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OfflineBigbadwooof
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Josex]
    #23852695 - 11/20/16 08:51 PM (8 years, 1 month ago)

Quote:

Josex said:
Quote:

Bigbadwooof said:
Quote:

Inocuole said:
If so, can you put your dick in it?




... I don't know, but I probably need to.




Yeah, women like gamma-sterilized dicks nowadays, not the smelly things most of you got.




How about gamma sterilized Penis Envy?


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

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Offlinewtfcrazymofo
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Registered: 07/26/15
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Josex]
    #23854429 - 11/21/16 02:13 PM (8 years, 1 month ago)

Quote:

Josex said:
I keep reading everywhere that trump has a government grant to grow mashrooms. Can someone link to the OP where he said that? Fucking missed it!! :wtfsonic:



utsf


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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InvisibleJosex
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: wtfcrazymofo]
    #23854439 - 11/21/16 02:19 PM (8 years, 1 month ago)

I did already mang, I was just hoping someone would spare me the gruelling effort.

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Offlinewtfcrazymofo
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Josex]
    #23854482 - 11/21/16 02:35 PM (8 years, 1 month ago)

That is a common word posted by tump. 

OP I wish I could whip up some clean old plastic plates, but If I wanted to save them I'd just buy the expensive glass ones. 

It is like trying to reuse a plastic diaper or a cloth diaper.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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Offlinekrypto2000
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: wtfcrazymofo]
    #23854493 - 11/21/16 02:41 PM (8 years, 1 month ago)

Plastic diapers are reusable, just stick it under a UV lamp for a few hours.

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OfflineBigbadwooof
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: krypto2000]
    #23854630 - 11/21/16 03:27 PM (8 years, 1 month ago)

Quote:

krypto2000 said:
Plastic diapers are reusable, just stick it under a UV lamp for a few hours.




Nah, too much light. Put it next to your heat register for a month or so :wink:


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

Edited by Bigbadwooof (11/21/16 03:27 PM)

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OfflineBigbadwooof
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
    #23854634 - 11/21/16 03:28 PM (8 years, 1 month ago)

Quote:

r.lutece said:
From Experiments Using Non-Sterile Plates:

Quote:

Reusing Plastic Petri Plates.
The standard dish is 100 x 15 mm. Most of them are used once and discarded. I save mine and try to figure ways to reuse them. Also in my plant micropropagation lab I use a special dish which is 100 mm dia and 50 mm tall and very expensive. Plastic dishes are made of polystyrene because it is crystal clear, but it crumples when autoclaved. The factory uses Cobalt-60 gamma radiation to sterilize them after they are packed in tough sealed platic bags.
I sterilize them in 10% Chlorox (undiluted it is 5.25 % sodium hypochlorite). Make this by adding one volume of Chlorox to 9 parts tap water. This 10% Chlorox is used in homes, farms, and labs to sterilize many things, but it will corrode iron and many other metals.
If leave the bleach on the plates, your experiments may be killed unless there is enough dilution. Therefore I dip them in sterile autoclaved water or rubbing alcohol which evaporates faster. However for most uses the wet Petri plates are fine.
In my micropropagation lab, I fill the wet plates with sterile agar which was autoclaved in bottles or jars and plant my strawberry plants. Growth takes weeks, but very few dishes become contaminated. How long must you soak them to kill all the bacteria? Killing is a logarithmic function. You will never kill every organism in a million plates. I suggest you do a test. I let them soak 20 miuntes and I have no problems. Therefore less soaking would sterilize most plates. I handle them with sterile forceps. When not using the forceps, I stand them in 70% iso-propanol--bleach will corrode metals. You could use 70% iso-propanol to sterilze your plates, but bleach is cheaper. Of course I wash the plastic items before sterilizing them with 10% Chlorox.







I say this is the best answer ^^^

I will be trying this. Although, I wouldn't do it without a hood.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

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Offlinetump
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
    #23859710 - 11/23/16 07:17 AM (8 years, 1 month ago)

I think i found the answer. Just cleaning them out, pouring grain water agar into them and dry heat sterilize then at 170 for 2.5 hours while your grain pc. I did this five days ago with 8 of them and they haven't molded out yet. I stream some 6 days ago and all molded clusters this morning

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Invisibler.lutece
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
    #23859962 - 11/23/16 09:04 AM (8 years, 1 month ago)

Did you use the oven for dry sterilization?


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.

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Offlinetump
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
    #23860231 - 11/23/16 10:32 AM (8 years, 1 month ago)

Yes i did. Streaming cracks the lids. Im waiting a 30 days without contams before i can say reuseing with dry heat works.

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OfflineBigbadwooof
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
    #23860438 - 11/23/16 12:14 PM (8 years, 1 month ago)

Quote:

tump said:
Yes i did. Streaming cracks the lids. Im waiting a 30 days without contams before i can say reuseing with dry heat works.




I would imagine that dry heat may work for mold, but it wouldn't likely solve any bacterial problems. You may still get away with it regardless.

The other day I was pouring some plates in front of my hood, and I accidently nudged a stack of them just outside the flow. They hadn't been wrapped in parafilm yet, btw... All of them seem perfectly fine! I was a bit surprised.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

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Offlineblackout
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
    #23860554 - 11/23/16 12:58 PM (8 years, 1 month ago)

Quote:

r.lutece said:
Quick question: doesn't a vinegar/bleach solution put off chlorine gas?


Yeah, it does, so you have to take precautions. Bleach is usually sold with sodium hydroxide, aka lye, aka caustic soda mixed in. This stabilizes it, the vinegar neutralizes it and makes it much more effective and also more unstable, which is what I actually want. It is commonly used by homebrew people to make a "no rinse sanitiser", i.e. they wash bottles out with it, leave them upside down to drain and do not bother rinsing anymore, they pour beer right into them. I would use a stronger concentration than them.

I tried it before and dried them out on a heat pad, however I was very haphazard doing it all so most did fail but I still think it is worth trying again. Some are still OK many months on, I just left them be.


Quote:

Bigbadwooof said:
rinse them in a bath of sterilized water in front of your flowhood (if you have a hood).


Rather than an actual bath I was thinking of a bottle of sterile water, as an actual bath would be hard to work with as it would be easily contaminated with dipping stuff in. I have PP plastic bottles that had ketchup in them, they have squirty lids. I can easily sterilize water in them and then rinse out the petris. However this may be unnecessary as if I just leave them they will degrade as the bleach vinegar solution is unstable. They could also be opened and flicked with a finger to try and get droplets off. If some remain on the lid they will hopefully just stay there, and slowly degrade.

I would prefer them to be dry, I could put them on a lower power heat pad or I was thinking of putting them in my drying chamber with desiccant for a few days or a week. This may not be needed, if there is liquid still in them it will hopefully have dropped off the lids. Even if it is still has active bleach in it when you pour in agar it might seal it in down the bottom, you might have to pour agar in, let it set, and then pour another layer on top, the free liquid in the bottom should mix with the first bit of agar poured in. People do bleach dunks with cakes, so for all I know a bit of residual bleach might be beneficial.


Quote:

tump said:
I think i found the answer. Just cleaning them out, pouring grain water agar into them and dry heat sterilize then at 170 for 2.5 hours while your grain pc.


did they appear to dry out at all? if none contaminate I would take the lid off one as a control to purposely contaminate it and be sure that it will support growth.

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Invisibler.lutece
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: blackout]
    #23861913 - 11/23/16 08:54 PM (8 years, 1 month ago)

Quote:

blackout said:
Quote:

tump said:
I think i found the answer. Just cleaning them out, pouring grain water agar into them and dry heat sterilize then at 170 for 2.5 hours while your grain pc.




did they appear to dry out at all? if none contaminate I would take the lid off one as a control to purposely contaminate it and be sure that it will support growth.




I think he might have been just sterilizing empty plates with the intention of pouring later. However, if you could do no-pour in the oven, that's pretty sweet.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.

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OfflineGrundalizer
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
    #23861953 - 11/23/16 09:08 PM (8 years, 1 month ago)

Spray them with 70% ethanol and let dry in front of laminar flow hood, or sterilize under UV light.  Sterilize agar media as normal via PC.  You're good to go.

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Offlinetump
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Grundalizer]
    #23861963 - 11/23/16 09:11 PM (8 years, 1 month ago)

I did no pour in the oven. The agar doesn't look dry but i could test one by opening it.

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Invisibler.lutece
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
    #23861983 - 11/23/16 09:23 PM (8 years, 1 month ago)

Quote:

tump said:
I did no pour in the oven. The agar doesn't look dry but i could test one by opening it.




:cantarguewiththat:

Went back and reread the post. I guess it would help if I had used my eyes the first time. :\


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.

Edited by r.lutece (11/23/16 09:32 PM)

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Offlineblackout
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
    #23863792 - 11/24/16 02:16 PM (8 years, 1 month ago)

Quote:

r.lutece said:
Went back and reread the post. I guess it would help if I had used my eyes the first time. :\



In fairness I had to read it a few times myself too. And still not sure what this meant.


Quote:

tump said:
I think i found the answer. Just cleaning them out, pouring grain water agar into them and dry heat sterilize then at 170 for 2.5 hours while your grain pc.




I can only guess tump means he did this while PCing grains at the same time, but sounded like it was part of the process.

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OfflineSpore Ninja
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
    #23864185 - 11/24/16 05:47 PM (8 years, 1 month ago)

Quote:

Bigbadwooof said:
Quote:

Inocuole said:
If so, can you put your dick in it?




... I don't know, but I probably need to.




Warm agar and a Mickey's Big Mouth Bottle...

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OfflineBigbadwooof
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Spore Ninja]
    #23867828 - 11/26/16 02:55 AM (8 years, 1 month ago)

Quote:

Spore Ninja said:
Quote:

Bigbadwooof said:
Quote:

Inocuole said:
If so, can you put your dick in it?




... I don't know, but I probably need to.




Warm agar and a Mickey's Big Mouth Bottle...




How warm is warm? Should I use gentamycin? You know what? Better safe than sorry.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

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OfflineBigbadwooof
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
    #23867830 - 11/26/16 02:59 AM (8 years, 1 month ago)

I actually think you could have a high success rate by just rinsing the insides of the petris out with a rubbing alcohol spray bottle in front of a hood. Spray til alcohol drips from the surface, and washes away any possible mold spores, then allow to air dry in front of the hood. It would likely kill all of the bacterial contaminants.

Could be hit and miss, but worth a shot. We are not doing the kind of sensitive lab work that requires 100% guaranteed sterile plates. It may save some money. Rubbing alcohol is cheap where I live ($2/bottle) , and I have to wait for plates in the mail also.

I will try this at some point and report back, if nobody else does.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

Edited by Bigbadwooof (11/26/16 03:00 AM)

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