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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 8 hours, 16 minutes
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Re: Cleaning dirty cultures with tea agar [Re: Edmunter]
#23863053 - 11/24/16 09:30 AM (7 years, 2 months ago) |
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Fuck yeah I might go get some tea now and keep it in my cabinet just to try this out when the time comes
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Edmunter]
#23863061 - 11/24/16 09:34 AM (7 years, 2 months ago) |
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Quote:
Edmunter said: Now youve gone and done it, The missus wants to know WTF I want litmus paper for........ I blamed you......
Lol, sorry. They are dirt cheap, got 160 for £1.50 free P&P. Cut them in half, you get 320.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Cleaning dirty cultures with tea agar [Re: Leftfield420]
#23863176 - 11/24/16 10:18 AM (7 years, 2 months ago) |
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Quote:
Leftfield420 said: Fuck yeah I might go get some tea now and keep it in my cabinet just to try this out when the time comes
Are you a heathen, U cant live in England unless you have tea in your cupboard
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Cleaning dirty cultures with tea agar [Re: Edmunter]
#23863437 - 11/24/16 11:52 AM (7 years, 2 months ago) |
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I like this!
Should of prepped my recent plates with some cinnamon/tea, oh well.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Teemo 6T3]
#23863935 - 11/24/16 03:36 PM (7 years, 2 months ago) |
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I have a request Mycolorado, if you have the time. Could you try a starch free version? You can transfer from one of those T-Agars you have now, Ph 6 via lime.
You shouldn't need to pressure cook. Thanks again.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#23864081 - 11/24/16 04:40 PM (7 years, 2 months ago) |
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Of course. Out of town on holiday but will put some together this weekend.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Mycolorado]
#23864091 - 11/24/16 04:44 PM (7 years, 2 months ago) |
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Top man, no rush whatsoever
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Neomorph


Registered: 12/10/14
Posts: 767
Loc: Europe
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#23864222 - 11/24/16 06:03 PM (7 years, 2 months ago) |
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Leftfield420
bong toker



Registered: 02/26/16
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Re: Cleaning dirty cultures with tea agar [Re: Edmunter]
#23864244 - 11/24/16 06:11 PM (7 years, 2 months ago) |
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Man I don't drink tea...and don't live in england..
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catnip40
xฬ็



Registered: 03/09/12
Posts: 703
Last seen: 8 days, 22 hours
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Re: Cleaning dirty cultures with tea agar [Re: Leftfield420]
#23864930 - 11/24/16 11:16 PM (7 years, 2 months ago) |
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Cleaning dirty cultures with tea agar [Re: Leftfield420]
#23865400 - 11/25/16 07:49 AM (7 years, 2 months ago) |
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Quote:
Leftfield420 said: Man I don't drink tea...and don't live in england..
Then u know how lucky u are.......
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Cleaning dirty cultures with tea agar [Re: Edmunter]
#23880725 - 11/30/16 05:48 AM (7 years, 1 month ago) |
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Ok so I took this

and made 2 sandwiches


Next minute im free(next week) I will make a Tea plate to compare. Im still not sure this plate is contaminated but its worth a try.
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Neomorph


Registered: 12/10/14
Posts: 767
Loc: Europe
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Re: Cleaning dirty cultures with tea agar [Re: Edmunter]
#23905951 - 12/08/16 07:29 AM (7 years, 1 month ago) |
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should i just boil the bags or throw the tea powder on the mix ?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Neomorph]
#23906013 - 12/08/16 08:02 AM (7 years, 1 month ago) |
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I use the bag method, you can do leaves and strain. You want the solubles not the leaves.
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Neomorph


Registered: 12/10/14
Posts: 767
Loc: Europe
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#23906062 - 12/08/16 08:26 AM (7 years, 1 month ago) |
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just boiled the bags with water downt to 200 ml
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cleaning dirty cultures with tea agar [Re: Neomorph]
#23906215 - 12/08/16 09:59 AM (7 years, 1 month ago) |
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Here's a malabar culture after cleaning with tea agar. It was pretty dirty from the syringe and was growing very poorly on each transfer from the original plate. This is the first transfer from tea and looks great.
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Leftfield420
bong toker



Registered: 02/26/16
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Re: Cleaning dirty cultures with tea agar [Re: Mycolorado]
#23906244 - 12/08/16 10:12 AM (7 years, 1 month ago) |
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Mold will grow if spores get transfered onto tea agar (waited to long to transfer from a plate with mold), but it does keep bacteria away for the most part...I did a controlled experiment using 1tea agar plate, and 1 PDA plate: took mouth swabs and streaked each plate on 11/27 I have visible bacterial growth in the PDA plate, and not anything that I can see with my naked eye on the tea agar plate
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Leftfield420]
#23906476 - 12/08/16 11:21 AM (7 years, 1 month ago) |
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Quote:
Leftfield420 said: Mold will grow if spores live mold gets transfered onto tea agar.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#23906486 - 12/08/16 11:24 AM (7 years, 1 month ago) |
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Mold spores will still germinate in your spawn tho
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: ComebackKid]
#23906502 - 12/08/16 11:28 AM (7 years, 1 month ago) |
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Working on that one, but normally yes. But not from cooking, mis-handling, filters and so on.
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