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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: Edmunter]
    #23860882 - 11/23/16 02:38 PM (7 years, 2 months ago)

Sandwich works perfectly, katbusa has thankfully tested that method, check the results here.
This was however the older first attempt recipe katbusa developed, credit due.

Both myself and Mycolorado have updated the ratio.


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Re: Cleaning dirty cultures with tea agar [Re: cronicr] * 1
    #23860903 - 11/23/16 02:46 PM (7 years, 2 months ago)

Quote:

cronicr said:
I'm not saying there is anything wrong with it but just like anti biotic agar I would put through a standard recipe before going to grain spawn.



Sorry, yep, after it's looking clean on the tea agar, it should be transferred to and grown out on your standard recipe.


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Re: Cleaning dirty cultures with tea agar [Re: Mycolorado]
    #23860908 - 11/23/16 02:48 PM (7 years, 2 months ago)

You mentioned very slow growth.
I'm assuming there's a reason for the malt extract but for arguments sake, say you took out the 2g ME from the mix. Would the tea have enough nutes to support mycelium?


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Re: Cleaning dirty cultures with tea agar [Re: ComebackKid] * 1
    #23860931 - 11/23/16 02:57 PM (7 years, 2 months ago)

My guess is yes.  Pretty sure Ferather's recipe doesn't use the malt extract.  This is definitely a work in progress and I would encourage experimentation with different percentages, though Ferather is doing a pretty good job dialing it in.


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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: ComebackKid]
    #23860933 - 11/23/16 02:57 PM (7 years, 2 months ago)

Quote:

ComebackKid said:
Would the tea have enough nutes to support mycelium?




Yes, dry tea raw per 100g is richer than 100g dry rye (change to 100g).
The slower growth is due to Ph, not nutrition or energy.



Note the N and protein.


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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
    #23860992 - 11/23/16 03:15 PM (7 years, 2 months ago)

Potential energy and  N sources (incomplete) for tea.

Polyphenols, varied: [ C6 | H5 | O1 ] x.
Cellulose, varied: [ C6 | H10 | O5 ] x.

Theophylline: C7 | H8 | N4 | O2.
Theobromine: C7 | H8 | N4 | O2.
Caffeine: C8 | H10 | N4 | O2.
Theaflavin: C29 | H24 | O12.
Tannins: C76 | H52 | O46.
Catechin: C15 | H14 | O6.
Carotene: C40 | H56.


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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
    #23861008 - 11/23/16 03:21 PM (7 years, 2 months ago)

Quote:

Ferather said:
Quote:

ComebackKid said:
Would the tea have enough nutes to support mycelium?




Yes, dry tea raw per 100g is richer than 100g dry rye (change to 100g).
The slower growth is due to Ph, not nutrition or energy.



Note the N and protein.



Wouldn't a richer sub colonize slower


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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: cronicr]
    #23861021 - 11/23/16 03:24 PM (7 years, 2 months ago)

Funny you say that, that also needs further testing, however various elements and vitamins improve growth speed.


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Re: Cleaning dirty cultures with tea agar [Re: cronicr]
    #23861024 - 11/23/16 03:24 PM (7 years, 2 months ago)

Quote:

cronicr said:
Quote:

Ferather said:
Quote:

ComebackKid said:
Would the tea have enough nutes to support mycelium?




Yes, dry tea raw per 100g is richer than 100g dry rye (change to 100g).
The slower growth is due to Ph, not nutrition or energy.



Note the N and protein.



Wouldn't a richer sub colonize slower




That is my thoughts as well.


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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: Pastywhyte]
    #23861047 - 11/23/16 03:31 PM (7 years, 2 months ago)

Indeed it needs testing, something I will try. Yeast nutrients.

   

Watch what happens, given the right ratio.


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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
    #23862618 - 11/24/16 05:45 AM (7 years, 2 months ago)

Hi Does anyone have a link newer than this or is this fine https://www.shroomery.org/forums/showflat.php/Number/2894662


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OfflineEdmunter
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Re: Cleaning dirty cultures with tea agar [Re: Mycolorado]
    #23862620 - 11/24/16 05:47 AM (7 years, 2 months ago)

Quote:

Mycolorado said:
Just wanted to bring this to the attention of mush cult.  katbusa and Ferather have been experimenting with tea agar to clean up contaminated spawn and cultures.  I thought it would fit in over here as a great alternative to antibiotic agar...tea is readily available and not everyone wants to use antibiotics. Also, bacterial contams are often present when starting from prints and syringes, and can really be discouraging to those starting out.  I modified the original recipe to use less tea as the original amount seemed a bit excessive.  Here are a couple APE cultures and a malabar.  All had some degree of bacterial contam and are now growing clear.  Growth is very slow on this medium but will result in a clean culture. 
NOTE: spores will not germinate on this agar!



200ml H2O
4.25g black tea (2 bags)
2g ME
4g Agar



katbusa's tea agar thread
https://www.shroomery.org/forums/showflat.php/Number/23771470




Does ordinary house hold PG tips work with this?  Uk tea.


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InvisiblePastywhyteMDiscord
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Re: Cleaning dirty cultures with tea agar [Re: Edmunter]
    #23862711 - 11/24/16 06:52 AM (7 years, 2 months ago)

Quote:

Edmunter said:
Hi Does anyone have a link newer than this or is this fine https://www.shroomery.org/forums/showflat.php/Number/2894662




That link is fine but you can skip the peroxide and you don't need gentamiacin to make it work.


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OfflineEdmunter
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Re: Cleaning dirty cultures with tea agar [Re: Pastywhyte]
    #23862720 - 11/24/16 06:56 AM (7 years, 2 months ago)

Quote:

Pastywhyte said:
Quote:

Edmunter said:
Hi Does anyone have a link newer than this or is this fine https://www.shroomery.org/forums/showflat.php/Number/2894662




That link is fine but you can skip the peroxide and you don't need gentamiacin to make it work.




Wait, so im just making an agar plate with no added nutes?


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Re: Cleaning dirty cultures with tea agar [Re: Edmunter]
    #23862724 - 11/24/16 07:01 AM (7 years, 2 months ago)

You need nutes. Just not peroxide or gentamiacin.


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OfflineEdmunter
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Re: Cleaning dirty cultures with tea agar [Re: Pastywhyte]
    #23862752 - 11/24/16 07:14 AM (7 years, 2 months ago)

So just a normal PDA recipe is fine.  Its not clear in that write up.


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InvisibleMycolorado
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Re: Cleaning dirty cultures with tea agar [Re: Edmunter]
    #23862754 - 11/24/16 07:14 AM (7 years, 2 months ago)

Hey Ed, any tea should work (I would stick to unflavored).  Green tea should also be investigated...I think katbusa was testing it.  Hopefully, tea agar will prove effective in helping clean up a variety of contams and peroxide and antibiotics can be avoided.


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Edited by Mycolorado (11/24/16 07:34 AM)


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OfflineEdmunter
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Re: Cleaning dirty cultures with tea agar [Re: Mycolorado]
    #23862791 - 11/24/16 07:30 AM (7 years, 2 months ago)

I am a very modern man and only have fruit teas like passion fruit and mango....... Joking... Thanks Myc I may try both. 

Do you think my P.Sandose looks like it has bacteria as I havent been able to shift the weird growth.  Looks like you turned up just in time.


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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: Edmunter]
    #23862805 - 11/24/16 07:38 AM (7 years, 2 months ago)

I use the extra cheap supermarket stuff. It's more like flour than leaves.
Any unmodified (flavored, e.g. added lemon) raw tea leaves.

For extra benefits balance to Ph 6 using lime.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineEdmunter
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
    #23862811 - 11/24/16 07:41 AM (7 years, 2 months ago)

Quote:

Ferather said:
I use the extra cheap supermarket stuff. It's more like flour than leaves.
Any unmodified (flavored, e.g. added lemon) raw tea leaves.

For extra benefits balance to Ph 6 using lime.





Now youve gone and done it,  The missus wants to know WTF I want litmus paper for........ I blamed you......


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