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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Ferather's Corner | Experiments, tests and data
#23875139 - 11/28/16 12:48 PM (7 years, 2 months ago) |
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Welcome to my thread of experiments, tests, data, web-links and other useful information. Feel free to discuss, suggest or correct anything along side any posts.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23875170 - 11/28/16 01:03 PM (7 years, 2 months ago) |
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I'm currently experimenting with infected spawn and an attempt to cultivate bacteria and yeast from it. The current application is a basic LC enriched with nutrients that boost bacteria and yeast.
So far in 1 hour I have active bacteria and yeast, however mycelial activity too. I have a composter full of tons of bacteria that I can use instead.
Is there a quick and safe way to obtain active bacteria?
Here is the LC after 2 minutes, bubbles.

1 hour later, all 3 seem active.

Ok with mycelium too?
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adadada
Stranger


Registered: 01/18/16
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23875259 - 11/28/16 01:54 PM (7 years, 2 months ago) |
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uhhh ... why?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: adadada]
#23875279 - 11/28/16 02:04 PM (7 years, 2 months ago) |
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I need the bacteria to test the antibacterial effects of tea, infused into materials such as grain. In order to get accurate results I must first cultivate a bacteria to inhibit on grain.
So any suggestions are greatly appreciated, I have syringes etc. I have assembled a make shift SAB for the cultures.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23875599 - 11/28/16 04:06 PM (7 years, 2 months ago) |
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This is probably nothing, but something looks like a black poplar pin that is trying to grow out.

It could be some shell from another seed, I don't know.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23875663 - 11/28/16 04:27 PM (7 years, 2 months ago) |
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Interesting with that pin looking thing on the grain.
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Re: Ferather's Corner | Experiments, tests and data [Re: Marty Mycfly]
#23875784 - 11/28/16 05:25 PM (7 years, 2 months ago) |
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Do they all have tea and what is going to be your control?
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Quadman
Challenged


Registered: 04/23/16
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Re: Ferather's Corner | Experiments, tests and data [Re: vatman]
#23875860 - 11/28/16 05:46 PM (7 years, 2 months ago) |
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There are so many bacteria possibilities that your variables are going to be huge for experimenting. Not to mention fungal. I admire the effort.
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Re: Ferather's Corner | Experiments, tests and data [Re: Quadman]
#23876450 - 11/28/16 08:39 PM (7 years, 2 months ago) |
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Well if he just isolates some bacteria or could use mouth swabs and compare growth rate.
He will get bacteria growth sooner later. If bacteria is considerably slower than the control than we know the tea has some affect on the bacteria in a controlled aspect.
Yes I see your point. The best option might just be isolating contamination and see how it works on normal agar vs tea
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: vatman]
#23877333 - 11/29/16 06:07 AM (7 years, 2 months ago) |
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None have tea. All I need out of this is bacteria and yeast. LC is just easier for syringe and then inject. I am using dirty bacteria and yeast infected cheap vendor spawn, easy to handle and active.
Once I have a bacteria and yeast LC I will infect tea infused materials.
The LC is now a green beer.

And dirty WBS spawn.

I can also take a WBS sample and soak it in limed tea water for 4 days. Then see if the sample colonizes with no active bacteria.
Any suggestions or improvements?
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drake89
Mushroom Magnate



Registered: 06/26/11
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23877351 - 11/29/16 06:21 AM (7 years, 2 months ago) |
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: drake89]
#23877371 - 11/29/16 06:43 AM (7 years, 2 months ago) |
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Thanks Drake, I found this after reading your post. I can match most of those nutrients now. But Ph 7.2 interesting, my LC is Ph 7 @ 22°C.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23877419 - 11/29/16 07:27 AM (7 years, 2 months ago) |
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Here is another agar list I have found. But it is still very expensive to buy.
Edit: removed, too expensive.
Edited by Ferather (11/29/16 07:55 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23878125 - 11/29/16 12:19 PM (7 years, 2 months ago) |
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So reading those recipe's it seems my mixture will birth and grow pretty much anything. Unfortunately indicators needed to prove presence are very expensive to buy.
Colour of the LC changed from cloudy-blue to green-yellow.
Here is assembly and also 24 hours later.

Good enough for intended use?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23878384 - 11/29/16 01:58 PM (7 years, 2 months ago) |
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I put some of that dirty WBS spawn to limed tea water, fully soaking. Bubble production is slowly stopping. There are a few things I can do after the 4 days, use the water, and the spawn for testing.
Bacteria present should stall, and hopefully I will have something usable.
There should be enough to attempt self re-colonization.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23880893 - 11/30/16 08:06 AM (7 years, 1 month ago) |
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So after 48 hours I have rife bacteria and yeast.

Now being stored for testing at 2°C.

Ready to infect test samples.
Here is the dirty WBS spawn soak assembly.

Almost no bubble production.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23882095 - 11/30/16 03:35 PM (7 years, 1 month ago) |
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Well katbusa beat me to testing, however I will continue with testing tea infused spawn mixtures. The dirty WBS spawn soak is going well, no changes so far, no bubbles or scum.
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katbusa
TC Enthusiast


Registered: 02/19/13
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23882104 - 11/30/16 03:38 PM (7 years, 1 month ago) |
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No! LOL by all means do the test as well.. Having two people to verify the same thing is essential. It adds more credibility to using tea.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: katbusa]
#23882132 - 11/30/16 03:45 PM (7 years, 1 month ago) |
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Cooking some rye either tomorrow or Friday. Going to SAB and syringe one with tea one without. Bit of a random stab and tea ratio and saturation level, either way, some results.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
#23883693 - 12/01/16 05:36 AM (7 years, 1 month ago) |
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Here is the dirty WBS soak after nearly 48 hours.

No bubbles, no scum, no slime.
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