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Offlineafflictis
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Is this clean
    #23889192 - 12/02/16 10:13 PM (7 years, 1 month ago)

Made a few transfer it's a little fluffy on top but I do think see any other spots it's getting too close to the walls was going to either make more transfers or a blender less li



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InvisiblemushboyMDiscord
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Re: Is this clean [Re: afflictis]
    #23889200 - 12/02/16 10:17 PM (7 years, 1 month ago)

when using mini glads/rounds you dont have to worry about the 'growing to the sides thing' thats for petri dishes.

that uneven growth is weird. id take 1 more transfer to be sure. can always do that
and then do a LI or whatever with the rest of the plate:thumbup:


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Invisiblemorty422
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Re: Is this clean [Re: mushboy]
    #23889218 - 12/02/16 10:23 PM (7 years, 1 month ago)

Quote:

mushboy said:
when using mini glads/rounds you dont have to worry about the 'growing to the sides thing' thats for petri dishes.

that uneven growth is weird. id take 1 more transfer to be sure. can always do that
and then do a LI or whatever with the rest of the plate:thumbup:




:whathesaid:

I would do one more transfer to another plate. >then go to your blenderless LI or jars or what have you.

That uneven growth is strange at 11 o clock...


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Re: Is this clean [Re: morty422]
    #23889228 - 12/02/16 10:26 PM (7 years, 1 month ago)

wait i just realized I(and morty) told someone to use questionable growth
in a LI... id wait for a TC to judge that plate.

otherwise OP can be like 'fuck yall two i made 50 jars with that plate and all went to shit' zero rating for us both.:rofl:


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Offline99.99
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Re: Is this clean [Re: morty422]
    #23889231 - 12/02/16 10:27 PM (7 years, 1 month ago)

Maybe you didn't mix your agar very well


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Invisiblemorty422
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Re: Is this clean [Re: mushboy]
    #23889239 - 12/02/16 10:32 PM (7 years, 1 month ago)

Quote:

mushboy said:
wait i just realized I(and morty) told someone to use questionable growth
in a LI... id wait for a TC to judge that plate.

otherwise OP can be like 'fuck yall two i made 50 jars with that plate and all went to shit' zero rating for us both.:rofl:




I would be pretty confident in doing a LC with that growth after another transfer that shows good radial growth..

But..

Uh..

Yeah. Don't give me any albinos. I already had one asshole give me a 0 because I told him to quit telling people to not sterilize their grains.... haha


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Offlineafflictis
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Re: Is this clean [Re: morty422]
    #23889276 - 12/02/16 10:49 PM (7 years, 1 month ago)

That uneven growth at 11oclock was from transfers. Does the mycelium get a little fuzzy on top sometimes


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Re: Is this clean [Re: morty422]
    #23889307 - 12/02/16 11:03 PM (7 years, 1 month ago)

that clears it up LOLZ

that culture is good to go. i thought it ran into something and stop growing :facepalm:


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Offlineafflictis
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Re: Is this clean [Re: mushboy]
    #23889310 - 12/02/16 11:04 PM (7 years, 1 month ago)

Quote:

mushboy said:
when using mini glads/rounds you dont have to worry about the 'growing to the sides thing' thats for petri dishes.

that uneven growth is weird. id take 1 more transfer to be sure. can always do that
and then do a LI or whatever with the rest of the plate:thumbup:


what if there are droplets on the walls


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Offlineafflictis
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Re: Is this clean [Re: mushboy]
    #23889312 - 12/02/16 11:05 PM (7 years, 1 month ago)

Quote:

mushboy said:
that clears it up LOLZ

that culture is good to go. i thought it ran into something and stop growing :facepalm:


haha ok. I plan on doing a few more transfers so I have some more to make jars with but should I go ahead and make some li with it


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Re: Is this clean [Re: afflictis]
    #23889325 - 12/02/16 11:11 PM (7 years, 1 month ago)

oooh i gotcha by more transfers... i misread that like crazy.

the water droplets on the mini round are fine. annoying thats all.


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Offlineafflictis
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Re: Is this clean [Re: mushboy]
    #23889329 - 12/02/16 11:13 PM (7 years, 1 month ago)

Quote:

mushboy said:
oooh i gotcha by more transfers... i misread that like crazy.

the water droplets on the mini round are fine. annoying thats all.


yea I can't see insode had to set up the sab just to get a damn pic. Alright thanks for the advice


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Re: Is this clean [Re: afflictis]
    #23889368 - 12/02/16 11:27 PM (7 years, 1 month ago)

you can zap a plate in the microwave to heat up the agar inside and swish it
around. it will help with the water droplets. there are other things you
can do too but i dont remember them :frown:


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Offlineafflictis
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Re: Is this clean [Re: mushboy]
    #23889388 - 12/02/16 11:34 PM (7 years, 1 month ago)

Quote:

mushboy said:
you can zap a plate in the microwave to heat up the agar inside and swish it
around. it will help with the water droplets. there are other things you
can do too but i dont remember them :frown:


someone said you can put a hot cup on top and it will get rid of the condensation


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Re: Is this clean [Re: afflictis]
    #23889397 - 12/02/16 11:40 PM (7 years, 1 month ago)

:asianofapproval:


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Re: Is this clean [Re: mushboy]
    #23889434 - 12/03/16 12:07 AM (7 years, 1 month ago)

I would transfer that before making LI. Looks like a mold satellite sprung up right at the leading edge disrupting it. I would not use that myself.


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Offlineafflictis
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Re: Is this clean [Re: Pastywhyte]
    #23889438 - 12/03/16 12:12 AM (7 years, 1 month ago)

Quote:

Pastywhyte said:
I would transfer that before making LI. Looks like a mold satellite sprung up right at the leading edge disrupting it. I would not use that myself.


can you elaborate a little more.


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Re: Is this clean [Re: afflictis]
    #23889444 - 12/03/16 12:17 AM (7 years, 1 month ago)

A mold spore germinated on the plate outside of the transfer area. That is referred to as a satellite colony. Sometimes satellites are just cube mycelium if you dragged the wedge around making the transfer.  But sometimes they are random mold spores that flew in from your hands or tools. Better procedure elimitates those.

Only use plates that are clean as a whistle with healthy robust growth. Wimpy growth in an area is either mold or weak shitty cube genetics. Neither belong in your grain masters or LI jars.


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Invisiblemorty422
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Re: Is this clean [Re: Pastywhyte]
    #23889501 - 12/03/16 01:05 AM (7 years, 1 month ago)

Quote:

Pastywhyte said:
A mold spore germinated on the plate outside of the transfer area. That is referred to as a satellite colony. Sometimes satellites are just cube mycelium if you dragged the wedge around making the transfer.  But sometimes they are random mold spores that flew in from your hands or tools. Better procedure elimitates those.

Only use plates that are clean as a whistle with healthy robust growth. Wimpy growth in an area is either mold or weak shitty cube genetics. Neither belong in your grain masters or LI jars.




:shrug:

Pasty with the knowledge!

OP - You've got your answer...

Do another transfer yo!

Mush... too funny when he said that was a transfer area... lol... :lmafo:


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Offlineafflictis
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Re: Is this clean [Re: Pastywhyte]
    #23889519 - 12/03/16 01:19 AM (7 years, 1 month ago)

Quote:

Pastywhyte said:
I would transfer that before making LI. Looks like a mold satellite sprung up right at the leading edge disrupting it. I would not use that myself.


I did take transfers from it at 11oclock is that what you were talking about


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Re: Is this clean [Re: afflictis]
    #23889661 - 12/03/16 03:52 AM (7 years, 1 month ago)

Yes that's what he's talking about. Take transfers from the organized areas, not the uneven areas. Anywhere but that shit at 11 o'clock is what id be doing.


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Re: Is this clean [Re: Mad Season]
    #23889745 - 12/03/16 06:21 AM (7 years, 1 month ago)

No you need to transfer from a clean strong area to a fresh plate. Never try to cutout suspected contams. That just spreads mycelium fragments.


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InvisibleMad Season
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Re: Is this clean [Re: Pastywhyte]
    #23889939 - 12/03/16 08:44 AM (7 years, 1 month ago)

Oh is that what he was talking about lol. I thought he transferred that spot to a new plate, made me go wtf?


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Offlineafflictis
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Re: Is this clean [Re: Mad Season]
    #23890014 - 12/03/16 09:25 AM (7 years, 1 month ago)

Quote:

Mad Season said:
Oh is that what he was talking about lol. I thought he transferred that spot to a new plate, made me go wtf?



That spot at 11 was even growth I cut out mycelium from there already


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InvisibleMad Season
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Re: Is this clean [Re: afflictis]
    #23890023 - 12/03/16 09:27 AM (7 years, 1 month ago)

We're talking about the first pic right? How is that even? In the first pic 3-6 o'clock is what id call ideal, aggressive, and even


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Offlineafflictis
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Re: Is this clean [Re: Mad Season]
    #23890030 - 12/03/16 09:30 AM (7 years, 1 month ago)

Yes, that's how it looked I took some and made transfers the other day before I took those pics. That uneven even part looked just like the rest of the edges


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Offlineafflictis
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Re: Is this clean [Re: afflictis]
    #23890050 - 12/03/16 09:35 AM (7 years, 1 month ago)

This is how the plate looked a few days before I made transfers.


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Re: Is this clean [Re: afflictis]
    #23890076 - 12/03/16 09:45 AM (7 years, 1 month ago)

Okay now it makes sense. If that's a hole left from a previous transfer then go ahead. Looks weird cause you didn't cut right through the agar.


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Offlineafflictis
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Re: Is this clean [Re: Pastywhyte]
    #23890080 - 12/03/16 09:47 AM (7 years, 1 month ago)

Quote:

Pastywhyte said:
Okay now it makes sense. If that's a hole left from a previous transfer then go ahead. Looks weird cause you didn't cut right through the agar.


yea when I made these plates I made too much agar for how many plates I had so it's just really thick. I did notice the mycelium is a little fluffy on top is that normal


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Re: Is this clean [Re: afflictis]
    #23890083 - 12/03/16 09:49 AM (7 years, 1 month ago)

It looks fine to me.


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