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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#23877849 - 11/29/16 10:30 AM (7 years, 2 months ago) |
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Hold a flashlight up and shake a shirt sleeve in the beam of the light. Then rub your arm. See which one releases far more particles
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#23877853 - 11/29/16 10:30 AM (7 years, 2 months ago) |
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I usually don't even wear a shirt, if you wipe your forearms down with iso when you wipe your hands your good! Helps keep you cool!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Annoying SAB work ... what do TCs do? [Re: Peteyboy]
#23877865 - 11/29/16 10:33 AM (7 years, 2 months ago) |
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Use a little bit of lotion or petroleum jelly on your arms and hands to keep the shedding of particles to a minimum
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Annoying SAB work ... what do TCs do? [Re: bodhisatta]
#23877866 - 11/29/16 10:34 AM (7 years, 2 months ago) |
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Quote:
bodhisatta said: Hold a flashlight up and shake a shirt sleeve in the beam of the light. Then rub your arm. See which one releases far more particles
Sounds clear, I think you are right. Especially after using a sleeve more than once, it will hold some contaminants I guess.
Next time I will wash my arm with soapy water and see, if it's enough 
Quote:
bodhisatta said: Use a little bit of lotion or petroleum jelly on your arms and hands to keep the shedding of particles to a minimum
This is a very good tip, thanks bodhi
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (11/29/16 10:34 AM)
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Annoying SAB work ... what do TCs do? [Re: bodhisatta]
#23877964 - 11/29/16 11:12 AM (7 years, 2 months ago) |
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Quote:
bodhisatta said: Use a little bit of lotion or petroleum jelly on your arms and hands to keep the shedding of particles to a minimum

I do some what the same, but i just soap my whole arm, and keep it wet, keeping it wet tends to reduce shedding too.
And Good idea Bodhisatta, don't know why i never thought of that. 
I've never cleaned my SAB, its literally a glass fish aquarium (open part is enclosed with plastic, my arms go through that)
And so far, i've never got a contam in any of my agar work, especially since i spray them down with 10% bleach water before working. (actually i have, but were mostly due to stupid mistakes lol, and mostly due to not spraying my SAB before working)
All the contams just settle down on the towel below.
I've honestly never changed my towel, i mean NEVER lol, the only time i get contams is when i haven't sprayed down the SAB before working in it.
This is because when you wet down the SAB what ever that collects in the towel stays in the towel, unless you physically transfer it.
This is IME so some people might not agree to this.
Your movement in the SAB is most important, especially how you handle agar inside (i recommend you to go through Mad Seasons thread HERE it really helped me with agar work in a SAB) after a while you just get use to the flow and will be doing things smoothly and swiftly.
Edited by Teemo 6T3 (11/29/16 11:15 AM)
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Annoying SAB work ... what do TCs do? [Re: Teemo 6T3]
#23877980 - 11/29/16 11:22 AM (7 years, 2 months ago) |
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I added more bleach to my water before this last go today and man.... too much bleach is no good. Dilute the hell out of that shit
-------------------- KSSS And PE WBS.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Annoying SAB work ... what do TCs do? [Re: Moabfighter]
#23878007 - 11/29/16 11:35 AM (7 years, 2 months ago) |
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You don't need to use a lot of bleach, Soap water will work just the same too.
The goal for all of this is to make whatever is floating inside the SAB to just settle down and stay put.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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synthetik
biological entity

Registered: 12/15/16
Posts: 194
Last seen: 3 years, 10 months
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#24016175 - 01/16/17 11:04 PM (7 years, 14 days ago) |
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ya gotta love running around your city looking for random shit pertaining to this hobby, some days its a sketchy asian grocery for agar or off to the vape store for nicrome/kanthal and syringes... well today I hit walmart target home deopt and lowes in search of one of these nice tall clear tubs in the 100qt+ range... nope... good news I didnt shoot anyone in traffic so thats good
where are you guys finding these things? im to the point of making something either out of plexi or clear flexible vinyl, any ides?
edit: found this... just need a table top version

Edited by synthetik (01/16/17 11:44 PM)
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Annoying SAB work ... what do TCs do? [Re: AlCapone2k]
#24016241 - 01/16/17 11:34 PM (7 years, 13 days ago) |
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Quote:
AlCapone2k said: The problem in consolidating work is that my SAB get's very "full of stuff" when doing more than one thing once.
I try and tetris things together. So if 6 pasties and 4 pp5's and jars doesn't fit, I adjust accordingly. Try to plan out how you're working. Do sessions. Take a moment away, spray, set, get back to work. It is much better than having too little space (or too much). Maximize the use of every square inch of your SAB and PC.
A big SAB is nice but if you don't have the room, plan it out as to where you make the fewest movements and your tools won't whack into things. It sounds as though you're comfortably working? Don't change a thing just make sure rings and watches are off.
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Intelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
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Re: Annoying SAB work ... what do TCs do? [Re: Adden]
#24016292 - 01/17/17 12:00 AM (7 years, 13 days ago) |
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I feel I'm super sketchy with my sterile technique but I rarely get contams. I'm no TC but I think once you get the hang of sterile work it becomes simple.
I usually just change into a clean shirt, no shower. Put on gloves, mask, whipe my gloves first with an Iso paper towel, then I wipe both arms with it.
I use a cardboard sheet with black 3m plastic on it to set my SAB on. Usually I keep my SAB wrapped over closed so half the time I don't wipe the insides. Just do a couple sprays of a light soap water/bleach solution. I like to do a few sprays outside close to my shirt/arms as well at this point.
Takes me approximately 5-7mins to prep myself and all my tools before I enter. I give the room I'm working with about 10mins or so with the SAB sitting in it before I enter as well.
Last contam was 12/17/16 with the notes I made of - bacterial, poor transfer - (lol just realized its exactly 1 month since my last contam )
If Im in my SAB for more than 15mins I do put on tvek sleeves and tuck into my gloves. (thats rare though)
lastly If you make LC's with SHIPS like I do you can do open air injections and only have to mess with SAB's with agar and LC's. No g2g ever. ...some might say don't do this but fugem
Edited by Intelligentxfruit (01/17/17 03:56 AM)
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