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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24475057 - 07/11/17 11:35 AM (6 years, 6 months ago) |
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Yea I've seen that already wondering if you actually grew some mushrooms though like to prove that grain transfer worked well
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: bodhisatta]
#24475079 - 07/11/17 11:42 AM (6 years, 6 months ago) |
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You mean like Mattisfat's king oyster log? As far as TA you will need to ask him. Here is WBS, then WBS to WL-Tek.

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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24475095 - 07/11/17 11:47 AM (6 years, 6 months ago) |
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Oysters on WL tek isn't compelling they already grow on cellulose product
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: bodhisatta]
#24475123 - 07/11/17 11:55 AM (6 years, 6 months ago) |
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What do you think coco coir is composed of?
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MagikShrooms
INK13JR

Registered: 07/16/12
Posts: 76
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24475126 - 07/11/17 11:57 AM (6 years, 6 months ago) |
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Are those the centrifugal plastic vials you mentioned in your initial post?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: MagikShrooms]
#24475137 - 07/11/17 12:00 PM (6 years, 6 months ago) |
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Yes, they are 2ml centrifugal vials, which is a perfect size for 1/2 a toothpick.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24475248 - 07/11/17 12:41 PM (6 years, 6 months ago) |
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Coir isnt the food the spawn is I don't think I've seen a straight coir grow...
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MagikShrooms
INK13JR

Registered: 07/16/12
Posts: 76
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24475262 - 07/11/17 12:45 PM (6 years, 6 months ago) |
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Awesome, thank you. They look like they can be very handy. Like single use inoculation media,
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
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Re: Cleaning dirty cultures with tea agar [Re: MagikShrooms]
#24475687 - 07/11/17 03:32 PM (6 years, 6 months ago) |
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I missed the open air knock bit as well
So you boil your grains in tea or..? Bulk sub too?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Psilosopherr]
#24475727 - 07/11/17 03:48 PM (6 years, 6 months ago) |
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Quote:
bodhisatta said: Coir isnt the food the spawn is I don't think I've seen a straight coir grow...
No, that's not really what is happening, the spawn acts as the nutrient source in that scenario, in the same way as adding grain flour to verm. Surely this has been tried, so what is better, 50g grain spawn + 50g dry verm + water, or, 50g grain spawn + 50g dry coir + water.
If the verm was better, surely you would prefer this over coir, as the verm will not contaminate, unless you add things. Coco coir is considered "cold" due to lack of nutrients, composed mostly of cellulose, hemicellulose and lignin.
Quote:
Psilosopherr said: I missed the open air knock bit as well
So you boil your grains in tea or..? Bulk sub too?
??
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24475732 - 07/11/17 03:50 PM (6 years, 6 months ago) |
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If verm was cheaper than coir I probably would use all verm substrate..
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: bodhisatta]
#24475769 - 07/11/17 04:02 PM (6 years, 6 months ago) |
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So a side by side has been done, considering you could measure a small amount, say 100g. Can I have the information for this side-by-side, and relational recipe's.
Thanks.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24476195 - 07/11/17 07:02 PM (6 years, 6 months ago) |
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I have done rez effect a few times. It was nearly identical to the coir. If verm was cheaper than coir I would use it in far larger amounts. The only thing is it does best cased with something other than verm. But as far as yeild when you do case with a mix, it's pretty well the same.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cleaning dirty cultures with tea agar [Re: Pastywhyte]
#24490023 - 07/17/17 06:23 PM (6 years, 6 months ago) |
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Finally giving this a shot
Just mixed 2 grams of red rose orange pekoe into munches 1 2 3 pda recipe. Can't wait to run my cultures through it. 
Lmao my dumbass dropped a couple drops of green food colouring in there so I could differentiate between the tea agar and my regular blue agar plates. Completely forgot that tea turns fucking brown anyway 
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cleaning dirty cultures with tea agar [Re: ComebackKid]
#24490172 - 07/17/17 07:39 PM (6 years, 6 months ago) |
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.
I had to let the plates go due to life. I'll do another round of transfers on the tea agar next. Maybe LI The next batch. Reading back on this thread, I was planning on transferring at day 3 before I got busy. The growth looked good but was slow. I'm hoping I cam peak at it soon and sneak in some transfers. Doubtful though.
Edited by eatyualive (07/17/17 07:48 PM)
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,584
Loc: Canada
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Re: Cleaning dirty cultures with tea agar [Re: eatyualive]
#24491224 - 07/18/17 08:50 AM (6 years, 6 months ago) |
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Great thread. Will definitely try this out. Thanks !
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Snazz]
#24495759 - 07/20/17 06:51 AM (6 years, 6 months ago) |
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The tea extracts are very acidic, did you check the end pH?
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24499609 - 07/21/17 08:19 PM (6 years, 6 months ago) |
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No I did not. I'm assuming that is the reason the myc hasn't let off the wedge onto the plate yet? 
Unless that normal for 4 days after transfer? I know I should expect slow growth but this is getting rediculous
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: ComebackKid]
#24500333 - 07/22/17 07:38 AM (6 years, 6 months ago) |
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Here is C-Gel agar, which is another recipe I am trying, so far it works as intended, growth is quicker. However I have no idea how well the C-Gel recipe will work for cubensis at this time.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24500382 - 07/22/17 08:24 AM (6 years, 6 months ago) |
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All my plates colonized except the culture of matias Romero I was cleaning up. Its about 50%.
Edited by eatyualive (07/22/17 10:05 AM)
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