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OfflineHerdna
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Registered: 04/03/14
Posts: 38
Last seen: 7 years, 2 months
Agar or LC?
    #23859606 - 11/23/16 06:06 AM (7 years, 3 months ago)

Hello again i have been reading some now and i dont know what im going to do!
Can i use Agar and LC together?

And can i use Agar with spore water? im using Spore Vials 10ml.
i read that Agar = no contaminations, and how do i mix the spore water with the Agar, same as LC?

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Offlineenlightenment
alchemist
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Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
Re: Agar or LC? [Re: Herdna]
    #23859654 - 11/23/16 06:39 AM (7 years, 3 months ago)

Quote:

i read that Agar = no contaminations, and how do i mix the spore water with the Agar, same as LC?




Agar = no contaminations? :laugh:
You must have read something wrong.
It is easier to spot a contamination on agar.
You can not see if a LC is clean or not until you test it.

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OfflineTh3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 2 months, 23 days
Re: Agar or LC? [Re: enlightenment]
    #23859667 - 11/23/16 06:49 AM (7 years, 3 months ago)

You got things a little mixed up man. It's ok, you're hear to learn!

There are many means of getting some clean mycelium to work with and  ways to germinate those spores from your syringe.

You can get some agar and make some plates (look for teks).

You can make lc and li (look for teks).

You can also inoculate jars straight from your syringe.


I believe what you are asking is what is the safest way to germinate your spores. Am I correct? If so,
Make some agar plates and drop just one drop into about 3 plates. Wait a week or so for growth and then cut away healthy mycelium and transfer a few times until you only see healthy mycelium.

From there you can make a lc or inoculate your grain jars.

You need to use the search function and do some more reasurch, I think that gives you a starting point tho man, good luck!

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Offlinespore-ty
Male


Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 11 months
Re: Agar or LC? [Re: Th3Issu3]
    #23859669 - 11/23/16 06:51 AM (7 years, 3 months ago)

Quote:

Th3Issu3 said:
You got things a little mixed up man. It's ok, you're hear to learn!

There are many means of getting some clean mycelium to work with and  ways to germinate those spores from your syringe.

You can get some agar and make some plates (look for teks).

You can make lc and li (look for teks).

You can also inoculate jars straight from your syringe.


I believe what you are asking is what is the safest way to germinate your spores. Am I correct? If so,
Make some agar plates and drop just one drop into about 3 plates. Wait a week or so for growth and then cut away healthy mycelium and transfer a few times until you only see healthy mycelium.

From there you can make a lc or inoculate your grain jars.

You need to use the search function and do some more reasurch, I think that gives you a starting point tho man, good luck!




:whathesaid:

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OfflineHerdna
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Registered: 04/03/14
Posts: 38
Last seen: 7 years, 2 months
Re: Agar or LC? [Re: Th3Issu3]
    #23859703 - 11/23/16 07:12 AM (7 years, 3 months ago)

well thanks for the help! :smile:

My plan is to use 2 plastic totes (2x Monotubes) dont know the volyme yet. For the casing im thinking about using coconut coir and vermiculite. For the substrate i will use rye grain.

Im also using

Edited by Herdna (11/23/16 07:13 AM)

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Offlineenlightenment
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Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
Re: Agar or LC? [Re: Herdna]
    #23859713 - 11/23/16 07:20 AM (7 years, 3 months ago)

Suck up some of the spore solution with a sterile syringe with an unused needle. At this point you have a spore syringe.

As Th3Issu3 already wrote read some TEKs before you start.
You should read about how to prepare and sterilize grains.
Good luck. We are always ready to help but you should get some basic knowledge to avoid disappointment. :goodluck:

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OfflineHerdna
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Registered: 04/03/14
Posts: 38
Last seen: 7 years, 2 months
Re: Agar or LC? [Re: enlightenment]
    #23859741 - 11/23/16 07:42 AM (7 years, 3 months ago)

Quote:

enlightenment said:
Suck up some of the spore solution with a sterile syringe with an unused needle. At this point you have a spore syringe.

As Th3Issu3 already wrote read some TEKs before you start.
You should read about how to prepare and sterilize grains.
Good luck. We are always ready to help but you should get some basic knowledge to avoid disappointment. :goodluck:





I allready have one syringe and i burn the needle before i use it.
i have been reading some basics but im still getting confused! haha i have adhd-asbergers so sometimes the text confuses me.

aha i think i get some of it now! i need to transfer the spore solution from the syringe to the agar before it turns into gele when it cools down?

Edited by Herdna (11/23/16 07:52 AM)

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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 5 months
Re: Agar or LC? [Re: Herdna]
    #23859819 - 11/23/16 08:15 AM (7 years, 3 months ago)

read this - the basics

you got pretty much all basic elements to growing mixed up.

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