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Sk8nshram
pigskin footballs



Registered: 11/01/13
Posts: 1,089
Loc: PNW
Last seen: 1 hour, 13 minutes
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: DavidReishi]
#23884191 - 12/01/16 10:56 AM (7 years, 2 months ago) |
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Quote:
DavidReishi said: The only way you'd get as fast or faster colonization from a whole mushroom than a stem-butt is if the whole mushroom still has the stem-butt attached. Don't forget, just a couple weeks ago you were still dispensing the advice to blend fresh mushrooms into a liquid and spray it on woodchips, a process that doesn't work at all.
True. This process could work to start a patch, but it's inefficient. The spores will need time to germinate, this could take years to actually produce fruiting bodies. With stem butts you already have live culture. I will blend up rotten fruits and dump them but never good ones.
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: Sk8nshram]
#23884241 - 12/01/16 11:21 AM (7 years, 2 months ago) |
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You made me realize that my last statement was inaccurate. Blending fresh mushrooms and applying to woodchips doesn't work as far as the transfer of mycelial growth is concerned. But if matured caps were included in the blend, then presumably the application might eventually work via the spores.
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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Ran-D



Registered: 12/19/10
Posts: 16,318
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: DavidReishi]
#23884264 - 12/01/16 11:33 AM (7 years, 2 months ago) |
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What a delusional little girl. ^^^
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Byrain

Registered: 01/07/10
Posts: 9,664
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: DavidReishi]
#23884268 - 12/01/16 11:35 AM (7 years, 2 months ago) |
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I was going to reply, but then I saw this... LOL.
Quote:
DavidReishi's signature said:Jealous Stay-at-homes being ignored: Mr Piggy, Groo, maynardjameskeenan, Ran-D, Byrain, afrogus
For anyone else reading, it does not really matter if the mushroom is fresh or not when trying to make a culture. Blending up mushrooms and spraying them on wood chips can work, but this is an inefficient process. Using non-fresh mushrooms may result in a greater risk of contamination.
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: DavidReishi]
#23884276 - 12/01/16 11:38 AM (7 years, 2 months ago) |
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Lol, "might eventually" pertains to the Bay area; for the PNW, replace with "would likely."
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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Sirtalis



Registered: 10/05/16
Posts: 409
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: DavidReishi]
#23884435 - 12/01/16 12:52 PM (7 years, 2 months ago) |
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You guys are misinterpreting my agar experiments. Tissue taken from the gills grows faster than tissue taken from stem butts. Same mass of tissue. These are not spores germinating rather mycelium growing out of the gills.
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: Sirtalis]
#23884445 - 12/01/16 01:00 PM (7 years, 2 months ago) |
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I know what you meant. Still, put a fresh whole mushroom without a stem-butt in woodchips, and put a stem-butt in woodchips. Then watch the later colonize the woodchips much faster.
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: DavidReishi]
#23884468 - 12/01/16 01:09 PM (7 years, 2 months ago) |
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Not to mention, "tissue taken from a stem-butt" isn't the same thing as a stem-butt.
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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Sk8nshram
pigskin footballs



Registered: 11/01/13
Posts: 1,089
Loc: PNW
Last seen: 1 hour, 13 minutes
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: DavidReishi]
#23884491 - 12/01/16 01:23 PM (7 years, 2 months ago) |
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I say if there is a way to propagate more patches, do it. Stem butt, multispore culture, blended fruits, lc. Do as much as you can to spread that love and always experiment with new processes. Sometime the clandestine noob methods turn out the best. Just plain ol spores suspended in water and sprayed within 24 hrs has worked well for me and a few others I know.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: Sk8nshram]
#23884505 - 12/01/16 01:28 PM (7 years, 2 months ago) |
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Quote:
DavidReishi said: just a couple weeks ago you were still dispensing the advice to blend fresh mushrooms into a liquid and spray it on woodchips, a process that doesn't work at all.
Why wouldn't this work? I've picked and buried fresh mushrooms in new locationa and it works to spread the patch.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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Sirtalis



Registered: 10/05/16
Posts: 409
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: Byrain]
#23884506 - 12/01/16 01:29 PM (7 years, 2 months ago) |
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Why not just do it proper? Clone to agar and make grain spawn.
Quote:
DavidReishi's signature said:Jealous Stay-at-homes being ignored: Mr Piggy, Groo, maynardjameskeenan, Ran-D, Byrain, afrogus
Also, how do I get onto the list of all-stars?
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: Sirtalis]
#23884536 - 12/01/16 01:40 PM (7 years, 2 months ago) |
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Quote:
Sirtalis said:
Why not just do it proper? Clone to agar and make grain spawn.
I think you're confusing indoor and outdoor cultivation. Going from unsterile mushroom to sterile agar, then to sterile grains, just to go back to unsterile woodchips, is like departing SF on a plane and flying around the world just to arrive in San Jose.
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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Sk8nshram
pigskin footballs



Registered: 11/01/13
Posts: 1,089
Loc: PNW
Last seen: 1 hour, 13 minutes
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: DavidReishi]
#23884555 - 12/01/16 01:48 PM (7 years, 2 months ago) |
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Quote:
DavidReishi said:
Quote:
Sirtalis said:
Why not just do it proper? Clone to agar and make grain spawn.
I think you're confusing indoor and outdoor cultivation. Going from unsterile mushroom to sterile agar, then to sterile grains, just to go back to unsterile woodchips, is like departing SF on a plane and flying around the world just to arrive in San Jose.
Not true, the amount of time to make a patch from agar done correctly is way faster and more proficient. It can guarantee fruits the following season. Planting stem butts and spraying spore water are not guaranteed to produce fruits as soon. Have you done any agar work before?
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Sirtalis



Registered: 10/05/16
Posts: 409
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: Sirtalis]
#23884570 - 12/01/16 01:52 PM (7 years, 2 months ago) |
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https://www.shroomery.org/forums/showflat.php/Number/17038273#17038273
"But as already said, it's far better to expand your grains into sawdust indoors over the winter, and then use that to inoculate sawdust, bark and chips in outdoor beds in the spring in hopes it will colonize enough to fruit by the following fall. RR"
Edited by Sirtalis (12/14/16 11:14 PM)
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Loveshroomz777


Registered: 11/19/16
Posts: 616
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: Sk8nshram]
#23884573 - 12/01/16 01:53 PM (7 years, 2 months ago) |
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How long does it take for a spot to fruit after transferring stem butts can someone please elaborate on the process I am interested in doing it for next year got to start making things happen
-------------------- PEACE, LOVE AND PSYCHEDELICS
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Sirtalis



Registered: 10/05/16
Posts: 409
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: Loveshroomz777]
#23884581 - 12/01/16 01:54 PM (7 years, 2 months ago) |
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: Sk8nshram]
#23884593 - 12/01/16 01:57 PM (7 years, 2 months ago) |
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Sk8nshram - You're probably right when it comes to planting stem-butts...though perhaps not if you had as many stem-butts as rye grains. But what I'm referring to is spawning vis-a-vis stem-butts, then making an outdoor bed with the spawned woodchips and new woodchips.
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: Sk8nshram]
#23884629 - 12/01/16 02:11 PM (7 years, 2 months ago) |
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Here's an example. P. azurescens mycelium colonizing woodchips from a stem-butt.
And, yes, l've worked with agar.
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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Sk8nshram
pigskin footballs



Registered: 11/01/13
Posts: 1,089
Loc: PNW
Last seen: 1 hour, 13 minutes
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: DavidReishi]
#23884632 - 12/01/16 02:12 PM (7 years, 2 months ago) |
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Quote:
DavidReishi said: Sk8nshram - You're probably right when it comes to planting stem-butts...though perhaps not if you had as many stem-butts as rye grains. But what I'm referring to is spawning vis-a-vis stem-butts, then making an outdoor bed with the spawned woodchips and new woodchips.
Yeah i understand. Ime some some stem butts will colonize very well, and others will die. So if you have extremely healthy stem butts I'm sure you can get a patch going pretty well. Id say about 50 to 75 percent of my stem butt spawn spots have worked.
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: OFFICIAL SAN FRANCISCO BAY AREA ACTIVES 2016 [Re: Sirtalis] 1
#23884679 - 12/01/16 02:32 PM (7 years, 2 months ago) |
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Quote:
Sirtalis said:

Try making gallons of spawn out of stem butts and let me know how long it takes.

Will do.
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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