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OfflineFerather
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How to make antibacterial grains * 1
    #23864026 - 11/24/16 04:13 PM (7 years, 2 months ago)

500g: Boiling hot water, 12g: Black tea. You will also need lime powder.

Make the tea as normal, squeeze using a fork for 5 minutes.
Microwave for 2 minutes and repeat both processes.

Add powdered lime up to Ph 6, no higher.
Cooked rice Ph is around 6.2 - 6.8.



Soak and then cook.


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Re: How to make antibacterial grains [Re: Ferather]
    #23864042 - 11/24/16 04:19 PM (7 years, 2 months ago)

Care to elaborate?


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OfflineFerather
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Re: How to make antibacterial grains [Re: Bunji Fungi]
    #23864051 - 11/24/16 04:23 PM (7 years, 2 months ago)

Sure, which part needs more light?


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Re: How to make antibacterial grains [Re: Ferather]
    #23864056 - 11/24/16 04:25 PM (7 years, 2 months ago)

I am curious as to why this works? The black tea and all. Does it add more nutrients or how does the tea make the grains antibacterial? Looks cool just wondering the why and how it works!


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OfflineFerather
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Re: How to make antibacterial grains [Re: Bunji Fungi]
    #23864075 - 11/24/16 04:36 PM (7 years, 2 months ago)

Oh no problem feel free to ask away. I am guessing the presence of bacillus cereus in grains is common knowledge?
Not that it is an issue if you do not know that. There are 4 active antibacterial ingredients in tea.

Two of which are active against bacillus cereus, and two more to boot.

Catechins, Tannins, Theaflavins, Theanine.


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OfflinePsilosopherr
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Re: How to make antibacterial grains [Re: Ferather]
    #23864080 - 11/24/16 04:40 PM (7 years, 2 months ago)

drops mic on stage...what will he think of next?

I remember green tea having similar properties, perhaps use both. I'll have to try this


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OfflineFerather
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Re: How to make antibacterial grains [Re: Psilosopherr]
    #23864088 - 11/24/16 04:42 PM (7 years, 2 months ago)

Green tea has also the same properties, however the ratio is different. I haven't tried it though sorry.


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Re: How to make antibacterial grains [Re: Ferather]
    #23864180 - 11/24/16 05:42 PM (7 years, 2 months ago)

Definitely awesome, hadn't thought about adding antibacterial to the soak. Saving this for later. :thumbup:


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Re: How to make antibacterial grains [Re: Ferather] * 1
    #23864181 - 11/24/16 05:43 PM (7 years, 2 months ago)

Black tea is also antifungal.


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OfflineLeftfield420
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Re: How to make antibacterial grains [Re: Ferather]
    #23864230 - 11/24/16 06:06 PM (7 years, 2 months ago)

Is there slower growth with tea soaked grains? as stated with the tea agar I read about earlier?


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InvisibleJosex
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Re: How to make antibacterial grains [Re: Leftfield420]
    #23864248 - 11/24/16 06:13 PM (7 years, 2 months ago)

:threadmonitor:


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OfflinePeteyboy
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Re: How to make antibacterial grains [Re: Josex]
    #23864280 - 11/24/16 06:24 PM (7 years, 2 months ago)

:youthemandawg:


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OfflinecronicrFacebook
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Re: How to make antibacterial grains [Re: Peteyboy]
    #23864291 - 11/24/16 06:28 PM (7 years, 2 months ago)

So what no pc no nothing?


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Re: How to make antibacterial grains [Re: cronicr]
    #23864321 - 11/24/16 06:39 PM (7 years, 2 months ago)

so you use this tea water for your grains and such?

does anti fungal proprieties of black tea translate at all to cubes? or edibles?
i dont know the science of it att all.  :confused:



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Re: How to make antibacterial grains [Re: Munchauzen]
    #23864368 - 11/24/16 06:59 PM (7 years, 2 months ago)

Myc should still colonize it. Spores just won't germ.
Mold will still grow on it, most fungicide prevents spore germination.
RR claims to be able to open air colonize grains with fungicide.
Do you have any pictures of tubs grown with this method?
How do they compare to regular good sterile technique?


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Re: How to make antibacterial grains [Re: ComebackKid]
    #23864411 - 11/24/16 07:12 PM (7 years, 2 months ago)

Another question: if the tea is fungicidal enough to prevent mold spores from germinating, is it something you could consider misting with as opposed to regular water? Or dunking?


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Re: How to make antibacterial grains [Re: r.lutece]
    #23864654 - 11/24/16 08:48 PM (7 years, 2 months ago)

I somtimes hydrate my casing layers with fungicide to keep trich from germinating.


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Re: How to make antibacterial grains [Re: MudaFuka]
    #23864662 - 11/24/16 08:53 PM (7 years, 2 months ago)

:popcorn:


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OfflinePinPornProducer
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Re: How to make antibacterial grains [Re: Josex]
    #23864666 - 11/24/16 08:55 PM (7 years, 2 months ago)

Quote:

Josex said:
:threadmonitor:



:whathesaid:


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Offlinetump
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Re: How to make antibacterial grains [Re: r.lutece]
    #23864682 - 11/24/16 09:03 PM (7 years, 2 months ago)

Interesting


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Re: How to make antibacterial grains [Re: tump]
    #23864692 - 11/24/16 09:06 PM (7 years, 2 months ago)

Banrot has been successfully used in casings and grains.


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Invisiblemorty422
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Re: How to make antibacterial grains [Re: cronicr]
    #23864826 - 11/24/16 10:09 PM (7 years, 2 months ago)

Quote:

cronicr said:
Banrot has been successfully used in casings and grains.





Tea time, eh?

:threadmonitor:


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Re: How to make antibacterial grains [Re: morty422]
    #23864841 - 11/24/16 10:16 PM (7 years, 2 months ago)

VDG was working with benomyl to prevent trich germinating.


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InvisibleMudaFuka
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Re: How to make antibacterial grains [Re: Pastywhyte] * 1
    #23864851 - 11/24/16 10:20 PM (7 years, 2 months ago)

I use tough actin Tinactin.


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OfflineWeavieWonder
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Re: How to make antibacterial grains [Re: MudaFuka] * 1
    #23864892 - 11/24/16 10:47 PM (7 years, 2 months ago)

So is this something you theorized while blazing a fatty, or do you have scienfific data and experiential evidence to back this up?  My balls itched like a mother fucker one time, so I dipped them in hot tea, and blamo, no more ball itch.  Thank you tea for solving all my life problems.


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OfflineFerather
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Re: How to make antibacterial grains [Re: r.lutece]
    #23865273 - 11/25/16 05:51 AM (7 years, 2 months ago)

Quote:

cronicr said:
So what no pc no nothing?



Sorry this write up is for the soak, you soak as normal, then pressure cook as normal.




Quote:

Munchauzen said:
Black tea is also antifungal.



The fungicide present in tea is also produced by mycelium to reduce sporulation.




Quote:

Leftfield420 said:
Is there slower growth with tea soaked grains? as stated with the tea agar I read about earlier?



There will be a small difference in speed with a high starch ratio.




Quote:

mushboy said:
So you use this tea water for your grains and such?

Does anti fungal proprieties of black tea translate at all to cubes? or edibles?
I dont know the science of it at all.  :confused:





The same across the board for all commonly grown types, both active and non-active.




Quote:

r.lutece said:
Another question: if the tea is fungicidal enough to prevent mold spores from germinating, is it something you could consider misting with as opposed to regular water? Or dunking?



You could try that, and the casing with added tea idea is looking clever.


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Offlinetump
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Re: How to make antibacterial grains [Re: Ferather]
    #23865288 - 11/25/16 06:09 AM (7 years, 2 months ago)

But that logic wouldn't making lc with malt and tea if knocked up by agar be unstoppable. This idea with grain makes sense. Next batch for sure


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OfflineFerather
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Re: How to make antibacterial grains [Re: tump]
    #23865291 - 11/25/16 06:14 AM (7 years, 2 months ago)

I think katbusa did some tests with tea LC or LI, not sure what the result was.


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Re: How to make antibacterial grains [Re: Ferather]
    #23865294 - 11/25/16 06:19 AM (7 years, 2 months ago)

this is a cool idea.

what the story with the lime?  what happened when u didn't add it?


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InvisibleTeemo 6T3
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Re: How to make antibacterial grains [Re: blindingleaf]
    #23865305 - 11/25/16 06:25 AM (7 years, 2 months ago)

:whathesaid:

I first thought he was using lime cus tea water was alkaline, but it seems tea is actually acidic.


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OfflineFerather
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Re: How to make antibacterial grains [Re: blindingleaf]
    #23865306 - 11/25/16 06:26 AM (7 years, 2 months ago)

Ph will be too low, which will reduce growth speed and cause breakdown on grains.
Lime is also considered lightly antibacterial, so more to boot.


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Invisibleblindingleaf
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Re: How to make antibacterial grains [Re: Ferather]
    #23865316 - 11/25/16 06:32 AM (7 years, 2 months ago)

what happens if ur grains burst during the PC cycle?  is the recipe still as preventative?


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OfflineFerather
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Re: How to make antibacterial grains [Re: Ferather]
    #23865319 - 11/25/16 06:35 AM (7 years, 2 months ago)

More to note, going over Ph 6 will destabilize the active antibacterial ingredients.
Cooked rice Ph is 6.2 - 6.8, so Ph 6 - 6.2 is perfect across the board.

I live in a hard water area, and my water Ph is 8.2 - 8.4.
You will need to balance Ph manually, sorry.


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OfflineFerather
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Re: How to make antibacterial grains [Re: blindingleaf]
    #23865321 - 11/25/16 06:38 AM (7 years, 2 months ago)

Quote:

blindingleaf said:
what happens if ur grains burst during the PC cycle?  is the recipe still as preventative?



Technically you would absorb more in the middle of the grain, any cooking will reduce the effects.
To answer, yes it will still work, as long as the end result suits its intended purpose.


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OfflineFerather
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Re: How to make antibacterial grains [Re: Ferather]
    #23865369 - 11/25/16 07:20 AM (7 years, 2 months ago)



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Re: How to make antibacterial grains [Re: Ferather]
    #23865401 - 11/25/16 07:50 AM (7 years, 2 months ago)

Need to try this... but I can totally see me ruining a run somehow by deviating from my normal routine lol


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OfflineFerather
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Re: How to make antibacterial grains [Re: Thedenthead]
    #23865416 - 11/25/16 08:00 AM (7 years, 2 months ago)

Well my advice would be make 1 or 2 small samples to play with on the side.
You could even throw the tea leaves in for a bit of extra looseness.

A digital Ph pen is about £6, check Amazon or eBay.


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Re: How to make antibacterial grains [Re: Ferather]
    #23865568 - 11/25/16 09:51 AM (7 years, 2 months ago)

:threadmonitor:


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OfflineFerather
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Re: How to make antibacterial grains [Re: wolfedawwg]
    #23865628 - 11/25/16 10:18 AM (7 years, 2 months ago)

36 Natural Alternatives for Infection, some listed are very cheap and easy to obtain.
I suggest the agar test method and Ph 6 - 6.4 via lime not gypsum.


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Re: How to make antibacterial grains [Re: Ferather]
    #23865813 - 11/25/16 11:32 AM (7 years, 2 months ago)

I'd love to see some more info on this. No matter what I do I always seem to have a little bacteria in my spawn.  I can't see it but it makes my jars smell a little musty


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Re: How to make antibacterial grains [Re: Kenetic]
    #23865920 - 11/25/16 12:29 PM (7 years, 2 months ago)

Quote:

kenetic said:
I'd love to see some more info on this. No matter what I do I always seem to have a little bacteria in my spawn.  I can't see it but it makes my jars smell a little musty



PC about 60g of rice, inoculate with spores, transfer to T-Agar or T-Gel balanced to Ph 6.
Then isolate a clean section about 28mm away from the spawn sample.

Pass to tea infused grains or normal grains as spawn.

Mycolorado's actives testing here.


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Re: How to make antibacterial grains [Re: Ferather]
    #23866114 - 11/25/16 01:57 PM (7 years, 2 months ago)

:goodmorning:


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OfflineTyrannical Justice
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Re: How to make antibacterial grains [Re: Marty Mycfly]
    #23866182 - 11/25/16 02:25 PM (7 years, 2 months ago)

Im definitely having a go at this one. I can never get my grains just right so this could be the key to me succeeding.


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Re: How to make antibacterial grains [Re: Tyrannical Justice]
    #23866763 - 11/25/16 05:32 PM (7 years, 2 months ago)

what's wrong with low ph for cube myc? only problem with low pH is that lower fungi loves it, but those cant handle higher pH, thats why we lime casing layers.


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Re: How to make antibacterial grains [Re: spacechildo] * 1
    #23867072 - 11/25/16 07:18 PM (7 years, 2 months ago)

Im with kenetic i have bacteria issues as well this is interesting


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Re: How to make antibacterial grains [Re: Boogieman47]
    #23867483 - 11/25/16 10:18 PM (7 years, 2 months ago)

interesting :strokebeard:

So this method u cannot innocate with spores? Has to be a live culture to the anti-bact grains?


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Re: How to make antibacterial grains [Re: LogicaL Chaos]
    #23867655 - 11/25/16 11:47 PM (7 years, 2 months ago)

Sounds just like the way gentamicin sulfate kills fungal spores, including cubensis spores. Strictly speaking they CAN germinate on antibacterial agar, but it's much less likely than standard agar.


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Re: How to make antibacterial grains [Re: LogicaL Chaos]
    #23867891 - 11/26/16 05:03 AM (7 years, 2 months ago)

Correct


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Re: How to make antibacterial grains [Re: r.lutece]
    #23868003 - 11/26/16 07:15 AM (7 years, 2 months ago)

Quote:

spacechildo said:
what's wrong with low ph for cube myc? only problem with low pH is that lower fungi loves it, but those cant handle higher pH, thats why we lime casing layers.



The lower Ph T-Gel test I did, increased bacterial activity for 2 days. Ph 6 did the opposite, no activity.


Quote:

r.lutece said:
Sounds just like the way gentamicin sulfate kills fungal spores, including cubensis spores. Strictly speaking they CAN germinate on antibacterial agar, but it's much less likely than standard agar.



Agreed. You can germinate mold on almost any "open air safe" grow, its just very low in chance.

Mold germinates very well on the remaining tea waste leaves, solubles removed.


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Re: How to make antibacterial grains [Re: MudaFuka]
    #23868008 - 11/26/16 07:19 AM (7 years, 2 months ago)

Quote:

MudaFuka said:
I use tough actin Tinactin.




John Mudafukin Madden


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Re: How to make antibacterial grains [Re: Inocuole]
    #23868150 - 11/26/16 08:23 AM (7 years, 2 months ago)

Here we have accidental germination of Golden oyster spores after 2.8 days.
Paper, enriched with tea leaves and fertilizer, 88% water content.

   

Very strong, very radial, uniform growth.


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Re: How to make antibacterial grains [Re: Ferather]
    #23868177 - 11/26/16 08:34 AM (7 years, 2 months ago)

kinda sorta related: I am doing side by side P. mexicana Jalisco truffles, one ysed a 50:50 coffee:water ratio and one 50:50 tea:water. The coffee is laying waste to the tea, though the tea is still colonizing.


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Re: How to make antibacterial grains [Re: PlantManBee]
    #23868197 - 11/26/16 08:40 AM (7 years, 2 months ago)

Lots of starch in coffee, its kinda like roasted rye berry's. No starch in tea, not so comparable.
Still I am interested on how well that coffee does given it has been highly roasted.

Which is good for being clean to start with, bad for nutrition.


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Re: How to make antibacterial grains [Re: Ferather]
    #23868231 - 11/26/16 08:49 AM (7 years, 2 months ago)

Quote:

Ferather said:
Lots of starch in coffee, its kinda like roasted rye berry's. No starch in tea, not so comparable.
Still I am interested on how well that coffee does given it has been highly roasted.

Which is good for being clean to start with, bad for nutrition.



Bad for nutrition? Coffee is a nitrogen powerhouse and molds fast as all fuck left liquid or grounds


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Re: How to make antibacterial grains [Re: bodhisatta]
    #23868245 - 11/26/16 08:54 AM (7 years, 2 months ago)

I don't disagree with it working, because it is widely used.
Still what happens if you roast rye until black?


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Re: How to make antibacterial grains [Re: Ferather]
    #23868279 - 11/26/16 09:06 AM (7 years, 2 months ago)

Tastes like shit. Still plenty of nutrition. It doesn't evaporate off. Cubensis may not prefer if blackened but most other things couldn't give a shit. Macronutrient may be broken down sure but that doesn't matter much to most microorganisms. PH may change too but also not a big game changing thing with fungi


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Re: How to make antibacterial grains [Re: Ferather]
    #23868314 - 11/26/16 09:20 AM (7 years, 2 months ago)

Mmmmm...i am dubious as to high starch in coffee. Comparatively sure. But it still has VERY few calories, of which a fairly high percentage is protein .

EDIT: 2 calories for an 8 oz cup. So starch and sugars.


Edited by PlantManBee (11/26/16 09:33 AM)


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Re: How to make antibacterial grains [Re: PlantManBee]
    #23868336 - 11/26/16 09:26 AM (7 years, 2 months ago)

Fair enough bodhisatta, as long as my point was recognized correctly. No argument here on usefulness.

@PlantManBee, There is 28.5g of starch and 14.6g of protein per 100g dry roasted coffee.


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Re: How to make antibacterial grains [Re: Ferather]
    #23868363 - 11/26/16 09:34 AM (7 years, 2 months ago)

We are using brewed coffee, correct? It's the water extracted values that are going to apply iiuc


Edited by PlantManBee (11/26/16 09:38 AM)


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Re: How to make antibacterial grains [Re: PlantManBee]
    #23868370 - 11/26/16 09:36 AM (7 years, 2 months ago)

No offence but you are comparing brewed tea to brewed coffee, so you still have starch in the coffee as a difference.
The other thing is are you using the leaves alongside, because coffee is fully soluble in comparison.


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Re: How to make antibacterial grains [Re: Ferather]
    #23868381 - 11/26/16 09:42 AM (7 years, 2 months ago)

Lol im just going for clarity and understanding. Not trying to dump your cart. Peace


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Re: How to make antibacterial grains [Re: PlantManBee]
    #23868387 - 11/26/16 09:47 AM (7 years, 2 months ago)

Sorry I am not trying to be rude, I just don't think it is a fair comparison, take no offence.


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Re: How to make antibacterial grains [Re: Ferather]
    #23868539 - 11/26/16 10:46 AM (7 years, 2 months ago)

2 calories of starch vs. 2 calories of starch (likely starch, the caloric values are not separated in any value tables i've come across)  is NOT a difference at all. Evidence suggests there is not an appreciable difference in starch content. That is just what i see. If you can tell me why i am misinterpreting the information i would love that.

Other nutrients like minerals are going to have much larger differences between the two than the starch content. Caffeine and tannins are likely WAY different between the two, but starch is practically nonexistent in the brewed beverages unless you add some.

BTW, I DO have lot's of biology credits under my belt. Again, clarity and is my goal regarding the organisms we're playing with. Good work so far! I mean that! :stoned:


Edited by PlantManBee (11/26/16 12:33 PM)


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Re: How to make antibacterial grains [Re: PlantManBee] * 3
    #23868594 - 11/26/16 11:04 AM (7 years, 2 months ago)

This thread is full of some of the hardest reaching I've seen in a while.


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Re: How to make antibacterial grains [Re: PlantManBee]
    #23868597 - 11/26/16 11:04 AM (7 years, 2 months ago)

You will still need to use all of the tea to be comparable.
Try Tea Granules, tempted to try it myself.


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Re: How to make antibacterial grains [Re: Ferather] * 1
    #23868698 - 11/26/16 11:32 AM (7 years, 2 months ago)

for what it's worth every beer I've made with tea had counts come up when it was plated for QA. that's tea not added during the boil. it's tea steeped at 185-212F (depending on the tea) and then injected into the bright beer tanks. obviously it's way more diluted. but despite the antibacterial action of hops the tea still made beers get counts of bacteria.

I suppose I wouldn't call this antibacterial grains, it would be inhibited bacteria grains. with some component of the tea'ing being somewhat antibacterial


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Re: How to make antibacterial grains [Re: Inocuole] * 2
    #23868755 - 11/26/16 11:50 AM (7 years, 2 months ago)

I'm not 100% positive what that means exactly. But for some reason I can't help but agree...
:resignednonacceptance:
There seems to be a lot of empty claims


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Re: How to make antibacterial grains [Re: ComebackKid]
    #23868773 - 11/26/16 11:59 AM (7 years, 2 months ago)

Quote:

ComebackKid said:
I'm not 100% positive what that means exactly. But for some reason I can't help but agree...
:resignednonacceptance:
There seems to be a lot of empty claims



Agreeing with what you don't understand...


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Re: How to make antibacterial grains [Re: Mycolorado] * 1
    #23868781 - 11/26/16 12:02 PM (7 years, 2 months ago)

English might not be his first language..  Some colloquialisms sound weird as fuck and don't make sense. He seemed to get what I was talking about though.


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Re: How to make antibacterial grains [Re: ComebackKid] * 1
    #23868790 - 11/26/16 12:04 PM (7 years, 2 months ago)

Quote:

Ferather said:
There seems to be a lot of empty claims




this guy gets it, no doubt!


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Re: How to make antibacterial grains [Re: Inocuole]
    #23868791 - 11/26/16 12:04 PM (7 years, 2 months ago)

Just fucking with comeback.


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Re: How to make antibacterial grains [Re: Inocuole]
    #23868859 - 11/26/16 12:29 PM (7 years, 2 months ago)

Haha no I just have vocabulary issues sometimes. I find it hard to words, but I try. :justdontknow:
I also grow a big rubbery one anytime I see words like colloquialisms being used in appropriate situations.
I'm very thankful to live in a time where I can look up big fancy words in the dictionary at any given time :lol:


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Re: How to make antibacterial grains [Re: ComebackKid]
    #23868865 - 11/26/16 12:31 PM (7 years, 2 months ago)

double click, right click, search google for... et voila! being foreign (on the internet) has never been as easy as it is today. :tongue2:


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Re: How to make antibacterial grains [Re: spacechildo]
    #23868877 - 11/26/16 12:34 PM (7 years, 2 months ago)

Did someone mention global shift in conciousness?


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Re: How to make antibacterial grains [Re: ComebackKid]
    #23868887 - 11/26/16 12:37 PM (7 years, 2 months ago)

Quote:

ComebackKid said:
Haha no I just have vocabulary issues sometimes. I find it hard to words, but I try. :justdontknow:
I also grow a big rubbery one anytime I see words like colloquialisms being used in appropriate situations.
I'm very thankful to live in a time where I can look up big fancy words in the dictionary at any given time :lol:




Ah, yeah I didn't think you sounded particularly foreign or anything but I do work with a lot of international people and there's a lot of shit I can't expect them to understand when I say.


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Re: How to make antibacterial grains [Re: spacechildo]
    #23868895 - 11/26/16 12:40 PM (7 years, 2 months ago)

Fancy words CAN be helpful... or not. Sorry, I still use words professors like because it can help there. I think there are some conclusions being jumped to here, but if it's working for some reason, I'd like to figure out why. :wink:


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Re: How to make antibacterial grains [Re: PlantManBee] * 1
    #23868914 - 11/26/16 12:45 PM (7 years, 2 months ago)

I like to use idioms and salty vernacular as much as the next guy but sometimes the proper nomenclature is the only way to adequately explain what needs to be expressed.


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Re: How to make antibacterial grains [Re: Inocuole]
    #23868927 - 11/26/16 12:48 PM (7 years, 2 months ago)

since this thread is already off the tracks, I've always been curious about how people end up on your hall of shame inocuole.


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Re: How to make antibacterial grains [Re: Psilosopherr]
    #23868947 - 11/26/16 12:55 PM (7 years, 2 months ago)

on a more relevant note, perhaps those who want to be extra careful could use soapy tea to spray their SAB's down?


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Re: How to make antibacterial grains [Re: spacechildo]
    #23868965 - 11/26/16 01:02 PM (7 years, 2 months ago)

Quote:

spacechildo said:
Quote:

Ferather said:
There seems to be a lot of empty claims




this guy gets it, no doubt!




Why is my name in that quote?


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Re: How to make antibacterial grains [Re: Psilosopherr]
    #23868979 - 11/26/16 01:05 PM (7 years, 2 months ago)

Quote:

Psilosopherr said:
since this thread is already off the tracks, I've always been curious about how people end up on your hall of shame inocuole.





Usually when people click ignore after the only thing I told them was the truth.  Once that happens I start answering all their questions very comprehensively so that everybody else is like "well inoc got it covered.." and doesn't answer them, so then they think everyone else is ignoring them and end up doing goofy shit like grow in the dark for years.  Petty?  Absolutely.  Hilarious way to fuck with someone who thinks they're rid of me forever?  Even more so.


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OfflinePlantManBee
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Re: How to make antibacterial grains [Re: Psilosopherr]
    #23868985 - 11/26/16 01:06 PM (7 years, 2 months ago)

yeah, looking back even briefly i'm talking about knocking over his cart.... sorry man. I like idiomatic expressions :smirk:


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Re: How to make antibacterial grains [Re: PlantManBee]
    #23868997 - 11/26/16 01:09 PM (7 years, 2 months ago)

Right hang on, no offense but WTF is happening ATM? Got people quoting things I'm not saying and a whole bunch of crap I don't welcome.

Can you go argue irrelevant information and off topic discussions elsewhere, thanks. Ferather.


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Re: How to make antibacterial grains [Re: Ferather]
    #23869021 - 11/26/16 01:17 PM (7 years, 2 months ago)

my quote probably got fucked up because I was gonna quote and reply to you earlier, but figured I wouldn't bother,
but next time I opened this thread that quote was still in my textbox. just a small hickup.

how's your grains looking today then? to keep the thread on the tracks...


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Re: How to make antibacterial grains [Re: Pastywhyte]
    #23869079 - 11/26/16 01:36 PM (7 years, 2 months ago)

Quote:

Pastywhyte said:
I like to use idioms and salty vernacular as much as the next guy but sometimes the proper nomenclature is the only way to adequately explain what needs to be expressed.




:micdrop:

Edit: my apologies

May I suggest an open air colonization of a small amount of grain? Say a pint jar?


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Edited by ComebackKid (11/26/16 01:39 PM)


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Re: How to make antibacterial grains [Re: ComebackKid]
    #23869222 - 11/26/16 02:38 PM (7 years, 2 months ago)

I welcome any ideas, improvements, suggestions, nay say and so on, as long as comments are on topic.
Open air colonization I can do, I can also infect it with dust lying around the house.

By all means, if you have time, and are willing feel free to experiment.

Or prove me completely wrong, I don't mind  :smile:


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Re: How to make antibacterial grains [Re: Ferather]
    #23869226 - 11/26/16 02:40 PM (7 years, 2 months ago)

I would rather see you prove yourself right :shrug: got evidence?


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Re: How to make antibacterial grains [Re: bodhisatta]
    #23869235 - 11/26/16 02:42 PM (7 years, 2 months ago)

:whathesaid:
Unfortunately I got a lot on my plate right right now to be running these tests. But I got time for popcorn :popcorn:


--------------------
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Re: How to make antibacterial grains [Re: ComebackKid]
    #23869259 - 11/26/16 02:49 PM (7 years, 2 months ago)

I ran two tests at first, one I can take current images, the other one I can do again but have no current assembly.

The first test was a tea bag (3g) with 3g of grain flour and 1g fertilizer cooked for 2 minutes.
It got bacteria, but it stalled after 2 days, it is still stalled now, and visibly obvious.

The second I assembled TGF-Tek and microwaved only for 20 minutes.
I did not pressure cook, and no infection occurred at all.

The latter however needs further testing.


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Re: How to make antibacterial grains [Re: ComebackKid]
    #23869270 - 11/26/16 02:52 PM (7 years, 2 months ago)

I almost feel like running all your teks together. Put a clone in your tea-agar then cut a piece into tea/ grain water for lc . injecting into your tea-grains. Then top end it in a mono tub cased in tea casing. Ps more test i run fail


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Re: How to make antibacterial grains [Re: bodhisatta]
    #23869347 - 11/26/16 03:18 PM (7 years, 2 months ago)

Quote:

bodhisatta said:
I would rather see you prove yourself right :shrug: got evidence?




this.

its not up to us to prove your stuff wrong, when you make claims and write teks etc
its up to you to show how good it works from start to finish.

If you claim soaking grains in tea helps inhibit or even removes already growing bacteria from a culture, you need to show it with pics and back up your claims.

OP here just shows how to make black tea as far as i'm concerned :shrug:


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Re: How to make antibacterial grains [Re: spacechildo]
    #23869361 - 11/26/16 03:21 PM (7 years, 2 months ago)

"burden of proof"


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Re: How to make antibacterial grains [Re: spacechildo]
    #23869362 - 11/26/16 03:22 PM (7 years, 2 months ago)

Agreed...we tend to not like things labeled as teks without final product proof.


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Re: How to make antibacterial grains [Re: Ferather]
    #23869377 - 11/26/16 03:28 PM (7 years, 2 months ago)

Quote:

Ferather said:
Still what happens if you roast rye until black?



Still grows, well I presume it would, this was wheat which was burnt.



more info here https://www.shroomery.org/forums/showflat.php/Number/4892572

In this thread I was wondering about using ready roasted grains, which could possibly just need steaming for a successful grow. It is mainly about microwaving agar which seems to be of interest to you.

https://www.shroomery.org/forums/showflat.php/Number/20938439#20938439


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Re: How to make antibacterial grains [Re: blackout]
    #23869477 - 11/26/16 03:55 PM (7 years, 2 months ago)

posts interesting idea, says you're free to test it if you want.

Gets shit on for burden of proof. :rolleyes:

don't want to write an additional reply but I'll roll my eyes even harder at the response :whatever:


Edited by Psilosopherr (11/26/16 04:41 PM)


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Re: How to make antibacterial grains [Re: Ferather]
    #23869498 - 11/26/16 04:04 PM (7 years, 2 months ago)

I recently got 4 jars that had the black mold on it :frown: and was wondering would this prevent that if it were to happen again ?


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Re: How to make antibacterial grains [Re: tripo3]
    #23869501 - 11/26/16 04:05 PM (7 years, 2 months ago)

Doubtful. thats a sterile technique thing.


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Re: How to make antibacterial grains [Re: Psilosopherr] * 1
    #23869513 - 11/26/16 04:10 PM (7 years, 2 months ago)

Quote:

Psilosopherr said:
posts interesting idea, says you're free to test it if you want.

Gets shit on for burden of proof. :rolleyes:




name of the thread is "how to make antibacterial grains".
then he should prove it works, not wait 100 replies deep into the thread to say you can test it if you like.
its just not how shit works here. if you have an idea or crazy theory, just call your thread "crazy theory/untested idea". not TEK on how to... etc! understand what we're getting at?

now you get a bunch of newbs finding this thinking its proven shit, a new tek to help you avoid bacteria etc.

smells like bAnnies shit all over again IMO!


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Re: How to make antibacterial grains [Re: spacechildo]
    #23869559 - 11/26/16 04:24 PM (7 years, 2 months ago)

To be fair at least annie had a few pics from 1996.


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Re: How to make antibacterial grains [Re: bodhisatta]
    #23869578 - 11/26/16 04:30 PM (7 years, 2 months ago)

Quote:

bodhisatta said:
I would rather see you prove yourself right :shrug: got evidence?



This


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Re: How to make antibacterial grains [Re: TheMustardTiger]
    #23869649 - 11/26/16 04:56 PM (7 years, 2 months ago)

Quote:

TheMustardTiger said:
Quote:

bodhisatta said:
I would rather see you prove yourself right :shrug: got evidence?



This




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Re: How to make antibacterial grains [Re: Inocuole]
    #23871466 - 11/27/16 09:40 AM (7 years, 2 months ago)

Quote:

bodhisatta said:
I would rather see you prove yourself right :shrug: got evidence?



This




I have read this whole thread and while I can indeed prep grains this way, I still don't know how to make "antibacterial grains" as the title suggests....:shrug:


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Re: How to make antibacterial grains [Re: wolfedawwg] * 1
    #23871932 - 11/27/16 12:35 PM (7 years, 2 months ago)

Quote:

wolfedawwg said:
Quote:

bodhisatta said:
I would rather see you prove yourself right :shrug: got evidence?



This




I have read this whole thread and while I can indeed prep grains this way, I still don't know how to make "antibacterial grains" as the title suggests....:shrug:


pc them.


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Re: How to make antibacterial grains [Re: cronicr]
    #23872023 - 11/27/16 01:15 PM (7 years, 2 months ago)

Testing we have logged so far: here, here and here. I am not using starch in my testing, both katbusa and Mycolorado are using ME.
I did some additional testing with ratios, enrichments, and Ph. Data was highly conclusive after the Ph testing.

The correct ratio required to infuse materials with antibacterial effects was established.


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Re: How to make antibacterial grains [Re: Ferather]
    #23872048 - 11/27/16 01:25 PM (7 years, 2 months ago)

I've looked through those threads several times now but I still cant find the pics of colonizing grains or any mushrooms growing,
could you perhaps help me and show the direct link? I'm feeling kinda dumb over here.. :crazy:


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Re: How to make antibacterial grains [Re: spacechildo] * 1
    #23872105 - 11/27/16 01:42 PM (7 years, 2 months ago)

Give me some time and I will log some hard testing. I've not logged any of my light testing.
My current focus is on cleaning several samples I have, so bare with me, sorry.


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Re: How to make antibacterial grains [Re: Ferather]
    #23872118 - 11/27/16 01:52 PM (7 years, 2 months ago)

I have 4 small containers and 1kg of rye, I can do PC vs Microwave, 2 covered 2 uncovered.
I can also do enriched, Ph changed and so on, all recipe's tea infused.

Can start sometime next week, probably closer to Friday.


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Re: How to make antibacterial grains [Re: Ferather] * 1
    #23872134 - 11/27/16 01:57 PM (7 years, 2 months ago)

if you wanna show the antibacterial properties of the tea rather do some with tea some without tea. only change 1 vector at a time, dont start messing with ph,microwaves and all that stuff at once.

It'd be cool if you could take samples of bacterial cultures put to tea-grains and regular grains and show how the tea helps rid the bacteria.

that's the point of this thread, right? If I'm not completely mistaken?


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Re: How to make antibacterial grains [Re: spacechildo]
    #23872149 - 11/27/16 02:01 PM (7 years, 2 months ago)

Quote:

spacechildo said:
if you wanna show the antibacterial properties of the tea rather do some with tea some without tea. only change 1 vector at a time, dont start messing with ph,microwaves and all that stuff at once.

It'd be cool if you could take samples of bacterial cultures put to tea-grains and regular grains and show how the tea helps rid the bacteria.

that's the point of this thread, right? If I'm not completely mistaken?



Indeed. First point taken, will do. I will have to assemble something for the bacteria cultivation.
I can try and isolate some bacillus cereus from grains, bear with me then.


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Re: How to make antibacterial grains [Re: Ferather]
    #23872165 - 11/27/16 02:05 PM (7 years, 2 months ago)

you dont really need to do that, just squirt a good 1/2 - 1 cc from a spore syringe to an agar plate and you'll be sure to get at least some bacteria.

I mean, you have done this yourself and seen bacteria disappear once put to grains right? since you've already made the thread and posted the howto? or is this just guesswork based off a theory?


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Re: How to make antibacterial grains [Re: spacechildo] * 1
    #23872220 - 11/27/16 02:18 PM (7 years, 2 months ago)

I made up 4 plates with Tea/PDA mixture to see what happens myself...I have some transfers from contamed plates and I will see if it cleans them up or what?


Edited by Leftfield420 (11/27/16 02:19 PM)


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Re: How to make antibacterial grains [Re: Leftfield420] * 1
    #23872248 - 11/27/16 02:28 PM (7 years, 2 months ago)

Quote:

spacechildo said:
you dont really need to do that, just squirt a good 1/2 - 1 cc from a spore syringe to an agar plate and you'll be sure to get at least some bacteria.

I mean, you have done this yourself and seen bacteria disappear once put to grains right? since you've already made the thread and posted the howto? or is this just guesswork based off a theory?



I used infected spawn, it doesn't disappear alone, but it does stall and stay that way, mycelium over grow it.

Quote:

Leftfield420 said:
I made up 4 plates with Tea/PDA mixture to see what happens myself...I have some transfers from contamed plates and I will see if it cleans them up or what?



Yes it will, allow it to grow out and take a transfer to normal agar, to verify.


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Re: How to make antibacterial grains [Re: Ferather]
    #23872266 - 11/27/16 02:34 PM (7 years, 2 months ago)

That is the plan...


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Re: How to make antibacterial grains [Re: spacechildo]
    #23872407 - 11/27/16 03:18 PM (7 years, 2 months ago)

Quote:

spacechildo said:

I mean, you have done this yourself and seen bacteria disappear once put to grains right? since you've already made the thread and posted the howto? or is this just BS THAT YOU MADE UP WITH ZERO PROOF




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Re: How to make antibacterial grains [Re: ichugwindex]
    #23872445 - 11/27/16 03:28 PM (7 years, 2 months ago)

http://www.nature.com/articles/srep19710

Is some more detailed evidence supporting ferather. I am still a little skeptical of the antibiotic properties lasting through the PC but green tea would be the way to go.


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Re: How to make antibacterial grains [Re: ichugwindex]
    #23872449 - 11/27/16 03:29 PM (7 years, 2 months ago)

I'm goin to test this theory in a week or so...I will post results of tea soaked grains-vs-water soaked grains...because I am curious myself


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Re: How to make antibacterial grains [Re: Leftfield420]
    #23872490 - 11/27/16 03:37 PM (7 years, 2 months ago)

I've just added spent tea bags cut open in the past to grains as a nitrogen boost. Doesn't hurt anything.


If you want to test the theory make his tea agar and a controlled agar. Get two sterilized qtips. Stick them in your moth and apply to the two different agars.

If his theory is correct than your tea agar will have less bacteria growth.

Green tea is more antibacterial than black. I am not sure why his teks are for black compared to green


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Re: How to make antibacterial grains [Re: vatman]
    #23872532 - 11/27/16 03:49 PM (7 years, 2 months ago)

I can do that later tonight... I have to make a couple transfers anyway and already made up plates with/without tea so, I'll sterilize some q-tips when I sterilize my x-acto knife.. And we'll see what happens


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Re: How to make antibacterial grains [Re: ichugwindex]
    #23872600 - 11/27/16 04:11 PM (7 years, 2 months ago)

Quote:

ichugwindex said:
BS THAT YOU MADE UP WITH ZERO PROOF



No I made it up after read lots of supporting information and then testing it several scenarios, did you even read the first set of posts?


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Re: How to make antibacterial grains [Re: Ferather]
    #23872606 - 11/27/16 04:12 PM (7 years, 2 months ago)

forget em.

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Re: How to make antibacterial grains [Re: Ferather]
    #23872610 - 11/27/16 04:13 PM (7 years, 2 months ago)

Here are the active ingrediants, and relative melting points: Catechins (175 °C), Tannins (200 °C), Theaflavins (240°C), Theanine (174 °C).
As you can see they can be pressure cooked with no issues and remain active after the cooking process.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflineFerather
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Posts: 6,325
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Re: How to make antibacterial grains [Re: Ferather]
    #23872637 - 11/27/16 04:22 PM (7 years, 2 months ago)

Here is a spent black tea bag (3g) enriched with grain flour (3g), and fertilizer (1g). Cooked for 2 minutes.
The bacteria grew after 24 hours, stalled, hardened and has been like that for 4 weeks.

Here is the nearly 50/50 spent tea and grain.

   

Can take images at any point.

----

Here is T-Gel enriched with the same fertilizer.

   


Here is normal T-Gel after 4 hours.




Here is the same sample a few days later.



Rescued from trich too in this case.




Here is T-Gel limed to Ph6.



No bacteria, same spawn.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
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Loc: Canada
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Re: How to make antibacterial grains [Re: Ferather]
    #23872664 - 11/27/16 04:30 PM (7 years, 2 months ago)

These are all well and good but it's not really conclusive in terms of actual application in the hobby. For instance without actual grows completed there is no way to see if there are unintended benefits or drawbacks. What kind of costs are we looking at? I make a nopour plate for about :twocents: how much do those tgel/plant ferts/teabag plates cost?

While it may seem like the mush cult regs like to just crush dreams the fact is that at the end of the day they just don't have patience for unproven junk science. Burden of proof is on the presenter. Come back with some real results and I bet you see a warmer reception.


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OfflineFerather
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Re: How to make antibacterial grains [Re: Pastywhyte]
    #23872688 - 11/27/16 04:35 PM (7 years, 2 months ago)

Ok Pastywhyte, that is a sensible decision based on your input. Good point, and will do. Back soon folks  :smile:


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflinePlantManBee
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Registered: 11/17/16
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Last seen: 1 year, 6 months
Re: How to make antibacterial grains [Re: Ferather]
    #23872758 - 11/27/16 04:59 PM (7 years, 2 months ago)

I think if the thread was named "working towards antibacterial grains" the whole kerfuffle about claims would be baseless. It's great you are doing the work and sharing, but folks were wanting to come in here and bingo, no more bacteria.

Maybe they have the right to be disappointed but are going overboard in their ire. It's not like you are getting paid for your experiments, so screw em. :evil:


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InvisiblePastywhyteMDiscord
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Re: How to make antibacterial grains [Re: PlantManBee]
    #23872762 - 11/27/16 05:01 PM (7 years, 2 months ago)

They might seem over top but when you see how many times people have come in over the years with this exact same idea and done nothing with it you can see why they lose patience quickly.


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Offlinespacechildo
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Re: How to make antibacterial grains [Re: PlantManBee]
    #23872784 - 11/27/16 05:09 PM (7 years, 2 months ago)

Quote:

PlantManBee said:
It's not like you are getting paid for your experiments, so screw em. :evil:




you gotta remember us regulars here try and help people grow mushrooms daily,
it gets real tiresome having to explain over and over why some random TEK they found on the search engine doesnt work.

So its just way easier to speak up right in the TEK and tell everyone right away what's wrong with it or how it really should be done.
And that way maybe there wont be so many new TEKs that just dont hold merit.


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OfflinePlantManBee
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Re: How to make antibacterial grains [Re: Pastywhyte]
    #23872805 - 11/27/16 05:16 PM (7 years, 2 months ago)

Quote:

Pastywhyte said:
They might seem over top but when you see how many times people have come in over the years with this exact same idea and done nothing with it you can see why they lose patience quickly.




I get it. However, welcome to getting older. There are always new people with the same questions. So, you might as well get used to it. they ALWAYS regenerate.


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OfflinecronicrFacebook
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Re: How to make antibacterial grains [Re: Ferather] * 3
    #23872812 - 11/27/16 05:16 PM (7 years, 2 months ago)

This thread has been closed.

Reason:
Ill unlock it as updates come but you guys are taking it to far with triple posts and useless bumps.


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