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The_breadsticks
Stranger

Registered: 09/23/16
Posts: 220
Last seen: 3 years, 5 days
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Agar question and contam question
#23891804 - 12/03/16 07:41 PM (7 years, 1 month ago) |
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Hey guys, is this agar looking ok? As in does it look like cubensis?!
Also, what is contaminating these jars? One was fine until I shook it. The other is a g2g transfer from 2 days ago. I thought the jar was clear... Help?!

Also, what is this yellow stuff on my grains?! Right in the middle towards the top.. it's a fuzzy pale yellow. Everything is contaming!!
-------------------- FREE TUMP
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Sorry, everything I see is fucked
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 8 hours, 18 minutes
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agar?
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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The_breadsticks
Stranger

Registered: 09/23/16
Posts: 220
Last seen: 3 years, 5 days
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Re: Agar question and contam question [Re: wtfcrazymofo]
#23892307 - 12/03/16 10:22 PM (7 years, 1 month ago) |
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Quote:
wtfcrazymofo said: agar?
Crap, the age pic didn't go. Yes all that is fucked, but what's fucking it? Heres the agar.

I really need a new phone. These pics suck.
-------------------- FREE TUMP
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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that looks good
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 8 hours, 18 minutes
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Quote:
The_breadsticks said:
Quote:
wtfcrazymofo said: agar?
Crap, the age pic didn't go. Yes all that is fucked, but what's fucking it? Heres the agar.

I really need a new phone. These pics suck.
Hopefully you went ahead and took some transfers from this plate so you could try again. Did you pc your jars long enough? Are you brushing up on your sab skills? Is you moisture content to heavy?
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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The_breadsticks
Stranger

Registered: 09/23/16
Posts: 220
Last seen: 3 years, 5 days
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Re: Agar question and contam question [Re: wtfcrazymofo]
#23892910 - 12/04/16 05:13 AM (7 years, 1 month ago) |
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Quote:
bodhisatta said: that looks good
Perfect, thanks. I can't seem to get clean spawn, so I think im going to try a tiger drop or two.Quote:
wtfcrazymofo said:
Quote:
The_breadsticks said:
Quote:
wtfcrazymofo said: agar?
Crap, the age pic didn't go. Yes all that is fucked, but what's fucking it? Heres the agar.

I really need a new phone. These pics suck.
Hopefully you went ahead and took some transfers from this plate so you could try again. Did you pc your jars long enough? Are you brushing up on your sab skills? Is you moisture content to heavy?
I didn't take transfers but I have about 30 plates of that same culture.
I've been using a shmuvbox. Should I go back to my sab? Moisture content is a 24 or less hour soak and then dried on the surface.
 This look ok?
-------------------- FREE TUMP
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 8 hours, 18 minutes
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Are these plates ms drop ones? Did you transfer away from the pile of spores? Just get a flowhood or go back to the sab. That jar from the pic is hardly ok. It looks bacterial, but hard to tell.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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The_breadsticks
Stranger

Registered: 09/23/16
Posts: 220
Last seen: 3 years, 5 days
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Re: Agar question and contam question [Re: wtfcrazymofo]
#23893130 - 12/04/16 08:23 AM (7 years, 1 month ago) |
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These are on their 3rd transfer after ms I believe.
I also have a few jars I inoculated ms and I've taken a few pieces of colonized grain and dropped em on plates as well.
As soon as my favorite vendor puts their Christmas special up, I'll be getting more spore so I can start fresh plates. I just started 8 plates from a panaeolus cambodginiensis print, kinda excited.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 8 hours, 18 minutes
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How long did you pc jars? You ought to try foo mans wbs prep tek.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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The_breadsticks
Stranger

Registered: 09/23/16
Posts: 220
Last seen: 3 years, 5 days
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Re: Agar question and contam question [Re: wtfcrazymofo]
#23893619 - 12/04/16 11:58 AM (7 years, 1 month ago) |
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I'm using oats, and inocules oat prep. I normally PC 90 mins to 2 hours.
Are these ok? One is g2g, the other ms. All 3 ms jars look like this. And 6 of 9 of the g2g look like thi$. It definitely isn't the nasty contams of other jars, and they smell fine.

Will someone please just send me a clean master jar? Lol
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Edited by The_breadsticks (12/04/16 11:59 AM)
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
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The picture quality is bad but the jars look OK from what I can see.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Agar question and contam question [Re: enlightenment]
#23893684 - 12/04/16 12:17 PM (7 years, 1 month ago) |
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I don't see any GE modifications to any of those lids, are there any?
Edit: Nvm I see them, last image.
Edited by Ferather (12/04/16 12:25 PM)
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The_breadsticks
Stranger

Registered: 09/23/16
Posts: 220
Last seen: 3 years, 5 days
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Re: Agar question and contam question [Re: Ferather]
#23893785 - 12/04/16 12:42 PM (7 years, 1 month ago) |
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Quote:
Ferather said: I don't see any GE modifications to any of those lids, are there any?
Edit: Nvm I see them, last image.
Yup I'm using easy felt. The jars that I leave alone after the PC cycle have sat for weeks with no contams. It has to be my spawn or sterile tech. Maybe this old apartment is moldy as fuck.
I'll try to get some better pics up today! Thanks guys.
Btw, I'll be picking up some sfd to make new lids in a few days. Is staments site the best place?
-------------------- FREE TUMP
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The_breadsticks
Stranger

Registered: 09/23/16
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Last seen: 3 years, 5 days
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One is ms one is g2g. Are either OK?
Recently did a few via tiger drop. Should def get a clean jar from at least one of those.

They smell correct
-------------------- FREE TUMP
Edited by The_breadsticks (12/05/16 03:59 PM)
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
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Those jars look bacterial to me. You can spawn them anyway but they won't perform as good as clean grain jars + they make the substrate more vulnerable to other contaminations like mold.
It is your decision if you want to spawn them or not. I would spawn jars like these because they are not too messed up and can produce one or two nice flushes sometimes. Jars with slimy spots or a lot of uncolonized parts that look wet should not be spawned.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 8 hours, 18 minutes
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Oats tend to go bacterial if prepped to wet try eats oat prep (alot less babysitting and no fanning)https://www.shroomery.org/forums/showflat.php/Number/22158748 I use this tek #1 with great success running Li's and agar wedges. I even got a bag of producers pride (infamously bacterial) that I PC Quart jars for 2 hours.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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The_breadsticks
Stranger

Registered: 09/23/16
Posts: 220
Last seen: 3 years, 5 days
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Re: Agar question and contam question [Re: wtfcrazymofo]
#23903389 - 12/07/16 01:30 PM (7 years, 1 month ago) |
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K I've got some jars I prepped that way that I will be noccing tonight. Thanks for the recommendation!!
Do you find that the oats are fine with no rinse? Ive had grain go sticky because it took to long to dry!
And also, gypsum?
My first mono tub is pinning pretty hard, I'm very excited! I did 1 run of pftek with 20 cakes. It worked but getting the cakes to pin was kinda a pain in my butt. Now im going to do some brf cakes but only for slurry.
I wonder if you could make a half pint master with some sort of grain and just steaming..? I'll invent my own beginner tek. Call it BStek
-------------------- FREE TUMP
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
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Quote:
What kind of damage does shaking cause? Did I shoot myself in the foot?
unless it is rice you wont have much success with steaming grain. BTW: BS-tek would be the right label for a steaming grain TEK 
*jk
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The_breadsticks
Stranger

Registered: 09/23/16
Posts: 220
Last seen: 3 years, 5 days
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Re: Agar question and contam question [Re: enlightenment]
#23903743 - 12/07/16 02:46 PM (7 years, 1 month ago) |
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Quote:
enlightenment said:
Quote:
What kind of damage does shaking cause? Did I shoot myself in the foot?
unless it is rice you wont have much success with steaming grain. BTW: BS-tek would be the right label for a steaming grain TEK 
*jk
I know it, the name works on 2 levels! Breadstick/BULLSHIT! Hahah
So I've currently got some half pints nocced with LI. From there, would my best bet be go g2g? I've also got about 20 qt jars that I hit with the LI... Is there any advantage to doing the small masters other than colonization time?
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