Home | Community | Message Board

Kratom Eye
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineTripBalls
Rocketfart
 User Gallery


Registered: 01/14/14
Posts: 170
Loc: harambe Flag
Last seen: 5 days, 10 hours
Why doesn't agar like me? I mean really, what the hell..
    #23862412 - 11/24/16 01:30 AM (7 years, 3 months ago)

Lately I've been having a tough one with my pasty plates.
I literally am getting NO visible growth whatsoever..
I have done a few transfers successfully but lately, if I get any recovery at all, the growth is VERY light and almost undetectable around the wedge..
Sometimes I'll get what looks to be a few drops amount of water that run around the agar, which I believe is from condensation.. I've been taking action to reducing condensation, but after dropping some spores on a few plates that seemed relatively dry, I still have no growth after over a week.

At this point, I'm convinced I have a clear bacterial infestation among me,
Or I'm killing the spores/wedges by not allowing the needle/blade to cool enough (which I doubt)
Maybe the incubation/germination environment isn't warm enough? (74'f)


I have no idea why the transfers didn't recover..
I PC my PDA pastywhyte plates for 30 mins, flame sterilizing the blade/needle in between plates..
Hell, my syringes are from a very reputable sponsor I've done business with for years!

What could be going wrong here?
These were supposed to be easier!

I tried taking some pictures but they really don't do justice..

Any advice is greatly appreciated. I really need some guidance!


--------------------
I Followed Frank's Footsteps This guy is a hero.
Bod’s system saved my life as well.. if these guys can’t get you where you need to be, you’re in the wrong hobby.

Rate me cause I'm a nice guy? :hi:

Not all who wander are lost, but I sure as fuck am
:mushroom2:

Extras: Filter Print Post Top
OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 4 months
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: TripBalls]
    #23862464 - 11/24/16 02:28 AM (7 years, 3 months ago)

If you're using spore syringe as inoculant, that happens with syringe to agar, sometimes they don't germinate.  Spore print to agar using an inoculation loop is more convenient.  But since you're also having problems with your transfers of already germinated mycelium, it seems like that is not the problem.  Maybe it's your recipe?  Qhat agar recipe do you use?

To get rid of condensation you can just wait for a few days before inoculating, agar absorbs the water after a while.


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.

Extras: Filter Print Post Top
Offlineenlightenment
alchemist
 User Gallery

Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: Tira]
    #23862606 - 11/24/16 05:31 AM (7 years, 3 months ago)

Quote:

Or I'm killing the spores/wedges by not allowing the needle/blade to cool enough (which I doubt)




Flame the needle, shake the syringe, flame it again. Push some drops of spore solution out of the syringe to cool the needle. Then use one drop of spore solution to inoculate the pasty plates.

Quote:

Maybe the incubation/germination environment isn't warm enough? (74'f)




It is a good temperature.

Cool the scalpel by cutting the agar of the dish you want transfer to.

Quote:

If you're using spore syringe as inoculant, that happens with syringe to agar, sometimes they don't germinate.  Spore print to agar using an inoculation loop is more convenient.


 

I experienced the opposite. Especially with old spore prints. I had to rehydrate them in spore syringes to get germination on agar.

Can you post your agar recipe?

Extras: Filter Print Post Top
InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: enlightenment]
    #23862634 - 11/24/16 06:04 AM (7 years, 3 months ago)

I'm going to assume he's using pasty recipe

1 tsp / 2.5g agar agar powder
2 tsp potato flakes (~8g)
1/2 cup water (125 ml)
1 drop honey


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself

Extras: Filter Print Post Top
Offlineenlightenment
alchemist
 User Gallery

Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: ComebackKid]
    #23862651 - 11/24/16 06:16 AM (7 years, 3 months ago)

//edit

wrong thread

Edited by enlightenment (11/24/16 06:17 AM)

Extras: Filter Print Post Top
OfflineWeavieWonder
Time Travel Sucks
Male User Gallery


Registered: 08/18/16
Posts: 1,455
Loc: USA Flag
Last seen: 2 months, 4 days
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: enlightenment]
    #23862962 - 11/24/16 08:41 AM (7 years, 3 months ago)

From research on agar work so far, I've found that germinating spores from syringe on agar can be tricky.  I've read people will inoculate a small amount of grain first, wait for it colonize a bit, then transfer the grain to agar to clean it up.  Anti bacterial agar might be useful too.  I haven't tried using a syringe on agar yet, but will in the future, as PE from vendors are only available in a syringe.  Btw, syringes are usually contaminated, I don't care what vendor you get them from.  There has to be a better solution than inoculating a shit load of plates in hopes that one will give you something to transfer over.  Thoughts anyone?

Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 3 months
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: WeavieWonder]
    #23863099 - 11/24/16 09:50 AM (7 years, 3 months ago)

I don't want you to waste money, however as WeavieWonder mentioned, you could germinate on grain then clean it.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: WeavieWonder]
    #23863119 - 11/24/16 09:57 AM (7 years, 3 months ago)

I noc a few plates at a time (3-5).  IME, some have no germination, some are loaded with bacteria and mycelium and some appear very clean.  I don't think you need to noc a shitload of plates, but you'll have more options if you at least do a few at a time.

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 4 months
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: Mycolorado]
    #23863213 - 11/24/16 10:32 AM (7 years, 3 months ago)

A week isn't that long. Ime spores germinate slower on agar than grain, I'd wait at least a month before calling it bunk. Myclium transfers should recover and show growth within less than a week though, if not you probably have some bacteria going on.

Extras: Filter Print Post Top
OfflineTripBalls
Rocketfart
 User Gallery


Registered: 01/14/14
Posts: 170
Loc: harambe Flag
Last seen: 5 days, 10 hours
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: Ferather]
    #23876711 - 11/28/16 10:26 PM (7 years, 3 months ago)

Quote:

WeavieWonder said:
From research on agar work so far, I've found that germinating spores from syringe on agar can be tricky.  I've read people will inoculate a small amount of grain first, wait for it colonize a bit, then transfer the grain to agar to clean it up.  Anti bacterial agar might be useful too.  I haven't tried using a syringe on agar yet, but will in the future, as PE from vendors are only available in a syringe.  Btw, syringes are usually contaminated, I don't care what vendor you get them from.  There has to be a better solution than inoculating a shit load of plates in hopes that one will give you something to transfer over.  Thoughts anyone?




I have 2 jars fully colonized and consolidating.. I can't believe i never thought to do a G2P isolate.
Will definitely drop a few plates.. Let's just hope I can isolate from there with no sweat!
Surely I could transfer what grains I don't use on the plates to other jars...

I hate contam vectors.. I'm very squeamish to new stuff for this reason.

Quote:

krypto2000 said:
A week isn't that long. Ime spores germinate slower on agar than grain, I'd wait at least a month before calling it bunk. Myclium transfers should recover and show growth within less than a week though, if not you probably have some bacteria going on.




Jesus! Really? I'm going for speed, this time around..
I heard about slurries and got into LC forums a bit more..
I can't/won't waste the time like I used to with multispore cultivation.
I thought agar was supposed to be the faster route!
I'll keep a few around, and hope for the best!


Quote:

Ferather said:
I don't want you to waste money, however as WeavieWonder mentioned, you could germinate on grain then clean it.




I'm almost not as worried about starting a culture now as I am isolating it!
The grain to plate transfer was an idea I hadn't even thought of yet, and I'm sure it will help tons..
But then once I get a culture, how do I know I'm not going to end up with a bunch of stalled-out wedges?

I'm still clueless as to why my transfers didn't recover? Seriously, it's like I have a completely invisible contam. Never had that problem before!


Thanks a bunch Shroomery!
You guys are great!


--------------------
I Followed Frank's Footsteps This guy is a hero.
Bod’s system saved my life as well.. if these guys can’t get you where you need to be, you’re in the wrong hobby.

Rate me cause I'm a nice guy? :hi:

Not all who wander are lost, but I sure as fuck am
:mushroom2:

Extras: Filter Print Post Top
InvisibleMoabfighter
Tam Fighter
 User Gallery

Registered: 12/13/15
Posts: 2,710
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: TripBalls]
    #23876739 - 11/28/16 10:39 PM (7 years, 3 months ago)

I use 2.0 g agar, half cup water, one teaspoon of potato flakes and one drop of Karo corn syrup.

Lest nutes give me nice rhizomorphic growth


--------------------
KSSS And PE WBS.
 

Extras: Filter Print Post Top
Offlineafflictis
ModestMycologist
Registered: 03/09/12
Posts: 334
Last seen: 5 years, 10 months
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: Moabfighter]
    #23876903 - 11/28/16 11:57 PM (7 years, 3 months ago)

Quote:

Moabfighter said:
I use 2.0 g agar, half cup water, one teaspoon of potato flakes and one drop of Karo corn syrup.

Lest nutes give me nice rhizomorphic growth


how long do you pc for

Extras: Filter Print Post Top
Offlineenlightenment
alchemist
 User Gallery

Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: afflictis]
    #23877071 - 11/29/16 02:06 AM (7 years, 3 months ago)

I usually use MEA and PC it for ~20 minutes and let it cool slowly.
20 minutes is also a good time for PDA IMO.
Don't PC it too long to prevent Maillard reaction.

Extras: Filter Print Post Top
InvisibleMoabfighter
Tam Fighter
 User Gallery

Registered: 12/13/15
Posts: 2,710
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: enlightenment]
    #23877740 - 11/29/16 09:58 AM (7 years, 3 months ago)

I PCd last night 11:20 to 12am.

So 40 mins.

My recipe really makes nice vigorous growth. Try it if you're having issues.


--------------------
KSSS And PE WBS.
 

Extras: Filter Print Post Top
OfflineSpore Ninja
PsychoMycoPhile
 User Gallery


Registered: 10/25/16
Posts: 161
Last seen: 5 years, 7 months
Re: Why doesn't agar like me? I mean really, what the hell.. [Re: TripBalls]
    #23878201 - 11/29/16 12:48 PM (7 years, 3 months ago)

Quote:

TripBalls said:


I'm still clueless as to why my transfers didn't recover? Seriously, it's like I have a completely invisible contam. Never had that problem before!


Thanks a bunch Shroomery!
You guys are great!




Is your PDA homemade?

Do you always use the same agar recipe?

I did some PDA recently and accidentally made it double strength. I should have brought the volume back up to 1000ML after boiling the potatoes, but since I was only making 500ML, I fubar'd it and only brought it back to 500ML. (Lesson learned, now i only use 100 G of potatoes and bring it back to 500ML)
I cloned onto the plates and have had almost zero growth. It was puzzling for a few days before I realized I screwed up the agar.

If the agar is the same in all your failed attempts- I'd look at changing up the agar...do something completely different - dog food agar is cheap and easy and has always worked well for me.


--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
Red Phone / Signal offer free encrypted phone calls and texting between users.

All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names.
Stay safe out there!

The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654

Edited by Spore Ninja (12/20/16 01:06 PM)

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules


Similar ThreadsPosterViewsRepliesLast post
* Re: mold in agar plate Anonymous 12,306 3 12/04/99 12:35 AM
by Anonymous
* Fruit on Agar?? Yarry 5,090 17 01/22/04 09:44 PM
by Anonymous
* golden teacher agar asking fugu 3,069 6 01/31/03 12:59 PM
by debianlinux
* Agar to Rice flour substrates foxxybrown 2,143 3 09/01/03 05:00 AM
by eggy
* agar/peroxide conc. iudexk 1,329 1 08/27/01 01:17 PM
by jonnyshaggs420
* Why grow on agar? stcore 2,017 1 07/29/02 05:03 PM
by bluhoney
* mycelium on grain transfer to agar, look ok? 2Dot 277 2 03/04/18 02:05 PM
by 2Dot
* Does these look ok to do transfers with? baddagger 478 15 03/05/18 11:36 PM
by LemurLemur

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
647 topic views. 23 members, 174 guests and 41 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.007 seconds on 12 queries.