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noppie
Stranger
Registered: 06/05/09
Posts: 122
Last seen: 4 years, 4 months
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Hydrogen Peroxide (h2o2) soaking jars tek.
#23859310 - 11/23/16 01:42 AM (7 years, 4 months ago) |
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What if we soak our bulk substrates right before casing with h2o2 for avoid possible contaminations?
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afflictis
ModestMycologist
Registered: 03/09/12
Posts: 334
Last seen: 5 years, 11 months
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Re: Hydrogen Peroxide (h2o2) soaking jars tek. [Re: noppie]
#23859336 - 11/23/16 02:10 AM (7 years, 4 months ago) |
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I don't think this would work otherwise it would be posted on most teks
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r.lutece
gave Columbia her wings.
Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: Hydrogen Peroxide (h2o2) soaking jars tek. [Re: afflictis]
#23859345 - 11/23/16 02:15 AM (7 years, 4 months ago) |
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H2O2 really hinders the mycelium. If you soak in H2O2, I'd guess that it would take a fair amount of time for the myc to rebound. During that time, you'd probably see more contams enter the fruiting chamber before the first flush anyway.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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tump
ban the undead
Registered: 03/17/16
Posts: 2,383
Last seen: 7 years, 21 days
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Re: Hydrogen Peroxide (h2o2) soaking jars tek. [Re: r.lutece]
#23859379 - 11/23/16 03:06 AM (7 years, 4 months ago) |
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H2o2 in agar is about as far as im willing to go in mushroom teks. It doesn't save you in anyway. Sterility technical skills will save you. Not h2o2. I once saw a cool clone of wood lover done on cardboard soaked in it but it wasnt needed.
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 2 months
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Re: Hydrogen Peroxide (h2o2) soaking jars tek. [Re: tump]
#23859383 - 11/23/16 03:07 AM (7 years, 4 months ago) |
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I've never used it in agar..wouldn't it just break down right away?
-------------------- It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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enlightenment
alchemist
Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 10 months, 19 days
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Re: Hydrogen Peroxide (h2o2) soaking jars tek. [Re: cronicr]
#23859392 - 11/23/16 03:15 AM (7 years, 4 months ago) |
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It was hyped a lot in this community a few years ago. I never used it, too. So no experience. H2o2 can take some heat. Heat would not be the problem. But no experience here.
Two quotes (~3 years old thread)
Quote:
The thing most people don't realize is that peroxide breaks down very fast into water and oxygen in the presence of organic materials such as agar and malt. If one thinks peroxide put on agar more than an hour or two ago is still 'working' they'd be mistaken.
I mostly speak against dipping cultures in peroxide before placing on agar because it stunts them, leading to slower growth which makes it easier for competitor fungi to get the upper hand. RR
Quote:
Another thing I didn't mention above is that peroxide doesn't necessarily kill spores if it's diluted into agar, but simply keeps them from germinating. On agar, we want any contaminants to germinate so we can transfer our healthy mycelium to a new dish. Peroxidated agar tends to bite one later when the cultures are spawned to grains and those dormant spores begin to germinate.
Storing cultures upside down also causes the moisture to leave the agar by gravity over time. This will cause your culture to dry out, and then get soaked when you turn it over again to work. RR
Edited by enlightenment (11/23/16 03:29 AM)
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 2 months
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Re: Hydrogen Peroxide (h2o2) soaking jars tek. [Re: enlightenment]
#23859438 - 11/23/16 03:52 AM (7 years, 4 months ago) |
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Yeah kinda thought so lol
-------------------- It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Hydrogen Peroxide (h2o2) soaking jars tek. [Re: cronicr]
#23859498 - 11/23/16 04:44 AM (7 years, 4 months ago) |
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Thats a terrible idea... Why would you dunk a substrate in h2O2? You mean like trying to save it after its contaminated? Or ...?
-------------------- Q&A US vs. THEM The more I learn, the less I know.
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tump
ban the undead
Registered: 03/17/16
Posts: 2,383
Last seen: 7 years, 21 days
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Re: Hydrogen Peroxide (h2o2) soaking jars tek. [Re: LocN9ne]
#23859508 - 11/23/16 04:57 AM (7 years, 4 months ago) |
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Maybe he want to make it fizzy like soda
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WeavieWonder
Time Travel Sucks
Registered: 08/18/16
Posts: 1,455
Loc: USA
Last seen: 3 months, 1 day
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Re: Hydrogen Peroxide (h2o2) soaking jars tek. [Re: tump]
#23859622 - 11/23/16 06:16 AM (7 years, 4 months ago) |
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Clean spawn and properly prepared substrate need not these things.
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ComebackKid
Multispore Enthusiast
Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Hydrogen Peroxide (h2o2) soaking jars tek. [Re: noppie]
#23859634 - 11/23/16 06:26 AM (7 years, 4 months ago) |
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Here's a really awesome tek that uses H202. I've been meaning to give it a shot for some time now. I have a feeling it will work perfectly for what you're trying to do
-------------------- Substrate surface conditions / Monotub prep and care Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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