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frankwhite85
Stranger


Registered: 04/18/16
Posts: 305
Last seen: 1 month, 6 days
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pasty agar tek preference
#23880638 - 11/30/16 04:22 AM (7 years, 1 month ago) |
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So I know Pasty agar is generally for people using SAB's but what about if you have a flowhood? It seems like if its done correctly it leaves less to chance than pouring petris in front of a hood even if the air is 99.99% sterile. And I like the idea of a tiger drop instead of having to fish the wedge out with a scalpel to the grain jar.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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You certainly can
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: pasty agar tek preference [Re: cronicr]
#23880736 - 11/30/16 05:58 AM (7 years, 1 month ago) |
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I never did pastys agar tek, but I can't imagine why it shouldn't be able to use it with a FH.
Furthermore you can do tiger drop (Version 1 or Tic Tac to) with a petri dish too. Why not, just cut the tic tac to pattern in your agar petri and drop it into the jar.
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: pasty agar tek preference [Re: AlCapone2k]
#23880813 - 11/30/16 07:17 AM (7 years, 1 month ago) |
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I don't think I would be able to squeeze the petri to drop the agar. The tiger drop method can only be done with a container that is a) flexible and b) smaller in diameter than the recieving jars mouth. It's one of the reasons that I like the glad containers so much, few others have those attributes.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: pasty agar tek preference [Re: Pastywhyte]
#23880833 - 11/30/16 07:26 AM (7 years, 1 month ago) |
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Yeah, I did it some times a bit modified, without squeezing, when I want a whole plate to be dropped in the jar.
I cut a circle around the myc part and lift it a bit with the scalpel that it's losen from the petri dish. Then I drop it into the jar.
Sorry for the misunderstanding, it's not totally the same like your tiger drop but somekind of
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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frankwhite85
Stranger


Registered: 04/18/16
Posts: 305
Last seen: 1 month, 6 days
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Re: pasty agar tek preference [Re: AlCapone2k]
#23881570 - 11/30/16 12:59 PM (7 years, 1 month ago) |
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I could definitely imagine doing the drop if you loosened it up with a scalpel first, with a flowhood you'd definately have to drop it from behind the jar quickly so you don't blow in any contams that might be on the petri.
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Quote:
frankwhite85 said: with a flowhood you'd definately have to drop it from behind the jar quickly so you don't blow in any contams that might be on the petri.
That's true. I already did this with bags sometimes. Cut a circle and a cross on the petri to have 4 quarters. Place it behind the bag/jar use the scalpel to drop.
I usually use jars and lift about a quarter of a dish with the scalpel to inoculate.
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