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OfflineMortySmith
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Registered: 03/28/16
Posts: 68
Last seen: 6 years, 4 months
Agar to oats
    #23860624 - 11/23/16 01:17 PM (7 years, 2 months ago)

Inoculated my first grain jars with agar wedges! Used pasty's plates and the tiger drop 2.0 method to inoculate 2 quart jars of whole oats.

11-23



Before you comment, I gotta say, it was my first time, so of course I messed everything up.

First, I did 3 plates all of different strains, didn't label any of them. :facepalm:

Then I took 2 transfers from the best looking mystery plate, which both ended up being clean. But by the time they were all colonized and ready for inoculation, I was not ready with the grains!  :facepalm:
I decided to put them in the fridge as I read that is where you store them if not using.

Finally got 2 jars prepped, first time using oats. I made them way too full though. It was hard to get the wedges shaken up in there, but now I know to use a few less oats next time per jar I guess.

Did the inoculation, tiger drop 2.0. The mycelium looked completely dead, I honestly thought maybe being in the fridge like they were killed it, like maybe I missed some key step in the fridge storage process. But today, 3 days after inoculation, I can see some white fuzzy edges around many of the wedges!

So far, so good, right?! What do you guys think?  :grin:

12-06 UPDATE:

Wow, lots of progress. I planned on updating this post before I shook, but never got around to it. Managed to snap a pic every few days at least!

11-26


11-28


11-29


12-01


12-02


12-03


12-06


So as you can see, they are nearly done. The last picture is a close-up of the area still colonizing. How do these look to you guys? Healthy?
Also, is there some sort of "consolidation" period I should wait once that area is finished?


Edited by MortySmith (12/06/16 02:31 PM)


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: Agar to oats [Re: MortySmith]
    #23861029 - 11/23/16 03:25 PM (7 years, 2 months ago)

Looks good so far :thumbup:
I filled my jars way too full on my current grow and it's a huge PITA. Just wait til you have to shake it uggghhh


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OfflineMortySmith
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Registered: 03/28/16
Posts: 68
Last seen: 6 years, 4 months
Update [Re: MortySmith]
    #23900110 - 12/06/16 02:31 PM (7 years, 1 month ago)

Updated the post with more pictures


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