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OfflineFerather
Mycological
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Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Cleaning dirty cultures with tea agar [Re: Bigbadwooof]
    #24718796 - 10/18/17 07:10 AM (6 years, 3 months ago)

Quote:

Bigbadwooof said:
I wonder if gentamycin+tea agar would slow growth too much.




Slow doesn't mean bad, that also means it's working, or at least doing something to restrict growth.
I can get amazing genetics after leaving mycelium on a complex substrate (takes 4 months).

----

Custer's Chalk Medium (CaCO3 agar), used to identify growth via transparency.

----

Ferather's CaCO3 Agar:

The sucrose provides glucose and fructose, the soluble nutrients provides nitrogen and other nutrients.
Additional proteins, amino acids and other essential nutrients are also provided by the gelatin.


100g > Boiling Hot Water.

2-4g > CaCO3, Calcium Carbonate.
0.1g > MG, Soluble Nutrients.
2g > Sucrose (White Sugar).
0.2g > 240B Gelatin.
2.8g > Agar.

Assemble, microwave for 40 seconds.


Firmness: Harder than normal agar.
Colour: Cream-blue, opaque.





----

Calcium carbonate is white-off white, calcium bicarbonate is 100% clear.
Mycelium expel CO2, and acids, both react with CaCO3.


Calcium carbonate (insoluble): CaCO3 + CO2 + H2O → Ca(HCO3)2
Calcium hydroxide (insoluble): Ca(OH)2 + CO2 → CaCO3 + H2O

Calcium bicarbonate (soluble): Ca(HCO3)2


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Edited by Ferather (10/18/17 08:32 AM)


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Offlineinfestedpasta
Stranger
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Registered: 09/20/07
Posts: 90
Last seen: 3 months, 3 days
Re: Cleaning dirty cultures with tea agar [Re: Mycolorado]
    #27210797 - 02/17/21 09:11 AM (2 years, 11 months ago)

Hey I'm a noob here.. Just started working with agar. I have some plates that wont stop transferring this gross slime with the mycelium, so I'm going to give this a shot, seems noob friendly. Hopefully it works I'll let you guys know.


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OfflineFerather
Mycological
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Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Cleaning dirty cultures with tea agar [Re: infestedpasta]
    #27210848 - 02/17/21 09:36 AM (2 years, 11 months ago)

I had a slime contamination one time from vendor wood spawn, it tried to colonize but didn't do well (plain tea, no ME), I did have to transfer another time but all was well.
If you use a strong torch you can visibly see more contamination colonies than just the surface, making it easier to choose locations.

               

The bleaching (light orange) is white-rot, mycelial enzymes breaking down phenols.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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Offlineinfestedpasta
Stranger
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Registered: 09/20/07
Posts: 90
Last seen: 3 months, 3 days
Re: Cleaning dirty cultures with tea agar [Re: Ferather]
    #27210930 - 02/17/21 10:31 AM (2 years, 11 months ago)

I just added some tea to grain water agar.. hopefully it works.


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