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ColonelAngus
Stranger



Registered: 04/11/16
Posts: 124
Last seen: 5 years, 11 months
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1st time try= Mono-culture from Clone, now what ? [PICS]
#23862511 - 11/24/16 03:27 AM (7 years, 2 months ago) |
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Still a noob, but learned a lot and had some good grows over the summer. I was saving some purchased agar plates for when I felt confident to attempt some cloning.
I took some tissue from a nice looking specimen a while back, and grew it out and transferred best looking sector just twice, and ended up with many plates growing evenly in a circle. I know it's not definitively a mono-culture, but with 8 plates now all growing without sectoring, I'm guessing I may have a mono.
Now I need to test for growth characteristics and potency, but I'm debating on my best course of action.
I took one plate and noc'd 12 small 1/2 pint jars.
I see nice growth now after 3 days, and a few thin rhizomes. Since I've already noc'd the small jars, I'm wondering about the odds that this 'maybe-mono' could either totally suck, and/or worse - not grow fruits at all.
I'd prefer to G2G all the 12 small jars into larger ones, if I'm likely to get some kind of grow. But if it's really a total crap-shoot as to what I may end up with, I'm thinking maybe I take a couple of small jars and grow some kind of micro-mini-dub-tub as quickly as possible to test for potency and other traits.
Any suggestions?
Here's my plate and jars so far:
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
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Re: 1st time try= Mono-culture from Clone, now what ? [PICS] [Re: ColonelAngus]
#23862525 - 11/24/16 03:43 AM (7 years, 2 months ago) |
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If you took a tissue from a fruitbody chances are very high that the culture you isolated is going to produce fruits (I never had a clone that did not produces fruits).
I like to test my isolates or clones in trays before I waste too much resources for a crap genetic.
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ColonelAngus
Stranger



Registered: 04/11/16
Posts: 124
Last seen: 5 years, 11 months
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Re: 1st time try= Mono-culture from Clone, now what ? [PICS] [Re: enlightenment]
#23862918 - 11/24/16 08:23 AM (7 years, 2 months ago) |
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Quote:
enlightenment said: If you took a tissue from a fruitbody chances are very high that the culture you isolated is going to produce fruits (I never had a clone that did not produces fruits).
I like to test my isolates or clones in trays before I waste too much resources for a crap genetic.
Ok, thank you for enlightening me. That's encouraging about the growth. I have these 12 mini jars which I could either G2G and expand, or take a few once colonized and spawn to a very small mono or micro dub-tub just to get enough growing to observe and sample.
I guess I'll try the latter so as not to disappoint myself with a shelf full of potentially worthless quarts.
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
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Re: 1st time try= Mono-culture from Clone, now what ? [PICS] [Re: ColonelAngus]
#23863323 - 11/24/16 11:11 AM (7 years, 2 months ago) |
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Was your cloned fruit a mono culture or did you remove strains from it? Most fruits have multiple strains comprising them. If you remove some it will probably still fruit but they usually do not perform as well on their own as they do together.
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ColonelAngus
Stranger



Registered: 04/11/16
Posts: 124
Last seen: 5 years, 11 months
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Re: 1st time try= Mono-culture from Clone, now what ? [PICS] [Re: krypto2000]
#23864513 - 11/24/16 07:46 PM (7 years, 2 months ago) |
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Quote:
krypto2000 said: Was your cloned fruit a mono culture or did you remove strains from it? Most fruits have multiple strains comprising them. If you remove some it will probably still fruit but they usually do not perform as well on their own as they do together.
I took some tissue from a multi-spore grow. But regardless, if a mono culture wouldn't ever perform well on its own, then why would anyone try to isolate a mono?
I understand that you cannot adequately judge the character of an isolated strain based on what's growing on a plate. I simply grew from the tissue of the largest and fastest-growing fruit during my 1st flush, then took a small sector from the fastest growing and most rhizomal looking sector of my 1st plate. That next plate grew in a perfect circle with even characteristics, and then I grew 6 new plates from that, which all grew identically.
Should be an interesting experiment. I'll report back here what happens. Hopefully I'll have some beginner's luck, and if so I've got a bunch of slants ready for long-term storage!
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