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OfflineEl-Duderino
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Registered: 06/15/16
Posts: 21
Last seen: 8 years, 1 month
Sterile procedures prior to pc?
    #23885168 - 12/01/16 04:53 PM (8 years, 1 month ago)

Just PC'd r jars fill with rye. Followed RR's tek on the subject. I was assuming the PC would kill the contams already in the jar, but now in hindsight I'm paranoid. Took no sterile precautions prior to pc. Am I justifiably nervous?

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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
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Re: Sterile procedures prior to pc? [Re: El-Duderino]
    #23885172 - 12/01/16 04:55 PM (8 years, 1 month ago)

Sounds like you are straight fucked

Sterilization kills everything :lol: you're worrying over nothing.


--------------------
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InvisiblemushboyMDiscord
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Registered: 04/24/05
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Re: Sterile procedures prior to pc? [Re: Mad Season]
    #23885179 - 12/01/16 04:58 PM (8 years, 1 month ago)

fear not!!

some steps of cult are not sterile. like fruiting.

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InvisibleNobler Hino
a dojo and a forge?!
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Registered: 08/29/15
Posts: 1,785
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Re: Sterile procedures prior to pc? [Re: Mad Season]
    #23885182 - 12/01/16 04:59 PM (8 years, 1 month ago)

Lol, paranoia is key


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina

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OfflineEl-Duderino
Nihilist


Registered: 06/15/16
Posts: 21
Last seen: 8 years, 1 month
Re: Sterile procedures prior to pc? [Re: Mad Season]
    #23885198 - 12/01/16 05:07 PM (8 years, 1 month ago)

So what exactly is the PC doing if not killing all the contams

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OfflineEl-Duderino
Nihilist


Registered: 06/15/16
Posts: 21
Last seen: 8 years, 1 month
Re: Sterile procedures prior to pc? [Re: El-Duderino]
    #23885206 - 12/01/16 05:10 PM (8 years, 1 month ago)

Oh wow I didn't see your second message haha!

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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,348
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Re: Sterile procedures prior to pc? [Re: Mad Season]
    #23885371 - 12/01/16 06:15 PM (8 years, 1 month ago)

Quote:

Mad Season said:
Sounds like you are straight fucked

Sterilization kills everything :lol: you're worrying over nothing.



it doesn't kill everything. bacterial endospores are just damaged, and will recover from a pc cycle in roughly 2 weeks. when we PC, we create a window of opportunity to introduce our cultures before the endospores bounce back.

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InvisibleMad Season
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Registered: 09/16/12
Posts: 12,666
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Re: Sterile procedures prior to pc? [Re: Munchauzen]
    #23885383 - 12/01/16 06:19 PM (8 years, 1 month ago)

Lol it definitely kills everything he would introduce prior to the PC, but yeah I do agree with you. I was debating on saying that I was lying because of endos but thought for this guy he doesn't need to worry.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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