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OfflineMushiesInValhalla
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Oh god.. FUBAR
    #23861083 - 11/23/16 03:44 PM (7 years, 2 months ago)

Ok so I may have an issue.. Two days ago I nocced 13 jars. I used a wbs tek I found and followed it to the T except I unfortunately do not have a PC so I pasteurized for an hour. The moisture was good from what I could tell because when I grabbed a handful of the wbs only 5-10 grains stuck to my hand. Either way, after the last grab test I let them sit and cool for an additional 20 minutes before I boiled them, only letting the water come up about halfway on each jar.

Each jar was also covered completely with a double layer of foil which I made sure to crinkle around the sides so additional moisture would not permeate. After the hour or so was up, I made the mistake of removing them from the water to cool overnight in the air rather than gradually along with the water which is why I believe I've run into my excessive moisture problem. There is standing water at the bottom of the jars and although it isn't much, the fact that I nocced them the following day, adding more water to it, I fear I may have a serious problem. The grains soaked up a lot of the residual moisture from the cooking process but there is still a little bit of standing water.

I was very meticulous in the preparation of all materials being used and sterilized the hell out of my work area and everything around it. I used Lysol, rubbing alcohol, a face mask, rubber gloves, etc. they are currently incubating at between 65-70 degrees F so I know that any mycelium growth will take quite a bit longer to notice.

Additionally, 5 of the jars I used were pre-made cakes that my buddy ordered online and were supposedly already sterile yada yada. But just to be safe I also pasteurized those jars as well. The spawn material inside seemed super dry so I added maybe 1-2 tbsp of distilled water and then nocced them.

My questions are thus: 1- Are my wbs jars fucked or is there still hope as long as I don't see any contam?

And 2- Did I fuck up the pre made cakes when I added water or is there still hope for them as well?


UPDATE: Eureka!!! Checked the jars today and 4/13 of them are showing positive signs of colonization! I noticed a few very tiny bright white blotches at several noc points. Unfortunately 1 out of the remaining 8 jars MAY have contamination. I separated that jar from the rest to be monitored individually. It's still too early to be 100% about the 4 I believe to be colonized so I'm not getting my hopes up just yet. However if I am correct, I only hope they begin to colonize more aggressively in order to fight off any potential contams in the coming days/weeks.


--------------------
Shrooms? Nah I don't have any of those but are you sure you don't wanna buy some Trich, bro?



Edited by MushiesInValhalla (11/24/16 10:13 AM)


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OfflinecronicrFacebook
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Re: Oh god.. FUBAR [Re: MushiesInValhalla]
    #23861089 - 11/23/16 03:46 PM (7 years, 2 months ago)

You don't have a chance...followed the tek to a T except sterilizing lol


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineMushiesInValhalla
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Re: Oh god.. FUBAR [Re: cronicr]
    #23861095 - 11/23/16 03:49 PM (7 years, 2 months ago)

Quote:

cronicr said:
You don't have a chance...followed the tek to a T except sterilizing lol




Well shit.. I know the obvious response to my next question will simply be "Get a PC" lol but I have to ask, how long should they be pasteurized if an hour won't do?


--------------------
Shrooms? Nah I don't have any of those but are you sure you don't wanna buy some Trich, bro?



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OfflinecronicrFacebook
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Re: Oh god.. FUBAR [Re: MushiesInValhalla]
    #23861098 - 11/23/16 03:50 PM (7 years, 2 months ago)

They should be steamed not pasturize for 8 hours minimum


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineMushiesInValhalla
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Re: Oh god.. FUBAR [Re: cronicr]
    #23861109 - 11/23/16 03:55 PM (7 years, 2 months ago)

What about the pre-made jars? They already come "sterile" but I cooked em a bit anyway. Of the wbs is screwed is there at least hope for them?


--------------------
Shrooms? Nah I don't have any of those but are you sure you don't wanna buy some Trich, bro?



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OfflinecronicrFacebook
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Re: Oh god.. FUBAR [Re: MushiesInValhalla]
    #23861111 - 11/23/16 03:57 PM (7 years, 2 months ago)

They might colonize...they are supposed to be on the dry side though


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineMushiesInValhalla
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Re: Oh god.. FUBAR [Re: cronicr]
    #23861117 - 11/23/16 04:01 PM (7 years, 2 months ago)

Ah alright so there's a chance lol that's all I need. Thank you for taking the time to respond. Back to the drawing board I suppose. Luckily I still have a full syringe.


--------------------
Shrooms? Nah I don't have any of those but are you sure you don't wanna buy some Trich, bro?



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OfflinecronicrFacebook
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Re: Oh god.. FUBAR [Re: MushiesInValhalla]
    #23861124 - 11/23/16 04:03 PM (7 years, 2 months ago)

Well you can get by without one if your willing to put the work in

https://www.shroomery.org/forums/showflat.php/Number/19296230


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisiblemorty422
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Re: Oh god.. FUBAR [Re: cronicr]
    #23861134 - 11/23/16 04:06 PM (7 years, 2 months ago)

Quote:

cronicr said:
Well you can get by without one if your willing to put the work in

https://www.shroomery.org/forums/showflat.php/Number/19296230




I'll never get tired of that thread of yours cron... haha


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OfflineMushiesInValhalla
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Re: Oh god.. FUBAR [Re: morty422]
    #23861176 - 11/23/16 04:19 PM (7 years, 2 months ago)

Depending on what happens in the next couple of weeks, I may try my hand had brf cakes.. Generally speaking I know that there's little room for error with anything you use as a spawn but wbs seems to be especially unforgiving..


--------------------
Shrooms? Nah I don't have any of those but are you sure you don't wanna buy some Trich, bro?



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Offlinetump
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Re: Oh god.. FUBAR [Re: MushiesInValhalla]
    #23861540 - 11/23/16 06:22 PM (7 years, 2 months ago)

You can cook it 2 hours for 3 days in a row with some success. Pc is easier.


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Offlinespacechildo
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Re: Oh god.. FUBAR [Re: MushiesInValhalla]
    #23861605 - 11/23/16 06:45 PM (7 years, 2 months ago)

Quote:

MushiesInValhalla said:
Depending on what happens in the next couple of weeks, I may try my hand had brf cakes.. Generally speaking I know that there's little room for error with anything you use as a spawn but wbs seems to be especially unforgiving..




When Loke wanted to trip off some homegrown he didnt have a PC either... so he went for the pf-tek www.mushroomvideos.com :thumbup:


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OfflineMushiesInValhalla
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Re: Oh god.. FUBAR [Re: spacechildo]
    #23861709 - 11/23/16 07:18 PM (7 years, 2 months ago)

When Loke wanted to trip off some homegrown he didnt have a PC either... so he went for the pf-tek www.mushroomvideos.com :thumbup:




Yeah I'm probably going to go with that to start off. I was initially going to try to hit the ground running with this wbs bulk tek but apparently this is one of those things that requires a "Crawl-Walk-Run" approach lol fml.


--------------------
Shrooms? Nah I don't have any of those but are you sure you don't wanna buy some Trich, bro?



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Offlinespacechildo
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Re: Oh god.. FUBAR [Re: MushiesInValhalla]
    #23861741 - 11/23/16 07:30 PM (7 years, 2 months ago)

nah, not really, but it requires a PC :super:

unless you wanna do it like cron did, but he's pure dedication!


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OfflineMushiesInValhalla
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Re: Oh god.. FUBAR [Re: spacechildo]
    #23861764 - 11/23/16 07:40 PM (7 years, 2 months ago)

So basically using wbs is pointless unless you have a PC? Boiling for an hour won't do anything positive as far as killing unwanted contams? Welp.. There goes $45.00. Lesson learned.


--------------------
Shrooms? Nah I don't have any of those but are you sure you don't wanna buy some Trich, bro?



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OfflinecronicrFacebook
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Re: Oh god.. FUBAR [Re: MushiesInValhalla]
    #23861793 - 11/23/16 07:52 PM (7 years, 2 months ago)

Even cakes need 90 minutes


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineMushiesInValhalla
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Re: Oh god.. FUBAR [Re: cronicr]
    #23861808 - 11/23/16 08:03 PM (7 years, 2 months ago)

Quote:

cronicr said:
Even cakes need 90 minutes




Yeah I guess I'll just let what I have nocced run for another week or so and check for contams.
In the meantime I have a friend that's letting me borrow his PC and I'm gonna do a few brf cakes with the last 13cc's I have and hope for the best.


--------------------
Shrooms? Nah I don't have any of those but are you sure you don't wanna buy some Trich, bro?



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Offlinespacechildo
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Re: Oh god.. FUBAR [Re: MushiesInValhalla]
    #23861818 - 11/23/16 08:08 PM (7 years, 2 months ago)

brf cakes = no PC needed.
wbs/grains etc = PC needed.


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Offlinetump
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Re: Oh god.. FUBAR [Re: spacechildo]
    #23861869 - 11/23/16 08:36 PM (7 years, 2 months ago)

What cost you 45 bucks in bird seed.


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OfflineMushiesInValhalla
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Re: Oh god.. FUBAR [Re: tump]
    #23861886 - 11/23/16 08:42 PM (7 years, 2 months ago)

Quote:

tump said:
What cost you 45 bucks in bird seed.




No the syringes I used for inoculation.


--------------------
Shrooms? Nah I don't have any of those but are you sure you don't wanna buy some Trich, bro?



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