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OfflineThe_breadsticks
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Registered: 09/23/16
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Worried about trich on monotubs.
    #23864428 - 11/24/16 07:16 PM (7 years, 3 months ago)

Sorry about the horrid pic quality. Some of the mycelium looks a bit too..  gray? Not white?  This is my first mono, grain done with multispore but working on getting a clean agar culture. How's she look?



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OfflineTmethylM
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Re: Worried about trich on monotubs. [Re: The_breadsticks]
    #23864499 - 11/24/16 07:40 PM (7 years, 3 months ago)

A better pic will be required for any sort of accurate diagnosis.
Grey area 'can' just be less densely colonized areas.
From this photo, however, it looks fine. :lol:


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OfflineThe_breadsticks
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Registered: 09/23/16
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Re: Worried about trich on monotubs. [Re: Tmethyl]
    #23864549 - 11/24/16 08:01 PM (7 years, 3 months ago)

I'll see what I can do. This 20 dollar phone doesn't take the best pics!!


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OfflineTmethylM
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Re: Worried about trich on monotubs. [Re: The_breadsticks]
    #23864563 - 11/24/16 08:07 PM (7 years, 3 months ago)

Shitty phone cameras are shitty because of low light situations(due to horribly small sensor). The brighter the light you can get on the substrate the clearer the photo will be, and try to stabilize your hand on something. (I'm a professional photogapher, too)


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InvisibleMudaFuka
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Re: Worried about trich on monotubs. [Re: Tmethyl]
    #23864644 - 11/24/16 08:42 PM (7 years, 3 months ago)

Looks fine from what I can see. Trich mycelium is actually brighter white than cube mycelium.


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Liquid Inoculant Tek                                   
                     

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OfflineThe_breadsticks
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Re: Worried about trich on monotubs. [Re: Tmethyl]
    #23865001 - 11/25/16 12:02 AM (7 years, 3 months ago)

Quote:

Tmethyl said:
Shitty phone cameras are shitty because of low light situations(due to horribly small sensor). The brighter the light you can get on the substrate the clearer the photo will be, and try to stabilize your hand on something. (I'm a professional photogapher, too)




Ok thanks! It's only a 1.3mp cam.. at least it's not VGA, right? Lol
I'll try again and see what i can do!!:smile:

And maybe it's not trich, maybe it's mold I keep fighting in my jars. Hmmm.... I keep ending up with a black fuzzy mold on my agar. Like today, one of thr plates I transferred from was fine. Now 6 hrs later it's got mold showing. Done inside a shmuvbox. Gahhh


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OfflineTmethylM
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Re: Worried about trich on monotubs. [Re: The_breadsticks]
    #23865009 - 11/25/16 12:11 AM (7 years, 3 months ago)

Could be a sign that your house has some mold issues if you keep getting black mold on your plates.
Aspergillus niger is a more common black mold.

Look forward to a better pic.


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OfflineThe_breadsticks
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Re: Worried about trich on monotubs. [Re: Tmethyl]
    #23865198 - 11/25/16 03:48 AM (7 years, 3 months ago)

I had wondered about that.. could it be coming from outside? I had a window AC in all summer. I'm goona spray this entire place with alcohol and Lysol!


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OfflineTmethylM
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Re: Worried about trich on monotubs. [Re: The_breadsticks]
    #23865603 - 11/25/16 10:07 AM (7 years, 3 months ago)

Looks great. I don't see any contams, possible some metabolites in pic 3.


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OfflineThe_breadsticks
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Re: Worried about trich on monotubs. [Re: Tmethyl]
    #23865682 - 11/25/16 10:38 AM (7 years, 3 months ago)

Quote:

Tmethyl said:
Looks great. I don't see any contams, possible some metabolites in pic 3.



Awesome thanks! Now if I could just get a clean agar culture.

I'll have to go UTFSE for what metabolites means.


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OfflineKillerclowns87
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Registered: 06/11/16
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Re: Worried about trich on monotubs. [Re: Tmethyl]
    #23865724 - 11/25/16 10:51 AM (7 years, 3 months ago)

Quote:

Tmethyl said:
Shitty phone cameras are shitty because of low light situations(due to horribly small sensor). The brighter the light you can get on the substrate the clearer the photo will be, and try to stabilize your hand on something. (I'm a professional photogapher, too)





Interesting! Thank you for that info!

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OfflineKillerclowns87
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Re: Worried about trich on monotubs. [Re: The_breadsticks]
    #23865730 - 11/25/16 10:54 AM (7 years, 3 months ago)

Quote:

The_breadsticks said:
Done inside a shmuvbox. Gahhh





Yea... i had good luck with the shmuvbox for a few runs then horrible a few runs. SAB seems to work much better for me. And less werk...

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InvisiblemushboyMDiscord
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Re: Worried about trich on monotubs. [Re: Killerclowns87]
    #23865743 - 11/25/16 11:01 AM (7 years, 3 months ago)

trich has a more matted, carpet kinda look. post pics of that baby pinning!

i dont know how i did anything without a SAB. oh wait. i failed a lot :p

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Invisiblefreespeech
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Re: Worried about trich on monotubs. [Re: mushboy]
    #23865756 - 11/25/16 11:07 AM (7 years, 3 months ago)

Trich mycelium upper left


Trich mycelium right side


Trich mycelium closeup

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InvisiblemushboyMDiscord
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Re: Worried about trich on monotubs. [Re: freespeech]
    #23865765 - 11/25/16 11:12 AM (7 years, 3 months ago)

nice pics. i usually do a  :breakout:
when i see it rather then snap a pic :p

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OfflinePeteyboy
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Re: Worried about trich on monotubs. [Re: mushboy]
    #23865799 - 11/25/16 11:28 AM (7 years, 3 months ago)

Quote:

mushboy said:
nice pics. i usually do a  :breakout:
when i see it rather then snap a pic :p




Hahahahhaaha:laugh2::rofl:

Freespeech: Those are amazing pics, I definitely learned from them so thank you!!!


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Edited by Peteyboy (11/25/16 11:40 AM)

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OfflineTmethylM
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Re: Worried about trich on monotubs. [Re: mushboy]
    #23866052 - 11/25/16 01:29 PM (7 years, 3 months ago)

Quote:

mushboy said:
post pics of that baby pinning!



Yes.
:fuckinawesome:


Quote:

mushboy said:
i failed a lot :p



Me too!  :cookiemonster:
It's the best way to learn.


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