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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24199918 - 03/28/17 02:07 PM (6 years, 9 months ago) |
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You can also add materials to the bath, such as lime or anything soluble. Add other materials to germinate, and make a capture cup.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24200294 - 03/28/17 04:34 PM (6 years, 9 months ago) |
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I replaced and added new pegs to my Tarragon oyster. Using a simple pair of cleaned metal tweezers.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24200295 - 03/28/17 04:34 PM (6 years, 9 months ago) |
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Marty your two pegs are being sent tomorrow, they are growing already.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24205546 - 03/30/17 02:22 PM (6 years, 9 months ago) |
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The Tarragon sample to capture cup is working, it started growing out after roughly 28 hours. It has pushed through the bottom of the inner cup, and started growing up the sides.
It will probably take 6-8 days to reach the wooden transfer pegs.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24205551 - 03/30/17 02:23 PM (6 years, 9 months ago) |
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The capture cup was not enriched in any way, 100% plain (other than nutrients in water).
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24207748 - 03/31/17 10:11 AM (6 years, 9 months ago) |
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As many capture cups as eggs in the carton, plus a paper tray.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24208028 - 03/31/17 12:12 PM (6 years, 9 months ago) |
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Here is my Tarragon paper sample to capture cup.

Edited by Ferather (03/31/17 12:27 PM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24210708 - 04/01/17 12:01 PM (6 years, 9 months ago) |
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I scraped some side growing Tarragon mycelium from my WL-Tek paper spawn. The sample was placed in the latest WL-Tek recipe, no tea or calcium.
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Ferather
Mycological



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Posts: 6,325
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24210716 - 04/01/17 12:05 PM (6 years, 9 months ago) |
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I intend to expand the Tarragon mycelium into its fruiting substrate 4 times in total. Starting small and expanding to full size, this allows for full adaption.
The following 3 expansions will have added CaCO3.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24214888 - 04/03/17 09:45 AM (6 years, 9 months ago) |
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Well the compost and peat mix is composed of complex energy, making them open air safe. The scraping has no simple energy to digest-reassemble, so it grows very slowly.
No quick energy, no speed, even when enriched and at pH 6, no lime. I can add a few drops of pH 8.4, CaCO3 + sugar water.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24215433 - 04/03/17 02:01 PM (6 years, 9 months ago) |
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Utilizing paper pellets
As an alternative to vermiculite:
100g-125g of paper pellets (as body | cellulose + basic nutrients | up to 80-85% water content). Add: 50g wood powder, 50g compost powder, and additional macro-micro nutrients.
Adjust the pH to as high as possible using CaCO3 water (pH 8-10).
As a colonizable barrier:
Hydrate fully to 80% using boiling hot water, allow to cool fully. Optional: Hydrate with CaCO3 water to increase the pH.
Also usable to produce clean transfers.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24217290 - 04/04/17 08:55 AM (6 years, 9 months ago) |
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Some basic maths, ignoring any present water content (usually about 5%). 100g paper pellets @ 80% water (x4 as water, 400g). 50g wood powder @ 66% water (x2 as water, 100g). 50g compost powder @ 66% (100g). Total water to add: 600g. | 200g dry, x3 = 600g, so you have 75% overall.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24217292 - 04/04/17 08:56 AM (6 years, 9 months ago) |
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If you intend to add for example 50g of whole grain flour, due to the binding nature of starch you will need to assemble and cook for 8 minutes. Allow to fully cool, about 2-4 hours, at this point the paper will have fully absorbed the grain flour and will return to a crumble.
Continue with assembling into bags-other for full sterilization using your normal methods.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24220837 - 04/05/17 03:32 PM (6 years, 9 months ago) |
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Here is the basic WL-Tek medium, and the new pegs. Lots of side growth to transfer.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24220838 - 04/05/17 03:32 PM (6 years, 9 months ago) |
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The Tarragon transfer colony is currently growing at 12°C in a fridge.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24223060 - 04/06/17 02:36 PM (6 years, 9 months ago) |
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Here is my Psilocybe Cyanescens from spores on sucrose + enrichment tek. They germinated at 12°C after 9 days, now at room temperature.
I have it a good swirling, no bubbles or cloudy slime.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24223061 - 04/06/17 02:36 PM (6 years, 9 months ago) |
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Recipe: 2g sucrose , 1-2g enrichment tek, 20g boiling hot water.
You may need to adjust the pH to suit your needs.
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24223095 - 04/06/17 02:54 PM (6 years, 9 months ago) |
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Quote:
Ferather said: Here is my Psilocybe Cyanescens from spores on sucrose + enrichment tek. They germinated at 12°C after 9 days, now at room temperature.
I have it a good swirling, no bubbles or cloudy slime.

Beautiful!
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: TravelAgency]
#24223111 - 04/06/17 03:01 PM (6 years, 9 months ago) |
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Job done, I will be inoculating WL-Tek paper, basic, no tea, compost or lime. Which will be used as a transfer colony to make clean spawn.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#24225266 - 04/07/17 10:49 AM (6 years, 9 months ago) |
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Here is the plain Tarragon capture cup, growth is pathetic without added macro-micro nutrients. I can however transfer the pegs to a more rich medium to produce better growth.
Fertilizers do NOT prevent growth after transfer to a weak medium.
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