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Offlineacidninja
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Spore Print Inoculation
    #23869659 - 11/26/16 04:59 PM (7 years, 3 months ago)

So I was wondering when I get my first GT fruits I was gunna save one and make a spore print, then I was gunna use the spore print in a monotub, but I was always confused as to how you actually inoculate with a spore print? Do you just use a sterilized scalpel and scrape the spores onto the bulk sub or something . . ?

Also, I check my GT jar this morning and the myc has colonized the dry verm in the top of the jar, should I be worried?

Thanks, aj


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OfflinePsilosopherr
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Re: Spore Print Inoculation [Re: acidninja]
    #23869690 - 11/26/16 05:08 PM (7 years, 3 months ago)

People don't typically inoculate bulk subs with spores, doubt it would work.

Do a search on how to make spore syringes. In a nutshell you scrape some spores into a PC'd shotglass, squirt in some presterilized water from a clean syringe and then aspirate. Once you've got spores and water in the syringe just give it a good shake.

You can also use a print directly by scraping onto agar or using an inoculation loop but it doesn't sound like you do agar yet.

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OfflinePsilosopherr
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Re: Spore Print Inoculation [Re: Psilosopherr]
    #23869692 - 11/26/16 05:09 PM (7 years, 3 months ago)

On the dry verm note, I doubt mycelium could colonize it if it were truly dry, perhaps you accidentally squirted solution into the dry verm layer :shrug: dunno

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Offlineacidninja
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Re: Spore Print Inoculation [Re: Psilosopherr]
    #23869826 - 11/26/16 05:42 PM (7 years, 3 months ago)

Alright, thanks man!


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InvisibleThadeous
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Re: Spore Print Inoculation [Re: acidninja]
    #23869955 - 11/26/16 06:14 PM (7 years, 3 months ago)

If you substrate was excessively wet and/or you live in a humid environment I could see myc potentially growing onto the verm.

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Offlineacidninja
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Re: Spore Print Inoculation [Re: Thadeous]
    #23871129 - 11/27/16 05:12 AM (7 years, 3 months ago)

Quote:

Thadeous said:
If you substrate was excessively wet and/or you live in a humid environment I could see myc potentially growing onto the verm.




:shrug:


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