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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Ferather] 1
#24467229 - 07/08/17 09:24 AM (6 years, 6 months ago) |
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24467300 - 07/08/17 09:51 AM (6 years, 6 months ago) |
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I was just looking at this the other day Ferather. Love it!
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Cleaning dirty cultures with tea agar [Re: eatyualive]
#24467388 - 07/08/17 10:24 AM (6 years, 6 months ago) |
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All you need is metal tweezers, cheap and easy to get, easy to clean or sterilize. Transfers take seconds, the area of contact is away from the agar.
You can also replace removed pegs with new pegs.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24468063 - 07/08/17 04:30 PM (6 years, 6 months ago) |
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Ok made up some no pour plates today.
used
1 cup brewed tea from the tea maker.(6 grams tea-3 bags, 4 cups water) 1 tablespoon or close to 6 grams MEA.
after the tea brew, cooked it on medium heat and added the agar. stirred until the mix was consistent. then poured into plates.
pced for 30 minutes at 15-17 psi.


Edited by eatyualive (07/10/17 12:00 PM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: eatyualive]
#24469864 - 07/09/17 11:27 AM (6 years, 6 months ago) |
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Do you know how much tea that was? Sorry I work by weighing, an estimate is fine, thnx.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24470342 - 07/09/17 02:38 PM (6 years, 6 months ago) |
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Did I mention that I used just hot water (no pressure cook) and I assembled everything open air. If I do the same with an agar recipe with sugar or starch I need to PC, and get mold.
I still have no contamination, and I'm adding transfer pegs tomorrow.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24470998 - 07/09/17 07:11 PM (6 years, 6 months ago) |
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4 cups water was brewed with 6 grams tea.1 cup of that water was used for the plates.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Cleaning dirty cultures with tea agar [Re: eatyualive]
#24471831 - 07/10/17 06:17 AM (6 years, 6 months ago) |
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Ok, thank you.
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Lennybernadino
Amazon grower



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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24471846 - 07/10/17 06:32 AM (6 years, 6 months ago) |
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Great improv with the tooth picks, I think use this method also with pieces of straw, or stinging nettle, or whatever substrate you want to train the myc for. I suddenly think of myclial expansion as a pre-school for the myc and every bit of substrate is a teaching opportunity. I like to put bits of whatever substrates or spawn mediums in the agar that not only select out the strains but to give them a genetic memory of how to deal with these subs in the future. I theorize that you can train mycelium to deal with all sorts of conditions. By retaining a preserved culture of the original isolate I suspect you may be able to inoculate the fresh mycelium with the mycelial memory of the same strain after it has been through a lot, Thereby maintaining vigor and the learned responses to different situations. Think of it as a Grandad myc passing it's Knowledge to this grand son who has a long brilliant futre ahead of it and all of the infinite potential in the universe.
Edited by Lennybernadino (07/10/17 06:34 AM)
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Morelman
LC Master



Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree
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Re: Cleaning dirty cultures with tea agar [Re: Mycolorado]
#24471898 - 07/10/17 06:59 AM (6 years, 6 months ago) |
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EdiblesOnly
on a roller coaster

Registered: 12/27/12
Posts: 646
Loc: Mexico
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Re: Cleaning dirty cultures with tea agar [Re: Morelman]
#24471911 - 07/10/17 07:05 AM (6 years, 6 months ago) |
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This is awesome. Thanks for sharing. This should make clean up easier.
@ ferather u seriously do not pc with this method?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Morelman]
#24471913 - 07/10/17 07:06 AM (6 years, 6 months ago) |
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Thanks everyone, this thread is open so feel free to experiment, share and discuss the topic.
@EdiblesOnly, the recipe in my signature, nope no pressure cook, open air.
Edited by Ferather (07/10/17 07:21 AM)
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blueconfusion
Strangest


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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24471949 - 07/10/17 07:22 AM (6 years, 6 months ago) |
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MagikShrooms
INK13JR

Registered: 07/16/12
Posts: 76
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Re: Cleaning dirty cultures with tea agar [Re: Mycolorado]
#24472012 - 07/10/17 07:59 AM (6 years, 6 months ago) |
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Neat, thank you for bringing this to my attention. Might have to give it a whirl!
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Cleaning dirty cultures with tea agar [Re: MagikShrooms]
#24472051 - 07/10/17 08:26 AM (6 years, 6 months ago) |
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No problem. The carbon-nitrogen ratio of the T-Gel agar is about 30:1, roughly, using non sugar carbon.
List of carbon sources, remaining required nutrients from the tea and gelatin. The nutrients come in a natural and organic water soluble format.
Calcium bicarbonate: [ Ca | C | H | O3 ] 2. Proteins, varied: [ Cx | Hx | Ox | Nx ] x. Polyphenols, varied: [ C6 | H5 | O ] x.
Theophylline: C7 | H8 | N4 | O2. Theobromine: C7 | H8 | N4 | O2. Caffeine: C8 | H10 | N4 | O2. Theaflavin: C29 | H24 | O12. Tannins: C76 | H52 | O46. Catechin: C15 | H14 | O6. Carotene: C40 | H56.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24472372 - 07/10/17 11:31 AM (6 years, 6 months ago) |
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I kinda worked the recipe into the way I usually do my no pour plates. Since I had mea I just assumed 6 grams total.
Transfers are looking good today. I'll get pics up in a few days. I also wanted to see if a slight variation would work. I like mixing it up. That amount I stated above poured 12 glad rounds.
The soul intent of me using this was for an old cubie called matias Romero. First swipe looks clean but wanted to try it out. I'd also like to spread the spores on this matias Romero. Its one of my old favorites from 99-2000.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: eatyualive] 1
#24472498 - 07/10/17 12:31 PM (6 years, 6 months ago) |
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Makes sense, nutritional variation should allow it to detect-digest more things, or if you like, use more genetic blueprints.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Ferather] 1
#24472644 - 07/10/17 01:31 PM (6 years, 6 months ago) |
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Here are today's images of my T-Gel agar, no pressure cook, open air assembly, no contamination.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#24473138 - 07/10/17 04:15 PM (6 years, 6 months ago) |
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There's contamination it's just not germinating/growing. It's not like you're keeping it out, you're keeping it at bay
Who's moved off of open air tea agar back to sterilised agar? Or taken open air agar and inoculated sterilized spawn?
Is there a way to accomplish that or do you take you open air agar to open air-able grows?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: bodhisatta] 3
#24474960 - 07/11/17 10:58 AM (6 years, 6 months ago) |
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Both agar and grain transfer without infection, from open air transfers, here is the post I already provided.
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