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PinPornProducer
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Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: Monotub ready or not? [Re: Chips]
#23904903 - 12/07/16 07:51 PM (7 years, 1 month ago) |
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Absolutely, contams during spawn run is because of bad spawn. You can to some degree catch bad spawn before tubbing it such as growth appearance, grain appearance, recovery speed/time from a shake, smell test, how easily spawn breaks up (very easily broken spawn and very hard to break spawn usually have off smells) but regardless of doing all of the above the spawn could still be bad from a slip up in the sterile technique. This can be easily found out by leaving fully colonized spawn jars sit on a shelf for several weeks, eventually whatever is hiding will show its face
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plurfekt
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Registered: 12/14/09
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Loc: USA
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Re: Monotub ready or not? [Re: Chips]
#23904944 - 12/07/16 08:02 PM (7 years, 1 month ago) |
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Simple process of elimination, correct sterile procedure, and redundancy.
Say I make two tubs with clean-ish looking spawn, both contaminate the same way, but I did an open air inoculation, or wasn't sure of the print source. It's fair to say it's the spawn, while not impossible it wasn't the air, over time the process of elimination suggests otherwise and I've seen it time and time again.
Solution: Redo the culture properly, and do not use it on anymore spawn/tubs/etc.
I also like to test LC's on pf-cakes if I am not sure they are clean, before I use the LC to inoculate spawn bags.
Works for me.
Good luck
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PinPornProducer
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Registered: 08/23/14
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Re: Monotub ready or not? [Re: plurfekt]
#23905005 - 12/07/16 08:19 PM (7 years, 1 month ago) |
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Uhhh...
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PinPornProducer
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Registered: 08/23/14
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Dirty inoculant used to create spawn creates bad spawn, open air allows contams to enter spawn which creates bad spawn, poor sterile techniques create bad spawn <- all which lead to contaminated subs. Maybe I just don't understand what your trying to say 
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plurfekt
Finally Grateful

Registered: 12/14/09
Posts: 1,919
Loc: USA
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Quote:
PinPornProducer said: Dirty inoculant used to create spawn creates bad spawn, open air allows contams to enter spawn which creates bad spawn, poor sterile techniques create bad spawn <- all which lead to contaminated subs. Maybe I just don't understand what your trying to say  
My wording is fucked. I'm on a new benzo for a Xanax taper. I'll come back to this and explain in depth tomorrow.
One thing I will say drives me crazy, though, is when I do a single culture to a single spawn>tub and it contaminates, and never get to reuse the culture to test the theory.
Ideally this 'test' would be done on agar, of course. PF-Teks suffice in a pinch as you can just use a fat amount of LC, fast colonization, and it's not just spawn due to the rice content, set it to fruit (if it makes it that far) and wait and see...
I'm really speaking of the "hidden contaminants" in "clean looking spawn" that don't present easily. If it's dirty AF I'm going to notice that and toss it, obviously.
Process of elimination relates to using the same methods over and over (redundancy) and the patterns you find when you do this.
If that doesn't satisfy, I'll be around tomorrow with a clear head.
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