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AK1000
Stranger



Registered: 06/06/16
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Last seen: 2 years, 5 months
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Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough?
#23860160 - 11/23/16 10:01 AM (7 years, 2 months ago) |
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The link to the tek I'm referring to is this by Munchauzen here: https://www.shroomery.org/forums/showflat.php/Number/22833471
He doesn't specify how to make the sterile water so I'll tell you what I did:
I got a few cups of tap water and boiled it vigorously for at least 15 minutes on high heat.
As that was happening I thoroughly washed a plastic quart jar (ziplock twist and lock) with antibacterial soap a couple times inside and out including the lid.
After the 15 minutes I poured some hot water in the plastic jar, closed the lid, shook it up and down so that the hot water would hopefully kill anything the soap didn't get, then poured all that water out and poured in a tiny amount of the hot water (quarter cup or less) and put hte lid on.
I waited for it to cool down and prepared my SAB (and myself) and eventually plopped the agar into the jar and shook the shit out of it. Then I used a splash to inoculate my bottles using the bottle tek. Did I do all right?
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: AK1000]
#23860203 - 11/23/16 10:18 AM (7 years, 2 months ago) |
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The safest way to get sterile water is to PC some water in a jar for 15-20 minutes. I pc it with my grain jars/bags or agar sometimes. So I always have sterile water around.
Your method could have worked but with LC/LI I am always paranoid.
Better safe than sorry.
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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: enlightenment]
#23860241 - 11/23/16 10:38 AM (7 years, 2 months ago) |
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Yeah, I was trying to search how to make sterile water and users were either saying PC-ing it is the foolproof way to go, while others were laughing about PC-ing water and that boiling it for 10 minutes minimum was enough. Something so basic wasn't easy to figure out so I just winged it.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: AK1000]
#23860319 - 11/23/16 11:06 AM (7 years, 2 months ago) |
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You have to sterilize the jar with the water the same way you sterilize your agar (I assume you use a PC for that?). Steam-sterilizing would also work but what you did looks sketchy af. Soap won't kill shit and the boiling water you poured (in open air?) won't do much either if the jar has some sneaky bacteria in it.
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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: Josex]
#23860377 - 11/23/16 11:42 AM (7 years, 2 months ago) |
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Why wouldn't soap kill shit?
But yes, what I did was not the best.
I just didn't want to PC something again that night, but I guess i'll do it in the future.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: AK1000]
#23860406 - 11/23/16 11:59 AM (7 years, 2 months ago) |
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Oh well, I missed it was bacterial soap but I don't think that could compare with proper sterilization, especially if you did the cleaning and the pouring of hot water in open air, that's just a half-ass lazy way to do things, it could work but why risk all your previous agar work?
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: AK1000]
#23860430 - 11/23/16 12:10 PM (7 years, 2 months ago) |
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I wish the best for you, but that might not have worked. You fergot to pc it. Post future bottles even if it turns green. (It might just)
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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AK1000
Stranger



Registered: 06/06/16
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Last seen: 2 years, 5 months
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: wtfcrazymofo]
#23862978 - 11/24/16 08:50 AM (7 years, 2 months ago) |
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True. Well. Another lesson learned. I wish I could just boil and do the transfer in the SAB and that would be much easier. But I guess i'll just sterilize water in the PC on the next batch.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: Josex]
#23863016 - 11/24/16 09:09 AM (7 years, 2 months ago) |
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Quote:
Josex said: Oh well, I missed it was bacterial soap but I don't think that could compare with proper sterilization, especially if you did the cleaning and the pouring of hot water in open air, that's just a half-ass lazy way to do things, it could work but why risk all your previous agar work?
all soap is "[anti]bacterial soap" labelling it as such is just marketing.
the jar has to be sterilized, not cleaned. you sterilize things with a PC. in this case, you could steam it as well.
boiling water and working in open air is a fool-proof method to grow molds and bacteria
Edited by Munchauzen (11/24/16 09:14 AM)
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AK1000
Stranger



Registered: 06/06/16
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: Munchauzen]
#23863600 - 11/24/16 12:56 PM (7 years, 2 months ago) |
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Quote:
Munchauzen said:
boiling water and working in open air is a fool-proof method to grow molds and bacteria
I think just using straight up tap water without boiling would actually be fool proof 
Anyway. Will let you know how badly the bottles come out.
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AK1000
Stranger



Registered: 06/06/16
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Last seen: 2 years, 5 months
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: AK1000]
#23909033 - 12/09/16 01:49 AM (7 years, 1 month ago) |
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Quote:
wtfcrazymofo said: I wish the best for you, but that might not have worked. You fergot to pc it. Post future bottles even if it turns green. (It might just)

Yep. I got 100% Contamination rate with the stupid way that I went with. Do not just boil water. Lesson learned.
Since then, I've inoc'd 28 bottles with the blenderless LI, with water that was PC'd and I've got ZERO contamination, w00t!
Edited by AK1000 (12/09/16 01:50 AM)
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: AK1000]
#23909088 - 12/09/16 03:15 AM (7 years, 1 month ago) |
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Quote:
AK1000 said:
Quote:
wtfcrazymofo said: I wish the best for you, but that might not have worked. You fergot to pc it. Post future bottles even if it turns green. (It might just)

Yep. I got 100% Contamination rate with the stupid way that I went with. Do not just boil water. Lesson learned.
Since then, I've inoc'd 28 bottles with the blenderless LI, with water that was PC'd and I've got ZERO contamination, w00t!
You have some serious balls to post your results, I'll give you that. By doing it you're simply acknowledging publicly the profound retardedness of sacrificing lots of time and work for the sake of skipping a 20 min pc cycle to sterilize a bit of water.
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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: Josex]
#23909538 - 12/09/16 09:09 AM (7 years, 1 month ago) |
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Everyone makes stupid mistakes. It's part of being human, has nothing to do with balls. Anyone who pretends otherwise is full of it.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: AK1000] 2
#23909566 - 12/09/16 09:26 AM (7 years, 1 month ago) |
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Dude I used to work with took 180F water in a bucket put foil over the top and threw it in a walk in cooler to make "sterile room temperature water"
Try explaining that to someone who already knew everything
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: bodhisatta]
#23909687 - 12/09/16 10:22 AM (7 years, 1 month ago) |
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Quote:
bodhisatta said: Dude I used to work with took 180F water in a bucket put foil over the top and threw it in a walk in cooler to make "sterile room temperature water"
Try explaining that to someone who already knew everything
Lmao those are the guys showing up at the shop asking for blinker fluid
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egobrain


Registered: 11/17/16
Posts: 180
Loc: Canada
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Re: Last night I made the blenderless liquid inoculant, can you LMK if I was sterile enough? [Re: bodhisatta]
#23909706 - 12/09/16 10:28 AM (7 years, 1 month ago) |
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Quote:
bodhisatta said: Dude I used to work with took 180F water in a bucket put foil over the top and threw it in a walk in cooler to make "sterile room temperature water"
Try explaining that to someone who already knew everything
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