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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24143897 - 03/07/17 01:36 PM (6 years, 10 months ago) |
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I forgot to mention, you can make enriched fluids from vegetables, such as carrots, for small scales. It is possible to use it as a liquid culture or agar additive, even non organic works.
Recipe:
Optional: Add lime for live mycelium transfers.
100g > Carrots, vegetable waste. 200g > Hot kettle water.
Dice or blend the vegetables, add the hot water, mix-mash for 10 minutes. Strain using a strainer, add kitchen towel to catch the mush.
Assemble and pressure cook, be sterile (clean).
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24151005 - 03/10/17 10:23 AM (6 years, 10 months ago) |
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Here is my wild Summer oyster sample, roughly 5 weeks old, still fresh. Stored in CaCO3 water. It's in a fridge at roughly 2-4°C, I added a sterilized wooden toothpick for transfer.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24151011 - 03/10/17 10:24 AM (6 years, 10 months ago) |
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Once the toothpick is colonized, I can remove it and transfer to agar or other medium.
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Mattisfat
Learning


Registered: 01/20/16
Posts: 570
Loc: Newcastle, England
Last seen: 3 years, 21 days
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24151289 - 03/10/17 12:13 PM (6 years, 10 months ago) |
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hows the cyan coming on?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Mattisfat]
#24151342 - 03/10/17 12:34 PM (6 years, 10 months ago) |
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Still working on the cyans, I have a spore print I also intend to use soon. For me it's a side project, I'm not really focusing on it, atm.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24151377 - 03/10/17 12:45 PM (6 years, 10 months ago) |
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I seems rich compost provides the quick feed that I need (solubles, small units, broken down materials). Here I am using a cheap compost with added peat, I added CaCO3 water to increase the pH.
The change to pH will cleanse the compost and also breakdown acids further.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24151382 - 03/10/17 12:47 PM (6 years, 10 months ago) |
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My next test recipe will be WL-Tek as normal with 25g (dry), hydrated compost added, no tea.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24154002 - 03/11/17 01:18 PM (6 years, 10 months ago) |
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Notable nutrients:
Paper Pellets -- Carbon, Chromium, Copper, Iron, Potassium, Magnesium, Manganese, Phosphorus, Silicon. Compost -- Sulfur, Carbon, Magnesium, Calcium, Boron, Copper, Iron, Iodine.
Missing, insufficient:
Micro nutrients -- Sodium, Zinc, Selenium.
Vitamins -- Vitamin D, Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Pantothenic Acid, Biotin, Ascorbic Acid.
Macro nutrients -- Nitrogen.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24154004 - 03/11/17 01:19 PM (6 years, 10 months ago) |
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Compost also adds small units as I mentioned, allowing the mycelium to populate faster.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24160892 - 03/14/17 07:14 AM (6 years, 10 months ago) |
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Here is my wild Summer oyster tissue, now about 6 weeks old. Slowly growing out at a low 2-4°C in CaCO3 water.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24160923 - 03/14/17 07:35 AM (6 years, 10 months ago) |
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I put my King oyster stunts into CaCO3 water yesterday, they appear to be self cleaning. The browning due to aborting is disappearing, there is some metabolites.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24164217 - 03/15/17 12:34 PM (6 years, 10 months ago) |
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It looks like vegetable extract agar (VEA) is working, I did limited testing with carrot water as a liquid culture. Another member has messaged me that they had 12 hour success with a homemade VEA recipe.
Said member is still tinkering, but I asked them to post a recipe when ready.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24164231 - 03/15/17 12:40 PM (6 years, 10 months ago) |
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Here is WL-Tek paper to sugar rich raw carrot (cooked).
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24164449 - 03/15/17 02:32 PM (6 years, 10 months ago) |
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Here I'm trying some cyan spores to mildly enriched tea bags. Tea bags are easy to clean, I'm using a microwave.
I took 800g of hot kettle water, I added 20ml of enrichment tek and 1.5 teaspoons of sucrose. The two tea bags where added, I stewed with a fork then left it to cool, fully covered.
Once cooled I microwaved for 8 minutes, strained and assembled raging hot.
I'm just waiting for it to cool down before adding spores.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24164453 - 03/15/17 02:35 PM (6 years, 10 months ago) |
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This is also my first homemade spore syringe from a print. I am using a SAB for assembly.
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24164844 - 03/15/17 05:31 PM (6 years, 10 months ago) |
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Quote:
Ferather said: Here is WL-Tek paper to sugar rich raw carrot (cooked).

Very cool
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: TravelAgency]
#24166963 - 03/16/17 12:46 PM (6 years, 10 months ago) |
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Any vegetable water will work.
Carrot (or peel) looks like a honey or karo LC. Pea or green veg water is of course green. Potato (or peel) is more white-clear.
You can also cook wooden toothpicks in your chosen water for testing on agar. I would add further relevant colour to the peg for identification.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24167315 - 03/16/17 03:02 PM (6 years, 10 months ago) |
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Data share:
Ergosterol -- [C]28--[H]44--O Glycogen -- [C]6--[H]10--[O]5
Notes:
Cellulose -- [C]6--[H]10--[O]5 x n, (same as glycogen) Starch -- [C]6--[H]10--[O]5 x n, (same as glycogen) Sugar -- [C]n--[H]2n--[O]n, (varies with the sugar)
n = Number of total units linked.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24167321 - 03/16/17 03:03 PM (6 years, 10 months ago) |
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This explains why adding carbon [C] to water [H2O] produces basic blocks.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24170200 - 03/17/17 03:10 PM (6 years, 10 months ago) |
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Pocket guide updated, enjoy. Link is in my signature.
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