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OfflineShadow of magus
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Mycelium growth and burnt rye grains
    #23862696 - 11/24/16 06:41 AM (7 years, 3 months ago)

Hello Shroomery,

A few weeks ago I started running two different Teks; PF and Grain spawn and on the inoculation method I noticed some of the grain was dry or burnt. I realize why it happens and will fix it on the next run but I decided to inoculate anyway and I wanted to ask if the mycelium has a chance to colonize over the dried out part. I noticed the growth is sort of growing around it but I've also seen mycelium grow on empty space in the Jar so I'm keeping my fingers crossed. Here are some pics and thanks again for the help.




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Offlineenlightenment
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Re: Mycelium growth and burnt rye grains [Re: Shadow of magus]
    #23862700 - 11/24/16 06:46 AM (7 years, 3 months ago)

Besides the burned parts (that look like mold somehow), you also have a bacterial contamination in your jar.
How did the grains get burned?

I would start over.

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OfflineShadow of magus
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Re: Mycelium growth and burnt rye grains [Re: enlightenment]
    #23862732 - 11/24/16 07:05 AM (7 years, 3 months ago)

I'm sorry where is the bacterial growth? I didn't see any Forrest green mold and the burnt rye doesn't have mold it's just the lighting. I believe They burnt out because I didn't put the plate on the bottom to create space

Edited by Shadow of magus (11/24/16 07:07 AM)

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Offlineenlightenment
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Re: Mycelium growth and burnt rye grains [Re: Shadow of magus]
    #23862764 - 11/24/16 07:19 AM (7 years, 3 months ago)

If the burnt spots looked like this right after the PC cycle it is just burnt. The Maillard reaction hinders mycelium growth.

bacteria:

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OfflineShadow of magus
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Re: Mycelium growth and burnt rye grains [Re: enlightenment]
    #23862769 - 11/24/16 07:21 AM (7 years, 3 months ago)

Oh I see, those are just uncovered grains though, at least that's what it looks like in person. This is going to sound crazy but I can actually smell when its contaminated and this actually has a hint of sweetness to it.

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OfflineWeavieWonder
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Re: Mycelium growth and burnt rye grains [Re: Shadow of magus]
    #23862910 - 11/24/16 08:18 AM (7 years, 3 months ago)

Bacteria has many smells, and a sweet smell is one of them.  Sorry guy.  You are not completely hosed.  You can still spawn to bulk and hope for the best.  Sometimes it works out, but most of the time you end up with mold.  It might be best just to cut your losses now and start over.  Proper use of PC is very important.  Your grain shouldn't be burnt.  Use a trivet for the bottom, and don't run out of water for the love of pete.

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OfflineShadow of magus
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Re: Mycelium growth and burnt rye grains [Re: WeavieWonder]
    #23863009 - 11/24/16 09:03 AM (7 years, 3 months ago)

Thank you for the advice and it will definitely be noted but I promise all of you that those are just seeds pressed against the glass. So basically it would burnt seats there is no chance for my psyllium growth?

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OfflineBazookatooth
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Re: Mycelium growth and burnt rye grains [Re: Shadow of magus]
    #23863029 - 11/24/16 09:18 AM (7 years, 3 months ago)

Look familiar bruv?


You'll see water pooling at the bottom before those grain colonize. I did have some results but nothing compared to if my tek was more improved. Greened out completely two days after. One thing I learned and especially from these motherfuckers is they only have your best interests in mind.

Edited by Bazookatooth (11/24/16 09:23 AM)

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OfflineShadow of magus
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Re: Mycelium growth and burnt rye grains [Re: Bazookatooth]
    #23863081 - 11/24/16 09:42 AM (7 years, 3 months ago)

That's contaminated? Boy do I have a lot I learn

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OfflineWeavieWonder
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Re: Mycelium growth and burnt rye grains [Re: Shadow of magus]
    #23863371 - 11/24/16 11:30 AM (7 years, 3 months ago)

Bacteria can be hard to spot, and is a bitch.  It's not as obvious as mold.  I can't remember who said this, but it was probably the best analogy I've heard for recognizing the signs of bacterial contamination.  Contaminated grains will have that "tits pressed up against a wet glass shower door" look to them.  Also usually look greasy and the surrounding mycelium has almost a melted ice cream look to it.  A lot of the time, metabolites will be present also.  Your third picture labeled purple 11-6 is a classic example of a bacterial infection.  Here is a picture of one of my recent jars.  Also bacterial.



I inoculated this jar with an agar wedge.  I suspect my grain is dirty AF because my cultures were clean and ran a proper PC cycle @ 15psi for 120 minutes.  Gonna run my grains through the PC twice next time.

Edited by WeavieWonder (11/24/16 11:36 AM)

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Offlineenlightenment
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Re: Mycelium growth and burnt rye grains [Re: WeavieWonder]
    #23863389 - 11/24/16 11:38 AM (7 years, 3 months ago)

Good tit analogy. :laugh:

BTW:
Keep in mind that bacteria could get into jar when you inoculated it. I had 1 bacterial jar out of 10 that had the same PC cycle. Even in front of a flow hood it is possible to get contaminations. Everyone gets contaminations sometimes.

Two 120 min pc cycles is a waste of energy IMO.
Contaminations are very rare and I PC once for ~90 minutes.

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OfflineWeavieWonder
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Re: Mycelium growth and burnt rye grains [Re: enlightenment]
    #23863504 - 11/24/16 12:22 PM (7 years, 3 months ago)

Quote:

enlightenment said:


Two 120 min pc cycles is a waste of energy IMO.
Contaminations are very rare and I PC once for ~90 minutes.





That was one of 14 jars.  All of them bacterial.  I'm in the process of ruling the grain out (whole oats for race horses) as a source of contamination.  Just Ran two PC cycles on my new batch this time around just to make sure.  All grain is dirty.  Some more than others.  I've been burned by bacteria enough times, investing a couple extra hours to sterilization seems reasonable compared to the potential of loosing weeks worth of work.  Still on the fence about it.  Some would say to find a new grain supplier, but I could be trading one shit grain for another.  What do you use for grains?

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Offlineglowdiver
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Re: Mycelium growth and burnt rye grains [Re: WeavieWonder]
    #23863673 - 11/24/16 01:19 PM (7 years, 3 months ago)

I recently switched from corn to rye.  I was losing lots of jars to bacteria and struggled to come up with a good method for getting the moisture right.  After looking at what you are calling bacterial, I'd say most of my "good" jars were bacterial as well.  I've managed to get some shrooms out of them but most of my flushes were pretty poor.  We'll see how the rye fares.  I appreciate you guys sharing your knowledge.

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OfflineShadow of magus
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Re: Mycelium growth and burnt rye grains [Re: glowdiver]
    #23863739 - 11/24/16 01:55 PM (7 years, 3 months ago)

Do any of you think this can be spawned into a fruiting tray with Pete moss and dung?

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Offlineglowdiver
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Re: Mycelium growth and burnt rye grains [Re: Shadow of magus]
    #23863750 - 11/24/16 02:00 PM (7 years, 3 months ago)

Go for it. What do you have to lose?

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OfflineBazookatooth
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Re: Mycelium growth and burnt rye grains [Re: glowdiver]
    #23870871 - 11/26/16 11:52 PM (7 years, 3 months ago)

:whathesaid:

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