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InvisibleMycolorado
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Chicon Nindo and tampanensis (stones and fruit)
    #23892271 - 12/03/16 10:07 PM (7 years, 1 month ago)

Guess I'll throw out some pics as well.  Here's some CN and tamp cultures I'm working on.


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OfflineFerather
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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23892939 - 12/04/16 05:41 AM (7 years, 1 month ago)

Are the brown ones black tea? They look identical in speed, the TA looks more populated (less gaps) on the brown ones.


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Re: Chicon Nindo and tampanensis [Re: Ferather]
    #23893063 - 12/04/16 07:50 AM (7 years, 1 month ago)

Maybe it's coffee because coffee soaked grains seem to improve the production of sclerotia. Never tried coffee dishes. Stones do not develop on my plates for some reason... After inoculation stones start to to develop in the jars but even on very old dishes I don't have stones.

Maybe I should try to add coffee to the agar.
I don't know if it's coffee or tea but anyway..
:threadmonitor:


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OfflineFerather
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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23893088 - 12/04/16 07:59 AM (7 years, 1 month ago)

Nvm, its probs coffee, the first set says "cafe" at the bottom.

:takingnotes:


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23893113 - 12/04/16 08:14 AM (7 years, 1 month ago)

Yep, coffee (weak coffee MEA).  I made them up as it was mentioned on here that it promotes stones.  They are moving a tad slower than the straight MEA plates...my guess is due to more nutes in the coffee.  Unfortunately, the phone camera doesn't come close to doing these justice...they're beautiful!  The tamps developed from a single colony and the CN plate had 3...a couple are producing stones.  Colony 3 produced a stone right off the bat...I'll transfer it soon.  I might try some plates with silica added to see if that has any effect on stone production. Here's the stone plate.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23893122 - 12/04/16 08:18 AM (7 years, 1 month ago)

Quote:

my guess is due to more nutes in the coffee




Too much coffee slows the mycelium growth. I noticed this by soaking rye in too strong coffee. Coffee is acidic that might be the reason for the slow growth if you use too much.


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23893159 - 12/04/16 08:39 AM (7 years, 1 month ago)

GTK.  I diluted it to decrease the nute content but also figured it might have similar inhibitory effects as the tea so didn't want to use strong coffee.  At this point, I'm not seeing any noticeable difference with the exception of speed which is minor.  I read that sand is beneficial in or used solely as the casing when it comes to fruiting and was curious if silica will have any effect on stone production as well. Gonna test some silica plates soon and will share those results. Morels produce sclerotia and I often find those in sandy soil...


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23893172 - 12/04/16 08:46 AM (7 years, 1 month ago)

Quote:

enlightenment said:
Quote:

my guess is due to more nutes in the coffee




Too much coffee slows the mycelium growth. I noticed this by soaking rye in too strong coffee. Coffee is acidic that might be the reason for the slow growth if you use too much.



Same issue with lots of tea, too acidic, if you want more add lime to balance.


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23893185 - 12/04/16 08:50 AM (7 years, 1 month ago)

:takingnotes::popcorn::thumbup:


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Re: Chicon Nindo and tampanensis [Re: wtfcrazymofo]
    #23893245 - 12/04/16 09:24 AM (7 years, 1 month ago)

Catsan, or similar lime products, notice calcium carbonate (lime) is used for antibacterial reasons.
Anyway point being it can be used as grit, or direct casing maybe, don't know.

Side note, limed tea (Ph 6) is a bit of a bacteria bomb.


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Re: Chicon Nindo and tampanensis [Re: Ferather]
    #23899445 - 12/06/16 10:06 AM (7 years, 1 month ago)

The donor tamp plate is starting to form stones at 1, 6 and 8 o'clock.  Pretty rapid development as I didn't notice these yesterday.  Also looks like some pins might be forming around the perimeter of initial growth. 


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23913314 - 12/10/16 02:32 PM (7 years, 1 month ago)

Transferred some stones from the original tamp and CN plates.  This was the larger of the 2 CN stones transferred...it was dense!

The tamp stone carried a little more mycelium than I cared for but oh well.

Here's the nicest looking tamp transfer starting to form a stone.

A good looking variety of cultures going to grain


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23915877 - 12/11/16 10:21 AM (7 years, 1 month ago)

Quote:

enlightenment said:
Stones do not develop on my plates for some reason...:



I was just looking over TMC and it says stones form better in total darkness.  My plates are forming stones quite readily and are kept in the dark.  It also states that stone production on plates was highest at 4.5% MEA.  Wanted to throw that out in case you hadn't seen it.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23915891 - 12/11/16 10:27 AM (7 years, 1 month ago)

TY. Today I cloned some Psi galindoi stones to agar.



I used 10g LME for 500ml water.
That's my standard agar recipe. I use it with everything. Sometimes I make a batch of PDA dishes.

Let's see how the dishes grow.


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23950000 - 12/22/16 07:49 PM (7 years, 1 month ago)

Stone plates are looking good. The smaller of the CN stones has put out nice, quick, uniform growth but unfortunately has overgrown a small bac colony.  The bigger one hasn't grown out as much but looks like it may be startting a new stone.

The original tamp plate keeps developing stones...will continue to harvest and start new plates till it dries up.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23957743 - 12/26/16 06:47 AM (7 years, 1 month ago)

Quote:

Mycolorado said:
Quote:

enlightenment said:
Stones do not develop on my plates for some reason...:



I was just looking over TMC and it says stones form better in total darkness.  My plates are forming stones quite readily and are kept in the dark.  It also states that stone production on plates was highest at 4.5% MEA.  Wanted to throw that out in case you hadn't seen it.





Merry Xmas Myc

If I was going to use PADY would you try 4.5 % with this?  I dont have any Malt atm


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Re: Chicon Nindo and tampanensis [Re: Edmunter]
    #23957849 - 12/26/16 08:39 AM (7 years, 1 month ago)

I'd say give it a try, though I really couldn't speculate as I've only run them on 3% MEA...I'm gonna try them out on DFA, prolly 3% as well, to see how they do on it.  I haven't gone up to 4.5% yet as the cultures I'm working with are producing stones fine as is...may just be good genetics, which is great cause these prints came from an awesome shroomerite.  Here's a tamp jar just getting going and would appear stones are already beginning to develop.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23960463 - 12/27/16 12:42 PM (7 years, 1 month ago)

Ive taken 1 pin from this plate already, im going to take another but do you see any stones or areas that look decent for transfer for stones?



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Re: Chicon Nindo and tampanensis [Re: Edmunter]
    #23960569 - 12/27/16 01:37 PM (7 years, 1 month ago)

Looks like some stones forming in a couple of those pics unless they're knots.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23960574 - 12/27/16 01:39 PM (7 years, 1 month ago)

Its one plate.  Are the small white knot like areas stones you think.  There are browning areas too.  Im transferring tomorrow so be good to get it right


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Re: Chicon Nindo and tampanensis [Re: Edmunter]
    #23960870 - 12/27/16 04:02 PM (7 years, 1 month ago)

Shot 3 looks like a stone just up and right of center.  I'd dig that up and transfer it as well as any area showing the darkest brown hard growth visible from the bottom of the plate.  So far the CN stones grow under the surface like your plate appears to be doing and the tamp stones grow on top.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23960950 - 12/27/16 04:44 PM (7 years, 1 month ago)

I was given a print by azure when he and elfstone first released CN Mex.  I never had alot of luck with stones from it.  P. Tamp has always been hit and miss for stone production for me also.  Sometimes ill get 200g wet after 3 months sometimes 30-40 from MS :shrug:  You just seem to never know with stone producers with the exception of P. Galindoi large sclerotia.  If you want stones id suggest doing the agar work on that species


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Re: Chicon Nindo and tampanensis [Re: olskoolgrower]
    #23961007 - 12/27/16 05:22 PM (7 years, 1 month ago)

I've been transferring the stones with the goal of propagating those traits.  Hopefully, it pays off, though I'm not sure how much nature v nurture plays in stone formation.  My gut is leaning toward nurture.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23961015 - 12/27/16 05:26 PM (7 years, 1 month ago)

Quote:

Mycolorado said:
I've been transferring the stones with the goal of propagating those traits.  Hopefully, it pays off, though I'm not sure how much nature v nurture plays in stone formation.  My gut is leaning toward nurture.



Get some different stone producer MS syringes and grow them under the same conditions.  Im sure that after that you will change your mind...


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Re: Chicon Nindo and tampanensis [Re: olskoolgrower]
    #23961022 - 12/27/16 05:29 PM (7 years, 1 month ago)

excellent!  Post's like these really make me want to try some stones for sure.  And throughout my whole agar career I've never had a plate fruit on me.  Really surprised you got some stones!

:popcorn:


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Re: Chicon Nindo and tampanensis [Re: olskoolgrower]
    #23961101 - 12/27/16 06:20 PM (7 years, 1 month ago)

These were started from prints with 3 CN colonies developing on a plate and 1 tamp colony on another.  At this point, all 3 CN colonies have produced at least one stone and the tamp colony has produced 3 large stones.  I have no experience with stone producers so nothing practical to base the assumption on but Stamets says stone production is best on 4.5% MEA grown in the dark. That with the fact that sclerotia are a strategy for surviving unfavorable conditions makes me lean towards nurture.  Then again, maybe I was fortunate and got good stone-producing genetics.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23961109 - 12/27/16 06:26 PM (7 years, 1 month ago)

well you're doing something right for sure


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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #23961756 - 12/28/16 01:33 AM (7 years, 1 month ago)

Quote:

kenetic said:
excellent!  Post's like these really make me want to try some stones for sure.  And throughout my whole agar career I've never had a plate fruit on me.  Really surprised you got some stones!

:popcorn:




Thats strange a nearly every plate I have ,if clean ,fruits at some point if I just leave it


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Re: Chicon Nindo and tampanensis [Re: Edmunter]
    #23961770 - 12/28/16 01:46 AM (7 years, 1 month ago)

Definitely something going on under the surface



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Re: Chicon Nindo and tampanensis [Re: Edmunter]
    #23962195 - 12/28/16 08:54 AM (7 years, 1 month ago)

Do you use petris or pastybplates edmunter


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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #23962204 - 12/28/16 09:01 AM (7 years, 1 month ago)

Pasty easys


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Re: Chicon Nindo and tampanensis [Re: Edmunter]
    #23962557 - 12/28/16 11:41 AM (7 years, 1 month ago)

Invitro shrooms seem to love those pasty plates because of all the extra air and room inside, makes a nice little microclimate, but petris don't have all the room and air and stuff.  My agar dries before I see knots.  Some people get invitro on plates but Im not sure how.  Maybe I just havent kept them long enough


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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #23962570 - 12/28/16 11:49 AM (7 years, 1 month ago)

Its the quickest way to get a decent growing culture IMO.  Spores plate-pin-plate-fruit.  I also take transfers from the original plate too.


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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #23962571 - 12/28/16 11:49 AM (7 years, 1 month ago)





Usually I see pins after 2 weeks of full colonization of the agar dish.
(not on every dish. About 20% of cubensis dishes produce invitro pins IME)

I use parafilm. It prevents the agar from drying too fast. Maybe this is the reason. :shrug:

If you use parafilm, too. I don't know why you don't get invitro pins.


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23962592 - 12/28/16 11:56 AM (7 years, 1 month ago)

Who knows.  Not a big priority for me anyway besides the novelty aspect.


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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #23964836 - 12/29/16 07:55 AM (7 years, 1 month ago)

Looks great!


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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #23964898 - 12/29/16 08:29 AM (7 years, 1 month ago)

Quote:

kenetic said:
Who knows.  Not a big priority for me anyway besides the novelty aspect.




But t is a way to get a sterile pin with all the genetics you need for fruiting and growing fast.


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Re: Chicon Nindo and tampanensis [Re: Edmunter]
    #23964905 - 12/29/16 08:37 AM (7 years, 1 month ago)

I agree that there are quite a few advantages, including less cell division but I have enough going on that I am not pressed for time nor do I have any issues with taking biopsy samples for cloning. 
I'd rather just fruit a small ms grow and clone from there.


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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #23964908 - 12/29/16 08:39 AM (7 years, 1 month ago)

Fair play, I do both. For certain species that are hard to fruit I find this gives you a headstart


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Re: Chicon Nindo and tampanensis [Re: Edmunter]
    #23964912 - 12/29/16 08:40 AM (7 years, 1 month ago)

I have taken 2 stones from my original spore plate now and transferred them and placed them in the dark.


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Re: Chicon Nindo and tampanensis [Re: Edmunter] * 1
    #23968954 - 12/30/16 07:18 PM (7 years, 1 month ago)

looks like the first stones are gonna start here. This is a tamp jar...the same type growth led to stones on agar. 


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Re: Chicon Nindo and tampanensis [Re: Mycolorado] * 1
    #23970238 - 12/31/16 09:23 AM (7 years, 30 days ago)

Here's a new stone forming on a stone transfer plate.  This is the plate with the small bacteria colony (visible in the second pic as a clear spot).  The circled dark spot is the new stone.  As I posited on ed's thread, I think bacteria may be the key to triggering or is at least, very influential on stone formation.  Could also just be the physical obstruction on the surface of the agar allowing for an anchor point from which the stone can grow.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23972747 - 01/01/17 11:21 AM (7 years, 29 days ago)



Sorry I didn't try for a better picture yet, but here's my tamp plates.
From right to left -> rightmost plate: inoc'd on 11-21 with a MS syringe; 2nd & 3rd from right: transfers from original plate; 4th & 5th from right: each with a slice of one of a stone from the first transfer plate.

this is my first experience with sclerotia producing stuffs, I really didn't do anything special to them that I know of. I had no idea they don't typically grow stones right on the plate D=


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Edited by Aneres (01/01/17 11:22 AM)


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Re: Chicon Nindo and tampanensis [Re: Aneres]
    #23973636 - 01/01/17 05:37 PM (7 years, 29 days ago)

Nice!  So you had a transfer plate produce more stones?  I' debated slicing them but decided against it as I had several to use.  Looks like a pretty beefy stone in the second from the right!


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23973731 - 01/01/17 06:00 PM (7 years, 29 days ago)

Quote:

Mycolorado said:
Nice!  So you had a transfer plate produce more stones?  I' debated slicing them but decided against it as I had several to use.  Looks like a pretty beefy stone in the second from the right!




Actually the transfers grew stones long before the original did. I very honestly had (have, heh) no idea how exactly one is supposed to be working with these, I admit i've just been winging it and treating them the same as the rest of my cultures. It was just a random choice really, to cut the stone (into three pieces, one of which remained in situ). I like to do two of everything if possible in case of contams, so the two leftmost plates in that pic are from the same stone.

One thing to note which I don't know if is significant or not - the three rightmost plates were originally dyed green with food coloring but the myc turned it back to a very pale amber


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Re: Chicon Nindo and tampanensis [Re: Aneres]
    #23979793 - 01/03/17 07:44 PM (7 years, 27 days ago)

Progress pic of the first stone with a second starting.  Not crazy explosive growth but still impressive considering the jar is only a third-ish colonized.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23982332 - 01/04/17 07:25 PM (7 years, 26 days ago)

:rockon:


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https://www.shroomery.org/forums/showflat.php/Number/28622922




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Re: Chicon Nindo and tampanensis [Re: wtfcrazymofo]
    #23982338 - 01/04/17 07:28 PM (7 years, 26 days ago)

that istan vat izz up
That is just how daddy likes it


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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #24000281 - 01/11/17 05:45 AM (7 years, 19 days ago)

It suddenly occurs to me that perhaps the reason I got stones when others don't may be because my tamp plates were started on like 12-9 and thus overgrown


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Re: Chicon Nindo and tampanensis [Re: Aneres]
    #24005061 - 01/12/17 06:17 PM (7 years, 18 days ago)

Yeah, it was 3-4 weeks for the CN stone and around 5 weeks for the tamp stones to start on agar...may see the same pattern in the jars.  These are right around 4 weeks and spent the first two at about 69F, so moved pretty slowly.  Anyway, the 4 on the left were shaken.  3 CN (left) and 3 tamps.  The far right jar is the one with the small stone on the bottom.  All the tamp jars look like they may be getting ready to start stoning.



This jar came from the nicest tamp culture and is starting to produce a lot of that dark brown mycelium.  Hope that's an indicator that it's gonna do...something?  Different angles of same jar:



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24011794 - 01/15/17 10:27 AM (7 years, 15 days ago)

Tamp stone...super slow growth. 



Here's a shaken CN jar.  Some small stones forming.



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24019795 - 01/18/17 12:03 PM (7 years, 12 days ago)

CN stone plate...appears that the stone has grown a new lobe...hard to tell from the pic and all the metabolites covering it.  Also, it's producing a new stone at 1 o'clock.



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24028951 - 01/21/17 06:58 PM (7 years, 9 days ago)

CN stones forming in all 3 shaken jars.  They have more of a grey color than the tamps which are more yellow.  I'm going to use the above stone plate to make a LI for a couple jars tomorrow.  Hopefully the isolation and additional water will help them move quicker and stone up better.  Side note, I'm using wheat so not sure if that's effecting stone formation.  I'll run rye on the next round. 



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24028964 - 01/21/17 07:05 PM (7 years, 9 days ago)



--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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Re: Chicon Nindo and tampanensis [Re: wtfcrazymofo]
    #24028990 - 01/21/17 07:20 PM (7 years, 9 days ago)

Thanks, wtf!  stonesun's shit rocks.  I'm not totally sold on the coffee but will give it a shot.  I think the LI is really going to help.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24029016 - 01/21/17 07:39 PM (7 years, 9 days ago)

The best smell of any grain I've prepped.


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Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




Edited by wtfcrazymofo (01/21/17 07:41 PM)


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Re: Chicon Nindo and tampanensis [Re: wtfcrazymofo]
    #24031717 - 01/22/17 07:51 PM (7 years, 8 days ago)

Made 100ml of LI from the CN stone plate and inoculated 4 qts. of rye. May be stretching it.  I transferred the stone which is growing and forming more stones.  Here are a couple different angles. Sorry for the fuzzy second pic.



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24031880 - 01/22/17 08:39 PM (7 years, 8 days ago)

:hi:
nice stuff
surprised I didn't notice this thread floating around


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Re: Chicon Nindo and tampanensis [Re: bodhisatta]
    #24031972 - 01/22/17 09:12 PM (7 years, 8 days ago)

Thanks, bodh!


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24043701 - 01/27/17 12:47 PM (7 years, 3 days ago)

This jar received the majority of LI and is just starting to recover.  Looks like it'll take right off.  Yays LI!



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24047335 - 01/28/17 07:36 PM (7 years, 2 days ago)

Some nice stone development in this CN jar. 



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24053654 - 01/31/17 07:30 AM (6 years, 11 months ago)

Another of the CN jars forming tons of small stones. 



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24060362 - 02/02/17 02:32 PM (6 years, 11 months ago)

Getting stone development next to some agar chunks in these 2 CN jars nine days after LI using the best stone plate culture.  Pretty promising. 



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24060516 - 02/02/17 03:43 PM (6 years, 11 months ago)

Looks like a cool grow.  The only thing that turns me off from stone producers is the thought of bacterial jars.  Endospores seem impossible to eliminate completely and they always manage to grow at some point. 

A constant supply of fresh mushies sounds pretty good though


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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #24062650 - 02/03/17 12:52 PM (6 years, 11 months ago)

Quote:

kenetic said:
Looks like a cool grow.  The only thing that turns me off from stone producers is the thought of bacterial jars.  Endospores seem impossible to eliminate completely and they always manage to grow at some point. 

A constant supply of fresh mushies sounds pretty good though




However if all the nutritious area is covered with mycellium, endospores have nowhere to germinate.


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Re: Chicon Nindo and tampanensis [Re: Edmunter]
    #24062861 - 02/03/17 02:52 PM (6 years, 11 months ago)

My worries are that they will germinate before the jar is colonized and grow with the myc.  Nothing a dehydrator can't fix I guess, ultimately the small amounts of bacteria I experience (if that's even what it is) isn't harmful and after you dehydrate your fruits yur good to go.
I could be overly paranoid but if see a single pressed grain against the glass I feel like there is at least a small amount of bacteria growing with the myc.  This is also coming from a guy that *really* has a problem with touching door handles and pens. 

I'm not saying this is happening but it seems like you'd have to pc a grain jar for like 6 days to make it absolutely sterile.  This is also why I've been trying to utilize ways to colonize spawn jars in the fastest way possible, to limit the amount of bacteria that can grow.

Nam sayin?


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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #24064663 - 02/04/17 09:22 AM (6 years, 11 months ago)

Hey kenetic, LI takes care of that.  This jar is 13 days old and is already forming multiple stones.  Way ahead of the first round of wedge jars. 



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24067923 - 02/05/17 03:58 PM (6 years, 11 months ago)

Pretty cool progression of the original CN stone whose secondary culture was used for the LI used on this round of rye jars.  I'll just keep transferring it and using the culture it produces for fresh LI as needed, maybe.

Sec. 3:


12/10:


1/22:


Today:


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24067931 - 02/05/17 04:00 PM (6 years, 11 months ago)

:awesomenod::thumbup:


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #24075043 - 02/08/17 01:42 PM (6 years, 11 months ago)

This tamp stone is by far the largest formed so far.  This jar was inoculated on 1/22 from a stone isolate culture.  All around, stone development in these jars noc'd with cultures grown off stones is way ahead of the initial set of jars noc'd with clean ms cultures.  Also, this round is rye where the first set were wheat but I still think the "isolation" is primarily responsible. 



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24075424 - 02/08/17 04:33 PM (6 years, 11 months ago)

Wow, very cool grows going on man!


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Re: Chicon Nindo and tampanensis [Re: Changa Alchemist]
    #24075429 - 02/08/17 04:37 PM (6 years, 11 months ago)

Thanks, Shomann!  I'm getting ready to fruit a couple jars of CN and tamp that aren't really producing stones.  I'm thinking of just casing the grain with a 50/50 jiffy starter/sand mix.  Did you just fruit cased-grain on those last pics of those fat-trunked bitches or did they have a sub?. Shit, I remember now that you answered that already.   


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24075616 - 02/08/17 05:57 PM (6 years, 11 months ago)

Do it. Im very interested to see if yours do the same as mine. Fruited at spawn. 7 flushes.

I also added about 2 cups of coir to my total mix. Just small amounts to add to my grain.

Note: I had to Pasteurize more casing after sever flushes. They eat it.


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Edited by Shomann (02/08/17 06:02 PM)


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Re: Chicon Nindo and tampanensis [Re: Changa Alchemist]
    #24075808 - 02/08/17 07:19 PM (6 years, 11 months ago)

Holla if you print any fruits


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Re: Chicon Nindo and tampanensis [Re: Brain Fart] * 2
    #24080820 - 02/10/17 08:55 PM (6 years, 11 months ago)

GTK about the casing getting eaten, Shomann!  Will do Brain Fart!  Couple close ups of a network feeding up to a new stone forming.  I love this thing!



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24081480 - 02/11/17 06:03 AM (6 years, 11 months ago)

I love the way tamps look when they grow normal shrooms!  Nice pics btw, looks really good on my new  4k 50" tv lol


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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #24081598 - 02/11/17 07:25 AM (6 years, 11 months ago)

What camera are you using?


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Re: Chicon Nindo and tampanensis [Re: Changa Alchemist]
    #24081604 - 02/11/17 07:29 AM (6 years, 11 months ago)

I have been very interested in stones but then I read somewhere that stones have only 65% psilocybin compared to actual fruits. Is that correct? Because I also read somewhere that stones are generally mature to pick after 3 months, but if you leave them longer the potency increases, even leaving them in the jars up to a year. This was supposed to be reputable source for information. Can anyone confirm what I read?


Edited by Cagney (02/11/17 07:30 AM)


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Re: Chicon Nindo and tampanensis [Re: Cagney]
    #24081687 - 02/11/17 08:29 AM (6 years, 11 months ago)

I pulled out the Nikon D3100 and used a cheap macro lense. It takes great pictures but I'm pretty poor at using it.  I'll try for brighter pics next time. 

Not sure on the correlation between age and potency but would prolly harvest anywhere between 3-6 months depending on size. My guess based on ignorance, is that potency is more related to size/mass than age but I could be way off.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado] * 1
    #24081822 - 02/11/17 09:40 AM (6 years, 11 months ago)



I hope you don't mind, I took the liberty of brightening one of the pics in post!


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Re: Chicon Nindo and tampanensis [Re: Aneres]
    #24082174 - 02/11/17 12:53 PM (6 years, 11 months ago)
















My stone grow. They are lots of fun. Awesome to dose on.

I wish I could get my hand on another print lol.


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Re: Chicon Nindo and tampanensis [Re: Brain Fart]
    #24082697 - 02/11/17 05:01 PM (6 years, 11 months ago)

Thanks, Aneres!  That's awesome BF; I got back from the store and saw your post...great minds!  I'm not drilling mine though, just gonna crack the lids and will prolly toss them in a mini mono I have once they get going.  These 2 jars just weren't stoning up, the tamp in particular.  Each container is a 2.5 qt. Steralite and received a qt. of spawn and a cup of BT CVG.  Both were cased with a mix of jiffy starter and sand treated in the same way as the CVG.



Casing mix before hydrating:


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24082791 - 02/11/17 05:46 PM (6 years, 11 months ago)

I grew my atl#7 on straight white millet and just cased with pastuerized jiffy mix seed starter.

The tubs I had drilled already from a previous msg hybrid experiment.

They will grow stones in the substrate too so once the fruits stop flushing tear up the sub and extract stones. The stones get pretty big if left to dry out/stress so towards the end of your flushes keep that in mind.

I just cased my grains didn't spawn to bulk but I also harvested stones before casing. If you just spawn stones and all to bulk should be fine :smile:

Good luck and remember I want a print lol.

Also fruits need to over mature a bit more for stone producing species to give prints IME


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Re: Chicon Nindo and tampanensis [Re: Brain Fart]
    #24082811 - 02/11/17 05:53 PM (6 years, 11 months ago)

Thanks for the insight!  Print with your name on it on my list.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24082881 - 02/11/17 06:16 PM (6 years, 11 months ago)

:bump2::hug::bump2:


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Re: Chicon Nindo and tampanensis [Re: Brain Fart] * 1
    #24087124 - 02/13/17 11:57 AM (6 years, 11 months ago)

Couple jar updates. The first is the tamp stone...definitely putting on some mass.  5 days between these shots:



This is the CN jar that received the majority of the LI but not agar chunks.  The entire jar is filled with stones...hopefully they accrete into a single mass:



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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado] * 1
    #24101210 - 02/18/17 07:54 PM (6 years, 11 months ago)

Just popped these in the mini mono after a week of colonization.  Mycelium is punching through the casing in a couple places.  Anyone suggest patching on these?



I made a couple more and used a sandier casing...you can kinda tell from the pic?  These 2 also had more stones than the above boxes. 



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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24101229 - 02/18/17 07:58 PM (6 years, 11 months ago)

Man it's looking awesome!  A lot can happen in a week lol


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Kenetic]
    #24101240 - 02/18/17 08:02 PM (6 years, 11 months ago)

Fucking finally. Ready to see those babies pin up. Such pretty fruits.


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Changa Alchemist] * 1
    #24106392 - 02/20/17 07:16 PM (6 years, 11 months ago)

Ha!  I'm never in a hurry so shit kinda takes its time on my end.  I patched a bit yesterday but as can be seen the tamp (bottom) is chomping right through the casing and the CN is close to doing the same, so just gonna say fuck it and let it go. I figured as the casing was a jiffy/sand mix and the grains had a cup of CVG to colonize that it would be relatively unscathed...oh well. 



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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24107005 - 02/20/17 11:23 PM (6 years, 11 months ago)

Quote:

Aneres said:


Woah! Neglected tampanesis on agar decided to fruit




that plate is a cutting from a stone that grew on a previous plate. Bah, I should really learn what kinda sub to put em in, these plates have been in good shape all this time


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Aneres]
    #24107459 - 02/21/17 07:25 AM (6 years, 11 months ago)

Hey Aneres, Nice!  Put them to some grain for starters at least.  Bw86 has an awesome grow where he used varied sub of CVG, poo, paper and maybe straw and sand.  It blew the fuck up.  I'm gonna play around with something similar.


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24107560 - 02/21/17 08:45 AM (6 years, 11 months ago)

CVG is what? Constant Velocity Grain lol


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: PlantManBee]
    #24107603 - 02/21/17 09:04 AM (6 years, 11 months ago)

Coir Verm Gypsum.

Constantly Volatile Genitalia


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Brain Fart]
    #24107754 - 02/21/17 10:07 AM (6 years, 11 months ago)

Those babies should be poppin up any day now!


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Changa Alchemist]
    #24107758 - 02/21/17 10:09 AM (6 years, 11 months ago)

Cool thread, looking forward to seeing the fruits popup!


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: lovelaughlibs] * 1
    #24116103 - 02/24/17 12:44 PM (6 years, 10 months ago)

Here's a second box of tamps I just checked on...cased 5 days ago and left to recover...this casing was prolly 80% sand and is almost completely run through....I'll toss any future cased tamp grain boxes directly into fruiting.  The CN boxes on the other hand aren't nearly as aggressive and are not consuming the casing this way.



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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24116330 - 02/24/17 02:37 PM (6 years, 10 months ago)

Can't wait to see some fruits!


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Kenetic]
    #24116993 - 02/24/17 06:54 PM (6 years, 10 months ago)

:standingby:


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: wtfcrazymofo]
    #24128591 - 03/01/17 01:14 PM (6 years, 10 months ago)

First sign of pins!  Ironically, these are popping off the second box of tamps with a predominately sand casing put into fruiting 5 days ago...the first set of boxes have yet to respond and have been in fruiting for 9 days.  In both cases the tamp myc has completely colonized the casing. 



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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24128913 - 03/01/17 02:49 PM (6 years, 10 months ago)

:yess:


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Kenetic] * 1
    #24133220 - 03/03/17 09:38 AM (6 years, 10 months ago)

Slow going but going.  I don't like the "overlay" and it's been tough to stay on top of keeping the surface nice and hydrated. I'm looking forward to fruiting a rye jar with tons of stones in it (these are wheat berries and had no stones).



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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24142003 - 03/06/17 06:55 PM (6 years, 10 months ago)

I think these are going to be small even for tamp standards.  Maybe it'll pick up.



So I dumped this CN jar grown from a stone culture into a small box and cased with jiffy/verm.  These rye jars have done light years better than the wheat jars and will hopefully fruit readily.



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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24143610 - 03/07/17 11:46 AM (6 years, 10 months ago)

Awesome thread Mycolorado!

I am quite fascinated by the Mexicanae, not for stones though, but haven't gotten to them yet.

I wonder what your reasoning behind using so much sand in your casing is? (Don't mean to sound rude here bro)
And also what are your substrate depth on CN?

I'm gonna try Jalisco grown in bottles but are a bit wary about the subs. depth. Might just end up crumbling them into lower trays if nothing happens lol.

Good luck with your cult adventure!


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: knorr]
    #24143702 - 03/07/17 12:18 PM (6 years, 10 months ago)

Thanks, knorr!  User bw86 used sand to great success when fruiting his stone producers (whether it had any impact, I don't know).  Also, from what I've gathered, tamps were initially found growing in a very sandy environment.  On top of that, I often find morels growing in sandy soil, which also produce sclerotia, so I figured I'd give it a try.  They're about 3 inches deep but might do better with more surface area and less depth...like 2 inches.  In the first boxes I used a cup of cvg to qt of spawn just to add some moisture.  This last box is just spawn.


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24145560 - 03/08/17 05:54 AM (6 years, 10 months ago)

Yeah sand seems to be a good ingredient for the Mexicanas for some reason.

Surface area doesn't seem to be all that important with cubes and CaptainFuture had very nice results with ms in a mycobag (it was hard to estimate substrate depth from his pictures but it seemed to be around 4") so I feel positive about the bottles working!


I've only read about sand being used under the substrate, with holes in the FC, and in the casing layer which makes me wondering if it could be useful inside the sub as well.


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: knorr] * 1
    #24148355 - 03/09/17 09:13 AM (6 years, 10 months ago)

You're prolly right on depth.  I'm gonna sterilize some bags with a varied "kitchen sink" sub...coir, verm, paper, poo, straw, etc. and see how it fares that way. 

I'll give these tamps a couple more days and re-case with jiffy/verm.



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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado] * 1
    #24149509 - 03/09/17 06:03 PM (6 years, 10 months ago)

:splooge:

Finally.

This thread is solid myco. Very good source info pouring from all ends!


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Changa Alchemist]
    #24149810 - 03/09/17 08:22 PM (6 years, 10 months ago)

Im hoping to get some CN prints soon, this thread is very informative. Thanks


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: PaulWall]
    #24149948 - 03/09/17 09:27 PM (6 years, 10 months ago)

How are those cased CN myco? I've got a good feeling. How old was the spawn you used from a stone culture?


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Changa Alchemist]
    #24150035 - 03/09/17 10:01 PM (6 years, 10 months ago)

:highfive1:

Damn myco, killing it dude! Stoked to come back and see this.


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Marty Mycfly]
    #24150075 - 03/09/17 10:19 PM (6 years, 10 months ago)

The CN spawn was 6 weeks old.  I'm excited to see how they do...tomorrow evening is day 4 so hopefully they'll be coming along soon thereafter.  The stones and all around better appearance/prep of the spawn compared to the wheat spawn I'm current fruiting is encouraging.  I also noticed a couple pins in the initial tamp box that's been in fruiting for 19 days. Thanks all for following along and participating in this thread!
Quote:

Marty Mycfly said:
:highfive1:

Damn myco, killing it dude! Stoked to come back and see this.




Hey Marty; welcome back!  Be good to see you on the boards again!


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24159125 - 03/13/17 03:41 PM (6 years, 10 months ago)

Day 13 since pins...pretty slow moving.  I just cracked the lids of the monos they're in to give them some more FAE.  They do keep coming up though, which is encouraging.



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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24159343 - 03/13/17 04:54 PM (6 years, 10 months ago)

Seems like they're holding out on you


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Kenetic]
    #24161993 - 03/14/17 03:33 PM (6 years, 10 months ago)

Those are some gorgous mushrooms, I'm not familiar with fruiting times with these, what is a normal flush usually?


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Marty Mycfly]
    #24162055 - 03/14/17 03:52 PM (6 years, 10 months ago)

From what I'm gathering, they're pretty temp dependent when it comes to fruiting initiation and duration....if temps are correct (sounds like 75-80F), they finish in a few days.  Unfortunately, my temps are pretty low in the grow area ATM and they're not really doing much...shomann and kalsticky have both had real nice flushes and fruits as well.


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24162073 - 03/14/17 03:58 PM (6 years, 10 months ago)

I'm going to be spawning 12 quarts of tampanensis this weekend. I'll do some with subs, some with cased grains. 6 are from one petri dish and 6 are from another.. I'll try both of them both ways and see what happens.

My temp range is right in that area. 69-78° give or take 2°.

How are the CN myco?

Edit: I think I used your post to answer comments in the stone producer thread too. My bad. :rofl:


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Edited by Shomann (03/14/17 03:59 PM)


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Changa Alchemist]
    #24162088 - 03/14/17 04:02 PM (6 years, 10 months ago)

Nice...keep us posted on that.  The CN just started poking up through the casing, so hopefully we'll get some fruit this week.  If not, I'm gonna move them to a warmer area.


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Re: Chicon Nindo and tampanensis (stones and fruit) [Re: Mycolorado]
    #24163835 - 03/15/17 09:13 AM (6 years, 10 months ago)

Really wish these would take off.  On a positive note, the first box is starting to push up some fat little pins with deep color.



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