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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: How to make antibacterial grains [Re: Kenetic]
#23865920 - 11/25/16 12:29 PM (7 years, 2 months ago) |
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Quote:
kenetic said: I'd love to see some more info on this. No matter what I do I always seem to have a little bacteria in my spawn. I can't see it but it makes my jars smell a little musty
PC about 60g of rice, inoculate with spores, transfer to T-Agar or T-Gel balanced to Ph 6. Then isolate a clean section about 28mm away from the spawn sample.
Pass to tea infused grains or normal grains as spawn.
Mycolorado's actives testing here.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: How to make antibacterial grains [Re: Ferather]
#23866114 - 11/25/16 01:57 PM (7 years, 2 months ago) |
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Tyrannical Justice
Stranger



Registered: 09/02/16
Posts: 30
Last seen: 9 months, 19 days
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Re: How to make antibacterial grains [Re: Marty Mycfly]
#23866182 - 11/25/16 02:25 PM (7 years, 2 months ago) |
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Im definitely having a go at this one. I can never get my grains just right so this could be the key to me succeeding.
-------------------- Justice without force is powerless; force without justice is tyrannical.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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what's wrong with low ph for cube myc? only problem with low pH is that lower fungi loves it, but those cant handle higher pH, thats why we lime casing layers.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: How to make antibacterial grains [Re: spacechildo] 1
#23867072 - 11/25/16 07:18 PM (7 years, 2 months ago) |
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Im with kenetic i have bacteria issues as well this is interesting
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,363
Loc: The Inexpressible...
Last seen: 1 hour, 21 minutes
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Re: How to make antibacterial grains [Re: Boogieman47]
#23867483 - 11/25/16 10:18 PM (7 years, 2 months ago) |
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interesting 
So this method u cannot innocate with spores? Has to be a live culture to the anti-bact grains?
-------------------- "What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: How to make antibacterial grains [Re: LogicaL Chaos]
#23867655 - 11/25/16 11:47 PM (7 years, 2 months ago) |
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Sounds just like the way gentamicin sulfate kills fungal spores, including cubensis spores. Strictly speaking they CAN germinate on antibacterial agar, but it's much less likely than standard agar.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: How to make antibacterial grains [Re: LogicaL Chaos]
#23867891 - 11/26/16 05:03 AM (7 years, 2 months ago) |
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Correct
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: How to make antibacterial grains [Re: r.lutece]
#23868003 - 11/26/16 07:15 AM (7 years, 2 months ago) |
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Quote:
spacechildo said: what's wrong with low ph for cube myc? only problem with low pH is that lower fungi loves it, but those cant handle higher pH, thats why we lime casing layers.
The lower Ph T-Gel test I did, increased bacterial activity for 2 days. Ph 6 did the opposite, no activity.
Quote:
r.lutece said: Sounds just like the way gentamicin sulfate kills fungal spores, including cubensis spores. Strictly speaking they CAN germinate on antibacterial agar, but it's much less likely than standard agar.
Agreed. You can germinate mold on almost any "open air safe" grow, its just very low in chance.
Mold germinates very well on the remaining tea waste leaves, solubles removed.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: How to make antibacterial grains [Re: MudaFuka]
#23868008 - 11/26/16 07:19 AM (7 years, 2 months ago) |
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Quote:
MudaFuka said: I use tough actin Tinactin.
John Mudafukin Madden
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: How to make antibacterial grains [Re: Inocuole]
#23868150 - 11/26/16 08:23 AM (7 years, 2 months ago) |
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Here we have accidental germination of Golden oyster spores after 2.8 days. Paper, enriched with tea leaves and fertilizer, 88% water content.

Very strong, very radial, uniform growth.
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PlantManBee
undifferentiated



Registered: 11/17/16
Posts: 360
Last seen: 1 year, 6 months
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Re: How to make antibacterial grains [Re: Ferather]
#23868177 - 11/26/16 08:34 AM (7 years, 2 months ago) |
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kinda sorta related: I am doing side by side P. mexicana Jalisco truffles, one ysed a 50:50 coffee:water ratio and one 50:50 tea:water. The coffee is laying waste to the tea, though the tea is still colonizing.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: How to make antibacterial grains [Re: PlantManBee]
#23868197 - 11/26/16 08:40 AM (7 years, 2 months ago) |
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Lots of starch in coffee, its kinda like roasted rye berry's. No starch in tea, not so comparable. Still I am interested on how well that coffee does given it has been highly roasted.
Which is good for being clean to start with, bad for nutrition.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: How to make antibacterial grains [Re: Ferather]
#23868231 - 11/26/16 08:49 AM (7 years, 2 months ago) |
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Quote:
Ferather said: Lots of starch in coffee, its kinda like roasted rye berry's. No starch in tea, not so comparable. Still I am interested on how well that coffee does given it has been highly roasted.
Which is good for being clean to start with, bad for nutrition.
Bad for nutrition? Coffee is a nitrogen powerhouse and molds fast as all fuck left liquid or grounds
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: How to make antibacterial grains [Re: bodhisatta]
#23868245 - 11/26/16 08:54 AM (7 years, 2 months ago) |
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I don't disagree with it working, because it is widely used. Still what happens if you roast rye until black?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: How to make antibacterial grains [Re: Ferather]
#23868279 - 11/26/16 09:06 AM (7 years, 2 months ago) |
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Tastes like shit. Still plenty of nutrition. It doesn't evaporate off. Cubensis may not prefer if blackened but most other things couldn't give a shit. Macronutrient may be broken down sure but that doesn't matter much to most microorganisms. PH may change too but also not a big game changing thing with fungi
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PlantManBee
undifferentiated



Registered: 11/17/16
Posts: 360
Last seen: 1 year, 6 months
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Re: How to make antibacterial grains [Re: Ferather]
#23868314 - 11/26/16 09:20 AM (7 years, 2 months ago) |
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Mmmmm...i am dubious as to high starch in coffee. Comparatively sure. But it still has VERY few calories, of which a fairly high percentage is protein .
EDIT: 2 calories for an 8 oz cup. So starch and sugars.
Edited by PlantManBee (11/26/16 09:33 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: How to make antibacterial grains [Re: PlantManBee]
#23868336 - 11/26/16 09:26 AM (7 years, 2 months ago) |
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Fair enough bodhisatta, as long as my point was recognized correctly. No argument here on usefulness.
@PlantManBee, There is 28.5g of starch and 14.6g of protein per 100g dry roasted coffee.
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PlantManBee
undifferentiated



Registered: 11/17/16
Posts: 360
Last seen: 1 year, 6 months
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Re: How to make antibacterial grains [Re: Ferather]
#23868363 - 11/26/16 09:34 AM (7 years, 2 months ago) |
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We are using brewed coffee, correct? It's the water extracted values that are going to apply iiuc
Edited by PlantManBee (11/26/16 09:38 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: How to make antibacterial grains [Re: PlantManBee]
#23868370 - 11/26/16 09:36 AM (7 years, 2 months ago) |
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No offence but you are comparing brewed tea to brewed coffee, so you still have starch in the coffee as a difference. The other thing is are you using the leaves alongside, because coffee is fully soluble in comparison.
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