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InvisibleMycolorado
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Chicon Nindo and tampanensis (stones and fruit)
    #23892271 - 12/03/16 10:07 PM (7 years, 1 month ago)

Guess I'll throw out some pics as well.  Here's some CN and tamp cultures I'm working on.


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OfflineFerather
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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23892939 - 12/04/16 05:41 AM (7 years, 1 month ago)

Are the brown ones black tea? They look identical in speed, the TA looks more populated (less gaps) on the brown ones.


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Re: Chicon Nindo and tampanensis [Re: Ferather]
    #23893063 - 12/04/16 07:50 AM (7 years, 1 month ago)

Maybe it's coffee because coffee soaked grains seem to improve the production of sclerotia. Never tried coffee dishes. Stones do not develop on my plates for some reason... After inoculation stones start to to develop in the jars but even on very old dishes I don't have stones.

Maybe I should try to add coffee to the agar.
I don't know if it's coffee or tea but anyway..
:threadmonitor:


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OfflineFerather
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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23893088 - 12/04/16 07:59 AM (7 years, 1 month ago)

Nvm, its probs coffee, the first set says "cafe" at the bottom.

:takingnotes:


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23893113 - 12/04/16 08:14 AM (7 years, 1 month ago)

Yep, coffee (weak coffee MEA).  I made them up as it was mentioned on here that it promotes stones.  They are moving a tad slower than the straight MEA plates...my guess is due to more nutes in the coffee.  Unfortunately, the phone camera doesn't come close to doing these justice...they're beautiful!  The tamps developed from a single colony and the CN plate had 3...a couple are producing stones.  Colony 3 produced a stone right off the bat...I'll transfer it soon.  I might try some plates with silica added to see if that has any effect on stone production. Here's the stone plate.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23893122 - 12/04/16 08:18 AM (7 years, 1 month ago)

Quote:

my guess is due to more nutes in the coffee




Too much coffee slows the mycelium growth. I noticed this by soaking rye in too strong coffee. Coffee is acidic that might be the reason for the slow growth if you use too much.


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23893159 - 12/04/16 08:39 AM (7 years, 1 month ago)

GTK.  I diluted it to decrease the nute content but also figured it might have similar inhibitory effects as the tea so didn't want to use strong coffee.  At this point, I'm not seeing any noticeable difference with the exception of speed which is minor.  I read that sand is beneficial in or used solely as the casing when it comes to fruiting and was curious if silica will have any effect on stone production as well. Gonna test some silica plates soon and will share those results. Morels produce sclerotia and I often find those in sandy soil...


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23893172 - 12/04/16 08:46 AM (7 years, 1 month ago)

Quote:

enlightenment said:
Quote:

my guess is due to more nutes in the coffee




Too much coffee slows the mycelium growth. I noticed this by soaking rye in too strong coffee. Coffee is acidic that might be the reason for the slow growth if you use too much.



Same issue with lots of tea, too acidic, if you want more add lime to balance.


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Offlinewtfcrazymofo
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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23893185 - 12/04/16 08:50 AM (7 years, 1 month ago)

:takingnotes::popcorn::thumbup:


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Re: Chicon Nindo and tampanensis [Re: wtfcrazymofo]
    #23893245 - 12/04/16 09:24 AM (7 years, 1 month ago)

Catsan, or similar lime products, notice calcium carbonate (lime) is used for antibacterial reasons.
Anyway point being it can be used as grit, or direct casing maybe, don't know.

Side note, limed tea (Ph 6) is a bit of a bacteria bomb.


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InvisibleMycolorado
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Re: Chicon Nindo and tampanensis [Re: Ferather]
    #23899445 - 12/06/16 10:06 AM (7 years, 1 month ago)

The donor tamp plate is starting to form stones at 1, 6 and 8 o'clock.  Pretty rapid development as I didn't notice these yesterday.  Also looks like some pins might be forming around the perimeter of initial growth. 


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23913314 - 12/10/16 02:32 PM (7 years, 1 month ago)

Transferred some stones from the original tamp and CN plates.  This was the larger of the 2 CN stones transferred...it was dense!

The tamp stone carried a little more mycelium than I cared for but oh well.

Here's the nicest looking tamp transfer starting to form a stone.

A good looking variety of cultures going to grain


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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23915877 - 12/11/16 10:21 AM (7 years, 1 month ago)

Quote:

enlightenment said:
Stones do not develop on my plates for some reason...:



I was just looking over TMC and it says stones form better in total darkness.  My plates are forming stones quite readily and are kept in the dark.  It also states that stone production on plates was highest at 4.5% MEA.  Wanted to throw that out in case you hadn't seen it.


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stonesun
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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23915891 - 12/11/16 10:27 AM (7 years, 1 month ago)

TY. Today I cloned some Psi galindoi stones to agar.



I used 10g LME for 500ml water.
That's my standard agar recipe. I use it with everything. Sometimes I make a batch of PDA dishes.

Let's see how the dishes grow.


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InvisibleMycolorado
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Re: Chicon Nindo and tampanensis [Re: enlightenment]
    #23950000 - 12/22/16 07:49 PM (7 years, 1 month ago)

Stone plates are looking good. The smaller of the CN stones has put out nice, quick, uniform growth but unfortunately has overgrown a small bac colony.  The bigger one hasn't grown out as much but looks like it may be startting a new stone.

The original tamp plate keeps developing stones...will continue to harvest and start new plates till it dries up.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23957743 - 12/26/16 06:47 AM (7 years, 1 month ago)

Quote:

Mycolorado said:
Quote:

enlightenment said:
Stones do not develop on my plates for some reason...:



I was just looking over TMC and it says stones form better in total darkness.  My plates are forming stones quite readily and are kept in the dark.  It also states that stone production on plates was highest at 4.5% MEA.  Wanted to throw that out in case you hadn't seen it.





Merry Xmas Myc

If I was going to use PADY would you try 4.5 % with this?  I dont have any Malt atm


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InvisibleMycolorado
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Re: Chicon Nindo and tampanensis [Re: Edmunter]
    #23957849 - 12/26/16 08:39 AM (7 years, 1 month ago)

I'd say give it a try, though I really couldn't speculate as I've only run them on 3% MEA...I'm gonna try them out on DFA, prolly 3% as well, to see how they do on it.  I haven't gone up to 4.5% yet as the cultures I'm working with are producing stones fine as is...may just be good genetics, which is great cause these prints came from an awesome shroomerite.  Here's a tamp jar just getting going and would appear stones are already beginning to develop.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23960463 - 12/27/16 12:42 PM (7 years, 1 month ago)

Ive taken 1 pin from this plate already, im going to take another but do you see any stones or areas that look decent for transfer for stones?



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Re: Chicon Nindo and tampanensis [Re: Edmunter]
    #23960569 - 12/27/16 01:37 PM (7 years, 1 month ago)

Looks like some stones forming in a couple of those pics unless they're knots.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23960574 - 12/27/16 01:39 PM (7 years, 1 month ago)

Its one plate.  Are the small white knot like areas stones you think.  There are browning areas too.  Im transferring tomorrow so be good to get it right


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