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Psyche delics
Hypochondriac



Registered: 09/24/15
Posts: 3,644
Last seen: 4 months, 18 days
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How common are contaminated syringes?
#23889240 - 12/02/16 10:33 PM (7 years, 1 month ago) |
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Ive had two succesfull grows with syringes I got from x I ordered from y and and all of my projects have been contaminated I KNOW I did EVERYTHING right these last few times but I still keep getting contamination. I just killed my last syring on some brf cakes and it looks like they got contaminated too unless its early bruised mycelium? And its right underneath the inoculation holes.
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Edited by Psyche delics (12/02/16 10:44 PM)
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Greg
always learning



Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
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Re: How xommon are contaminated syringes? [Re: Psyche delics]
#23889257 - 12/02/16 10:38 PM (7 years, 1 month ago) |
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400 posts and you still haven't read the rules? For shame.
Quote:
3. Spores are sold for microscopy use only so mycologists can ID mushrooms. If you're going to misuse them to grow a controlled substance, keep it to yourself. Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning. Do not plug non sponsors.
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Psyche delics
Hypochondriac



Registered: 09/24/15
Posts: 3,644
Last seen: 4 months, 18 days
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Re: How xommon are contaminated syringes? [Re: Greg]
#23889263 - 12/02/16 10:40 PM (7 years, 1 month ago) |
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Lol i just realized that I did that stupid mistake
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A.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
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Re: How xommon are contaminated syringes? [Re: Psyche delics]
#23889271 - 12/02/16 10:45 PM (7 years, 1 month ago) |
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I ONLY use ***Sponsors***. Most syringes have some contamination from what I know. ***Sponsor**** has run me through many microscopy kits with very low contam rate. I'd check your teks. It seems many new members have great sucsess for a few then contam their clean areas some how. I can not give you reasons, as I am still pretty new to the hobby. I will stand up for a quality vendor every time though. I hope you get it sorted. Dick
Edit: We don't mention spore vendors in mush cult period.
-------------------- "When eating shit, it is best not to nibble. Bite, Chew. Swallow. Repeat." "If you're making love to your old lady, someone else is fucking her" "Douchebags are children who never grew up, like Sheeklette, we should pity them." [quote]Niffla said: [quote]A.RichardTrickle said: Dick[/quote] http://www.youtube.com/v/kbwNUOUy-3c[/quote]
Edited by Pastywhyte (12/03/16 12:10 AM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: How xommon are contaminated syringes? [Re: Psyche delics]
#23889273 - 12/02/16 10:46 PM (7 years, 1 month ago) |
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you are not providing nearly enough details.
how did you inoculate?
pf cakes=MS ... almost all spores will be somewhat dirty.
the 'I KNOW I DID EVERYTHING RIGHT' line means you fucked up.
seriously dick trickle??. read that rule and read your post.
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Psyche delics
Hypochondriac



Registered: 09/24/15
Posts: 3,644
Last seen: 4 months, 18 days
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Re: How xommon are contaminated syringes? [Re: mushboy]
#23889289 - 12/02/16 10:55 PM (7 years, 1 month ago) |
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"I know I did everything right" i posted a video of me knocking up agar on the noob forum they pointed put my possible mistakes and I always read the tek while perfoming it plus ive gotten success other times.
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A.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
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Re: How xommon are contaminated syringes? [Re: mushboy]
#23889296 - 12/02/16 10:58 PM (7 years, 1 month ago) |
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Quote:
mushboy said: you are not providing nearly enough details.
how did you inoculate?
pf cakes=MS ... almost all spores will be somewhat dirty.
the 'I KNOW I DID EVERYTHING RIGHT' line means you fucked up.
seriously dick trickle??. read that rule and read your post.
What? I looked at syringes under scope and found few contams? Are you suggesting I am doing something wrong, or worse illegal? I think YOU need to reread my post friend. Dick
-------------------- "When eating shit, it is best not to nibble. Bite, Chew. Swallow. Repeat." "If you're making love to your old lady, someone else is fucking her" "Douchebags are children who never grew up, like Sheeklette, we should pity them." [quote]Niffla said: [quote]A.RichardTrickle said: Dick[/quote] http://www.youtube.com/v/kbwNUOUy-3c[/quote]
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: How xommon are contaminated syringes? [Re: Psyche delics]
#23889346 - 12/02/16 11:19 PM (7 years, 1 month ago) |
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Quote:
Psyched delics said: "I know I did everything right" i posted a video of me knocking up agar on the noob forum they pointed put my possible mistakes and I always read the tek while perfoming it plus ive gotten success other times.
i watched and read the thread.. mostly about needle flaming but you work is ok.
but what does that have to do with MS into pf jars?
no agar in a MS>pf jar.
how did you inoculate the jars?
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Psyche delics
Hypochondriac



Registered: 09/24/15
Posts: 3,644
Last seen: 4 months, 18 days
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Re: How xommon are contaminated syringes? [Re: mushboy]
#23889385 - 12/02/16 11:33 PM (7 years, 1 month ago) |
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Im sorry but I dont quite understand. are you asking if I used ms if so yes
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
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Re: How xommon are contaminated syringes? [Re: Psyche delics]
#23889391 - 12/02/16 11:37 PM (7 years, 1 month ago) |
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i asked how did you inoculate the jars in question and you answered with something completely unrelated.
did you inoculate inside the SAB or did you do it in a bathroom?
any time you do spores you are gambling with the contam game. spores are like 90% dirty.
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Psyche delics
Hypochondriac



Registered: 09/24/15
Posts: 3,644
Last seen: 4 months, 18 days
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Re: How xommon are contaminated syringes? [Re: mushboy]
#23889439 - 12/03/16 12:13 AM (7 years, 1 month ago) |
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I used a sab im stupid but not that stupid. I used a liquid ms syringe purchased from a trusted vender. Sprayed everything down with 70 percent isopropyl alcohol. Used latex gloves also washed with alcohol and sprayed the inside of the sab with soapy water made sure there was no drafts and moved to not turn air flamed sterilized needle with torch and entered sab while needle was red hot.so youre saying 90 percent of suringes have contamns and need to be put through agar.
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Edited by Psyche delics (12/03/16 12:21 AM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: How xommon are contaminated syringes? [Re: Psyche delics]
#23889457 - 12/03/16 12:25 AM (7 years, 1 month ago) |
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good reply!
But yes I will say all spores should go to agar. Ive failed a lot in my grows. Once I started agar Ive had amazing results. Ms crapshots I say was like A 30% success rate?
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Psyche delics
Hypochondriac



Registered: 09/24/15
Posts: 3,644
Last seen: 4 months, 18 days
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Re: How xommon are contaminated syringes? [Re: mushboy]
#23889459 - 12/03/16 12:30 AM (7 years, 1 month ago) |
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Ok so when I do agar do I weed out contamn or do I have to just get lucky with a clean syringe. Because in all my agar work all Ive seen is contam.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: How xommon are contaminated syringes? [Re: Psyche delics]
#23889478 - 12/03/16 12:45 AM (7 years, 1 month ago) |
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Yes you clean up the culture before it gets to the jar.
Once the myc grows on the agar you look for fast growing leading edges to take a sample from. You put that to another plate and repeat.
Look up stros agar guide. I'm too lazy to link it ..on my fone
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Pinpapa
Stranger than most


Registered: 09/01/12
Posts: 259
Loc: Algonquin park
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Re: How xommon are contaminated syringes? [Re: mushboy]
#23889804 - 12/03/16 07:17 AM (7 years, 1 month ago) |
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If you want to eliminate failures ,this will kill!Agar will b a little like pulling wisdom teeth at first( ie. Painful )but its the only practical solution to contamination and wasted efforts.Imho.
-------------------- "This isn't the right thing to do , so let's go"
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