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david_polley
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Registered: 11/25/16
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Last seen: 7 years, 2 months
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how to grwo truffles and my idea
#23866316 - 11/25/16 03:15 PM (7 years, 2 months ago) |
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i used to grow magic mushrooms with great success. i have now ordered truffles spores, agar jelly and petri dishes, my plan is as follows, put the spore on agar and wait for the mycelium to grow. then sterilize alkaline mud made alkaline by adding lime to it and then sterlizing it in a pressure cooker. Then plant a hazel, ash tree stump with roots in a container. then mix the mycelium with sterile water inject into the soil and roots, put it in a fish tank indoors. Will this work? i cant find any info on the ineternet about growing truffles inside? let me know
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poofterFroth
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Registered: 03/15/14
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Re: how to grwo truffles and my idea [Re: david_polley]
#23866782 - 11/25/16 05:37 PM (7 years, 2 months ago) |
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You should start by growing your own tree seedlings and attempting to inoculate those, rather then using an existing stump/root system steady overrun with competitor fungus, spores, etc.
Transfer your truffle infected saplings to a proper outdoor growing location. Then wait ten years...
Sorry dude, but if you think you can pull off an indoor truffle grow using a fish tank yer nuts
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Edited by poofterFroth (11/25/16 06:04 PM)
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cosmicaug
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Registered: 12/17/06
Posts: 39
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Re: how to grwo truffles and my idea [Re: poofterFroth]
#23898865 - 12/06/16 02:49 AM (7 years, 1 month ago) |
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Quote:
poofterFroth said: You should start by growing your own tree seedlings and attempting to inoculate those, rather then using an existing stump/root system steady overrun with competitor fungus, spores, etc.
Transfer your truffle infected saplings to a proper outdoor growing location. Then wait ten years...
Sorry dude, but if you think you can pull off an indoor truffle grow using a fish tank yer nuts
I'd even surface sterilize the seeds and sprout them under sterile conditions before inoculating them.
Off the top of my head, I thought of maybe some plant medium (Murashige & Skooge base or maybe even dilute Miracle-Gro or similar --though I learned 3 days ago that Miracle-Gro includes a biocidal component) but then reconsidered and wondered if distilled water might be better.
Inoculate your truffle into a grain spawn (assuming that even works). After full colonization, autoclave some inert medium like vermiculite or perlite. Mix some of the fully colonized grain into your inert growing medium. Sow surface sterilized seeds into it (do something like 30 minutes in a solution of 10% household bleach followed by several rinses in sterile water for surface sterilization). As all of the nutrition will be found in the colonized grain particles, this should ensure that a mycorhyzal association forms.
Just thinking aloud. Since this is something that has been done before, he should research to see if anything is published and copy that.
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drake89
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Registered: 06/26/11
Posts: 4,168
Loc: TN
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Re: how to grwo truffles and my idea [Re: cosmicaug]
#23898972 - 12/06/16 04:58 AM (7 years, 1 month ago) |
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Quote:
cosmicaug said:
Quote:
poofterFroth said: You should start by growing your own tree seedlings and attempting to inoculate those, rather then using an existing stump/root system steady overrun with competitor fungus, spores, etc.
Transfer your truffle infected saplings to a proper outdoor growing location. Then wait ten years...
Sorry dude, but if you think you can pull off an indoor truffle grow using a fish tank yer nuts
I'd even surface sterilize the seeds and sprout them under sterile conditions before inoculating them.
Off the top of my head, I thought of maybe some plant medium (Murashige & Skooge base or maybe even dilute Miracle-Gro or similar --though I learned 3 days ago that Miracle-Gro includes a biocidal component) but then reconsidered and wondered if distilled water might be better.
Inoculate your truffle into a grain spawn (assuming that even works). After full colonization, autoclave some inert medium like vermiculite or perlite. Mix some of the fully colonized grain into your inert growing medium. Sow surface sterilized seeds into it (do something like 30 minutes in a solution of 10% household bleach followed by several rinses in sterile water for surface sterilization). As all of the nutrition will be found in the colonized grain particles, this should ensure that a mycorhyzal association forms.
Just thinking aloud. Since this is something that has been done before, he should research to see if anything is published and copy that.
people use spore slurry to inoculate commercial truffle saplings, and then do PCR (dna analysis) to determine if the correct species has infected the host tree 1 or 2 years later.
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poofterFroth
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Re: how to grwo truffles and my idea [Re: drake89]
#23899152 - 12/06/16 07:28 AM (7 years, 1 month ago) |
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Maybe a spore slurry running thru a clean hydroponic set up could be promising to infect your host saplings. But I'm not sure if the lack of other beneficial nematodes and microorganisms present in soils would have an effect on spore germination, etc.
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drake89
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Registered: 06/26/11
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Re: how to grwo truffles and my idea [Re: poofterFroth]
#23899175 - 12/06/16 07:45 AM (7 years, 1 month ago) |
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Quote:
poofterFroth said: Maybe a spore slurry running thru a clean hydroponic set up could be promising to infect your host saplings. But I'm not sure if the lack of other beneficial nematodes and microorganisms present in soils would have an effect on spore germination, etc.
They use sterile soil and incubate for 1 or 2 years in a clean room. That's what's so difficult is keeping competitor spores out for so long.
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poofterFroth
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Re: how to grwo truffles and my idea [Re: drake89]
#23899367 - 12/06/16 09:28 AM (7 years, 1 month ago) |
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What about plant tissue culture techniques? Like test tubes and nutrient mediums...
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drake89
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Registered: 06/26/11
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Re: how to grwo truffles and my idea [Re: poofterFroth]
#23899432 - 12/06/16 09:59 AM (7 years, 1 month ago) |
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Quote:
poofterFroth said:

What about plant tissue culture techniques? Like test tubes and nutrient mediums...
I don't think acorns are well suited to that. Also time, it takes up to 3 or 4 years just to propagate orchids on agar.
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poofterFroth
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Re: how to grwo truffles and my idea [Re: drake89]
#23899558 - 12/06/16 10:50 AM (7 years, 1 month ago) |
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I was kinda thinking more along the lines of cloning and rooting new growth. Maybe from an already truffle infected sapling.
It's crazy expensive to buy a certified sapling but if you could keep cloning it...
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cosmicaug
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Registered: 12/17/06
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Re: how to grwo truffles and my idea [Re: drake89]
#23902014 - 12/07/16 12:44 AM (7 years, 1 month ago) |
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Quote:
drake89 said:
Quote:
poofterFroth said:

What about plant tissue culture techniques? Like test tubes and nutrient mediums...
I don't think acorns are well suited to that. Also time, it takes up to 3 or 4 years just to propagate orchids on agar.
Orchids are very definitely a special case. I'm guessing that while orchids may have driven plant tissue culture techniques, orchids plant tissue culture timelines are definitely not typical of plant tissue culture in general. Orchids just take a long time to develop whether on agar of otherwise. They are just slow.
However, even outside of the world of orchids, I am sure that results will vary between species (given that with normal propagation techniques different plants behave differently, why would it not be the case that under sterile conditions they would also behave differently?).
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drake89
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Re: how to grwo truffles and my idea [Re: cosmicaug]
#23902386 - 12/07/16 06:58 AM (7 years, 1 month ago) |
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Quote:
cosmicaug said:
Quote:
drake89 said:
Quote:
poofterFroth said:

What about plant tissue culture techniques? Like test tubes and nutrient mediums...
I don't think acorns are well suited to that. Also time, it takes up to 3 or 4 years just to propagate orchids on agar.
(given that with normal propagation techniques different plants behave differently, why would it not be the case that under sterile conditions they would also behave differently?).
i figured if it was promising then the professionals would already be doing it. A quick google revealed nothing regarding agar propagation of oak species, other than trying to assay mycorhizae.
The reason they don't take a root cutting infected with truffle tissue and clone it, is because that's not sterile. You can't see all the hyphae/spores with your naked eye. There could be undesirable species in there undetected. A lot of species will out compete the truffle mycelium in the first year or two, which is why it's important to not allow the tree to become infected until it's planted in the field.
Edited by drake89 (12/07/16 07:12 AM)
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