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OfflineDabrit
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Registered: 12/02/15
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Can you just use a few pieces of micropore tape for GE for quart jars of colonizing WBS?
    #23864961 - 11/24/16 11:37 PM (7 years, 3 months ago)

To get to the long and short of it. I've drilled 2 x 1/4" holes in all my wide mouth quart jars. I originally intended to put RTV in one hole for direct inoculation via ms syringe and then was going to use polyfill in the other hole for GE using no synthetic or Tyvek filter underneath the lid.

Since I've realized that agar is the way to go once I'm ready to go again would there be anything wrong with say just putting a couple of pieces of micropore tape over each hole for adequate GE?

If not, why not?

Tyvek is a bitch to get in Canada unless you're buying a shit ton of the stuff. I can't find Tyvek coveralls at the department store at least any that specifically say they are made of Tyvek, and I have no idea what the postal envelopes are made out of up here so....yeah plus poly fill is a pain in the ass and I sometimes wonder if I'm stuffing it too tight for proper GE.


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OfflineTira
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Re: Can you just use a few pieces of micropore tape for GE for quart jars of colonizing WBS? [Re: Dabrit]
    #23865193 - 11/25/16 03:45 AM (7 years, 3 months ago)

It works for me, I put one layer of micropore tape on the hole inside the lid and two on the outside.  Never had any filter issues.


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OfflineTerpfreak
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Re: Can you just use a few pieces of micropore tape for GE for quart jars of colonizing WBS? [Re: Tira]
    #23865402 - 11/25/16 07:50 AM (7 years, 3 months ago)

Micropore tape is recommended over tyvek I believe.


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InvisibleMad Season
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Re: Can you just use a few pieces of micropore tape for GE for quart jars of colonizing WBS? [Re: Terpfreak]
    #23865408 - 11/25/16 07:54 AM (7 years, 3 months ago)

I used to do MP. It worked somewhat. I did 4-5 layers and my control jars didn't contaminate until a couple PC runs, so change it regularly. I've seen much better results with SFDs.


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InvisibleMudaFuka
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Re: Can you just use a few pieces of micropore tape for GE for quart jars of colonizing WBS? [Re: Mad Season]
    #23865537 - 11/25/16 09:29 AM (7 years, 3 months ago)

It works but it would be my last choice. Polyfill works great. Home Depot in Canada caries work horse brand tyvek paint suits. They don't say tyvek but they work.


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InvisibleMoabfighter
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Re: Can you just use a few pieces of micropore tape for GE for quart jars of colonizing WBS? [Re: MudaFuka]
    #23865560 - 11/25/16 09:48 AM (7 years, 3 months ago)

I have six jars going with micropore now.

Two layers before the PC, added another after inoculation

And they're colonizing in a SAB. We'll see how it goes.


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OfflineKillerclowns87
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Re: Can you just use a few pieces of micropore tape for GE for quart jars of colonizing WBS? [Re: Moabfighter]
    #23865820 - 11/25/16 11:34 AM (7 years, 3 months ago)

i Agree with tira, i do this the same way. One layer inside and 2 layers outside. Works fine for me. (But i only do this if i run out of lids with a SFD)

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OfflineSloppyJoseph
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Re: Can you just use a few pieces of micropore tape for GE for quart jars of colonizing WBS? [Re: Killerclowns87]
    #23865842 - 11/25/16 11:48 AM (7 years, 3 months ago)

Yes


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OfflineDabrit
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Re: Can you just use a few pieces of micropore tape for GE for quart jars of colonizing WBS? [Re: SloppyJoseph]
    #23866001 - 11/25/16 01:08 PM (7 years, 3 months ago)

Hells yeah.

Thanks for all the helpful responses.

Eventually I'll not be such a cheap ass and invest in synthetic filter discs.

Still very much a noob. Doing my first run with agar


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