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OfflineShu
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Registered: 11/20/11
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Is my spawn contaminated?
    #23891774 - 12/03/16 07:32 PM (7 years, 1 month ago)



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Invisiblevatman
I'm Vatman


Registered: 04/17/14
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Re: Is my spawn contaminated? [Re: Shu]
    #23891783 - 12/03/16 07:36 PM (7 years, 1 month ago)

Is that yellows growth? Looks really bad.


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OfflineShu
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Re: Is my spawn contaminated? [Re: vatman]
    #23891802 - 12/03/16 07:41 PM (7 years, 1 month ago)

It's growth but i'm not sure what. I put a drop of spore suspension in each of 13 jars, 2 went wonky.


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Invisiblevatman
I'm Vatman


Registered: 04/17/14
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Re: Is my spawn contaminated? [Re: Shu]
    #23891836 - 12/03/16 07:51 PM (7 years, 1 month ago)

Center one looks fine, The right one looks like trash and left one looks the yellow growth is starting show on that.

did you use a still air box?


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OfflinePinPornProducer
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Re: Is my spawn contaminated? [Re: vatman]
    #23891862 - 12/03/16 07:58 PM (7 years, 1 month ago)

Middle might be good, toss the other 2 yesterday


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OfflineShu
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Re: Is my spawn contaminated? [Re: vatman]
    #23891863 - 12/03/16 07:58 PM (7 years, 1 month ago)

Quote:

vatman said:
Center one looks fine, The right one looks like trash and left one looks the yellow growth is starting show on that.

did you use a still air box?



Yes, I use this glove box.


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OfflineXero1
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Re: Is my spawn contaminated? [Re: Shu]
    #23891914 - 12/03/16 08:18 PM (7 years, 1 month ago)

That right one looks like you squirted mustard in there lol. Yea man deff toss


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Invisiblevatman
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Re: Is my spawn contaminated? [Re: Xero1]
    #23891954 - 12/03/16 08:31 PM (7 years, 1 month ago)

Looks like an old school glove box you got going there.

Was it spores or agar to grain?
Looks a little dirty in there. Was wondering the rate of dish contamination


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OfflineShu
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Re: Is my spawn contaminated? [Re: vatman]
    #23892145 - 12/03/16 09:28 PM (7 years, 1 month ago)

Quote:

vatman said:
Looks like an old school glove box you got going there.

Was it spores or agar to grain?
Looks a little dirty in there. Was wondering the rate of dish contamination



That pick was taken at the end of a cloning session so it's a bit messy. The iodine and sulfur stains are from its first home in a moisture testing lab. When working with clean cultures I get about one mold hit every three sleeves. I never see bacteria or yeast on my plates but I did get one yeast hit in a batch of 30 slants recently but nothing else. This KSSS syringe it contaminating 1 in 5 at 1 drop per jar.


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InvisibleMad Season
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Re: Is my spawn contaminated? [Re: Shu]
    #23892507 - 12/04/16 12:13 AM (7 years, 1 month ago)

Quote:

Shu said:
It's growth but i'm not sure what. I put a drop of spore suspension in each of 13 jars, 2 went wonky.




Left is aspergillus, middle is thick af. Could be a sign of some bacteria present. Not really much to worry about tho. Right is a slime mold. Both slime mold and aspergillus die in the PC so you have to have introduced them after the PC.


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