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Offlinetriangle
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Registered: 06/25/11
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Mycelium in syringe?
    #23882267 - 11/30/16 04:21 PM (7 years, 1 month ago)

I have a syringe of "Wavy Caps" P. Cyanescens. When I got it a month ago it was a normal looking syringe no clumps just spores. I left it in a the closet for storage and started to notice the spores all clump up together I tried shaking it but they did not disperse and now there is a ball of cloud. Im guessing its just mycelium..what do you guys think?

Im just going to inoculate some jars and see what happens or do you guys think I should take that chunk of cloud and put it in an petri dish with agar?







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Offlineacidninja
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Re: Mycelium in syringe? [Re: triangle]
    #23882288 - 11/30/16 04:30 PM (7 years, 1 month ago)

Yer I had the same problem with my oyster syringes, tryin to get it out will probs just kill it, I tried using em', nothing, I suggest tossin' em or just getting lucky and squirtin' em into your composter :shgrug:


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Edited by acidninja (11/30/16 04:31 PM)


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InvisibleMush 4 Brains
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Re: Mycelium in syringe? [Re: triangle]
    #23882289 - 11/30/16 04:31 PM (7 years, 1 month ago)

Personally i dont trust ms syringes from vendors or even my own enough to go straight to grains. Always agar, no exception.

You have one fully colonized jar from ms syringe with some hidden slow moving contam and you use it to g2g a shit load and a week or so later you're crying over all the wasted time/materials. It only happened a few times over a decade of growing but that was enough for me to never chance it.


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
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Re: Mycelium in syringe? [Re: acidninja]
    #23882295 - 11/30/16 04:34 PM (7 years, 1 month ago)

I don't see why spores would germinate in distilled water. Why not inoculate some petri dishes?


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InvisibleThadeous
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Registered: 08/02/11
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Re: Mycelium in syringe? [Re: Dactylium]
    #23882359 - 11/30/16 05:01 PM (7 years, 1 month ago)

It happens sometimes. Temperature and maybe air getting into the syringe AFAIK. If it isn't a contaminate it's safe to use. Liquid cultures work in a similar fashion.


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Offlineacidninja
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Re: Mycelium in syringe? [Re: Thadeous]
    #23882434 - 11/30/16 05:24 PM (7 years, 1 month ago)

I keep my syringes in the fridge, always, they last for months in there. *cough* *cough* uses 11 year old syringes *couch*


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InvisibleThadeous
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Registered: 08/02/11
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Re: Mycelium in syringe? [Re: acidninja]
    #23882465 - 11/30/16 05:31 PM (7 years, 1 month ago)

:rofl: I recently went through a purge and got rid of dozens. Dunno how they amalgamated.

I'd inoculate and see what happens. Should be faster since it's past the spore stage.


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Offlinenolongrlurkin
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Re: Mycelium in syringe? [Re: Thadeous]
    #23886112 - 12/01/16 10:34 PM (7 years, 1 month ago)

A friend of mine, really, ordered some and left em in his vehicle in florida, summertime, for almost a month. I don't know how they looked when he received them but when he gave them to me there were cloudy masses in all the syringes, a.p.e., malibar and Chitwan. 24 knocked up jars later, the spores are safe now in the fridge, 2/3rds at least.


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Offlineblackout
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Re: Mycelium in syringe? [Re: Dactylium]
    #24001387 - 01/11/17 02:30 PM (7 years, 19 days ago)

Quote:

Dactylium said:
I don't see why spores would germinate in distilled water. Why not inoculate some petri dishes?



one theory is rarer spores would be more likely to have gill fragments in them, and that they can "cannibalise" themselves. I have seen apparent myc in syringes before, usually in non-cubensis.

I would put the cloud in the first post on agar, you can let it settle needle down so it falls to the bottom and is first out.


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