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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Annoying SAB work ... what do TCs do?
#23877509 - 11/29/16 08:28 AM (7 years, 2 months ago) |
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Hey guys,
I am a bit annoyed of SAB work but I can't afford a flow hood. Since I live in a small appartement for students I don't have an extra table for my SAB so every time I want to do sterile work, I have to ...
1. put a laundried towel on the table 2. spray it with soap water 3. put everything on the towel that I need 4. spray the walls and the roof of my SAB mit Soap water 5. put the SAB upside down on the towel 6. take a shower 7. wear a laundried long sleeve 8. gloves, mask, hairnet and that stuff.
This in most cases take at least 20 minutes. This is really anoying. Especially I'm ranning out of towels cause at the moment every two days I have to do sterile work.
How do you guys do that?
1. Do you also change the towel every time you doing SAB work? Or is it possible to use one towel a couple of times and just spray it with new soap water every SAB work?
2. Is it necessary that I always wear a freshly laundried long sleeve or can I reuse it some times?
3. I always see pictures of these great "SAB-Tables" where the SAB seems to stay the whole time. How do you prepare for SAB work? Do you change the towel every time, spray it with soapy water and spray the walls with soapy water? Or is once in a week enough? I sometimes read that people pouring agar plates and without sealing them with parafilm/saran wrap they let them rest in their SAB until they inoculate them. How do these guys do that? I thought spraying soapy water is essential every sterile work session?
Would be really nice to have some kind of "This is how I procede when doing SAB sterile work"-Thread with some TCs telling how they do it 
I hope my questions are understandable. I have a very low contamn rate, especially with agar it is under 5% I guess. All my transfers work well and so on. But it takes really a lot of time to prepare everything for sterile work. I want to "save some time", but actually I don't want to change anything that could ruin my rate.
This is my SAB by the way:

Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Enigma1
Positive



Registered: 08/15/13
Posts: 977
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#23877553 - 11/29/16 08:44 AM (7 years, 2 months ago) |
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I'm no TC be patience you are doing a good .
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#23877560 - 11/29/16 08:46 AM (7 years, 2 months ago) |
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SpitballJedi's thread on how he does his SAB work is probably exactly what you're looking for. He details pretty much the exact process you've described. You are using correct sterile procedure to set up your SAB, yes. As to your questions:
1. I'd change that towel out every time. Damp towels grow molds. That's why they start to smell mildewy.
2. If you remove that long sleeve after you're done and put it in a grocery bag until next time, you could probably reuse it. Basically, if it's only worn during SAB work, you can get a few uses from it before washing it.
3. If you have a dedicated work station, you can leave your SAB in place, but it doesn't work for the damp towel method. If you were using the tub with the lid and got rid of the towel, that might be another story, but I'd still set it up fresh each time. Agar plates can be left in an SAB to gel up not because it is sterile (it's not), but it's still air: there shouldn't be many, if any, contams floating around. So it's pretty safe to leave dishes in there. However, the damp towel presents a germination point for any spores that drift in, even if it's only a few. So it's best not to reuse that towel for the next run.
SAB work is just one of those things that will always take a decent amount of time to perform, especially in an SAB. A flow hood would speed things up for you, but I know those aren't an option for everyone. Youre doing great, it just takes patience. That SAB of yours is awesome by the way. That tub is one of the most perfect ones I've seen.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Annoying SAB work ... what do TCs do? [Re: r.lutece]
#23877601 - 11/29/16 08:56 AM (7 years, 2 months ago) |
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Thank you very much for your kind answer buddy and thanks for the compliment to my SAB 
Yeah, it really takes some time to prepare everything and it's worth it. As I mentioned my contamn rate (on agar plates, unfortunately not that good on rye jars) is very good and therefore my sterile technique seems to be good aswell. But I thought there could me some hints to save some time, you know
The towel thing is something not that annoying, but I am running out of towels. Sometimes, when I have a lot of projects at the same time (oyser, cubes, agar, jars etc.) I have to do SAB work every day for a week or so. 7 towels in one week is a bit to much for a bachelor appartement :P
And pasty's SAB tek is the one I read before I ever started with SAB work and since then I am doing it the way he does.
Regards from Germany.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (11/29/16 08:58 AM)
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
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Re: Annoying SAB work ... what do TCs do? [Re: r.lutece]
#23877602 - 11/29/16 08:58 AM (7 years, 2 months ago) |
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Ohhhh the trials and tribulations of growing mushrooms.....
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Annoying SAB work ... what do TCs do? [Re: Peteyboy]
#23877627 - 11/29/16 09:07 AM (7 years, 2 months ago) |
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Tell me about it!
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Annoying SAB work ... what do TCs do? [Re: Peteyboy]
#23877641 - 11/29/16 09:11 AM (7 years, 2 months ago) |
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try to plan ahead and consolidate ur work into just one or two sessions per week.
most plates and jars seem to work well within a 7-10 day(or 14-20) timeline.
it might take a couple weeks, but chances are you will fall into a personal routine, so that maybe every tuesday ur plates are at 75% (because u nocced them up 10 days ago), and you noc up masters. 14 days later, u can g2g them all, etc. this really helps keeps things organized for me. obviously, with liquids, your timeline would be different, but the inoculation pattern would be similar.
as far as what u can cut out of ur procedure to save time, I would say just let it ride as is. most of the "short cuts" I take, I never planned on them. it just happened that one day I was short on time, so skipped X or Y, and everything still went fine, so from then on, I stopped doing whatever that was. after a few months, you will inevitably find out the quickest and easiest and safest way for you to do you work just by trial, error and observation.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Annoying SAB work ... what do TCs do? [Re: blindingleaf]
#23877667 - 11/29/16 09:22 AM (7 years, 2 months ago) |
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Thank you blindingleaf for your answer.
It's good to hear that from a TC, so I go on with my procedure
The problem in consolidating work is that my SAB get's very "full of stuff" when doing more than one thing once.
Is it absolutely ok to let's say first pour agar plates, get out of the SAB and let them dry for 20 minutes, then get back, put them into a Ziploc and take the ziploc out of it and then without spraying or doing something else get the jars into the SAB and go on with jar work? Or will I bring to many contaminants into my SAB when changing the exquipment inside it?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#23877697 - 11/29/16 09:36 AM (7 years, 2 months ago) |
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When I do multiple tasks in one day...I break it up...for example...I'll do my agar transfers andbany swiping i need to do first, then when finished I take the lid off my SAB remove the items,then i wipe down and put my grain jars in and dishes I'm dropping, then i respray replace the lid and wait another 30 min then go back to work...might take longer but leaves you plenty of room to work and minimizes mistakes from clutter. And provides an excellent opportunity for a midsession blunt...
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Annoying SAB work ... what do TCs do? [Re: Peteyboy]
#23877714 - 11/29/16 09:43 AM (7 years, 2 months ago) |
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i think petes way sounds like a good bet.
theres a lot of ways that people do things though. some people don't use gloves, some don't use iso, some spray iso in the box, some outside of it, etc
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Annoying SAB work ... what do TCs do? [Re: blindingleaf]
#23877723 - 11/29/16 09:49 AM (7 years, 2 months ago) |
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You don't need to take a shower before work with a SAB However if you had a FH it's more important to take a shower before hand
You should let a FH run at least an hour before working but you can use a SAB nearly immediately
I use no hairnet or mask. Never have. I also only use gloves sometimes.
It's far more important to use gloves, masks, and hairnets with a hood since it uses moving air.
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
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Re: Annoying SAB work ... what do TCs do? [Re: blindingleaf]
#23877727 - 11/29/16 09:51 AM (7 years, 2 months ago) |
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Thanks BL! That just made my day ! Lol
But yeah there's def more then one way to skin a cat haha
Having lit myself on fire once already in this hobby and suffering pretty serious 2nd degree burns, I would recommend keeping the iso to a minimum and far away from your torch haha.
I keep one glass dish with a paper towel outside and one inside the SAB. Both with lids I keep the lid on the dish outside the SAB since I do all my flaming outside the box. The dish inside loses its lid once I begin.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Annoying SAB work ... what do TCs do? [Re: Peteyboy]
#23877737 - 11/29/16 09:57 AM (7 years, 2 months ago) |
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Your tips sound good guys
Thank you bodhi, to avoid the shower step saves a lot of time (and money), because sometimes I showered three times a day because I had to do three times sterile work. That is not only not good for the money, it's also bad for your skin.
So in future I don't take a shower.
But I think I will go on wearing gloves because I am a bit nooby in my movings sometimes and withouth gloves it's a bit dangerous, especially over open media.
What do you guys think about my Long Sleeve problem? Is it ok to use it several times before laundry when I only use it for SAB work?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#23877748 - 11/29/16 10:02 AM (7 years, 2 months ago) |
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Meh. SAB work is kind of a pain in the ass, but it's part of it.
I about what you do TS but skip the showe. Fuck it. I put bleach water on my arms and gloves. Keep a small ISO wipe inside for your jars/plates. I've also caught myself on fire via ISO. So don't overdo it.
-------------------- KSSS And PE WBS.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Annoying SAB work ... what do TCs do? [Re: Moabfighter]
#23877753 - 11/29/16 10:03 AM (7 years, 2 months ago) |
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bleach on ur skin is hardcore dude! that would bother my nose and throat too much breathing it while working
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Annoying SAB work ... what do TCs do? [Re: blindingleaf]
#23877766 - 11/29/16 10:07 AM (7 years, 2 months ago) |
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Quote:
blindingleaf said: bleach on ur skin is hardcore dude! that would bother my nose and throat too much breathing it while working
Fuck it. Something I'm doing is working as I've never had a satellite or any molds.
-------------------- KSSS And PE WBS.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Annoying SAB work ... what do TCs do? [Re: Moabfighter]
#23877779 - 11/29/16 10:13 AM (7 years, 2 months ago) |
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I don't use bleach at all, actually I don't even exactly know what it is and Google can't help me. It seems to be some sanitizer or something.
I only use Iso and not that much. Just enough to wash my gloves and with a towel of Iso i wipe down my jars and dishes and so one. Not more
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Annoying SAB work ... what do TCs do? [Re: Moabfighter]
#23877789 - 11/29/16 10:15 AM (7 years, 2 months ago) |
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I always wear short sleeve shirts. Long sleeves seem counterproductive to me. If you want sleeves use tyvek so there's no lint
Bleach is sodium hypochlorite Or chlorox is name brand probably even in Germany
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#23877813 - 11/29/16 10:21 AM (7 years, 2 months ago) |
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I'm pretty sure bleach is sodium hypochlorite....and it contains chlorine which is highly toxic....when mixed with an acidic substance it will release the chlorine molecule in its gaseous state...death by breath!
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Annoying SAB work ... what do TCs do? [Re: bodhisatta]
#23877834 - 11/29/16 10:26 AM (7 years, 2 months ago) |
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Ah, ok, Sodiumhypochlorite I know. It's a really bad stuff. My father worked several years in a bakery at the cleaning station with this stuff and now he suffers from bad asthma. Physicians weren't sure for the reason but they said that it's probably the shc.
But of course we don't use it as much as they used it in the bakery.
as I said, I only use Iso, it works fine for me. Even the bottle of desinfectant that I bought I don't use at all anymore.
Quote:
I always wear short sleeve shirts. Long sleeves seem counterproductive to me. If you want sleeves use tyvek so there's no lint
Yeah, I have read that the skin is the No. 1 contamn factor?! So I bought two cheap longsleeves that I wear and I stuff the sleeves into the gloves so now skin is uncovered inside the SAB.
I also tryed tyvek sleeves but they are not washable and after a while I start to sweat in tyvek, so when using it couple of times, I think it's a contamn risk.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (11/29/16 10:27 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#23877849 - 11/29/16 10:30 AM (7 years, 2 months ago) |
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Hold a flashlight up and shake a shirt sleeve in the beam of the light. Then rub your arm. See which one releases far more particles
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#23877853 - 11/29/16 10:30 AM (7 years, 2 months ago) |
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I usually don't even wear a shirt, if you wipe your forearms down with iso when you wipe your hands your good! Helps keep you cool!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Annoying SAB work ... what do TCs do? [Re: Peteyboy]
#23877865 - 11/29/16 10:33 AM (7 years, 2 months ago) |
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Use a little bit of lotion or petroleum jelly on your arms and hands to keep the shedding of particles to a minimum
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Annoying SAB work ... what do TCs do? [Re: bodhisatta]
#23877866 - 11/29/16 10:34 AM (7 years, 2 months ago) |
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Quote:
bodhisatta said: Hold a flashlight up and shake a shirt sleeve in the beam of the light. Then rub your arm. See which one releases far more particles
Sounds clear, I think you are right. Especially after using a sleeve more than once, it will hold some contaminants I guess.
Next time I will wash my arm with soapy water and see, if it's enough 
Quote:
bodhisatta said: Use a little bit of lotion or petroleum jelly on your arms and hands to keep the shedding of particles to a minimum
This is a very good tip, thanks bodhi
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (11/29/16 10:34 AM)
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Annoying SAB work ... what do TCs do? [Re: bodhisatta]
#23877964 - 11/29/16 11:12 AM (7 years, 2 months ago) |
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Quote:
bodhisatta said: Use a little bit of lotion or petroleum jelly on your arms and hands to keep the shedding of particles to a minimum

I do some what the same, but i just soap my whole arm, and keep it wet, keeping it wet tends to reduce shedding too.
And Good idea Bodhisatta, don't know why i never thought of that. 
I've never cleaned my SAB, its literally a glass fish aquarium (open part is enclosed with plastic, my arms go through that)
And so far, i've never got a contam in any of my agar work, especially since i spray them down with 10% bleach water before working. (actually i have, but were mostly due to stupid mistakes lol, and mostly due to not spraying my SAB before working)
All the contams just settle down on the towel below.
I've honestly never changed my towel, i mean NEVER lol, the only time i get contams is when i haven't sprayed down the SAB before working in it.
This is because when you wet down the SAB what ever that collects in the towel stays in the towel, unless you physically transfer it.
This is IME so some people might not agree to this.
Your movement in the SAB is most important, especially how you handle agar inside (i recommend you to go through Mad Seasons thread HERE it really helped me with agar work in a SAB) after a while you just get use to the flow and will be doing things smoothly and swiftly.
Edited by Teemo 6T3 (11/29/16 11:15 AM)
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Annoying SAB work ... what do TCs do? [Re: Teemo 6T3]
#23877980 - 11/29/16 11:22 AM (7 years, 2 months ago) |
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I added more bleach to my water before this last go today and man.... too much bleach is no good. Dilute the hell out of that shit
-------------------- KSSS And PE WBS.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Annoying SAB work ... what do TCs do? [Re: Moabfighter]
#23878007 - 11/29/16 11:35 AM (7 years, 2 months ago) |
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You don't need to use a lot of bleach, Soap water will work just the same too.
The goal for all of this is to make whatever is floating inside the SAB to just settle down and stay put.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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synthetik
biological entity

Registered: 12/15/16
Posts: 194
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#24016175 - 01/16/17 11:04 PM (7 years, 14 days ago) |
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ya gotta love running around your city looking for random shit pertaining to this hobby, some days its a sketchy asian grocery for agar or off to the vape store for nicrome/kanthal and syringes... well today I hit walmart target home deopt and lowes in search of one of these nice tall clear tubs in the 100qt+ range... nope... good news I didnt shoot anyone in traffic so thats good
where are you guys finding these things? im to the point of making something either out of plexi or clear flexible vinyl, any ides?
edit: found this... just need a table top version

Edited by synthetik (01/16/17 11:44 PM)
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#24016241 - 01/16/17 11:34 PM (7 years, 14 days ago) |
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Quote:
AlCapone2k said: The problem in consolidating work is that my SAB get's very "full of stuff" when doing more than one thing once.
I try and tetris things together. So if 6 pasties and 4 pp5's and jars doesn't fit, I adjust accordingly. Try to plan out how you're working. Do sessions. Take a moment away, spray, set, get back to work. It is much better than having too little space (or too much). Maximize the use of every square inch of your SAB and PC.
A big SAB is nice but if you don't have the room, plan it out as to where you make the fewest movements and your tools won't whack into things. It sounds as though you're comfortably working? Don't change a thing just make sure rings and watches are off.
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Intelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
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Re: Annoying SAB work ... what do TCs do? [Re: Adden]
#24016292 - 01/17/17 12:00 AM (7 years, 14 days ago) |
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I feel I'm super sketchy with my sterile technique but I rarely get contams. I'm no TC but I think once you get the hang of sterile work it becomes simple.
I usually just change into a clean shirt, no shower. Put on gloves, mask, whipe my gloves first with an Iso paper towel, then I wipe both arms with it.
I use a cardboard sheet with black 3m plastic on it to set my SAB on. Usually I keep my SAB wrapped over closed so half the time I don't wipe the insides. Just do a couple sprays of a light soap water/bleach solution. I like to do a few sprays outside close to my shirt/arms as well at this point.
Takes me approximately 5-7mins to prep myself and all my tools before I enter. I give the room I'm working with about 10mins or so with the SAB sitting in it before I enter as well.
Last contam was 12/17/16 with the notes I made of - bacterial, poor transfer - (lol just realized its exactly 1 month since my last contam )
If Im in my SAB for more than 15mins I do put on tvek sleeves and tuck into my gloves. (thats rare though)
lastly If you make LC's with SHIPS like I do you can do open air injections and only have to mess with SAB's with agar and LC's. No g2g ever. ...some might say don't do this but fugem
Edited by Intelligentxfruit (01/17/17 03:56 AM)
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