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OfflineDabrit
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Noob question regarding timing your spawn jars to be spawned to bulk monotubs
    #23881762 - 11/30/16 01:52 PM (7 years, 1 month ago)

Before I start I'll state I did try and search for this answer but didn't exactly get the answer I was looking for.

I am wondering how you guys go about getting the timing just right so that your 6-7 quart jars of spawn are all ready at the same time so that they can be spawned to bulk monotubs(In my situation I'm spawning to coir/verm)

1. inoculate perhaps 2-3 agar petri dishes via spore syringe with one variety, decide which one has the best mycelium growth and then grow that out till almost a 1/4 inch from the edge of the petri dish and divide that between your 6-7 spawn jars.

Or

2. inoculate 2-3 agar petri dishes via spore syringe wait to see which has the best growth only transfer agar to one master jar wait until it fully colonizes then use it to grain to grain say 8 quart jars( 2 extra as a buffer in case of contamination) ?

From there eat one of the largest nicest looking fruits make sure it is actually potent and not a bunk batch then clone to agar and use as a master petri dish to start the cycle all over again via grain to grain?


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




Edited by Dabrit (11/30/16 01:58 PM)


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OfflineJabensis
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Dabrit]
    #23881780 - 11/30/16 02:00 PM (7 years, 1 month ago)

The "best" mycelium growth does not necessarily = a "good" fruiting strain.

My advice to you, friend is to clone a fruit on agar, then take to grain, then spawn your sub.
The clone will theoretically grow at equal rates in each jar.

Your timing issue should only appear during a MS (multi-spore) grow whereas cloners grow the same.
Regardless, your only looking at a few days difference for MS colonization.

Hope this answers it.


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J


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InvisibleKush_Zombie
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Jabensis]
    #23881802 - 11/30/16 02:07 PM (7 years, 1 month ago)

In case this is part of your question as well, I've let fully colonized jars sit for a good month before I finally spawned them to a tub. I just fill the jars up with water and let them sit for about 10 minutes to rehydrate my spawn and then drain the water and throw the hydrated spawn into a tub.
What I'm getting at with this is that they don't have to all colonize at the exact same time, you have a bit of time to just let them sit and chill while the rest of the jars catch up.


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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InvisiblemushboyMDiscord
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Dabrit]
    #23881828 - 11/30/16 02:16 PM (7 years, 1 month ago)

Quote:

Dabrit said:
...eat one of the largest nicest looking fruits make sure it is actually potent and not a bunk batch then clone to agar and use as a master petri dish to start the cycle all over again via grain to grain?




I like to do this but from a MS grow. it can be tricky with clean spawn but you will get more desirable and varied results.


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Offlineno0bsawse
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Registered: 08/26/16
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Dabrit]
    #23881829 - 11/30/16 02:16 PM (7 years, 1 month ago)

I don't think there is a thing as a "bunk batch." When using MS, you are using all kinds of different dna so that every mushroom is different, when you clone, well... you clone. So using MS in theory you could pick a little baby mushroom and it be potent, or pick a big one and have it be weak. I'm currently using MS and in order to keep my experiences consistent, I try to eat a mixture of big and small fruits.


--------------------
LF: Gourmet Strains


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InvisibleKush_Zombie
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: mushboy]
    #23881842 - 11/30/16 02:20 PM (7 years, 1 month ago)

Quote:

mushboy said:
Quote:

Dabrit said:
...eat one of the largest nicest looking fruits make sure it is actually potent and not a bunk batch then clone to agar and use as a master petri dish to start the cycle all over again via grain to grain?




I like to do this but from a MS grow. it can be tricky with clean spawn but you will get more desirable and varied results.



You like to clone mushrooms that you've eaten first?

:strokebeard: care to elaborate?


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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InvisiblemushboyMDiscord
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Posts: 32,281
Loc: where?
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Kush_Zombie]
    #23881849 - 11/30/16 02:22 PM (7 years, 1 month ago)

you take a tissue sample. put on plate. eat.

if you decide not to grow just toss the plate. or grow out if you liked it...


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InvisibleKush_Zombie
smug piece of shit
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: mushboy]
    #23881855 - 11/30/16 02:25 PM (7 years, 1 month ago)

Oh, he said to eat it and THEN take the clones :lol:
Sorry, I'm just giving you shit


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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OfflineDabrit
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Registered: 12/02/15
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Loc: Pacific Northwest
Last seen: 6 months, 29 days
Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Kush_Zombie]
    #23883435 - 12/01/16 12:28 AM (7 years, 1 month ago)

Quote:

Kush_Zombie said:
Oh, he said to eat it and THEN take the clones :lol:
Sorry, I'm just giving you shit




Yeah obviously I meant take a tissue sample before eating the rest of the fruit.


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Dabrit]
    #23883457 - 12/01/16 12:44 AM (7 years, 1 month ago)

If you use liquid inoculant you should see your jars finish colonizing at about the same time even if it's an MS grow. It doesn't really matter though.


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OfflineDabrit
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: no0bsawse]
    #23885102 - 12/01/16 04:34 PM (7 years, 1 month ago)

Quote:

no0bsawse said:
I don't think there is a thing as a "bunk batch." When using MS, you are using all kinds of different dna so that every mushroom is different, when you clone, well... you clone. So using MS in theory you could pick a little baby mushroom and it be potent, or pick a big one and have it be weak. I'm currently using MS and in order to keep my experiences consistent, I try to eat a mixture of big and small fruits.




Apparently the B+ variety have a reputation for having " bunk batches. " Seen as I split a 1/2oz between me and some friends to trip for all of us not to trip shows something was definitely up with the batch and not just a fruit or two.


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OfflineMushierage
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Dabrit]
    #23885136 - 12/01/16 04:43 PM (7 years, 1 month ago)

B+ is one I do consistently and I've never ever had any issues with potency.  I like B+ because it's one of the most hardy variants I've worked with.  Even when the sub/spawn is bacterial as hell, it always manages to give me at least a couple flushes and only slightly reduced yields. 

If you are doing multispore, then you'll always get average potency mush from my experience.  I've never had B+ that was extremely high potency, always just average or slightly above average.  What varies for me is the speed of colonization and the size and yield of fruits. 

If you want high potency, strong colonization and high yield, you need to be isolating and for any type of consistency taking clones from various MS grows.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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InvisibleMad Season
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Dabrit]
    #23885196 - 12/01/16 05:05 PM (7 years, 1 month ago)

Quote:

Dabrit said:
Quote:

no0bsawse said:
I don't think there is a thing as a "bunk batch." When using MS, you are using all kinds of different dna so that every mushroom is different, when you clone, well... you clone. So using MS in theory you could pick a little baby mushroom and it be potent, or pick a big one and have it be weak. I'm currently using MS and in order to keep my experiences consistent, I try to eat a mixture of big and small fruits.




Apparently the B+ variety have a reputation for having " bunk batches. " Seen as I split a 1/2oz between me and some friends to trip for all of us not to trip shows something was definitely up with the batch and not just a fruit or two.



This shit has happened to me for almost every variety, as well as the opposite from different grows of the same variety.

I've had good and bad golden teachers, Orissa India, B+, Ecuador, Blue Meanies, Cambodian, creepers, treasure coast.. the only strains I've worked with that didn't have any bunk shit is PE and Thai lipa yai, and even then they both have had complaints on here numerous times that they're bunk.

Saying a variety does something is like saying all black people like chicken and watermelon, or all Mexicans are lazy. Every person is different, just like every time 2 spores meet is different.

B+ is like one of the goto varieties for noobs. Of course it'll have much more documentation of biases


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineDabrit
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Mushierage]
    #23885333 - 12/01/16 05:57 PM (7 years, 1 month ago)

Quote:

Mushierage said:
B+ is one I do consistently and I've never ever had any issues with potency.  I like B+ because it's one of the most hardy variants I've worked with.  Even when the sub/spawn is bacterial as hell, it always manages to give me at least a couple flushes and only slightly reduced yields. 

If you are doing multispore, then you'll always get average potency mush from my experience.  I've never had B+ that was extremely high potency, always just average or slightly above average.  What varies for me is the speed of colonization and the size and yield of fruits. 

If you want high potency, strong colonization and high yield, you need to be isolating and for any type of consistency taking clones from various MS grows.




When I grew B+ via PF tek they were good, but my first monotub run via coir/verm turned out to be bunk.

If the spores in a syringe are say all coming from one specific fruit would that not perhaps suggest the genetics of that fruit are transferred to all the spores it produces. So say one spore syringe of B+ is all from one fruit which just so happens to be a bunk fruit then the whole batch could be bunK?


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OfflineMushierage
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Dabrit]
    #23885505 - 12/01/16 07:07 PM (7 years, 1 month ago)

That's a question for somebody more experienced with genetics, so I can't answer that question.  But you are on the right track when you are talking about potency being directly related intially to genetics.  Secondary detractors of potency can be anything from the nutrients available in your grow to the way you dry or prep your fruits. 

I'm sure there are other things that can reduce potency, but genetics control what is possible.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineDabrit
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Mushierage]
    #23887962 - 12/02/16 03:17 PM (7 years, 1 month ago)

Quote:

Mushierage said:
That's a question for somebody more experienced with genetics, so I can't answer that question.  But you are on the right track when you are talking about potency being directly related intially to genetics.  Secondary detractors of potency can be anything from the nutrients available in your grow to the way you dry or prep your fruits. 

I'm sure there are other things that can reduce potency, but genetics control what is possible.




Yeah the only thing I can think of other than genetics that may have caused loss of potency was the fact that due to the consolidation of the mycelium in my jars being so strong I could break up the grains by whacking the jars on a phone book. I actually had to use a knife and cut up the mycelium in to chunks and then dumped out before spawning to coir/verm.

The flush was huge honestly didn't expect to do so well on my first time around( granted I did use 7 quarts of spawn) but even though the fruits looked great all of the ones we ate basically did nothing other than very mild visuals like if you were to eat maybe .5 of a gram and we all ate around 3 to 3.5. Also I should make mention of the fact that the jars took about 2 months to fully colonize for some reason. Perhaps there was invisible bacteria I couldn't see that affected the potency or perhaps longer colonization times itself equals lower potency.

Speaking of slow colonization, used the same spore syringes to knock up 4 agar dishes 9 days ago now so far no signs of growth at all. Should I just toss the rest of the syringe you think? It's over a year old now


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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InvisibleMad Season
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Dabrit]
    #23887985 - 12/02/16 03:22 PM (7 years, 1 month ago)

Quote:

Dabrit said:
Yeah the only thing I can think of other than genetics that may have caused loss of potency was the fact that due to the consolidation of the mycelium in my jars being so strong I could break up the grains by whacking the jars on a phone book. I actually had to use a knife and cut up the mycelium in to chunks and then dumped out before spawning to coir/verm.



That sounds so bacterial it ain't even funny. Clean spawn breaks up with a few shakes.

Quote:

Dabrit said:
Also I should make mention of the fact that the jars took about 2 months to fully colonize for some reason.



If you don't have bacteria you might as well just ship me off to mycotopia
Quote:

Dabrit said:
Perhaps there was invisible bacteria



:yes:
Quote:

Dabrit said:
I couldn't see that affected the potency or perhaps longer colonization times itself equals lower potency.



I don't see how it would affect potency either, considering if you dig around there's some people who talk how their bacterial grows destroyed them.
Quote:

Dabrit said:
Speaking of slow colonization, used the same spore syringes to knock up 4 agar dishes 9 days ago now so far no signs of growth at all. Should I just toss the rest of the syringe you think? It's over a year old now



9 days is nothing.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineDabrit
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Mad Season]
    #23888045 - 12/02/16 03:44 PM (7 years, 1 month ago)

Quote:

Mad Season said:
Quote:

Dabrit said:
Yeah the only thing I can think of other than genetics that may have caused loss of potency was the fact that due to the consolidation of the mycelium in my jars being so strong I could break up the grains by whacking the jars on a phone book. I actually had to use a knife and cut up the mycelium in to chunks and then dumped out before spawning to coir/verm.



That sounds so bacterial it ain't even funny. Clean spawn breaks up with a few shakes.

Quote:

Dabrit said:
Also I should make mention of the fact that the jars took about 2 months to fully colonize for some reason.



If you don't have bacteria you might as well just ship me off to mycotopia
Quote:

Dabrit said:
Perhaps there was invisible bacteria



:yes:
Quote:

Dabrit said:
I couldn't see that affected the potency or perhaps longer colonization times itself equals lower potency.



I don't see how it would affect potency either, considering if you dig around there's some people who talk how their bacterial grows destroyed them.
Quote:

Dabrit said:
Speaking of slow colonization, used the same spore syringes to knock up 4 agar dishes 9 days ago now so far no signs of growth at all. Should I just toss the rest of the syringe you think? It's over a year old now



9 days is nothing.




Yeah I was thinking something for sure was up with 2 months colonization times. I was just directly inoculating my grain jars with a ms syringe so like a bunch of peeps on here have been telling me agar is the only way to go. I did do my first batch the pastyplate tek method but I'm having a really hard time seeing into the containers a) because the lids are blue as is the colour of the agar due to the food colouring I chose and also condensation inside of the containers as well.

I just received my first 20 pack of sterilized petri dishes for my next project hopefully my PE and GT syringes should arrive soon and I will do some pouring. How many would you suggest knocking up at one time on agar with the syringe? Just maybe 2 per variety at first then clean up from there?

Also curious as to why people put saran wrap around the edges of their colonizing petri dishes, doesn't mycelium need Gas exchange to occur? I get wrapping unused poured dishes but ones which are growing what gives?


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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InvisibleMad Season
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Dabrit]
    #23888060 - 12/02/16 03:47 PM (7 years, 1 month ago)

I always do 2-4 plates per variety for germination. Sounds like you're on the right track :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineDabrit
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Re: Noob question regarding timing your spawn jars to be spawned to bulk monotubs [Re: Mad Season]
    #23888084 - 12/02/16 03:54 PM (7 years, 1 month ago)

Quote:

Mad Season said:
I always do 2-4 plates per variety for germination. Sounds like you're on the right track :smile:




Thanks man. Appreciate all the insight.

Everyday I feel I'm learning something new in the world of mycology, I really hope in the future I'll be able to expand my knowledge and get a larger room for my projects.

PF Tek already seems like such a distant memory but it was only a year ago.


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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