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Offlineguardx23
Stranger

Registered: 11/22/16
Posts: 2
Last seen: 7 years, 25 days
Agar tissue culture ( Please help)
    #23858642 - 11/22/16 08:19 PM (7 years, 2 months ago)

So here is the situation I'm in right now:

I was given a work place where there is no ventilation and only a sliding window for air exchange and can close and lock. There is also an air-con and refrigerator in the room. I am still new to agar tissue culture. Here are some questions i would like to ask from the community:

1. What is the optimum room temperature inside an inoculating room? given i have an air-con to use.

2. what are the things i should consider when working inside an inoculating room? Is it okay to use bleach solution 10% to clean and disinfect the room? or use Lysol? ALso, I am multi task so i move in and out of laboratory should I spray myself with lysol every time i go inside inoculation room? should i place foot bath at the door? I wanted to lessen contamination if possible. can you guys give me advices. thanks.

3. After sterilizing both agar and petri dishes which is the best way to store them while waiting for the agar to be colonized? given i have fridge to use. If using fridge to store what is the optimum temperature for it? Btw im going to be using OYSTER MUSHROOM.


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Agar tissue culture ( Please help) [Re: guardx23]
    #23858897 - 11/22/16 10:09 PM (7 years, 2 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:49 PM)


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Offlineguardx23
Stranger

Registered: 11/22/16
Posts: 2
Last seen: 7 years, 25 days
Re: Agar tissue culture ( Please help) [Re: amidogen]
    #23858955 - 11/22/16 10:37 PM (7 years, 2 months ago)

Yea kinda. My boss asked me to work on mushrooms and im trying to gather much information i can. Unfortunately, i don't have experience on mycology and stuffs.

I am planning to grow it on petri dishes. Thanks tho i will try to check out that one.


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InvisibleMoabfighter
Tam Fighter
 User Gallery

Registered: 12/13/15
Posts: 2,710
Re: Agar tissue culture ( Please help) [Re: guardx23]
    #23858972 - 11/22/16 10:44 PM (7 years, 2 months ago)

What the fuck are you talking about?


--------------------
KSSS And PE WBS.
 


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Agar tissue culture ( Please help) [Re: Moabfighter]
    #23859035 - 11/22/16 11:17 PM (7 years, 2 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:49 PM)


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InvisibleMoabfighter
Tam Fighter
 User Gallery

Registered: 12/13/15
Posts: 2,710
Re: Agar tissue culture ( Please help) [Re: amidogen]
    #23859056 - 11/22/16 11:31 PM (7 years, 2 months ago)

Yeah somehow the poster above me made enough sense of it to say what I was thinking.


Having no mushrooms experience, you're not gonna make anything worth a fuck for your "'manager" in less than a year.

Mycology isn't something your brain is already somewhat wired for. It's like nothing you've ever done before. You totally have to learn from ground zero. It doesn't happen overnight.

I'm a year in and am just now getting SLIGHTLY comfortable with my skills in this hobby. It's hard. It's real fucking hard. The real motherfuckers around here who are good, they still get problems.

Anyone can toss a football. Not everyone can hit a golf ball.


We are a different breed of people, and it takes a decent chunk of your brain energy to learn how to do this.


Good luck.


--------------------
KSSS And PE WBS.
 


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OfflineTmethylM
Smear in the shale
 User Gallery

Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
Trusted Cultivator
Re: Agar tissue culture ( Please help) [Re: amidogen]
    #23859140 - 11/23/16 12:05 AM (7 years, 2 months ago)

Quote:

amidogen said:
Start with RR's Let's Grow Mushrooms! videos for sure.




Yes. Do that.
Direct link below:
http://mushroomvideos.com/

And read this when you get the basics down.

https://www.shroomery.org/forums/showflat.php/Number/17231150/fpart/1

Quote:

amidogen said:
You need to do muchos researchos.



Your Spanish is incredible. :lol:
mucha investigación, or mucho investigación


--------------------
¯\_(ツ)_/¯


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Agar tissue culture ( Please help) [Re: Tmethyl]
    #23859700 - 11/23/16 07:11 AM (7 years, 2 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (06/21/18 11:27 AM)


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Offlinespacechildo
proletarians rise up
Male User Gallery

Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: Agar tissue culture ( Please help) [Re: amidogen]
    #23859925 - 11/23/16 08:53 AM (7 years, 2 months ago)

1: doesnt matter
2: still air
3: on a shelf/table etc.


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