|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23903253 - 12/07/16 12:52 PM (7 years, 1 month ago) |
|
|
I added the substrate at 28°C, so there is a little condensate. Growth should begin after 24 - 48 hours, roughly.

Assembly pH is 6.8 - 7.0 approx.
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23905944 - 12/08/16 07:21 AM (7 years, 1 month ago) |
|
|
The plant fiber spawn, is pretty amazing. Instant response, little delay. Very strong, dense, aggressive, fast and healthy growth.

Much faster and stronger than grain.
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23905948 - 12/08/16 07:25 AM (7 years, 1 month ago) |
|
|
Side note pH made a huge difference to BE and speed.
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23909343 - 12/09/16 07:32 AM (7 years, 1 month ago) |
|
|
Here is 48 hours later for my TGF-Tek spawn.

Shouldn't take very long.
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23910470 - 12/09/16 02:39 PM (7 years, 1 month ago) |
|
|
Soon I will be trying a special T-Gel, pH 6 via lime, with a very special additive. The additive should improve growth, and keep the T-Gel starch free.
Here is day 3 for the Tarragon TGF-Tek spawn:
Edited by Ferather (12/10/16 03:02 PM)
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23918859 - 12/12/16 08:28 AM (7 years, 1 month ago) |
|
|
Tarragon oyster on TGF-Tek, pH 7, day 5:
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23919582 - 12/12/16 01:17 PM (7 years, 1 month ago) |
|
|
Optimized germination conditions, notes so far:
Temperature: 20 - 28°C | Water content: 80 - 90% | pH level: 4.5 - 7.5 (lower preferred) | CO2 level: 25%
Prevention in a casing: pH 8 @ 66 - 75% water content, lower the temperature, increase O2. Prevention in a substrate: pH 7 @ 66 - 75% water content, lower the temperature.
|
Pinpapa
Stranger than most


Registered: 09/01/12
Posts: 259
Loc: Algonquin park
Last seen: 3 years, 10 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23919971 - 12/12/16 03:04 PM (7 years, 1 month ago) |
|
|
Ferather If I am ballparkin ph with lime @how many mg percup withh one bag.I love this tea bit!
-------------------- "This isn't the right thing to do , so let's go"
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Pinpapa]
#23920055 - 12/12/16 03:28 PM (7 years, 1 month ago) |
|
|
Brewed tea is roughly pH 5.6, 3 - 4g to 125g water. It variates in steeping time and water. About 2 - 4g of lime to the end result should be close without a pH reader.
|
Pinpapa
Stranger than most


Registered: 09/01/12
Posts: 259
Loc: Algonquin park
Last seen: 3 years, 10 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23920068 - 12/12/16 03:31 PM (7 years, 1 month ago) |
|
|
I have a meter but its old & I dont trust it besides it has to be adj for temp I think. Medicinals seem to be on a popularity spike.The woman at the book store said everyone is asking bout mushrooms.I live on 100 acres of swampy bush I plan to benefit and promote the little buggers.As well as share methods and supplies.Also want to produce woodlover kits.I gotta ease off on the Reishi my mind is spinning @60000rpm.But I feel great! That terr/Oysta sure is pretty for five dayz
Edited by Pinpapa (12/12/16 03:41 PM)
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Pinpapa]
#23920094 - 12/12/16 03:39 PM (7 years, 1 month ago) |
|
|
You can get a digital pH reader from Amazon or eBay for cheap. White litmus paper is easy to get and very cheap.
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23928903 - 12/15/16 10:50 AM (7 years, 1 month ago) |
|
|
The TGF-Tek spawn is working as intended, open air assembly. No mold, no bacteria and no yeast. I also skipped the pressure cooker step, so microwave PC only for 24 minutes.
The tek has been updated to 40g of grain flour, up from 20g.
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23928980 - 12/15/16 11:15 AM (7 years, 1 month ago) |
|
|
Needs more quick energy (20g), but not nutrients.

The yellow tinted stripe is my torch.
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23939867 - 12/19/16 08:29 AM (7 years, 1 month ago) |
|
|
Here is a Summer oyster sample into TGF-Tek, 10mm under the surface. This was to increase strength and multi-directional growth.
Open air assembly, microwave only. 48 Hours:
|
Mattisfat
Learning


Registered: 01/20/16
Posts: 570
Loc: Newcastle, England
Last seen: 3 years, 21 days
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23940129 - 12/19/16 11:28 AM (7 years, 1 month ago) |
|
|
Quote:
Ferather said: Needs more quick energy (20g), but not nutrients.

The yellow tinted stripe is my torch.
That myc looks incredible.
Hows your contam rate going atm with your experimental subs?
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Mattisfat]
#23940169 - 12/19/16 11:49 AM (7 years, 1 month ago) |
|
|
I haven't made enough of my new TGF-Tek to give an accurate figure. 2/2 clean so far. Here is that Tarragon, now in phase 2 consolidation and surface growth.
At a guess, I would say another 3 - 4 days left.
|
Pinpapa
Stranger than most


Registered: 09/01/12
Posts: 259
Loc: Algonquin park
Last seen: 3 years, 10 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23942638 - 12/20/16 08:05 AM (7 years, 1 month ago) |
|
|
I got the idea to use spent green tea for spawn from one of your posts.I've been workin azure spores with little success until I introduced the tea. It ripped right through the tea and transferred to alder chips in less than 48 hrs.I have since then ,started an old fricosa (when purchased) with amazing speed.And I drink the tea!I live in a bacterial prone house, this has helped immensely.Thanks for sharing.
-------------------- "This isn't the right thing to do , so let's go"
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: Pinpapa]
#23942654 - 12/20/16 08:13 AM (7 years, 1 month ago) |
|
|
Your very welcome. Thank you for testing azure's. It should also rip through TGF-Tek, being a wood lover.
|
leschampignons
Biochemistry + Mycology


Registered: 08/30/13
Posts: 1,583
Loc: NY/NJ/ME
Last seen: 4 days, 8 hours
|
Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23942718 - 12/20/16 08:44 AM (7 years, 1 month ago) |
|
|
Don't know if you are still trying to isolate bacteria, but I agree with the differential media suggestion that drake mentioned. Another thing that might help is quick, high heat treatment like maybe 80 degrees C for 30 mins. combined with differential media you can isolate heat tolerant bacteria like Bacillus.
|
Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
|
Re: Ferather's Corner | Experiments, tests and data [Re: leschampignons]
#23942753 - 12/20/16 08:59 AM (7 years, 1 month ago) |
|
|
Currently I am bumped at the expense of the materials needed to make differential media. Still trying to figure out if there is another way, thanks for the heat idea.
|
|