|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Booooooooom
Stranger
Registered: 11/16/15
Posts: 139
Last seen: 6 months, 23 days
|
Monotub ready or not?
#23881251 - 11/30/16 11:05 AM (7 years, 1 month ago) |
|
|
Its been over a week(10 days) since the monotub started colonizing. Its been a while since i did a tub. So im wondering how white the surface is supposed to look before i can put it in fruting.
|
egobrain


Registered: 11/17/16
Posts: 180
Loc: Canada
|
|
That is a really terrible picture. From what I can see it doesn't look fully colonized.
Is this in open air? A bag? Need more info.
Edited by egobrain (11/30/16 11:19 AM)
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Monotub ready or not? [Re: egobrain]
#23881261 - 11/30/16 11:11 AM (7 years, 1 month ago) |
|
|
i think i see bigfoot..
seriously though. need a better pic.
|
JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
|
Re: Monotub ready or not? [Re: egobrain]
#23881268 - 11/30/16 11:13 AM (7 years, 1 month ago) |
|
|
Its a tub. Full colonization isnt required for fruiting.
OP it could take a long time to fruit. Pasty once said he had a tub of PE go like 2-3 months.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: Monotub ready or not? [Re: mushboy]
#23881271 - 11/30/16 11:13 AM (7 years, 1 month ago) |
|
|
Take a pic with the lid off. I hope my eyes are deceiving me and there isn't a shit ton of green in there.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Monotub ready or not? [Re: Mad Season]
#23881277 - 11/30/16 11:16 AM (7 years, 1 month ago) |
|
|
Quote:
Mad Season said: ...there isn't a shit ton of green in there.
i thought the same thing!! without moving the pic right in the middle looks ..ehhh???
|
egobrain


Registered: 11/17/16
Posts: 180
Loc: Canada
|
Re: Monotub ready or not? [Re: mushboy]
#23881287 - 11/30/16 11:19 AM (7 years, 1 month ago) |
|
|
Holy shit trich alert.
|
Booooooooom
Stranger
Registered: 11/16/15
Posts: 139
Last seen: 6 months, 23 days
|
Re: Monotub ready or not? [Re: egobrain]
#23881303 - 11/30/16 11:28 AM (7 years, 1 month ago) |
|
|
Its a shit ton of green in there  I tossed that shit out
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|
Yep that's what I thought lol
|
PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
|
|
That shit should have been in the trash a week ago
|
Booooooooom
Stranger
Registered: 11/16/15
Posts: 139
Last seen: 6 months, 23 days
|
|
Wtf went wrong the jars were all fine one had little yellow liquid but thats okey i think?. I did damons coir tek as bulk. And i taped a black bag on the inside with tape. What went wrong?.. now i have to start all over again..
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 11 hours
|
|
Quote:
Booooooooom said: Its been over a week(10 days) since the monotub started colonizing. Its been a while since i did a tub. So im wondering how white the surface is supposed to look before i can put it in fruting.

The moon is close tonight
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|
Well if ya gotta start over again, start right and do agar my friend.
Lol if one jar had a yellow spot in it, I can almost guarantee that lots of your jars had bacteria in them.
|
krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
|
Re: Monotub ready or not? [Re: Mad Season]
#23882806 - 11/30/16 07:37 PM (7 years, 1 month ago) |
|
|
Looks like a big blotch of green shit to me. If you get yellow shit in your jar it's probably due to bacteria which makes the mycilium weak enough to be taken by trich once spawned.
|
Booooooooom
Stranger
Registered: 11/16/15
Posts: 139
Last seen: 6 months, 23 days
|
Re: Monotub ready or not? [Re: krypto2000]
#23900395 - 12/06/16 04:13 PM (7 years, 1 month ago) |
|
|
So you should nerver use yellowed jars then?
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
|
for cubes...no i would suggest to just toss them and use healthy spawn
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
|
Re: Monotub ready or not? [Re: cronicr]
#23900498 - 12/06/16 04:48 PM (7 years, 1 month ago) |
|
|
I wouldn't. Imo if a jar is producing a noticable amount of yellow metabolites then if it's not contaminated it's very old and has been sitting for awhile.
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 11 hours
|
Re: Monotub ready or not? [Re: krypto2000]
#23902031 - 12/07/16 12:55 AM (7 years, 1 month ago) |
|
|
If you are desperate you can straight case them and get a flush or 2. You have to remember people who are into agar have 100s of jars on the go all the time. If you have 2 and the have slight yellowing and need some mushies, st8 case is the only chance you have.
|
plurfekt
Finally Grateful

Registered: 12/14/09
Posts: 1,919
Loc: USA
|
Re: Monotub ready or not? [Re: cronicr]
#23902062 - 12/07/16 01:23 AM (7 years, 1 month ago) |
|
|
Quote:
cronicr said: for cubes...no i would suggest to just toss them and use healthy spawn

PE does take a while longer, but if it's not clean, it's no bueno regardless.
I throw anything funky just to preserve the grow area itself, if nothing else.
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 11 hours
|
Re: Monotub ready or not? [Re: plurfekt]
#23902211 - 12/07/16 04:24 AM (7 years, 1 month ago) |
|
|
Preserving the grow area from what? Most grow areas are as dirty as fuk unless they have been bleached and had an hepa filter running for a day.
My point is only this. If you are a noob and you only have 2 jars its worth straight casing as firstly you get to know what contaminants look like if they appear and secondly you may get some mushrooms in a week instead of a month.
Most Mold Ive encountered doesnt produce spores till it goes green and so if the white we all hate shows up, sling it.
However, if you have lots of jars or or a stash of mushies then go toss them out.
|
plurfekt
Finally Grateful

Registered: 12/14/09
Posts: 1,919
Loc: USA
|
Re: Monotub ready or not? [Re: Edmunter]
#23902263 - 12/07/16 05:00 AM (7 years, 1 month ago) |
|
|
I do bleach my grow area, and keep a HEPA filter running.
I stand by removing contamination from the equation, but too each their own.
When I learned beginners mycology, I wanted to learn it properly.
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 11 hours
|
Re: Monotub ready or not? [Re: plurfekt]
#23902371 - 12/07/16 06:44 AM (7 years, 1 month ago) |
|
|
Read this
https://www.shroomery.org/forums/showflat.php/Number/19722262
I rate Frank as one of the best growers on the planet and when he gets out of jail or wherever he is he will tell you that he lives in a grotty old apartment with dogs and ferrets and its full of contaminants. He always stresses the only place you need to keep clean is your sterile area and that is not your grow room.
Why are you bleaching your grow area as I am truly interested?
Edited by Edmunter (12/07/16 06:59 AM)
|
krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
|
Re: Monotub ready or not? [Re: Edmunter]
#23902612 - 12/07/16 08:59 AM (7 years, 1 month ago) |
|
|
I can see green spores all over the mono in that pic.
|
krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
|
Re: Monotub ready or not? [Re: krypto2000]
#23902878 - 12/07/16 10:48 AM (7 years, 1 month ago) |
|
|
Frank is in jail? I wondered where he's been. What happened?
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 11 hours
|
|
Im kidding around
|
plurfekt
Finally Grateful

Registered: 12/14/09
Posts: 1,919
Loc: USA
|
Re: Monotub ready or not? [Re: Edmunter]
#23903053 - 12/07/16 11:42 AM (7 years, 1 month ago) |
|
|
Quote:
Edmunter said: Read this
https://www.shroomery.org/forums/showflat.php/Number/19722262
I rate Frank as one of the best growers on the planet and when he gets out of jail or wherever he is he will tell you that he lives in a grotty old apartment with dogs and ferrets and its full of contaminants. He always stresses the only place you need to keep clean is your sterile area and that is not your grow room.
Why are you bleaching your grow area as I am truly interested?
Suppose you never considered my grow area and my work area, were in the same area...
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 11 hours
|
Re: Monotub ready or not? [Re: plurfekt]
#23903088 - 12/07/16 11:52 AM (7 years, 1 month ago) |
|
|
Quote:
plurfekt said:
Quote:
Edmunter said: Read this
https://www.shroomery.org/forums/showflat.php/Number/19722262
I rate Frank as one of the best growers on the planet and when he gets out of jail or wherever he is he will tell you that he lives in a grotty old apartment with dogs and ferrets and its full of contaminants. He always stresses the only place you need to keep clean is your sterile area and that is not your grow room.
Why are you bleaching your grow area as I am truly interested?
Suppose you never considered my grow area and my work area, were in the same area...
Fair play, do u use a flow hood? I try to keep them separate due to heating problems and I wasnt trying to be a cock, just trying to be clear about the difference between the 2 areas.
Im also not a pro so keep it simple.
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: Monotub ready or not? [Re: plurfekt]
#23903127 - 12/07/16 12:06 PM (7 years, 1 month ago) |
|
|
Quote:
plurfekt said: Suppose you never considered my grow area and my work area, were in the same area...
Unless you grow in your flowhood/SAB, it actually isn't. The SAB might be in the same room, but if that room is draft free, it could be a room of garbage, still wouldn't affect your sterile work inside your SAB. Also what I find funny is that RR did flowhood work in his bedroom. I know a few others who have their flowhood in a pretty shabby room as well, so I still don't see how a rooms cleanliness is that important..
The thing that is needed beyond anything else is clean spawn. A clean grow area will allow you to grow more fruits using dirty spawn, but you can have a dirty area with clean spawn and see nothing affecting it.
https://www.shroomery.org/forums/showflat.php/Number/23746659#23746659
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 11 hours
|
Re: Monotub ready or not? [Re: Mad Season]
#23903131 - 12/07/16 12:08 PM (7 years, 1 month ago) |
|
|
Quote:
Mad Season said:
Quote:
plurfekt said: Suppose you never considered my grow area and my work area, were in the same area...
Unless you grow in your flowhood/SAB, it actually isn't. The SAB might be in the same room, but if that room is draft free, it could be a room of garbage, still wouldn't affect your sterile work inside your SAB. Also what I find funny is that RR did flowhood work in his bedroom. I know a few others who have their flowhood in a pretty shabby room as well, so I still don't see how a rooms cleanliness is that important..
https://www.shroomery.org/forums/showflat.php/Number/23746659#23746659
|
plurfekt
Finally Grateful

Registered: 12/14/09
Posts: 1,919
Loc: USA
|
Re: Monotub ready or not? [Re: Mad Season]
#23903225 - 12/07/16 12:41 PM (7 years, 1 month ago) |
|
|
Quote:
Mad Season said:
Quote:
plurfekt said: Suppose you never considered my grow area and my work area, were in the same area...
Unless you grow in your flowhood/SAB, it actually isn't. The SAB might be in the same room, but if that room is draft free, it could be a room of garbage, still wouldn't affect your sterile work inside your SAB. Also what I find funny is that RR did flowhood work in his bedroom. I know a few others who have their flowhood in a pretty shabby room as well, so I still don't see how a rooms cleanliness is that important..
The thing that is needed beyond anything else is clean spawn. A clean grow area will allow you to grow more fruits using dirty spawn, but you can have a dirty area with clean spawn and see nothing affecting it.
https://www.shroomery.org/forums/showflat.php/Number/23746659#23746659
Flowhood build put on hold; SAB for now. I would routinely rotate monotubs off for counter-space where I would do my sterile work, as I had a lot of tubs going and limited workspace. So, it was done in the same area, but I otherwise agree with what you are saying. I do occasionally perform inoculations and simple stuff in my bedroom, and I've yet to see a contamination from it, but I keep my bedroom freakishly* clean, too.
My experience when I was younger, was 100% success rates until someone decided to open a moldy jar full of trich and I watched a green POOF come out of it like a cloud and completely cover my work/grow area, and ever since then contamination was an absolute nightmare. I like keeping things so clean, I don't even use a SAB most of the time and can get away with open air inoculations routinely. It works for me, and makes it much easier to identify where contamination is coming from when it does occur, rather than just being like "whoop; more wasted grains/substrates due to the air, or whatever."
In the end it gives me peace of mind and saves money, I treat the work area as exactly that.
But like I said, too each their own - if you want to grow in a moldy old apartment, which I've also done with mostly success and higher contamination rates, have at it. I personally don't care for the idea. No one in this world would ever convince me it's better to grow in a mold-ridden room then a room as close to sterile as it can be.
Edited by plurfekt (12/07/16 12:45 PM)
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: Monotub ready or not? [Re: plurfekt]
#23903287 - 12/07/16 12:59 PM (7 years, 1 month ago) |
|
|
I can agree that getting contaminations and having an increased sporeload means more work, but it doesn't necessarily mean you'll always have more contaminations. If you're having lots of contaminations, it's your spawn, literally the hardest part of the hobby. I actually am so happy I have a moldy area. It made me notice morphologies of spawn that would tend to mold. If you look up my posts and type in bacteria, you can see how anal I am with my identifications.
But yes, definitely do what you want. A clean room is a very nice safety net. You can grow with some bacterial af shit, and still pull off fruits, whereas here I toss anything with even minor bacteria.
|
p3nisenvy
Stranger
Registered: 12/07/16
Posts: 1
Last seen: 7 years, 23 days
|
Re: Monotub ready or not? [Re: Mad Season]
#23903382 - 12/07/16 01:27 PM (7 years, 1 month ago) |
|
|
Sorry this is a little off topic but I'm on my way to doing a monotub but right now I have rye berries colonizing trying to find out if possibly I need to inoculate again because only one of my Rye grain jars are taking off after 5 days I know that's early but I'm curious was I supposed to use one CC's per quart jar with the Spore syringe or was it more than one cc conflicting info online because the the Jar thats taking off I think I used about one and a half if you could please let me know I'd appreciate it if you need to inbox me at or ill Chuck back here....neolutionevolution@gmail.com
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 11 hours
|
Re: Monotub ready or not? [Re: p3nisenvy]
#23903470 - 12/07/16 01:49 PM (7 years, 1 month ago) |
|
|
Patience, it can take 3 weeks sometimes.
Try making your own post instead of hijacking someone elses
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Monotub ready or not? [Re: Edmunter]
#23903481 - 12/07/16 01:52 PM (7 years, 1 month ago) |
|
|
Id say especially for a noob they just just toss shitty spawn...why start behind and risk future projects.
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
|
Re: Monotub ready or not? [Re: cronicr] 1
#23904013 - 12/07/16 03:47 PM (7 years, 1 month ago) |
|
|
Wait, are you Frank Edmunter? I just realized your avatar is the same.
|
plurfekt
Finally Grateful

Registered: 12/14/09
Posts: 1,919
Loc: USA
|
Re: Monotub ready or not? [Re: cronicr]
#23904336 - 12/07/16 05:18 PM (7 years, 1 month ago) |
|
|
Quote:
Mad Season said: I can agree that getting contaminations and having an increased sporeload means more work, but it doesn't necessarily mean you'll always have more contaminations. If you're having lots of contaminations, it's your spawn, literally the hardest part of the hobby. I actually am so happy I have a moldy area. It made me notice morphologies of spawn that would tend to mold. If you look up my posts and type in bacteria, you can see how anal I am with my identifications.
But yes, definitely do what you want. A clean room is a very nice safety net. You can grow with some bacterial af shit, and still pull off fruits, whereas here I toss anything with even minor bacteria.
Yea, once contamination sets into a grow area to a moderate-severe degree you see marked increase in contamination/losses, which you're quite right are often avoided with good practices; but when healthy cakes are catching trich, or you get cobweb/trich during the spawning to monotub process, it's like okay... Time to replace that HEPA filter again and bleach the floor/walls.
I've never had problems maintaining clean spawn, if anything I'm really lucky in that area - but I also agree seeing contamination/bacterial infection varieties is part of the learning process, I recall in my youth watching a half-colonized PF cake in the jar fight off a small trich contamination, which took a couple weeks, but then it fruited as normal. Very interesting to say the least.
I'm just OCD; and sometimes I am growing for others profit/benefit and can't take the risks that I would normally take on my own hobby projects, which is why I try to maintain such a high standard.
Generally for beginners I'd just tell them to get it right to understand sterile procedure and of course, over time they will naturally run into problems on their own.
And I fully support all aspiring mycologists to expand their hobby in whatever way they are inclined to do so.
There is not a one size fits all recipe for living, the shoe that fits one person pinches another. ~Carl Jung M.D.
Quote:
cronicr said: Id say especially for a noob they just just toss shitty spawn...why start behind and risk future projects.
Indeed, they'll save themselves some time/money/heartache.
|
Chips
Stranger

Registered: 01/05/16
Posts: 32
Last seen: 5 years, 3 months
|
Re: Monotub ready or not? [Re: plurfekt]
#23904419 - 12/07/16 05:46 PM (7 years, 1 month ago) |
|
|
Quote:
plurfekt said:
Quote:
Mad Season said: I can agree that getting contaminations and having an increased sporeload means more work, but it doesn't necessarily mean you'll always have more contaminations. If you're having lots of contaminations, it's your spawn, literally the hardest part of the hobby. I actually am so happy I have a moldy area. It made me notice morphologies of spawn that would tend to mold. If you look up my posts and type in bacteria, you can see how anal I am with my identifications.
But yes, definitely do what you want. A clean room is a very nice safety net. You can grow with some bacterial af shit, and still pull off fruits, whereas here I toss anything with even minor bacteria.
Yea, once contamination sets into a grow area to a moderate-severe degree you see marked increase in contamination/losses, which you're quite right are often avoided with good practices; but when healthy cakes are catching trich, or you get cobweb/trich during the spawning to monotub process, it's like okay... Time to replace that HEPA filter again and bleach the floor/walls.
I've never had problems maintaining clean spawn, if anything I'm really lucky in that area - but I also agree seeing contamination/bacterial infection varieties is part of the learning process, I recall in my youth watching a half-colonized PF cake in the jar fight off a small trich contamination, which took a couple weeks, but then it fruited as normal. Very interesting to say the least.
you do realize that contams can hide in spawn and sometimes you will have absolutely no idea they're there until you go to fruit and it contams right?
it just seems to me that you're in denial about not having problems maintaining clean spawn. especially when you're "getting away with" inoculating in open air, but then also getting cobweb/trich during the spawning to monotub process (hint: you're not actually getting away with it)
spawning is not sterile. if you're seeing contamination during the spawn run with clean spawn/substrate, it does not mean that your grow area is too moldy and spores got in during spawning and fucked everything up, it means the spawn you thought was clean was not actually clean
mad season made this very clear with his posts showing that he was growing just fine in a high sporeload environment
if you think that "healthy cakes catching trich" is because of your grow area you are kidding yourself. if the cake catches trich, it was not a healthy cake
please someone correct me if i'm wrong
|
plurfekt
Finally Grateful

Registered: 12/14/09
Posts: 1,919
Loc: USA
|
Re: Monotub ready or not? [Re: Chips]
#23904430 - 12/07/16 05:49 PM (7 years, 1 month ago) |
|
|
Thought someone may touch on this.
I'm well aware of it. But if my spawn is dirty, it's easy to figure that out, and toss it before using the master culture again.
Not saying that has never happened, I've been growing for a long time lol. I'm saying it's easier to determine, especially when I do my grows in sets from the same master cultures.
And I assure you, the air is never truly clean to a sterile degree, regardless.
This comes from experience, not an unfounded opinion.
|
Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
|
Re: Monotub ready or not? [Re: krypto2000]
#23904827 - 12/07/16 07:36 PM (7 years, 1 month ago) |
|
|
Quote:
krypto2000 said: Wait, are you Frank Edmunter? I just realized your avatar is the same.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
|
Chips
Stranger

Registered: 01/05/16
Posts: 32
Last seen: 5 years, 3 months
|
Re: Monotub ready or not? [Re: plurfekt]
#23904829 - 12/07/16 07:37 PM (7 years, 1 month ago) |
|
|
Quote:
plurfekt said: But if my spawn is dirty, it's easy to figure that out, and toss it before using the master culture again.
how do you figure it out?
Quote:
plurfekt said: This comes from experience, not an unfounded opinion.
it is possible to have experience and be misinterpreting what is going wrong (i know this from experience )
i'm just trying to figure out why you're so sure your spawn is clean while at the same time thinking contams are coming from your environment and not getting in during inoculation
i've been in the same boat, and then found out i was interpreting my experience completely wrong. if you know something i don't i'd like to learn lol
|
PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
|
Re: Monotub ready or not? [Re: Chips]
#23904903 - 12/07/16 07:51 PM (7 years, 1 month ago) |
|
|
Absolutely, contams during spawn run is because of bad spawn. You can to some degree catch bad spawn before tubbing it such as growth appearance, grain appearance, recovery speed/time from a shake, smell test, how easily spawn breaks up (very easily broken spawn and very hard to break spawn usually have off smells) but regardless of doing all of the above the spawn could still be bad from a slip up in the sterile technique. This can be easily found out by leaving fully colonized spawn jars sit on a shelf for several weeks, eventually whatever is hiding will show its face
|
plurfekt
Finally Grateful

Registered: 12/14/09
Posts: 1,919
Loc: USA
|
Re: Monotub ready or not? [Re: Chips]
#23904944 - 12/07/16 08:02 PM (7 years, 1 month ago) |
|
|
Simple process of elimination, correct sterile procedure, and redundancy.
Say I make two tubs with clean-ish looking spawn, both contaminate the same way, but I did an open air inoculation, or wasn't sure of the print source. It's fair to say it's the spawn, while not impossible it wasn't the air, over time the process of elimination suggests otherwise and I've seen it time and time again.
Solution: Redo the culture properly, and do not use it on anymore spawn/tubs/etc.
I also like to test LC's on pf-cakes if I am not sure they are clean, before I use the LC to inoculate spawn bags.
Works for me.
Good luck
|
PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
|
Re: Monotub ready or not? [Re: plurfekt]
#23905005 - 12/07/16 08:19 PM (7 years, 1 month ago) |
|
|
Uhhh...
|
PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
|
|
Dirty inoculant used to create spawn creates bad spawn, open air allows contams to enter spawn which creates bad spawn, poor sterile techniques create bad spawn <- all which lead to contaminated subs. Maybe I just don't understand what your trying to say 
|
plurfekt
Finally Grateful

Registered: 12/14/09
Posts: 1,919
Loc: USA
|
|
Quote:
PinPornProducer said: Dirty inoculant used to create spawn creates bad spawn, open air allows contams to enter spawn which creates bad spawn, poor sterile techniques create bad spawn <- all which lead to contaminated subs. Maybe I just don't understand what your trying to say  
My wording is fucked. I'm on a new benzo for a Xanax taper. I'll come back to this and explain in depth tomorrow.
One thing I will say drives me crazy, though, is when I do a single culture to a single spawn>tub and it contaminates, and never get to reuse the culture to test the theory.
Ideally this 'test' would be done on agar, of course. PF-Teks suffice in a pinch as you can just use a fat amount of LC, fast colonization, and it's not just spawn due to the rice content, set it to fruit (if it makes it that far) and wait and see...
I'm really speaking of the "hidden contaminants" in "clean looking spawn" that don't present easily. If it's dirty AF I'm going to notice that and toss it, obviously.
Process of elimination relates to using the same methods over and over (redundancy) and the patterns you find when you do this.
If that doesn't satisfy, I'll be around tomorrow with a clear head.
|
|