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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cleaning dirty cultures with tea agar [Re: eatyualive] 1
#24500922 - 07/22/17 02:19 PM (6 years, 6 months ago) |
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here is the matias romero. i believe this is after 7 days.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: eatyualive]
#24502183 - 07/23/17 06:16 AM (6 years, 6 months ago) |
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Very thick, and I see lots of variation. 12 and 6 o'clock look fast.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24502201 - 07/23/17 06:33 AM (6 years, 6 months ago) |
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If you are interested, add some CaCO3 powder until pH 7-8, which should increase speed. The CaCO3 will also add more soluble carbon variation to the agar media.
Tea is acidic, as a bonus more of it breakdowns to CaCO3.
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Shroomway
It's me!


Registered: 07/05/17
Posts: 438
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24502223 - 07/23/17 06:59 AM (6 years, 6 months ago) |
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Would there be any disadvantage to experimenting with tea in substrates? Just to cut down the chances of bacterial contamination during colonization in jars and spawn bags. Let's say a low dose of tea in the cooking water for grains? Moisturizing manure, straw, coir, ect using a low dose of tea-water instead of plain water? It sounds interesting. Never knew tea is such an effective anti bacterial agent.
Is it known why tea is so effective against bacteria? Is it just too acidic or???
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24502225 - 07/23/17 07:01 AM (6 years, 6 months ago) |
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Yeah plan on transferring when the fam leaves
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Cleaning dirty cultures with tea agar [Re: eatyualive]
#24502245 - 07/23/17 07:21 AM (6 years, 6 months ago) |
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Quote:
Shroomway said: Would there be any disadvantage to experimenting with tea in substrates? Just to cut down the chances of bacterial contamination during colonization in jars and spawn bags. Let's say a low dose of tea in the cooking water for grains? Moisturizing manure, straw, coir, ect using a low dose of tea-water instead of plain water? It sounds interesting. Never knew tea is such an effective anti bacterial agent.
Is it known why tea is so effective against bacteria? Is it just too acidic or???
Here is a link to some natural alternatives, you can google more on tea extracts. Adding it to grain requires many steps. You will need to correct the carbon-to-nitrogen ratio, and pH, which means testing, not sure it's worth it.
I did one test, the result was the bacteria did stall, but mold (mycelium) grew everywhere. Ultimately it depends what contamination you get most often, mold or bacteria.
Quote:
eatyualive said: Yeah plan on transferring when the fam leaves
Awesome, great idea with the media variation, looks like it's doing what you want.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: Cleaning dirty cultures with tea agar [Re: Ferather] 1
#24502480 - 07/23/17 09:51 AM (6 years, 6 months ago) |
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transferred those two sectors. one to another tea plate and the other to a pda plate. didn't waste time did the transfer before the fam was gonme 30 minutes.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
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Re: Cleaning dirty cultures with tea agar [Re: eatyualive]
#24504474 - 07/24/17 07:30 AM (6 years, 6 months ago) |
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Has anyone tried my sucrose (carbon source) + soluble nutrients recipe, vs, malt extract agar? It works really well for oysters, and produced strong growth, it's mildly antibacterial.
100g > CaCO3 Water, pH 7.5 - 8.2.
0.1g > Miracle-Gro Nutrients. 0.2g > 240B Gelatin. 2g > Sucrose.
2g > Agar
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24504520 - 07/24/17 08:10 AM (6 years, 6 months ago) |
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Gelatin (peptides): Composition.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24506809 - 07/25/17 09:41 AM (6 years, 6 months ago) |
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Here are the results of the C-Gel agar, and how to use it. And the next T-Gel agar recipe. Thanks to, katbusa, Mycolorado and eatyualive for their test's and recipes.
You can also use it with malt extract, sucrose and so on.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24513689 - 07/28/17 09:51 AM (6 years, 6 months ago) |
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Here are the results of the updated T-Gel recipe, twice as strong, twice as fast. Sorry for the image quality, there is condensate on the lid currently.
Scrapings also grow out, but growth is best from the peg.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24514072 - 07/28/17 01:48 PM (6 years, 6 months ago) |
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Another comparison is, there is instant regeneration and growth on the peg, whereas recipe 1 did not regenerate.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24514084 - 07/28/17 01:50 PM (6 years, 6 months ago) |
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No starch, sugar or cellulose is being used in either recipe.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24514101 - 07/28/17 02:06 PM (6 years, 6 months ago) |
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Use the damn edit button
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: bodhisatta]
#24514117 - 07/28/17 02:19 PM (6 years, 6 months ago) |
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Lol, sorry. Will do.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24525836 - 08/02/17 08:07 PM (6 years, 5 months ago) |
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Just popped by to show you my progress so far. Ape myc on T agar day 16

Took a while for it to leap off the wedge but after it did it seemed to be growing steadily.
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: ComebackKid]
#24527647 - 08/03/17 02:28 PM (6 years, 5 months ago) |
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Was that scum formation already there when it set? Also is this a dirty culture? If that is scum, then id say the mycelium outpaced the contamination.
If it's not contaminated, then I see adaption to media.
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24546170 - 08/11/17 01:48 PM (6 years, 5 months ago) |
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So, what's the least amount of tea necessary to get benefits? Does PCing reduce the antibacterial properties? Could I just add a small pinch of lime, because I don't have a pH meter?
Also, from what I gather, tea can be mixed directly with agar, without adding nutrients from another source, is that correct? So, I could just make the tea, and add the agar powder, and voila?
I'm doing this right now. I used like 12 tea bags for maybe a gallon and a half of tea. I was just making some sweat tea for the family. It seems as though I probably didn't use enough tea, but I'll give it a go. I am now boiling the tea with some grains to add a bit of starch, and then I intend to add 50% H2O to that. Not as exact or sciency as some of you folks lol
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Bigbadwooof]
#24546717 - 08/11/17 05:47 PM (6 years, 5 months ago) |
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You can use 100% tea extract and agar for wood-loving, can't say for other mycelium. If you know your mycelium can produce laccase then try 100% tea extract.
6-8g of lime to 100g of tea extract should be close.
Yes you can pressure cook it.
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24546755 - 08/11/17 06:06 PM (6 years, 5 months ago) |
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But what concentration of tea? Normal drinking strength?
I believe I read one poster steeped 25g in a quart worth of water. It seems quite excessive to me. Then I read your post, which was I think 6g to 250ml (about the same)? What is the effective range. I assume it's preferable to stay on the lower end of that range, and I'd rather drink my tea
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
Edited by Bigbadwooof (08/11/17 06:07 PM)
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