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OfflineSpiggle
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How is the mycelium?
    #23880671 - 11/30/16 04:52 AM (7 years, 1 month ago)

Hey
I've been working with a strain of Psilocybe cubensis, after a few transfers i had nice rizomorphic mycelium nothing amazing but it looked to be healthy and thick, i took sectors from these rizomorphic spots but now the mycelium has taken on a cottony appearance, and it looks very uniform.. is the mycelium senscing because of too many transfers?
I know very little about growing, feel free to correct me anywhere!
First picture is the healthy mycelium, the others are the product of transfers from the first.



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OfflinecronicrFacebook
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Re: How is the mycelium? [Re: Spiggle]
    #23880676 - 11/30/16 04:55 AM (7 years, 1 month ago)

No its not too much transfers try switch your media


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OfflineAlCapone2k
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Re: How is the mycelium? [Re: cronicr]
    #23880730 - 11/30/16 05:55 AM (7 years, 1 month ago)

The pictures aren't that good due to condensation but what I can see, your myc is fine for inoculating. If you want to be sure, make one or two further transfers but IMO you are fine to go for inoculation.

You have a nice evenly growth, don't overthink that thing with rhizo or tomentose growth. It's more about nutritions and other circumstances on your agar plate. A tomentose myc can grow rhizo once it's used for inoculating a jar.


Have fun! :smile:


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OfflineSpiggle
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Re: How is the mycelium? [Re: AlCapone2k]
    #23880942 - 11/30/16 08:34 AM (7 years, 1 month ago)

Yeah sorry about the picture quality! is there a way to not get condensation on the plates?
Thanks for the replies, its cool it isn't to do with the amount of transfers but rather the media.. really interesting stuff! will be using these for grains now :smile:


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Edited by Spiggle (11/30/16 08:36 AM)


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OfflineAlCapone2k
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Re: How is the mycelium? [Re: Spiggle]
    #23880998 - 11/30/16 09:03 AM (7 years, 1 month ago)

To prevent condensation you should minimize temperature fluctuation as much as possible. You should store them at the temperature the room had when pouring them.

If you have to change the temperature (e.g. bc of putting them into the refrigerator) you should put them in an insulated lunch bag or styrofoam box or something.


Regards


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Since I am from Germany, my english is not perfect but I try my best :smile:




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Invisibler.lutece
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Re: How is the mycelium? [Re: AlCapone2k]
    #23881032 - 11/30/16 09:21 AM (7 years, 1 month ago)

In addition to storing them in an insulated container, allowing your agar to solidify after pouring with the petris stacked in a column will help prevent condensation.


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OfflineAlCapone2k
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Re: How is the mycelium? [Re: r.lutece]
    #23881056 - 11/30/16 09:34 AM (7 years, 1 month ago)

Yes, what he said. Pouring in stacks of 10 is fine, gives you 8 good plates and two with a bit of condensation. You can get rid of this condensation in these two plates by putting a metal cup with hot (not boiling) water on them.

Regards


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Since I am from Germany, my english is not perfect but I try my best :smile:




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InvisibleJacobStorm
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Re: How is the mycelium? [Re: AlCapone2k]
    #23881071 - 11/30/16 09:39 AM (7 years, 1 month ago)

Quote:

AlCapone2k said:
Yes, what he said. Pouring in stacks of 10 is fine, gives you 8 good plates and two with a bit of condensation. You can get rid of this condensation in these two plates by putting a metal cup with hot (not boiling) water on them.

Regards




Seriously?  Wow cool didn't know that :takingnotes:


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OfflineAlCapone2k
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Re: How is the mycelium? [Re: JacobStorm]
    #23881094 - 11/30/16 09:50 AM (7 years, 1 month ago)

Yeah, pour from bottom to top plate. Watch YouTube how to do it


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Since I am from Germany, my english is not perfect but I try my best :smile:




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Invisibler.lutece
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Re: How is the mycelium? [Re: JacobStorm]
    #23881156 - 11/30/16 10:30 AM (7 years, 1 month ago)

Quote:

JacobStorm said:
Quote:

AlCapone2k said:
Yes, what he said. Pouring in stacks of 10 is fine, gives you 8 good plates and two with a bit of condensation. You can get rid of this condensation in these two plates by putting a metal cup with hot (not boiling) water on them.

Regards




Seriously?  Wow cool didn't know that :takingnotes:




RR's Let's Grow Mushrooms series in particular is incredible if you haven't already seen it. He details pouring a stack of agar plates better than probably anyone else I've seen.


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InvisibleJacobStorm
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Re: How is the mycelium? [Re: AlCapone2k]
    #23881166 - 11/30/16 10:33 AM (7 years, 1 month ago)

I have the videos. He never mentioned

Quote:

AlCapone2k said: You can get rid of this condensation in these two plates by putting a metal cup with hot (not boiling) water on them.




Which is a useful nugget of info.


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Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


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InvisibleMad Season
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Re: How is the mycelium? [Re: JacobStorm]
    #23881291 - 11/30/16 11:20 AM (7 years, 1 month ago)

Weird that he doesn't mention it in the videos. I've seen him say it on here tons of times to stack them and put a warm coffee mug on top. Personally I just stack them, and if there's condensation leftover :shrug: a little extra water hasn't ever stopped me before.

OP your plates look alright to me. They fuzz up when there's different nutrient ratios in your agar. If ya took away some of the nutrients, i bet it'll rizmorph again.


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OfflineAlCapone2k
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Re: How is the mycelium? [Re: Mad Season]
    #23881359 - 11/30/16 11:48 AM (7 years, 1 month ago)

Yeah, I also don't care anymore about the condensation on the top two plates. I have recognized that it disappears after a while anyway when stored in a Ziploc Bag.


Regards.


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Since I am from Germany, my english is not perfect but I try my best :smile:




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OfflinecronicrFacebook
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Re: How is the mycelium? [Re: Mad Season]
    #23881386 - 11/30/16 11:57 AM (7 years, 1 month ago)

Quote:

Mad Season said:
Weird that he doesn't mention it in the videos. I've seen him say it on here tons of times to stack them and put a warm coffee mug on top. Personally I just stack them, and if there's condensation leftover :shrug: a little extra water hasn't ever stopped me before.

OP your plates look alright to me. They fuzz up when there's different nutrient ratios in your agar. If ya took away some of the nutrients, i bet it'll rizmorph again.



The stacking after pouring is the same concept the hot water thing is more for after the fact....op your cultures are probably getting lazy or you're not taking the leading edge.


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OfflineSpiggle
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Re: How is the mycelium? [Re: cronicr]
    #23883571 - 12/01/16 03:09 AM (7 years, 1 month ago)

Hey cronicr, how would i go about removing nutrients from my original recipe? i use the standers PDA technique... it will go back to rhizo good news :smile: !


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OfflinecronicrFacebook
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Re: How is the mycelium? [Re: Spiggle]
    #23883581 - 12/01/16 03:21 AM (7 years, 1 month ago)

If you used pda the whole time try mea or just anything different


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It doesn't matter what i think of you...all that matters is clean spawn

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OfflineSpiggle
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Re: How is the mycelium? [Re: cronicr]
    #23889746 - 12/03/16 06:22 AM (7 years, 1 month ago)

Thank you! i'm going to try mea


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OfflineSpiggle
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Re: How is the mycelium? [Re: Spiggle]
    #23920651 - 12/12/16 06:48 PM (7 years, 1 month ago)

Hey guys thought I'd post a pic of the culture after keeping it in the fridge for a couple weeks, growth has stopped and a really beautiful ring formed thought it was cool here it is:



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OfflinecronicrFacebook
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Re: How is the mycelium? [Re: Spiggle]
    #23920666 - 12/12/16 06:52 PM (7 years, 1 month ago)

Quote:

Spiggle said:
Hey guys thought I'd post a pic of the culture after keeping it in the fridge for a couple weeks, growth has stopped and a really beautiful ring formed thought it was cool here it is:





:mindblown:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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