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snawwz
Beginner Cultivator


Registered: 11/07/16
Posts: 139
Last seen: 6 years, 10 months
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What agar sectors should be isolated
#23875731 - 11/28/16 05:04 PM (7 years, 2 months ago) |
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Hi!
I'm a new grower, and I'm looking for some guidance on what I should isolate.
These Petri dishes have been isolated once so far from MS syringe. They were isolated from a single Petri dish.
The center might look a little strange cause there is an extra chunk of agar from the previous dishes.
Dish #1:
Dish #2:
Dish #3:
-------------------- snawwz ✌
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snawwz
Beginner Cultivator


Registered: 11/07/16
Posts: 139
Last seen: 6 years, 10 months
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Re: What agar sectors should be isolated [Re: snawwz]
#23875735 - 11/28/16 05:06 PM (7 years, 2 months ago) |
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Also, how do you guys think the isolations are going so far? How many more do you think will be needed?
Thanks!
-------------------- snawwz ✌
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: What agar sectors should be isolated [Re: snawwz]
#23875756 - 11/28/16 05:14 PM (7 years, 2 months ago) |
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they haven't been isolated. you've done transfers. if you want an isolate 10-20 more or so. I wouldn't bother with an isolate especially at your experience with agar level.
good work
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snawwz
Beginner Cultivator


Registered: 11/07/16
Posts: 139
Last seen: 6 years, 10 months
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Re: What agar sectors should be isolated [Re: bodhisatta]
#23875867 - 11/28/16 05:47 PM (7 years, 2 months ago) |
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Ahh i see. Thanks for the advice. Well, I already have made quite a few Petris, have all the tools, and equipment. So, with practice I feel I'll get better quickest with continuing my agar work. Plus, it's a fun hobby! Ha
So, where should i make the transfer from?
Thanks!
-------------------- snawwz ✌
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: What agar sectors should be isolated [Re: snawwz]
#23875895 - 11/28/16 05:55 PM (7 years, 2 months ago) |
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The fastest leading edges
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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snawwz
Beginner Cultivator


Registered: 11/07/16
Posts: 139
Last seen: 6 years, 10 months
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Re: What agar sectors should be isolated [Re: cronicr]
#23875933 - 11/28/16 06:06 PM (7 years, 2 months ago) |
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So, Is a good rule of thumb to measure the distance from the center to the outside edge and pick the location with the farthest distance?
Does visual content factor into this at all, after several transfers have already been done?
Preciate the help guys!
EDIT: To me, 8-10 o clock on Dish #3 looks the best.
-------------------- snawwz ✌
Edited by snawwz (11/28/16 06:21 PM)
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: What agar sectors should be isolated [Re: snawwz]
#23875990 - 11/28/16 06:20 PM (7 years, 2 months ago) |
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Yeah, grab a rule and get to work
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snawwz
Beginner Cultivator


Registered: 11/07/16
Posts: 139
Last seen: 6 years, 10 months
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Re: What agar sectors should be isolated [Re: Josex]
#23876004 - 11/28/16 06:23 PM (7 years, 2 months ago) |
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Thanks man!
-------------------- snawwz ✌
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: What agar sectors should be isolated [Re: snawwz]
#23876009 - 11/28/16 06:25 PM (7 years, 2 months ago) |
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I was kidding ma dude. You select the leading edge visually.
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snawwz
Beginner Cultivator


Registered: 11/07/16
Posts: 139
Last seen: 6 years, 10 months
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Re: What agar sectors should be isolated [Re: Josex]
#23876031 - 11/28/16 06:32 PM (7 years, 2 months ago) |
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Haha I gotcha. I mean, like, does thickness, color, etc. factor in or should I just go by leading edge?
These convos really help me so thanks again ✊
-------------------- snawwz ✌
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 26 minutes
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Re: What agar sectors should be isolated [Re: snawwz]
#23876210 - 11/28/16 07:31 PM (7 years, 2 months ago) |
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Agar is kind of trickey to see and know. In this pic I am not sure if I selected clean rizo growth or if the myc. went rizo to escape some form of contam.

That is why I love me a fresh fruitbody inner stem flesh clone.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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snawwz
Beginner Cultivator


Registered: 11/07/16
Posts: 139
Last seen: 6 years, 10 months
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Re: What agar sectors should be isolated [Re: wtfcrazymofo]
#23876895 - 11/28/16 11:51 PM (7 years, 2 months ago) |
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Damn I don't have that eye yet lol. I just read some RR posts on isolation tho, and that answered a lot of questions.
I'm curious to see what other members say about your dish.
-------------------- snawwz ✌
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