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Shrms
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Beginner starts edibles (Pleurotus ostreatus/eryngii)
#23889765 - 12/03/16 06:45 AM (7 years, 1 month ago) |
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Hello together,
after growing cubensis and panaeolus I am going to try growing some edibles, starting with Pleurotus ostreatus/eryngii and shiitake. I am absolutely new to cultivation of these species, but i am looking forward to it.
Here what i have done until now:
Few weeks ago I bought fresh oyster and eryngii mushrooms from our supermarket and cloned it (cut a piece out of the middle of the stem and throwed it on agar). The mycelium colonized the agar very fast, faster than any cubensis / panaeolus I have seen before. After that I prepared rye (RR's procedure), in the same way I prepare for all mushrooms before.
The mycelium colonizes fast as hell, but it looks a little strange to my. Very thin and long strings, comparable to cobweb maybe (know cobweb from photos, never saw it real). But ok, it is another species and not comparable to the mycelium i am used to.
On day six after inoculation or so, some white knots developed in both oyster jars (jar was 50% colonized i think).
Today, 11 days after inoculation there are thousands of white knots everywhere, jar is nearly at 100% colonization.
The eryngii jars are at 95% colonization and don't show the white stuff.
Here are some photos:
Oyster jars:

Eryngii:

Here are my questions: 1.Does this look normal so far? Escpecially the oyster jars. 2.Should I shake the jars for faster colonization? Maybe to late now
My plan is to use a straw based substrate and growing in bags, but i don't have a detailed strategy yet... have to do some reading work in the next days
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Ferather
Mycological



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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Shrms]
#23889791 - 12/03/16 07:08 AM (7 years, 1 month ago) |
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At first, the first image said growing from condensate, does happen. The rest say bacterial avoidance. The glass is acting as a safe grow zone away from bacteria, where it can grow freely.
Take note of the patching, and the surface says "escape" all over.
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Shrms
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Ferather]
#23889844 - 12/03/16 07:44 AM (7 years, 1 month ago) |
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I am not sure if I get you right, you say all of the jars are bacterial and the mycelium is trying to escape 
That's bad news, so I have to dump everything and start from scratch or is there a chance that the mycelium wins?
I did a lot of other rye jars (cubensis) and they are doing well.
Another question, what temperature would you recommend for these species? They growed at room temperature (65°) and I did not put them into my incubator (82°).
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Ferather
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Shrms]
#23889895 - 12/03/16 08:14 AM (7 years, 1 month ago) |
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The Oyster I was talking about, the Eryngii looks ok so far.
What would you spawn to if you left it to continue?
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Quadman
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Shrms]
#23889903 - 12/03/16 08:21 AM (7 years, 1 month ago) |
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Room temp is fine for oysters. Considering you never shook there could be some areas like you have. I don't see metabolites. If it were me I would wait a bit longer. If They fill in some more use them. Shake the kings , the bottom pictures?
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Shrms
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Ferather]
#23889928 - 12/03/16 08:37 AM (7 years, 1 month ago) |
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Quote:
Ferather said: The Oyster I was talking about, the Eryngii looks ok so far.
What would you spawn to if you left it to continue?
My plan was to use pasteurized straw as substrate (in a bag), maybe I can add some wood chips. I am looking for a detailed recipe currently, but these are the materials I have at home right now.
Maybe an alternative are straw pellets, as I learned today it is not necessary to pasteurize the substrate. Sounds easy and time saving, but I don't have them right now.
Quote:
Quadman said: Room temp is fine for oysters. Considering you never shook there could be some areas like you have. I don't see metabolites. If it were me I would wait a bit longer. If They fill in some more use them. Shake the kings , the bottom pictures?
I did not shake any of the jars because I did not know if it is possible and good for these strains.
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Mattisfat
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Quadman]
#23889930 - 12/03/16 08:37 AM (7 years, 1 month ago) |
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I have a feeling the oyster jars are going to turn green soon
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MorePies
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Mattisfat]
#23889985 - 12/03/16 09:15 AM (7 years, 1 month ago) |
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With pluerotus, I find that after dropping the wedges in a d waiting a day or two for them to recover before shaking, they are less likely to stick to the sides. This happens to me a fair amount when I shake tight after inoculating. After that, shaking again at 25%-30% is enough.
The top pictures look contammed. If they weren't, the last picture could have been used a day or two ago. I look for complete mycelia coverage, but still being able to see individual grains.
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Shrms
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: MorePies]
#23943114 - 12/20/16 11:20 AM (7 years, 1 month ago) |
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Hey,
I gave it a try and used all jars and spawned them to substrate consisting of straw pellets, gyspum, lime according to the TEK of AlCapone2k.
I created 2 bags (2 jars per bag) about 12 days ago. I saw some white spots of mycelium after one day and after 2 or 3 days white spots everywhere.
But the last 10 days nothing happens, it seems mycelium is sleeping, at least at the surface.
Here some pics (left = eryngii ; right = oyster)

So what do you think, are they both contaminated or are there other reasons? Maybe to little holes (which I poked through the bag with a small needle) and as a consequence not enough air exchange?
I cannot detect any weird spots that look like contaminations, but I don't have the experience to realize it.
Both bags look completely the same to me, so I hope there can be another reason
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Quadman
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Shrms]
#23943318 - 12/20/16 12:38 PM (7 years, 1 month ago) |
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Yes they need more than a few needle holes. My fear is the bags becoming anaerobic. The kings should be quite a bit slower. No sign of contaminte. Looking good!
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Edited by Quadman (12/20/16 02:55 PM)
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Ferather
Mycological



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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Quadman]
#23943374 - 12/20/16 12:57 PM (7 years, 1 month ago) |
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It's actually difficult to suffocate something that can grow into solid wood, underground or underwater. 99.99% chance of bacterial deterioration of the substrate along side pathogenic behavior.
Picture 3 has mild metabolites, but also yellow mycelium (taking damage). I also see decaying straw and no mycelium present in patches.
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Quadman
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Ferather]
#23943542 - 12/20/16 01:51 PM (7 years, 1 month ago) |
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If that were true we could eliminate expensive filter patch bags, holes in jar lids. The problem is going anaerobic without sufficient air exchange.
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Ferather
Mycological



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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Quadman]
#23943619 - 12/20/16 02:14 PM (7 years, 1 month ago) |
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I have never modified any of my containers, although I use clip on lids.
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AlCapone2k
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Ferather]
#23949166 - 12/22/16 01:25 PM (7 years, 1 month ago) |
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Hey mate,
I'm glad that you use my "tek"
Unfortunately it seems that your myc is stalling due to contamination. The jards didn't look that well, actually I already have fruited jars like yours and they worked, but I also had these stalling behaviour with jars like yours. I think it's a bit "rolling a dice".
Actually it should not take more than 5 days until the whole bale looks white.
How many holes have you stabbed into the foil? I stab 4 holes from top to the bottom every 0,5-1 inch around the bale, which means a lot of holes. Maybe you had too less?
I'm sorry that it didn't work for you
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Shrms
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: AlCapone2k]
#23949297 - 12/22/16 02:17 PM (7 years, 1 month ago) |
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I stabbed a lot of holes, I cannot say exactly but maybe too less. Furthermore it was a very thin needle, maybe the holes are to small.
I opened both bags 2 days ago to give a fresh air boost and stabbed more holes with a bigger needle and it seems to work for the eryngii but I a not sure.
Here's a pic from today:

I just wait some more days, but I think the right one (oyster) is trash
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AlCapone2k
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Shrms]
#23949309 - 12/22/16 02:21 PM (7 years, 1 month ago) |
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I also think so, it should be really, really white after these days of colonization.
During colonization you don't need so much FAE, just GE, which is established through the small holes all around the bale.
What temperature do you have in the room the bales are in?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Shrms
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: AlCapone2k]
#23949321 - 12/22/16 02:27 PM (7 years, 1 month ago) |
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It is about 66°F (19° C) give or take, that's the same temperature range during colonization of the rye jars where it worked pretty well.
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AlCapone2k
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Shrms]
#23949364 - 12/22/16 02:42 PM (7 years, 1 month ago) |
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yeah, 19 °C is absolutely fine. This is not the reason.
I think at least for the second bale it's a contamination.
The smalls holes aren't any problem - sometimes I use a normal cloth needle, so the holes can't be too small
Maybe some time will bring a full colonization.
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Forrester
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: AlCapone2k]
#23950110 - 12/22/16 08:32 PM (7 years, 1 month ago) |
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I almost want to totally agree that those first jars of oysters looked contaminated, if it weren't that I've had many, many jars of oysters look just like that with all the white knots, and end up growing out just fine. Yeah it probably is contaminated to some degree, but I'd do what Al said and just give it some time.
Those bags do look quite moist, maybe that's the reason for slow colonization? Either way, I'd still give it time and see what happens, oysters are quite aggressive.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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AlCapone2k
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Re: Beginner starts edibles (Pleurotus ostreatus/eryngii) [Re: Forrester]
#23952008 - 12/23/16 03:49 PM (7 years, 1 month ago) |
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Actually the moisture content seems to be the same like it is in my bales. It has to be quite moist in there.
I think it's just bad spawn which led to this stalling. The jars doesn't look good actually.
I would just let these bales colonize, if they get really white, start fruiting. If they stalled totally, toss them or bury them in your garden.
Start again and you'll do better, don't give up!
Bales have to look similiar like them after at least two weeks:
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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