebody violently shaking the jar (which will not happen during incubation of course). I also will be using micropore tape for this batch as an added layer of filtration. Will inoculate tomorrow morning after the jars have cooled and I take a shower and stuff. The particular strain is Malabar.


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InvisibleShroomerInTheRye
Clit Commander
I'm a teapot User Gallery


Registered: 01/12/12
Posts: 13,036
Loc: Themyscira Flag
Re: Absolute Beginner - Incubation Question [Re: Cthxath] * 1
    #23898491 - 12/05/16 10:21 PM (7 years, 1 month ago)

Whatever you do, I would not open those jars inside.


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