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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: Bigbadwooof]
#24718796 - 10/18/17 07:10 AM (6 years, 3 months ago) |
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Quote:
Bigbadwooof said: I wonder if gentamycin+tea agar would slow growth too much.
Slow doesn't mean bad, that also means it's working, or at least doing something to restrict growth. I can get amazing genetics after leaving mycelium on a complex substrate (takes 4 months).
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Custer's Chalk Medium (CaCO3 agar), used to identify growth via transparency.
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Ferather's CaCO3 Agar: The sucrose provides glucose and fructose, the soluble nutrients provides nitrogen and other nutrients. Additional proteins, amino acids and other essential nutrients are also provided by the gelatin. 100g > Boiling Hot Water. 2-4g > CaCO3, Calcium Carbonate. 0.1g > MG, Soluble Nutrients. 2g > Sucrose (White Sugar). 0.2g > 240B Gelatin. 2.8g > Agar. Assemble, microwave for 40 seconds. Firmness: Harder than normal agar. Colour: Cream-blue, opaque.

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Calcium carbonate is white-off white, calcium bicarbonate is 100% clear. Mycelium expel CO2, and acids, both react with CaCO3.
Calcium carbonate (insoluble): CaCO3 + CO2 + H2O → Ca(HCO3)2 Calcium hydroxide (insoluble): Ca(OH)2 + CO2 → CaCO3 + H2O
Calcium bicarbonate (soluble): Ca(HCO3)2
Edited by Ferather (10/18/17 08:32 AM)
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infestedpasta
Stranger


Registered: 09/20/07
Posts: 90
Last seen: 3 months, 3 days
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Re: Cleaning dirty cultures with tea agar [Re: Mycolorado]
#27210797 - 02/17/21 09:11 AM (2 years, 11 months ago) |
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Hey I'm a noob here.. Just started working with agar. I have some plates that wont stop transferring this gross slime with the mycelium, so I'm going to give this a shot, seems noob friendly. Hopefully it works I'll let you guys know.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cleaning dirty cultures with tea agar [Re: infestedpasta]
#27210848 - 02/17/21 09:36 AM (2 years, 11 months ago) |
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I had a slime contamination one time from vendor wood spawn, it tried to colonize but didn't do well (plain tea, no ME), I did have to transfer another time but all was well. If you use a strong torch you can visibly see more contamination colonies than just the surface, making it easier to choose locations.

The bleaching (light orange) is white-rot, mycelial enzymes breaking down phenols.
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infestedpasta
Stranger


Registered: 09/20/07
Posts: 90
Last seen: 3 months, 3 days
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
#27210930 - 02/17/21 10:31 AM (2 years, 11 months ago) |
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I just added some tea to grain water agar.. hopefully it works.
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