|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 6 months
|
What's the best way to get a clone from a MS syringe if I'm using agar for reliability?
#23797192 - 11/03/16 10:49 AM (7 years, 3 months ago) |
|
|
So, when I started using agar, I started with a MS syringe and kept isolating and after many transfers, I was getting very uniform growth, and then when I tried to grow this on grain, it never fruited, so I'm guessing I got unlucky and isolated non-fruiting genetics.
Now, I've read some comments that say they rather start with a clone (a pin?) and then use that for hte agar instead to ensure that it will fruit anyway. So how do I go about doing that if i'm starting with a MS syringe and agar? Do I just put one drop of spore solution from the syringe in the middle of the agar, let that germinate/grow and then do just ONE transfer to ensure clean growth one more time and then tiger-drop that into some grain and hope it fruits? Then take the first pin I see and put that on some agar and culture that repeatedly?
|
pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,179
Loc: rural ghetto
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
#23797196 - 11/03/16 10:52 AM (7 years, 3 months ago) |
|
|
sounds right...
ms fruits to agar to grain spawn to fruiting again
--------------------
Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 4 months, 27 days
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: pablokabute]
#23797218 - 11/03/16 11:03 AM (7 years, 3 months ago) |
|
|
If you only clean it up, and not isolate, it'll pretty much be guarenteed to fruit.
-------------------- Bug
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 5 months
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: thebug76]
#23797247 - 11/03/16 11:12 AM (7 years, 3 months ago) |
|
|
transfer until clean growth, let plate sit until it starts pinning, clone said pin on new agar plate, et voila!
|
NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 2 months, 4 days
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: spacechildo]
#23797269 - 11/03/16 11:18 AM (7 years, 3 months ago) |
|
|
one of my favorite cultures came from an agar pin
--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: NDStepp84]
#23797326 - 11/03/16 11:35 AM (7 years, 3 months ago) |
|
|
|
AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 6 months
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: bodhisatta]
#23797352 - 11/03/16 11:44 AM (7 years, 3 months ago) |
|
|
Wait, I could have pins form directly on agar? I'm using pasty-plates using the agar recipe he provides, if that matters. How would I initiate pinning on agar?
|
Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 4 months, 3 days
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
#23797363 - 11/03/16 11:45 AM (7 years, 3 months ago) |
|
|
It only ever happens for me when the plate has been fully colonized for a while and it doesn't happen with all of my cultures.
Here's Pan. cyan. pinning on a petri:

Here is a very contaminated no-pour pinning:
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
#23797380 - 11/03/16 11:52 AM (7 years, 3 months ago) |
|
|
Quote:
AK1000 said: How would I initiate pinning on agar?
We don't initiate pinning. The mycelium does. Patience is all you can do
|
AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 6 months
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: bodhisatta]
#23797392 - 11/03/16 11:56 AM (7 years, 3 months ago) |
|
|
Quote:
bodhisatta said:
Quote:
AK1000 said: How would I initiate pinning on agar?
We don't initiate pinning. The mycelium does. Patience is all you can do
Good to know. Would you guys recommend I wait for an agar pin or transfer it to grain and hope for the best? I will be using Madafaka's Bottle Tek to create some clones/mushies cause I got everything necessary to make the coir based bottles now and am kinda excited about that. I just haven't made LI before, though and was thinking of tiger-dropping.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 5 months
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: spacechildo]
#23797507 - 11/03/16 12:28 PM (7 years, 3 months ago) |
|
|
Quote:
spacechildo said: transfer until clean growth, let plate sit until it starts pinning, clone said pin on new agar plate, et voila!
|
AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 6 months
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: spacechildo]
#23858747 - 11/22/16 08:52 PM (7 years, 3 months ago) |
|
|
Quote:
spacechildo said:
Quote:
spacechildo said: transfer until clean growth, let plate sit until it starts pinning, clone said pin on new agar plate, et voila!
Welp, I went on vacation and came back to find not only an agar pin but an entire mushroom. Now what do I do with this?

It looks beautiful and it dropped its spores on the agar, hehe.
|
khatinthehat
Stranger
Registered: 11/22/16
Posts: 4
Last seen: 7 years, 3 months
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
#23858935 - 11/22/16 10:25 PM (7 years, 3 months ago) |
|
|
If you get a fruit like the above pic on agar you better believe thats a good fruiting strain.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,819
Loc: Canada
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: khatinthehat]
#23859108 - 11/22/16 11:50 PM (7 years, 3 months ago) |
|
|
Clone that shit
|
NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 2 months, 4 days
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: Pastywhyte]
#23859113 - 11/22/16 11:53 PM (7 years, 3 months ago) |
|
|
--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
|
AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 6 months
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: Pastywhyte]
#23860100 - 11/23/16 09:42 AM (7 years, 3 months ago) |
|
|
Quote:
Pastywhyte said: Clone that shit
To clarify, should I simply pick it, then cut open the middle of it with a scalpel and put a piece of the inner tissue on some agar? I've never cloned a pin or mushroom before. I've only used agar for transfers pretty much thus far.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
#23860108 - 11/23/16 09:44 AM (7 years, 3 months ago) |
|
|
That's basically it. There's videos by RR and munch if you want too
|
AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 6 months
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: khatinthehat]
#23904937 - 12/07/16 08:00 PM (7 years, 2 months ago) |
|
|
Quote:
khatinthehat said: If you get a fruit like the above pic on agar you better believe thats a good fruiting strain.
I found an old pasty plate that was supposed to be contaminated. Turns out it had created more than a few pins!

|
Bernard
Cultvr - Not Necessarily Trusted



Registered: 05/08/16
Posts: 226
Last seen: 2 years, 8 months
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
#23904968 - 12/07/16 08:10 PM (7 years, 2 months ago) |
|
|
I'm a noob, but I thought that a better way to go about it was to start with something like a PF TEK BRF cake. That way, you get a much better idea what you are cloning (size, potency, etc.).
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,819
Loc: Canada
|
Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: Bernard]
#23904988 - 12/07/16 08:15 PM (7 years, 2 months ago) |
|
|
A clone is always going to need testing etc. But a smaller amount of substrate colonized means fewer cell divisions. Which means more vigor.
|
|