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Offlinewild child
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Registered: 08/06/11
Posts: 122
Last seen: 5 years, 7 months
I am giving up
    #23871273 - 11/27/16 07:52 AM (7 years, 2 months ago)

I am not sure what else to do...every jar (brf) ive done in the past several months all become Bacillus..Ive had many successful grows, with less sterile technique..so I am very confused here.

each syringe ive used comes from reputable places. I steam for 1h 30min to 2 hrs. use a SAB, gloves, flame sterilize between each jar. use less water each time...nothing works. ive heard vermiculite doesnt go bad, that would be the only thing left for me to try is to buy new verm. I dont think even a PC would stop this issue.

I think this is my calling, time to retire...its been fun guys.


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OfflineYrat
Hello

Registered: 11/08/07
Posts: 2,312
Last seen: 2 years, 10 months
Re: I am giving up [Re: wild child]
    #23871280 - 11/27/16 07:56 AM (7 years, 2 months ago)

a pc WOULD stop the issue if it is indeed contaminated verm... steaming does not sterilize, but pressure cooking does.


--------------------
"There are a thousand hacking at the branches of evil
to one who is striking at the root."
-Henry David Thoreau
Strike The Root


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Offlineunfortunategent
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Registered: 06/04/14
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Last seen: 2 years, 2 months
Re: I am giving up [Re: Yrat]
    #23871318 - 11/27/16 08:25 AM (7 years, 2 months ago)

I think if bacteria is the only issue, you're pretty easily defeated.  I could see if it was mold...

Have you bought new syringes?  Have you tried agar?  And small PC's are pretty cheap if you only need one big enough for cakes.


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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,342
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Re: I am giving up [Re: Yrat]
    #23871323 - 11/27/16 08:28 AM (7 years, 2 months ago)

Quote:

Yrat said:
steaming does not sterilize




:facepalm:

yes, it does


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InvisibleMad Season
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Registered: 09/16/12
Posts: 12,666
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Re: I am giving up [Re: wild child]
    #23871359 - 11/27/16 08:47 AM (7 years, 2 months ago)

Quote:

wild child said:
I am not sure what else to do...every jar (brf) ive done in the past several months all become Bacillus..Ive had many successful grows, with less sterile technique..so I am very confused here.

each syringe ive used comes from reputable places. I steam for 1h 30min to 2 hrs. use a SAB, gloves, flame sterilize between each jar. use less water each time...nothing works. ive heard vermiculite doesnt go bad, that would be the only thing left for me to try is to buy new verm. I dont think even a PC would stop this issue.

I think this is my calling, time to retire...its been fun guys.



I think this is your calling to test your syringes on agar, and transfer until clean. No matter how reputable the syringe source is, if there's a lot of bacillus in there, you're screwed.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineTerpfreak
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Registered: 07/08/16
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Loc: Land Of Ooo
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Re: I am giving up [Re: Mad Season]
    #23871364 - 11/27/16 08:49 AM (7 years, 2 months ago)

:cuteshit:


--------------------


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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
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Re: I am giving up [Re: Terpfreak]
    #23871372 - 11/27/16 08:52 AM (7 years, 2 months ago)

I used to inoculate in the open air, even do g2g transfers with reasonable success in the open air. I moved and now I have had SO much trouble. I mean it was hard to even get a tub to flush before getting overtaken by green mold. I wouldn't even attempt a g2g transfer, fuck. I built a flow hood, did agar work, and now have much higher success rates. I don't know if it's because of the air or my source syringes were just dirty, I wouldn't trust any vendor to give me a sterile syringe so it could be either or.


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Offlinewild child
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Registered: 08/06/11
Posts: 122
Last seen: 5 years, 7 months
Re: I am giving up [Re: unfortunategent]
    #23871380 - 11/27/16 08:58 AM (7 years, 2 months ago)

Quote:

unfortunategent said:
I think if bacteria is the only issue, you're pretty easily defeated.  I could see if it was mold...

Have you bought new syringes?  Have you tried agar?  And small PC's are pretty cheap if you only need one big enough for cakes.





have bought several new syringes, I dont know how to use Agar, I need a PC for that correct? I am thinking its my Verm.


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OfflineTerpfreak
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Re: I am giving up [Re: wild child]
    #23871383 - 11/27/16 08:59 AM (7 years, 2 months ago)

Use agar, then give up. Never before.

You have no way of knowing what it is without using agar, but WITH pasty plates or petri dishes you can easily figure out what the issue is down to the hand movements you made that flung bacteria into your culture. 



Look up PastyWhite's PastyPlate tek. Munchauzen's video's and Azur's monotub tek and info.


--------------------


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Offlinewild child
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Registered: 08/06/11
Posts: 122
Last seen: 5 years, 7 months
Re: I am giving up [Re: Terpfreak]
    #23871394 - 11/27/16 09:04 AM (7 years, 2 months ago)

Quote:

Mad Season said:
Quote:

wild child said:
I am not sure what else to do...every jar (brf) ive done in the past several months all become Bacillus..Ive had many successful grows, with less sterile technique..so I am very confused here.

each syringe ive used comes from reputable places. I steam for 1h 30min to 2 hrs. use a SAB, gloves, flame sterilize between each jar. use less water each time...nothing works. ive heard vermiculite doesnt go bad, that would be the only thing left for me to try is to buy new verm. I dont think even a PC would stop this issue.

I think this is my calling, time to retire...its been fun guys.



I think this is your calling to test your syringes on agar, and transfer until clean. No matter how reputable the syringe source is, if there's a lot of bacillus in there, you're screwed.



Quote:

Terpfreak said:
Use agar, then give up. Never before.

You have no way of knowing what it is without using agar, but WITH pasty plates or petri dishes you can easily figure out what the issue is down to the hand movements you made that flung bacteria into your culture. 



Look up PastyWhite's PastyPlate tek. Munchauzen's video's and Azur's monotub tek and info.






cool, I need to do some homework on Agar, thanks


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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: I am giving up [Re: wild child]
    #23871400 - 11/27/16 09:06 AM (7 years, 2 months ago)

It's not your vermiculite, that doesn't make sense. Your vermiculite is loaded with spores, that's why we sterilize it. If it's your vermiculite it means it's because you're not properly sterilizing it, not that there's something inherent to the verm itself. Before getting into agar I'd get a pressure cooker, I wouldn't even attempt to grow mushrooms w/o one.


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Offlinewild child
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Registered: 08/06/11
Posts: 122
Last seen: 5 years, 7 months
Re: I am giving up [Re: krypto2000]
    #23871415 - 11/27/16 09:15 AM (7 years, 2 months ago)

yeah, im gonna have to get a PC. my pot lids have one of those vent holes, so some steam is getting out, I dont know if that has anything to do with it but I even steam for up to 2 hours so I feel that's good enough.


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
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Re: I am giving up [Re: wild child]
    #23871424 - 11/27/16 09:20 AM (7 years, 2 months ago)

Well definitely get a pc if you can. If you can't, agar is a liquid media. It will sterilize much quicker than solid medias (pf tek) due to epic convection. Agar as well doesn't need a PC.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineTh3Issu3
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Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
Re: I am giving up [Re: Mad Season]
    #23871569 - 11/27/16 10:23 AM (7 years, 2 months ago)

When I first got into this I went through a period where everything was infected... Come to find out it was from a lot of dirty syringes. I was pressure cooking, doing everything right. Then It dawned on me, holy shit its the syringes.

I think that's what you're going through.

Get some potato dextros agar. It's a little more but its premixed and one container will last you a long time. Instruction are on the bottle on how to make it. Just add water :smile:

If i where you, Id get some new Syringes too. Oh,and a PC from walmart is only like 60$ i believe.

Edit: dont give up, there are a ton of us on here to help you along the way as long as you do your research.


Edited by Th3Issu3 (11/27/16 10:24 AM)


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OfflineAK1000
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Registered: 06/06/16
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Re: I am giving up [Re: Th3Issu3]
    #23871587 - 11/27/16 10:30 AM (7 years, 2 months ago)

I worked for a couple months with grain that *looked* fine but was not clean. now I do everything with agar. 

Go get some mini rounds from the store and some agar from some asian store and a scalpel from an art store and you could follow pastywhytes agar tek: https://www.shroomery.org/forums/showflat.php/Number/19208976


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


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InvisibleRaven44
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Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
Re: I am giving up [Re: AK1000]
    #23871652 - 11/27/16 10:53 AM (7 years, 2 months ago)

U can't give up before u have even began.

If u haven't ever used a PC for ur grows u haven't even begun to grow.

Hey OP, my advice... get a pressure cooker and start growing using rye grains.

I've heard minimal about steam baths. What I have heard tho I was told they inconsistent.

I think most people start out with a pressure cooker tho.

Props to u for ur dedication. Now show ur dedication some more and get a PC. It will fix your problems. A pc and still air box


Edited by Raven44 (11/27/16 11:05 AM)


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OfflinePeteyboy
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Registered: 06/21/16
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Re: I am giving up [Re: Raven44]
    #23871674 - 11/27/16 11:00 AM (7 years, 2 months ago)

DONT QUIT DUDE!!!! Nothing good in life is easy!!! You gotta get yourself a PC brother, then prep up some agar. None of us knew how to do agar before we said "Fuck it lets try this shit out"!

You can do it man, you have the best people in the world at your fingertips for any advice or help you need. I would be more then happy to help anyway i can!!:jah:


--------------------


*TRADE LIST* https://www.shroomery.org/forums/showflat.php/Number/23855008 *TRADE LIST*




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InvisibleMad Season
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Re: I am giving up [Re: Mad Season]
    #23871676 - 11/27/16 11:00 AM (7 years, 2 months ago)

Quote:

Raven44 said:
If u haven't ever used a PC for ur grows u haven't even begun to grow. He obviously can't do anything with Agar to my knowledge with out a pc. Who knows maybe some wise guy made up a way with h2o2 but still... no pc = no Agar I think?



Quote:

Mad Season said:
Well definitely get a pc if you can. If you can't, agar is a liquid media. It will sterilize much quicker than solid medias (pf tek) due to epic convection. Agar as well doesn't need a PC.



I do agree that he should get a PC. Especially for going to grains.

Heat through convection transfer is why you can just Pc agar for 15-30 minutes to sterilize compared to the 2 hours needed for grains. You can quite easily boil agar for 45-1 hr for a sufficient sterilization. There's even a tek on here on steaming pasty plates


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinewtfcrazymofo
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Registered: 07/26/15
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Re: I am giving up [Re: wild child] * 1
    #23871678 - 11/27/16 11:01 AM (7 years, 2 months ago)

Time to hang up the pf tek and clean up those syringes on agar.  It might be hard, and you might learn the hard way more than half the time, But if your not into it and the subject of this thread is "I give up" well...All I can do is send you some love.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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InvisibleRaven44
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Registered: 12/07/13
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Re: I am giving up [Re: wtfcrazymofo]
    #23871688 - 11/27/16 11:05 AM (7 years, 2 months ago)

My bad, didn't know people.practiced steaming pasty plates

I started out with a pressure cooker there so cheap! Why not?

Listen to what mad season said, but still get a PC dude step ur game up

I edited my misinformation


Edited by Raven44 (11/27/16 11:06 AM)


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