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JCRG
Joca


Registered: 05/01/13
Posts: 13
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First time with PF - tek; lid idea
#23864902 - 11/24/16 10:57 PM (7 years, 2 months ago) |
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Welp, it's my first time growing shrooms, I'm attempting the PF tek, and I got an idea about using small syringe ports obtained from used vials at my clinic:

I sealed them with a glue pistol, first tested if it was indeed hermetic, by pouring water inside the mason jar and flipping it, checking for leaks, then obviously made the breathing ports and sealed them with micropore tape I also managed to snatch, lel


Made one with extra cotton to see if it makes a difference in my case:


And I also added another micropore layer on top:

I double sealed them with aluminium:

I prepped a small pot I used a lot to make canna butter and ayahuasca in my room ahaha, using rolled tin foil, to avoid direct contact between the jars and the floor of the pot:

I can barely fit two though:


It's an electric stove too, so I'm hoping that helps to control more evenly the temps

So now I'm gonna steam sterilize them, or maybe tyndallize, idk, depends on my patience I guess.
I hope it works though!
Edited by JCRG (11/24/16 11:42 PM)
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JCRG
Joca


Registered: 05/01/13
Posts: 13
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Re: First time with PF - tek; lid idea [Re: JCRG]
#23864903 - 11/24/16 10:57 PM (7 years, 2 months ago) |
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Ehh, I thought the Shroomery board would automatically resize these huge images, blegh.
Edit: Fixed the sizing.
Edited by JCRG (11/24/16 11:42 PM)
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: First time with PF - tek; lid idea [Re: JCRG]
#23864910 - 11/24/16 11:02 PM (7 years, 2 months ago) |
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I would stick to the tried and true method of the pf tek. As long as you have a dry verm layer on top that acts as your filter and the holes drilled are for ge.
-------------------- Keep your feet on the ground and your head in the clouds
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JCRG
Joca


Registered: 05/01/13
Posts: 13
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Quote:
Shineonyoucrazy said: I would stick to the tried and true method of the pf tek. As long as you have a dry verm layer on top that acts as your filter and the holes drilled are for ge.
Of course I didn't skip such part! I don't wanna take any chances tbh.
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: First time with PF - tek; lid idea [Re: JCRG]
#23864948 - 11/24/16 11:29 PM (7 years, 2 months ago) |
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Everything else is just unnecessary. I'd pull the micropore tape and cotton so it can get good ge during colonization. If you haven't yet i also recommend building a sab to do your work in.
-------------------- Keep your feet on the ground and your head in the clouds
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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If you already pc'd those jars roll with them, otherwise just stick to the tek. make sure your sterile procedure is good and you should have no issues. Pf cakes are easy as long as you follow the tek to the t. If you make anymore jars just punch or drill 4 holes in the top and you'll be fine
-------------------- Keep your feet on the ground and your head in the clouds
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JCRG
Joca


Registered: 05/01/13
Posts: 13
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Quote:
Shineonyoucrazy said: If you already pc'd those jars roll with them, otherwise just stick to the tek. make sure your sterile procedure is good and you should have no issues. Pf cakes are easy as long as you follow the tek to the t. If you make anymore jars just punch or drill 4 holes in the top and you'll be fine
Dude, I've put CVCs in patients, extracted 2 gallbladders and one appendix in the past, I can assure you I know how "sterile" works.
I wanna prove to myself is these crazy ideas of mine work to reduce to a minimum the chance of contams with what little I have available with me in my room, as I am currently living with sensible/conservative folks; am planning to let them sit for a while after the tyndallization and see if I can later attempt WBS for bulk/etc.
Edited by JCRG (11/24/16 11:56 PM)
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: First time with PF - tek; lid idea [Re: JCRG]
#23864991 - 11/24/16 11:55 PM (7 years, 2 months ago) |
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Do whatever you want. It's your grow
-------------------- Keep your feet on the ground and your head in the clouds
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2shoes
The anti-agar



Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB
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Re: First time with PF - tek; lid idea [Re: JCRG]
#23865236 - 11/25/16 05:00 AM (7 years, 2 months ago) |
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Quote:
JCRG said:
Quote:
Shineonyoucrazy said: If you already pc'd those jars roll with them, otherwise just stick to the tek. make sure your sterile procedure is good and you should have no issues. Pf cakes are easy as long as you follow the tek to the t. If you make anymore jars just punch or drill 4 holes in the top and you'll be fine
Dude, I've put CVCs in patients, extracted 2 gallbladders and one appendix in the past, I can assure you I know how "sterile" works.
I wanna prove to myself is these crazy ideas of mine work to reduce to a minimum the chance of contams with what little I have available with me in my room, as I am currently living with sensible/conservative folks; am planning to let them sit for a while after the tyndallization and see if I can later attempt WBS for bulk/etc.
Your ideas aren't crazy more like ghetto versions of what folks around here do anyways. The jars below are using cellulose filter discs as well as self healing injector ports.

Tyndallization is not at all what we want in this hobby.
Buy a PC!!
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wild child
Stranger
Registered: 08/06/11
Posts: 122
Last seen: 5 years, 7 months
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Re: First time with PF - tek; lid idea [Re: 2shoes]
#23865435 - 11/25/16 08:10 AM (7 years, 2 months ago) |
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the pot lid has a steamer vent, mine also does...wonder if that has any affect on the sterilizing of jars. been getting bacterial issues every time.
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: First time with PF - tek; lid idea [Re: 2shoes]
#23865520 - 11/25/16 09:16 AM (7 years, 2 months ago) |
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Quote:
2shoes said:
Quote:
JCRG said:
Quote:
Shineonyoucrazy said: If you already pc'd those jars roll with them, otherwise just stick to the tek. make sure your sterile procedure is good and you should have no issues. Pf cakes are easy as long as you follow the tek to the t. If you make anymore jars just punch or drill 4 holes in the top and you'll be fine
Dude, I've put CVCs in patients, extracted 2 gallbladders and one appendix in the past, I can assure you I know how "sterile" works.
I wanna prove to myself is these crazy ideas of mine work to reduce to a minimum the chance of contams with what little I have available with me in my room, as I am currently living with sensible/conservative folks; am planning to let them sit for a while after the tyndallization and see if I can later attempt WBS for bulk/etc.
Your ideas aren't crazy more like ghetto versions of what folks around here do anyways. The jars below are using cellulose filter discs as well as self healing injector ports.

Tyndallization is not at all what we want in this hobby.
Buy a PC!!
In his defense, he's talking about pf tek. While tyndallization (boiling for 15 minutes three days in a row) isn't how we recommend doing steam sterilization (boiling for 90 minutes in one sitting), I would think it would work for pf jars.
So if he actually did tyndallize then instead of steam sterilizing normally, I don't think it would be worth throwing them out if they're already noc'd up, but it would behoove you, JCRG, to sterilize in a tried and tested way specific to this hobby on your next go. If you move on from pf Tek, you're going to need a PC.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: First time with PF - tek; lid idea [Re: r.lutece]
#23865770 - 11/25/16 11:14 AM (7 years, 2 months ago) |
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I realize he's doing the pf tek, that's why i said all that is unnessary. Lids with a ship and sfd's (or in his case micropore tape) are more suited to grains. That's why i just recommended the old tried and true methods outlined in the good old pf tek. Ive never had any issues when i stuck to the tek. As for tantalizing why not make it an hour and a half steam and get it in in one day. You wanna get those jars going asap. Just my 2 cents.
-------------------- Keep your feet on the ground and your head in the clouds
Edited by Shineonyoucrazy (11/25/16 11:17 AM)
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JCRG
Joca


Registered: 05/01/13
Posts: 13
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Quote:
r.lutece said:
Quote:
2shoes said:
Quote:
JCRG said:
Quote:
Shineonyoucrazy said: If you already pc'd those jars roll with them, otherwise just stick to the tek. make sure your sterile procedure is good and you should have no issues. Pf cakes are easy as long as you follow the tek to the t. If you make anymore jars just punch or drill 4 holes in the top and you'll be fine
Dude, I've put CVCs in patients, extracted 2 gallbladders and one appendix in the past, I can assure you I know how "sterile" works.
I wanna prove to myself is these crazy ideas of mine work to reduce to a minimum the chance of contams with what little I have available with me in my room, as I am currently living with sensible/conservative folks; am planning to let them sit for a while after the tyndallization and see if I can later attempt WBS for bulk/etc.
Your ideas aren't crazy more like ghetto versions of what folks around here do anyways. The jars below are using cellulose filter discs as well as self healing injector ports.

Tyndallization is not at all what we want in this hobby.
Buy a PC!!
In his defense, he's talking about pf tek. While tyndallization (boiling for 15 minutes three days in a row) isn't how we recommend doing steam sterilization (boiling for 90 minutes in one sitting), I would think it would work for pf jars.
So if he actually did tyndallize then instead of steam sterilizing normally, I don't think it would be worth throwing them out if they're already noc'd up, but it would behoove you, JCRG, to sterilize in a tried and tested way specific to this hobby on your next go. If you move on from pf Tek, you're going to need a PC.
Quote:
Shineonyoucrazy said: I realize he's doing the pf tek, that's why i said all that is unnessary. Lids with a ship and sfd's (or in his case micropore tape) are more suited to grains. That's why i just recommended the old tried and true methods outlined in the good old pf tek. Ive never had any issues when i stuck to the tek. As for tantalizing why not make it an hour and a half steam and get it in in one day. You wanna get those jars going asap. Just my 2 cents.
Actually, I'm boiling them for three hours three days in a row. And I didn't know tyndallization used as little as 15 minutes lol.
Also, I really don't know if I could properly use a PC with such little electric stove tbqh.
Hopefully the 3x3 boilings will work!
Cheers everyone.
Edited by JCRG (11/25/16 09:41 PM)
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: First time with PF - tek; lid idea [Re: JCRG]
#23867652 - 11/25/16 11:46 PM (7 years, 2 months ago) |
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I'm not saying it's not unnecessary, it absolutely is. (Especially 3x3, way overkill. 15 minutes at boiling temps is the time I've always heard about for tyndallziation, so maybe a little more time each cycle to have the core matter reach temperature.) A single session of 1.5 hours is plenty of time in the pot and will probably yield better results.
What's the wattage one that stove? Anything 1500W or higher should be able to maintain a boil on a PC, and while you might not be able to toss a 30qt monster on there, it could probably handle the weight of a 15-20qt PC.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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JCRG
Joca


Registered: 05/01/13
Posts: 13
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Re: First time with PF - tek; lid idea [Re: r.lutece]
#23867698 - 11/26/16 12:18 AM (7 years, 2 months ago) |
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Quote:
r.lutece said: I'm not saying it's not unnecessary, it absolutely is. (Especially 3x3, way overkill. 15 minutes at boiling temps is the time I've always heard about for tyndallziation, so maybe a little more time each cycle to have the core matter reach temperature.) A single session of 1.5 hours is plenty of time in the pot and will probably yield better results.
What's the wattage one that stove? Anything 1500W or higher should be able to maintain a boil on a PC, and while you might not be able to toss a 30qt monster on there, it could probably handle the weight of a 15-20qt PC.
At my local walmart I've seen many small pressure cookers, however they are mostly capped to work at 80kpa (11 psi) and have no temperature/pressure measuring. Sterilization is supposed to happen with 120°C at 100kpa (15 psi) at 20 min; I know I can reach a sterilization status with fewer temps/kpa's by extending the time of the process, but given that I have no way of telling when and if such 80kpa's are reached, nor the internal temps, I'm a bit reluctant to try them, mostly out of safety.
I believe it would be easier to have a pot with a transparent lid that enables the user to visualize if the water is boiling and/or just steaming.
But then again I'm just a newb at this shrooming thingy, I just don't know what is the most prudent/stealthy way to proceed, because, as I said before, I live with conservative/sensible folks, and I do not wish to cause a stir inside the house.
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JCRG
Joca


Registered: 05/01/13
Posts: 13
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Re: First time with PF - tek; lid idea [Re: JCRG]
#23867699 - 11/26/16 12:18 AM (7 years, 2 months ago) |
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Still, thanks for all the input guys!
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JCRG
Joca


Registered: 05/01/13
Posts: 13
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Re: First time with PF - tek; lid idea [Re: JCRG]
#23871915 - 11/27/16 12:29 PM (7 years, 2 months ago) |
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Here's a small update guys, I think there might be a bit of mycelium going on in the jars:
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