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InvisibleComebackKid
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Paranoid about white mold? Agar identification
    #23894874 - 12/04/16 07:00 PM (7 years, 1 month ago)

So Ivery been having trouble germinating KSSS spores for months now.
Just tried brf paste and have had mycelium growth from where I dropped spores.
When I moved it to agar the growth was uniform but very wispy and unfamiliar looking so I tossed it.

Wondering if I'm being too paranoid because I now have more plates with a tiny bit thicker but still wispy myc. I will make a transfer and take a pic of it tomorrow. But here's a pic of the plate I tossed.


Just wanna run these by shroomery before tossing out of paranoia and wasting another months work.
I appreciate any advice!


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

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InvisibleMoabfighter
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Re: Paranoid about white mold? Agar identification [Re: ComebackKid] * 1
    #23894889 - 12/04/16 07:06 PM (7 years, 1 month ago)

Is that z strain? Every single one of my z strain plates (atleast 5) look like that. Transferred, from spores, all of them. Not sure why.

I've used a plate similar to the one pictured, and it seems to be growing fine.



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KSSS And PE WBS.
 


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InvisibleMoabfighter
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Re: Paranoid about white mold? Agar identification [Re: Moabfighter]
    #23894903 - 12/04/16 07:10 PM (7 years, 1 month ago)

Here ya go. In real life this one looks exactly as yours does.



Edit. I see it's KSSS. Mine from the same person I got real lucky with. Few germinated. I made a lot of just KSSS plates because I was excited for it. I'd say only 5/16 germinated.

Hell only 1/5 transfers latched on. Not sure what's up with it. Could be my agar recipe.


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KSSS And PE WBS.
 


Edited by Moabfighter (12/04/16 07:13 PM)


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InvisibleComebackKid
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Re: Paranoid about white mold? Agar identification [Re: Moabfighter]
    #23894909 - 12/04/16 07:11 PM (7 years, 1 month ago)

Supposed to be KSSS
but I really don't like the look of the mycelium. I'm afraid it's mold because all my other mycelium is thick and bright white.
My first plate I ever had [pic]

had similar looking mycelium that turned green before full colonization of the plate. But the growth was not uniform like it is here.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
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InvisibleComebackKid
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Re: Paranoid about white mold? Agar identification [Re: Moabfighter]
    #23894923 - 12/04/16 07:16 PM (7 years, 1 month ago)

True. I may just be paranoid. Seems to be a common theme with me :lol:
I'll make a transfer and take a whiff of the plate after.
Ill post a pic with the verdict.
Thanks moab!


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

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InvisiblePastywhyteMDiscord
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Re: Paranoid about white mold? Agar identification [Re: ComebackKid] * 2
    #23894929 - 12/04/16 07:18 PM (7 years, 1 month ago)

Quote:

ComebackKid said:
So Ivery been having trouble germinating KSSS spores for months now.
Just tried brf paste and have had mycelium growth from where I dropped spores.
When I moved it to agar the growth was uniform but very wispy and unfamiliar looking so I tossed it.

Wondering if I'm being too paranoid because I now have more plates with a tiny bit thicker but still wispy myc. I will make a transfer and take a pic of it tomorrow. But here's a pic of the plate I tossed.


Just wanna run these by shroomery before tossing out of paranoia and wasting another months work.
I appreciate any advice!




Definitely mold. I'm going with pin mold.


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InvisibleComebackKid
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Re: Paranoid about white mold? Agar identification [Re: Pastywhyte]
    #23894971 - 12/04/16 07:34 PM (7 years, 1 month ago)

Thanks pasty
I have two other plates with different but still questionable myc. I'll take pictures after I transfer cause it's no use with this cameras auto focus right now.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleMoabfighter
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Re: Paranoid about white mold? Agar identification [Re: ComebackKid]
    #23895016 - 12/04/16 07:48 PM (7 years, 1 month ago)

Hell CBK.

Wasn't trying to misinform. Guess I have pinmold too


--------------------
KSSS And PE WBS.
 


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InvisibleComebackKid
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Re: Paranoid about white mold? Agar identification [Re: Moabfighter]
    #23895034 - 12/04/16 07:53 PM (7 years, 1 month ago)

It's cool man. Not like I Inoculated grains with it or anything:lol:
If the plates don't smell like mushrooms after I make transfers then I'm not going to fuck with that myc anymore anyway. Seems like a good rule of thumb for anything questionable. :shrug:

I hope you didn't use that type of mycelium on your WBS


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleComebackKid
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Re: Paranoid about white mold? Agar identification [Re: ComebackKid]
    #23898121 - 12/05/16 08:05 PM (7 years, 1 month ago)

OkaY so I'm pretty sure this is pin mold now that I can take a good look at it.
This was transfered from brf paste to agar.



This is another brf plate. Looks a little more promising but still questionable to me.



I just took a couple transfers to agar. Hoping the growth stays like this and doesn't thin out like the pin mold plates.
I gave it a whiff after I took transfers but all I could smell was brf smell :sad:

What do you guys think?


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Re: Paranoid about white mold? Agar identification [Re: ComebackKid] * 1
    #23898157 - 12/05/16 08:13 PM (7 years, 1 month ago)

Pic two looks fine


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Re: Paranoid about white mold? Agar identification [Re: cronicr]
    #23898225 - 12/05/16 08:40 PM (7 years, 1 month ago)

FUCK YES! Thanks Cron! I was losing hope on these spores. PF cakes haven't even shown signs of life yet and they're almost 3 weeks old

Phew! Germinating spores can be quite a challenge


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleComebackKid
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Re: Paranoid about white mold? Agar identification [Re: ComebackKid]
    #23944093 - 12/20/16 05:26 PM (7 years, 1 month ago)

Apologies for bumping this old thread but could really use your guys' guidance.

Is it possible that plate was just pin mold in disguise because of the highly nutritious BRF paste?
Or did I just fuck up hard with my transfers?

This is what I'm working with now once I moved to agar :sad:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisiblePastywhyteMDiscord
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Re: Paranoid about white mold? Agar identification [Re: ComebackKid]
    #23944114 - 12/20/16 05:34 PM (7 years, 1 month ago)

That is definitely mold. Media like brf paste can result in different phenotype for contams. In fact some species of mold are identifiable based on their appearance on certain kinds of media.


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InvisibleComebackKid
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Re: Paranoid about white mold? Agar identification [Re: Pastywhyte]
    #23944138 - 12/20/16 05:43 PM (7 years, 1 month ago)

Either way I was screwed but still good to know. I really appreciate all the help guys. Thanks for sharing your expertise


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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